Experiment: BHIS with Doxycycline hyclate 0.000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1675 and BT1676 overlap by 6 nucleotides BT1676 and BT1677 are separated by 30 nucleotides BT1677 and BT1678 overlap by 20 nucleotides
BT1675: BT1675 - conserved protein, with a conserved TPR domain (NCBI ptt file), at 2,067,782 to 2,069,986
BT1675
BT1676: BT1676 - Maf protein (NCBI ptt file), at 2,069,981 to 2,070,562
BT1676
BT1677: BT1677 - putative hydrolase (NCBI ptt file), at 2,070,593 to 2,071,114
BT1677
BT1678: BT1678 - conserved hypothetical protein (NCBI ptt file), at 2,071,095 to 2,071,889
BT1678
Position (kb)
2069
2070
2071 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2069.254 kb on + strand, within BT1675 at 2069.587 kb on + strand, within BT1675 at 2069.798 kb on + strand at 2070.030 kb on - strand at 2070.031 kb on + strand at 2070.034 kb on + strand at 2070.035 kb on - strand at 2070.035 kb on - strand at 2070.052 kb on + strand, within BT1676 at 2070.060 kb on - strand, within BT1676 at 2070.067 kb on + strand, within BT1676 at 2070.067 kb on + strand, within BT1676 at 2070.069 kb on + strand, within BT1676 at 2070.070 kb on - strand, within BT1676 at 2070.121 kb on - strand, within BT1676 at 2070.132 kb on + strand, within BT1676 at 2070.188 kb on - strand, within BT1676 at 2070.188 kb on - strand, within BT1676 at 2070.214 kb on + strand, within BT1676 at 2070.331 kb on + strand, within BT1676 at 2070.403 kb on - strand, within BT1676 at 2070.474 kb on + strand, within BT1676 at 2070.475 kb on - strand, within BT1676 at 2070.531 kb on - strand at 2070.531 kb on - strand at 2070.532 kb on + strand at 2070.533 kb on - strand at 2070.576 kb on - strand at 2070.587 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM remove 2,069,254 + BT1675 0.67 -1.6 2,069,587 + BT1675 0.82 -0.6 2,069,798 + -2.0 2,070,030 - -1.0 2,070,031 + +0.5 2,070,034 + -1.0 2,070,035 - -1.0 2,070,035 - -1.6 2,070,052 + BT1676 0.12 -1.8 2,070,060 - BT1676 0.14 -0.0 2,070,067 + BT1676 0.15 -1.0 2,070,067 + BT1676 0.15 -3.0 2,070,069 + BT1676 0.15 +0.6 2,070,070 - BT1676 0.15 -2.6 2,070,121 - BT1676 0.24 -1.6 2,070,132 + BT1676 0.26 -2.3 2,070,188 - BT1676 0.36 -3.2 2,070,188 - BT1676 0.36 -3.6 2,070,214 + BT1676 0.40 -2.0 2,070,331 + BT1676 0.60 -1.2 2,070,403 - BT1676 0.73 -3.8 2,070,474 + BT1676 0.85 -0.0 2,070,475 - BT1676 0.85 -1.0 2,070,531 - -3.8 2,070,531 - -2.2 2,070,532 + -0.7 2,070,533 - -5.3 2,070,576 - +2.3 2,070,587 + -1.6
Or see this region's nucleotide sequence