Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1422

Experiment: BHIS with Doxycycline hyclate 0.000390625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1420 and BT1421 are separated by 86 nucleotidesBT1421 and BT1422 are separated by 196 nucleotidesBT1422 and BT1423 are separated by 2 nucleotidesBT1423 and BT1424 are separated by 25 nucleotides BT1420: BT1420 - conserved hypothetical protein (NCBI ptt file), at 1,753,097 to 1,755,253 BT1420 BT1421: BT1421 - hypothetical protein (NCBI ptt file), at 1,755,340 to 1,755,654 BT1421 BT1422: BT1422 - conserved hypothetical protein (NCBI ptt file), at 1,755,851 to 1,756,195 BT1422 BT1423: BT1423 - conserved hypothetical protein (NCBI ptt file), at 1,756,198 to 1,756,584 BT1423 BT1424: BT1424 - hypothetical protein (NCBI ptt file), at 1,756,610 to 1,757,227 BT1424 Position (kb) 1755 1756 1757Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1754.948 kb on + strand, within BT1420at 1755.013 kb on + strand, within BT1420at 1755.013 kb on + strand, within BT1420at 1755.169 kb on + strandat 1755.170 kb on - strandat 1755.170 kb on - strandat 1755.212 kb on + strandat 1755.238 kb on + strandat 1755.272 kb on + strandat 1755.301 kb on + strandat 1755.302 kb on - strandat 1755.337 kb on + strandat 1755.341 kb on + strandat 1755.408 kb on + strand, within BT1421at 1755.408 kb on + strand, within BT1421at 1755.485 kb on + strand, within BT1421at 1755.610 kb on + strand, within BT1421at 1755.623 kb on - strandat 1755.626 kb on + strandat 1755.626 kb on + strandat 1755.650 kb on + strandat 1755.851 kb on - strandat 1755.870 kb on + strandat 1755.871 kb on - strandat 1755.933 kb on + strand, within BT1422at 1755.966 kb on + strand, within BT1422at 1756.021 kb on - strand, within BT1422at 1756.045 kb on - strand, within BT1422at 1756.125 kb on + strand, within BT1422at 1756.126 kb on - strand, within BT1422at 1756.138 kb on + strand, within BT1422at 1756.138 kb on + strand, within BT1422at 1756.182 kb on + strandat 1756.182 kb on + strandat 1756.185 kb on + strandat 1756.190 kb on - strandat 1756.220 kb on + strandat 1756.221 kb on - strandat 1756.221 kb on - strandat 1756.221 kb on - strandat 1756.347 kb on + strand, within BT1423at 1756.347 kb on + strand, within BT1423at 1756.352 kb on + strand, within BT1423at 1756.353 kb on - strand, within BT1423at 1756.421 kb on + strand, within BT1423at 1756.422 kb on - strand, within BT1423at 1756.422 kb on - strand, within BT1423at 1756.479 kb on - strand, within BT1423at 1756.479 kb on - strand, within BT1423at 1756.515 kb on - strand, within BT1423at 1756.519 kb on - strand, within BT1423at 1756.569 kb on - strandat 1756.579 kb on - strandat 1756.592 kb on - strandat 1756.632 kb on + strandat 1756.742 kb on + strand, within BT1424at 1756.948 kb on + strand, within BT1424at 1756.960 kb on + strand, within BT1424at 1757.016 kb on + strand, within BT1424at 1757.016 kb on + strand, within BT1424at 1757.017 kb on - strand, within BT1424at 1757.063 kb on + strand, within BT1424at 1757.075 kb on + strand, within BT1424at 1757.105 kb on + strand, within BT1424at 1757.117 kb on - strand, within BT1424at 1757.133 kb on + strand, within BT1424at 1757.182 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM
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1,754,948 + BT1420 0.86 +1.5
1,755,013 + BT1420 0.89 +0.6
1,755,013 + BT1420 0.89 -3.3
1,755,169 + +0.7
1,755,170 - -0.6
1,755,170 - -2.6
1,755,212 + -1.0
1,755,238 + -0.3
1,755,272 + -2.0
1,755,301 + -1.6
1,755,302 - -2.0
1,755,337 + +0.0
1,755,341 + +0.0
1,755,408 + BT1421 0.22 +0.8
1,755,408 + BT1421 0.22 -1.3
1,755,485 + BT1421 0.46 -0.1
1,755,610 + BT1421 0.86 +0.2
1,755,623 - +0.0
1,755,626 + +1.6
1,755,626 + +0.0
1,755,650 + +1.0
1,755,851 - -0.8
1,755,870 + +0.6
1,755,871 - -0.4
1,755,933 + BT1422 0.24 +0.5
1,755,966 + BT1422 0.33 -2.3
1,756,021 - BT1422 0.49 -4.0
1,756,045 - BT1422 0.56 -2.3
1,756,125 + BT1422 0.79 -2.0
1,756,126 - BT1422 0.80 -3.0
1,756,138 + BT1422 0.83 -2.0
1,756,138 + BT1422 0.83 -2.6
1,756,182 + -1.2
1,756,182 + +0.0
1,756,185 + +0.3
1,756,190 - -2.0
1,756,220 + -2.3
1,756,221 - -0.1
1,756,221 - -1.0
1,756,221 - -1.0
1,756,347 + BT1423 0.39 +3.2
1,756,347 + BT1423 0.39 -1.0
1,756,352 + BT1423 0.40 -0.2
1,756,353 - BT1423 0.40 +0.0
1,756,421 + BT1423 0.58 -0.2
1,756,422 - BT1423 0.58 -1.9
1,756,422 - BT1423 0.58 -1.6
1,756,479 - BT1423 0.73 +2.6
1,756,479 - BT1423 0.73 +3.0
1,756,515 - BT1423 0.82 +0.0
1,756,519 - BT1423 0.83 -2.8
1,756,569 - +0.0
1,756,579 - +0.0
1,756,592 - -2.2
1,756,632 + -0.6
1,756,742 + BT1424 0.21 -0.6
1,756,948 + BT1424 0.55 -0.4
1,756,960 + BT1424 0.57 -1.6
1,757,016 + BT1424 0.66 +0.0
1,757,016 + BT1424 0.66 +0.4
1,757,017 - BT1424 0.66 -1.6
1,757,063 + BT1424 0.73 +0.4
1,757,075 + BT1424 0.75 +1.3
1,757,105 + BT1424 0.80 -1.6
1,757,117 - BT1424 0.82 -1.9
1,757,133 + BT1424 0.85 -3.3
1,757,182 + +0.0

Or see this region's nucleotide sequence