Experiment: BHIS with Doxycycline hyclate 0.000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1283 and BT1284 are separated by 27 nucleotides BT1284 and BT1285 are separated by 196 nucleotides
BT1283: BT1283 - conserved hypothetical protein (NCBI ptt file), at 1,596,492 to 1,597,814
BT1283
BT1284: BT1284 - putative endo-beta-N-acetylglucosaminidase F1 precursor (mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F1) (NCBI ptt file), at 1,597,842 to 1,599,368
BT1284
BT1285: BT1285 - endo-beta-N-acetylglucosaminidase (NCBI ptt file), at 1,599,565 to 1,600,485
BT1285
Position (kb)
1597
1598
1599
1600 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1596.947 kb on - strand, within BT1283 at 1596.972 kb on + strand, within BT1283 at 1596.972 kb on + strand, within BT1283 at 1596.972 kb on + strand, within BT1283 at 1596.972 kb on + strand, within BT1283 at 1597.133 kb on + strand, within BT1283 at 1597.158 kb on - strand, within BT1283 at 1597.327 kb on - strand, within BT1283 at 1597.417 kb on + strand, within BT1283 at 1597.479 kb on + strand, within BT1283 at 1597.480 kb on - strand, within BT1283 at 1597.494 kb on + strand, within BT1283 at 1597.494 kb on + strand, within BT1283 at 1597.496 kb on + strand, within BT1283 at 1597.813 kb on - strand at 1597.842 kb on - strand at 1597.862 kb on + strand at 1597.862 kb on + strand at 1597.922 kb on + strand at 1597.923 kb on - strand at 1597.926 kb on - strand at 1597.959 kb on - strand at 1597.960 kb on + strand at 1597.960 kb on + strand at 1597.960 kb on + strand at 1597.971 kb on - strand at 1597.976 kb on + strand at 1597.976 kb on + strand at 1597.981 kb on + strand at 1597.981 kb on + strand at 1598.017 kb on + strand, within BT1284 at 1598.160 kb on + strand, within BT1284 at 1598.160 kb on + strand, within BT1284 at 1598.176 kb on + strand, within BT1284 at 1598.183 kb on - strand, within BT1284 at 1598.240 kb on + strand, within BT1284 at 1598.283 kb on + strand, within BT1284 at 1598.288 kb on - strand, within BT1284 at 1598.353 kb on - strand, within BT1284 at 1598.353 kb on - strand, within BT1284 at 1598.356 kb on - strand, within BT1284 at 1598.360 kb on - strand, within BT1284 at 1598.363 kb on + strand, within BT1284 at 1598.364 kb on - strand, within BT1284 at 1598.401 kb on + strand, within BT1284 at 1598.530 kb on + strand, within BT1284 at 1598.590 kb on + strand, within BT1284 at 1598.590 kb on + strand, within BT1284 at 1598.607 kb on + strand, within BT1284 at 1598.612 kb on - strand, within BT1284 at 1598.653 kb on + strand, within BT1284 at 1598.741 kb on - strand, within BT1284 at 1598.789 kb on + strand, within BT1284 at 1598.791 kb on + strand, within BT1284 at 1598.792 kb on - strand, within BT1284 at 1598.879 kb on + strand, within BT1284 at 1598.900 kb on - strand, within BT1284 at 1598.904 kb on + strand, within BT1284 at 1598.904 kb on + strand, within BT1284 at 1599.005 kb on + strand, within BT1284 at 1599.006 kb on - strand, within BT1284 at 1599.079 kb on + strand, within BT1284 at 1599.089 kb on + strand, within BT1284 at 1599.089 kb on + strand, within BT1284 at 1599.091 kb on + strand, within BT1284 at 1599.091 kb on + strand, within BT1284 at 1599.091 kb on + strand, within BT1284 at 1599.091 kb on + strand, within BT1284 at 1599.095 kb on + strand, within BT1284 at 1599.096 kb on - strand, within BT1284 at 1599.116 kb on + strand, within BT1284 at 1599.157 kb on + strand, within BT1284 at 1599.184 kb on + strand, within BT1284 at 1599.190 kb on + strand, within BT1284 at 1599.207 kb on + strand, within BT1284 at 1599.207 kb on + strand, within BT1284 at 1599.208 kb on - strand, within BT1284 at 1599.232 kb on - strand at 1599.245 kb on + strand at 1599.252 kb on + strand at 1599.366 kb on + strand at 1599.382 kb on + strand at 1599.386 kb on + strand at 1599.434 kb on - strand at 1599.437 kb on + strand at 1599.488 kb on + strand at 1599.524 kb on - strand at 1599.657 kb on + strand, within BT1285 at 1599.696 kb on + strand, within BT1285 at 1599.722 kb on + strand, within BT1285 at 1599.754 kb on + strand, within BT1285 at 1599.756 kb on + strand, within BT1285 at 1599.784 kb on + strand, within BT1285 at 1599.784 kb on + strand, within BT1285 at 1599.788 kb on + strand, within BT1285 at 1599.788 kb on + strand, within BT1285 at 1599.789 kb on - strand, within BT1285 at 1599.789 kb on - strand, within BT1285 at 1599.818 kb on + strand, within BT1285 at 1599.832 kb on - strand, within BT1285 at 1599.832 kb on - strand, within BT1285 at 1599.836 kb on + strand, within BT1285 at 1599.854 kb on + strand, within BT1285 at 1599.859 kb on + strand, within BT1285 at 1599.933 kb on - strand, within BT1285 at 1599.933 kb on - strand, within BT1285 at 1600.028 kb on + strand, within BT1285 at 1600.028 kb on + strand, within BT1285 at 1600.029 kb on - strand, within BT1285 at 1600.093 kb on - strand, within BT1285 at 1600.107 kb on + strand, within BT1285 at 1600.143 kb on + strand, within BT1285 at 1600.157 kb on + strand, within BT1285 at 1600.157 kb on + strand, within BT1285 at 1600.197 kb on + strand, within BT1285 at 1600.197 kb on + strand, within BT1285 at 1600.203 kb on + strand, within BT1285 at 1600.204 kb on - strand, within BT1285 at 1600.285 kb on + strand, within BT1285 at 1600.321 kb on - strand, within BT1285 at 1600.353 kb on + strand, within BT1285
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM remove 1,596,947 - BT1283 0.34 -1.0 1,596,972 + BT1283 0.36 +1.6 1,596,972 + BT1283 0.36 +0.6 1,596,972 + BT1283 0.36 -0.0 1,596,972 + BT1283 0.36 -1.0 1,597,133 + BT1283 0.48 -1.6 1,597,158 - BT1283 0.50 -0.3 1,597,327 - BT1283 0.63 -3.0 1,597,417 + BT1283 0.70 -0.9 1,597,479 + BT1283 0.75 -3.3 1,597,480 - BT1283 0.75 -1.0 1,597,494 + BT1283 0.76 -3.2 1,597,494 + BT1283 0.76 -1.4 1,597,496 + BT1283 0.76 -1.0 1,597,813 - -0.0 1,597,842 - -4.3 1,597,862 + -1.6 1,597,862 + -0.8 1,597,922 + -1.0 1,597,923 - -0.5 1,597,926 - -2.3 1,597,959 - -1.0 1,597,960 + -2.3 1,597,960 + +0.2 1,597,960 + -0.5 1,597,971 - +0.4 1,597,976 + -1.0 1,597,976 + -0.0 1,597,981 + -2.0 1,597,981 + -0.5 1,598,017 + BT1284 0.11 -2.0 1,598,160 + BT1284 0.21 -2.0 1,598,160 + BT1284 0.21 -2.0 1,598,176 + BT1284 0.22 -1.6 1,598,183 - BT1284 0.22 +0.5 1,598,240 + BT1284 0.26 -1.5 1,598,283 + BT1284 0.29 +1.8 1,598,288 - BT1284 0.29 -1.1 1,598,353 - BT1284 0.33 -2.3 1,598,353 - BT1284 0.33 -1.6 1,598,356 - BT1284 0.34 +0.2 1,598,360 - BT1284 0.34 -2.3 1,598,363 + BT1284 0.34 -1.6 1,598,364 - BT1284 0.34 +1.3 1,598,401 + BT1284 0.37 -2.8 1,598,530 + BT1284 0.45 -1.4 1,598,590 + BT1284 0.49 -1.6 1,598,590 + BT1284 0.49 -2.0 1,598,607 + BT1284 0.50 -0.0 1,598,612 - BT1284 0.50 -2.2 1,598,653 + BT1284 0.53 -0.5 1,598,741 - BT1284 0.59 -1.6 1,598,789 + BT1284 0.62 -3.7 1,598,791 + BT1284 0.62 -2.6 1,598,792 - BT1284 0.62 -0.6 1,598,879 + BT1284 0.68 -3.1 1,598,900 - BT1284 0.69 -2.0 1,598,904 + BT1284 0.70 -2.8 1,598,904 + BT1284 0.70 -1.1 1,599,005 + BT1284 0.76 -1.8 1,599,006 - BT1284 0.76 -1.0 1,599,079 + BT1284 0.81 -1.6 1,599,089 + BT1284 0.82 -0.0 1,599,089 + BT1284 0.82 -0.0 1,599,091 + BT1284 0.82 -1.6 1,599,091 + BT1284 0.82 -2.3 1,599,091 + BT1284 0.82 -3.2 1,599,091 + BT1284 0.82 -3.3 1,599,095 + BT1284 0.82 -3.8 1,599,096 - BT1284 0.82 -1.6 1,599,116 + BT1284 0.83 -1.6 1,599,157 + BT1284 0.86 -0.4 1,599,184 + BT1284 0.88 -1.6 1,599,190 + BT1284 0.88 -0.0 1,599,207 + BT1284 0.89 -1.8 1,599,207 + BT1284 0.89 -1.6 1,599,208 - BT1284 0.89 -0.0 1,599,232 - +0.3 1,599,245 + -1.0 1,599,252 + -2.8 1,599,366 + -1.0 1,599,382 + -0.0 1,599,386 + -1.6 1,599,434 - -0.0 1,599,437 + -1.0 1,599,488 + -1.4 1,599,524 - -0.0 1,599,657 + BT1285 0.10 -2.0 1,599,696 + BT1285 0.14 -0.0 1,599,722 + BT1285 0.17 -1.9 1,599,754 + BT1285 0.21 -1.6 1,599,756 + BT1285 0.21 +0.2 1,599,784 + BT1285 0.24 -3.3 1,599,784 + BT1285 0.24 -1.3 1,599,788 + BT1285 0.24 -0.0 1,599,788 + BT1285 0.24 -1.0 1,599,789 - BT1285 0.24 -2.0 1,599,789 - BT1285 0.24 +1.4 1,599,818 + BT1285 0.27 -1.5 1,599,832 - BT1285 0.29 -0.0 1,599,832 - BT1285 0.29 +0.3 1,599,836 + BT1285 0.29 +1.0 1,599,854 + BT1285 0.31 -2.8 1,599,859 + BT1285 0.32 -0.0 1,599,933 - BT1285 0.40 +0.7 1,599,933 - BT1285 0.40 -1.6 1,600,028 + BT1285 0.50 -1.0 1,600,028 + BT1285 0.50 -1.2 1,600,029 - BT1285 0.50 -1.1 1,600,093 - BT1285 0.57 +1.3 1,600,107 + BT1285 0.59 -3.5 1,600,143 + BT1285 0.63 -1.6 1,600,157 + BT1285 0.64 -2.8 1,600,157 + BT1285 0.64 -1.0 1,600,197 + BT1285 0.69 -0.8 1,600,197 + BT1285 0.69 -0.0 1,600,203 + BT1285 0.69 -2.6 1,600,204 - BT1285 0.69 -3.3 1,600,285 + BT1285 0.78 -0.0 1,600,321 - BT1285 0.82 -3.0 1,600,353 + BT1285 0.86 -0.3
Or see this region's nucleotide sequence