Experiment: BHIS with Doxycycline hyclate 0.000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1076 and tRNA-Asp are separated by 137 nucleotides tRNA-Asp and BT1077 are separated by 157 nucleotides BT1077 and BT1078 are separated by 150 nucleotides BT1078 and BT1079 overlap by 4 nucleotides
BT1076: BT1076 - two-component system response regulator (NCBI ptt file), at 1,362,814 to 1,363,593
BT1076
BT_t19: tRNA-Asp - tRNA-Asp (RefSeq), at 1,363,731 to 1,363,804
tRNA-Asp
BT1077: BT1077 - putative aspartate aminotransferase (NCBI ptt file), at 1,363,962 to 1,365,278
BT1077
BT1078: BT1078 - putative transcriptional regulator (NCBI ptt file), at 1,365,429 to 1,366,019
BT1078
BT1079: BT1079 - acetyltransferase (NCBI ptt file), at 1,366,016 to 1,366,555
BT1079
Position (kb)
1363
1364
1365
1366 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1363.092 kb on + strand, within BT1076 at 1363.115 kb on + strand, within BT1076 at 1363.132 kb on - strand, within BT1076 at 1363.256 kb on - strand, within BT1076 at 1363.328 kb on - strand, within BT1076 at 1363.454 kb on + strand, within BT1076 at 1363.541 kb on + strand at 1363.562 kb on + strand at 1363.625 kb on + strand at 1363.886 kb on - strand at 1364.244 kb on - strand, within BT1077 at 1364.304 kb on - strand, within BT1077 at 1364.410 kb on + strand, within BT1077 at 1364.504 kb on + strand, within BT1077 at 1364.505 kb on - strand, within BT1077 at 1364.517 kb on - strand, within BT1077 at 1364.712 kb on + strand, within BT1077 at 1364.749 kb on - strand, within BT1077 at 1364.871 kb on + strand, within BT1077 at 1364.908 kb on - strand, within BT1077 at 1364.974 kb on + strand, within BT1077 at 1364.974 kb on + strand, within BT1077 at 1365.034 kb on + strand, within BT1077 at 1365.132 kb on + strand, within BT1077 at 1365.133 kb on - strand, within BT1077 at 1365.198 kb on - strand at 1365.206 kb on + strand at 1365.287 kb on + strand at 1365.400 kb on - strand at 1365.400 kb on - strand at 1365.467 kb on + strand at 1365.623 kb on + strand, within BT1078 at 1365.639 kb on + strand, within BT1078 at 1365.640 kb on - strand, within BT1078 at 1365.640 kb on - strand, within BT1078 at 1365.661 kb on - strand, within BT1078 at 1365.686 kb on + strand, within BT1078 at 1365.686 kb on + strand, within BT1078 at 1365.704 kb on + strand, within BT1078 at 1365.704 kb on + strand, within BT1078 at 1365.704 kb on + strand, within BT1078 at 1365.705 kb on - strand, within BT1078 at 1365.787 kb on + strand, within BT1078 at 1365.788 kb on - strand, within BT1078 at 1365.938 kb on + strand, within BT1078 at 1365.964 kb on + strand at 1365.965 kb on - strand at 1365.975 kb on + strand at 1365.975 kb on + strand at 1365.976 kb on - strand at 1366.017 kb on + strand at 1366.200 kb on + strand, within BT1079
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM remove 1,363,092 + BT1076 0.36 -0.1 1,363,115 + BT1076 0.39 -0.1 1,363,132 - BT1076 0.41 -2.6 1,363,256 - BT1076 0.57 -2.5 1,363,328 - BT1076 0.66 -2.9 1,363,454 + BT1076 0.82 -1.5 1,363,541 + -1.7 1,363,562 + -1.7 1,363,625 + +0.3 1,363,886 - -3.3 1,364,244 - BT1077 0.21 -1.7 1,364,304 - BT1077 0.26 -2.7 1,364,410 + BT1077 0.34 +0.8 1,364,504 + BT1077 0.41 +0.5 1,364,505 - BT1077 0.41 +1.0 1,364,517 - BT1077 0.42 -0.1 1,364,712 + BT1077 0.57 -0.1 1,364,749 - BT1077 0.60 -2.7 1,364,871 + BT1077 0.69 -0.1 1,364,908 - BT1077 0.72 -3.8 1,364,974 + BT1077 0.77 +1.1 1,364,974 + BT1077 0.77 -2.7 1,365,034 + BT1077 0.81 -3.5 1,365,132 + BT1077 0.89 +0.6 1,365,133 - BT1077 0.89 -2.1 1,365,198 - -0.3 1,365,206 + +4.1 1,365,287 + +0.9 1,365,400 - -1.1 1,365,400 - -1.0 1,365,467 + -2.2 1,365,623 + BT1078 0.33 -0.1 1,365,639 + BT1078 0.36 -2.4 1,365,640 - BT1078 0.36 -2.4 1,365,640 - BT1078 0.36 -0.1 1,365,661 - BT1078 0.39 +1.8 1,365,686 + BT1078 0.43 +0.1 1,365,686 + BT1078 0.43 -1.1 1,365,704 + BT1078 0.47 -0.1 1,365,704 + BT1078 0.47 +1.1 1,365,704 + BT1078 0.47 +0.4 1,365,705 - BT1078 0.47 +0.4 1,365,787 + BT1078 0.61 -0.1 1,365,788 - BT1078 0.61 -0.3 1,365,938 + BT1078 0.86 +0.1 1,365,964 + -1.1 1,365,965 - +0.3 1,365,975 + -0.5 1,365,975 + -0.1 1,365,976 - -3.5 1,366,017 + -0.1 1,366,200 + BT1079 0.34 -3.1
Or see this region's nucleotide sequence