Experiment: BHIS with Doxycycline hyclate 0.000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0290 and BT0291 are separated by 202 nucleotides BT0291 and BT0292 are separated by 391 nucleotides
BT0290: BT0290 - beta-galactosidase precursor (NCBI ptt file), at 337,216 to 339,555
BT0290
BT0291: BT0291 - integrase (NCBI ptt file), at 339,758 to 340,723
BT0291
BT0292: BT0292 - conserved hypothetical protein (NCBI ptt file), at 341,115 to 341,864
BT0292
Position (kb)
339
340
341 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 338.768 kb on - strand, within BT0290 at 338.869 kb on - strand, within BT0290 at 338.875 kb on - strand, within BT0290 at 338.938 kb on + strand, within BT0290 at 338.939 kb on - strand, within BT0290 at 338.939 kb on - strand, within BT0290 at 338.939 kb on - strand, within BT0290 at 338.983 kb on - strand, within BT0290 at 339.021 kb on - strand, within BT0290 at 339.067 kb on + strand, within BT0290 at 339.135 kb on + strand, within BT0290 at 339.395 kb on - strand at 339.395 kb on - strand at 339.472 kb on + strand at 339.477 kb on - strand at 339.510 kb on + strand at 339.589 kb on - strand at 339.737 kb on - strand at 339.745 kb on - strand at 339.745 kb on - strand at 339.749 kb on - strand at 339.755 kb on + strand at 339.846 kb on - strand at 339.889 kb on + strand, within BT0291 at 339.890 kb on - strand, within BT0291 at 339.909 kb on + strand, within BT0291 at 339.970 kb on + strand, within BT0291 at 339.986 kb on - strand, within BT0291 at 339.991 kb on + strand, within BT0291 at 339.991 kb on + strand, within BT0291 at 340.070 kb on - strand, within BT0291 at 340.201 kb on + strand, within BT0291 at 340.303 kb on - strand, within BT0291 at 340.373 kb on + strand, within BT0291 at 340.377 kb on + strand, within BT0291 at 340.514 kb on + strand, within BT0291 at 340.966 kb on - strand at 341.024 kb on - strand at 341.031 kb on - strand at 341.102 kb on - strand at 341.102 kb on - strand at 341.106 kb on - strand at 341.162 kb on - strand at 341.334 kb on - strand, within BT0292 at 341.343 kb on - strand, within BT0292 at 341.425 kb on - strand, within BT0292 at 341.425 kb on - strand, within BT0292 at 341.425 kb on - strand, within BT0292 at 341.425 kb on - strand, within BT0292 at 341.466 kb on - strand, within BT0292 at 341.516 kb on - strand, within BT0292 at 341.581 kb on + strand, within BT0292 at 341.625 kb on - strand, within BT0292 at 341.638 kb on - strand, within BT0292
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM remove 338,768 - BT0290 0.66 -2.9 338,869 - BT0290 0.71 -0.5 338,875 - BT0290 0.71 +1.5 338,938 + BT0290 0.74 -0.1 338,939 - BT0290 0.74 -0.5 338,939 - BT0290 0.74 -0.1 338,939 - BT0290 0.74 -0.7 338,983 - BT0290 0.76 -1.7 339,021 - BT0290 0.77 -0.9 339,067 + BT0290 0.79 -1.1 339,135 + BT0290 0.82 -1.1 339,395 - -1.6 339,395 - -0.9 339,472 + +0.6 339,477 - +1.3 339,510 + -1.6 339,589 - -1.0 339,737 - -1.6 339,745 - -1.6 339,745 - -0.1 339,749 - -2.4 339,755 + -1.6 339,846 - +1.8 339,889 + BT0291 0.14 -3.2 339,890 - BT0291 0.14 -0.1 339,909 + BT0291 0.16 -1.1 339,970 + BT0291 0.22 -2.6 339,986 - BT0291 0.24 +0.5 339,991 + BT0291 0.24 -2.9 339,991 + BT0291 0.24 -3.4 340,070 - BT0291 0.32 -1.3 340,201 + BT0291 0.46 -1.6 340,303 - BT0291 0.56 -2.1 340,373 + BT0291 0.64 -2.1 340,377 + BT0291 0.64 +2.7 340,514 + BT0291 0.78 -3.4 340,966 - +1.5 341,024 - -1.4 341,031 - -0.0 341,102 - -2.4 341,102 - -1.0 341,106 - -0.0 341,162 - -0.5 341,334 - BT0292 0.29 -0.5 341,343 - BT0292 0.30 -1.0 341,425 - BT0292 0.41 -4.3 341,425 - BT0292 0.41 -2.6 341,425 - BT0292 0.41 -1.0 341,425 - BT0292 0.41 +0.8 341,466 - BT0292 0.47 -2.0 341,516 - BT0292 0.53 -3.0 341,581 + BT0292 0.62 -0.0 341,625 - BT0292 0.68 -3.2 341,638 - BT0292 0.70 +1.0
Or see this region's nucleotide sequence