Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0190

Experiment: BHIS with Doxycycline hyclate 0.000390625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0189 and BT0190 are separated by 212 nucleotidesBT0190 and BT0191 are separated by 23 nucleotides BT0189: BT0189 - conserved hypothetical protein, putative anti-sigma factor (NCBI ptt file), at 198,928 to 199,911 BT0189 BT0190: BT0190 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 200,124 to 203,564 BT0190 BT0191: BT0191 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 203,588 to 204,979 BT0191 Position (kb) 200 201 202 203 204Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 199.153 kb on - strand, within BT0189at 199.240 kb on - strand, within BT0189at 199.240 kb on - strand, within BT0189at 199.240 kb on - strand, within BT0189at 199.528 kb on - strand, within BT0189at 199.554 kb on - strand, within BT0189at 199.554 kb on - strand, within BT0189at 199.626 kb on - strand, within BT0189at 199.711 kb on - strand, within BT0189at 199.752 kb on + strand, within BT0189at 199.753 kb on - strand, within BT0189at 199.818 kb on + strandat 199.839 kb on + strandat 199.871 kb on - strandat 199.901 kb on - strandat 199.917 kb on - strandat 200.069 kb on - strandat 200.203 kb on + strandat 200.204 kb on - strandat 200.246 kb on + strandat 200.247 kb on - strandat 200.318 kb on + strandat 200.319 kb on - strandat 200.385 kb on - strandat 200.445 kb on - strandat 200.445 kb on - strandat 200.605 kb on - strand, within BT0190at 200.749 kb on + strand, within BT0190at 200.750 kb on - strand, within BT0190at 200.750 kb on - strand, within BT0190at 200.762 kb on - strand, within BT0190at 201.015 kb on - strand, within BT0190at 201.096 kb on - strand, within BT0190at 201.270 kb on - strand, within BT0190at 201.285 kb on - strand, within BT0190at 201.508 kb on - strand, within BT0190at 201.539 kb on + strand, within BT0190at 201.680 kb on + strandat 201.681 kb on - strand, within BT0190at 201.684 kb on + strand, within BT0190at 201.776 kb on - strand, within BT0190at 202.002 kb on - strand, within BT0190at 202.007 kb on + strand, within BT0190at 202.077 kb on - strand, within BT0190at 202.121 kb on - strand, within BT0190at 202.189 kb on - strand, within BT0190at 202.207 kb on - strand, within BT0190at 202.213 kb on - strand, within BT0190at 202.213 kb on - strand, within BT0190at 202.220 kb on + strand, within BT0190at 202.225 kb on - strand, within BT0190at 202.225 kb on - strand, within BT0190at 202.400 kb on + strand, within BT0190at 202.401 kb on - strand, within BT0190at 202.407 kb on - strand, within BT0190at 202.459 kb on - strand, within BT0190at 202.459 kb on - strand, within BT0190at 202.497 kb on + strand, within BT0190at 202.499 kb on + strand, within BT0190at 202.500 kb on - strand, within BT0190at 202.631 kb on + strand, within BT0190at 202.699 kb on + strand, within BT0190at 202.761 kb on + strand, within BT0190at 202.863 kb on + strand, within BT0190at 202.864 kb on - strand, within BT0190at 202.864 kb on - strand, within BT0190at 202.899 kb on + strand, within BT0190at 203.049 kb on - strand, within BT0190at 203.053 kb on - strand, within BT0190at 203.053 kb on - strand, within BT0190at 203.054 kb on + strand, within BT0190at 203.096 kb on + strand, within BT0190at 203.175 kb on - strand, within BT0190at 203.284 kb on - strandat 203.326 kb on - strandat 203.439 kb on - strandat 203.468 kb on - strandat 203.553 kb on - strandat 203.562 kb on + strandat 203.563 kb on - strandat 203.573 kb on - strandat 203.692 kb on + strandat 203.693 kb on - strandat 203.693 kb on - strandat 203.697 kb on + strandat 203.697 kb on + strandat 203.724 kb on + strandat 203.920 kb on - strand, within BT0191at 203.971 kb on + strand, within BT0191at 203.972 kb on - strand, within BT0191at 203.990 kb on - strand, within BT0191at 204.032 kb on - strand, within BT0191at 204.036 kb on - strand, within BT0191at 204.169 kb on + strand, within BT0191at 204.301 kb on - strand, within BT0191at 204.309 kb on - strand, within BT0191at 204.337 kb on - strand, within BT0191at 204.434 kb on - strand, within BT0191at 204.529 kb on - strand, within BT0191

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM
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199,153 - BT0189 0.23 -0.1
199,240 - BT0189 0.32 -3.1
199,240 - BT0189 0.32 -1.1
199,240 - BT0189 0.32 -0.1
199,528 - BT0189 0.61 -1.1
199,554 - BT0189 0.64 -1.1
199,554 - BT0189 0.64 +1.3
199,626 - BT0189 0.71 -2.4
199,711 - BT0189 0.80 -1.1
199,752 + BT0189 0.84 -0.1
199,753 - BT0189 0.84 -1.1
199,818 + -2.1
199,839 + -1.1
199,871 - -0.1
199,901 - -1.1
199,917 - -1.9
200,069 - -0.1
200,203 + -1.9
200,204 - -0.1
200,246 + -0.4
200,247 - -0.9
200,318 + -2.1
200,319 - -0.1
200,385 - -2.1
200,445 - -1.9
200,445 - -0.1
200,605 - BT0190 0.14 -3.7
200,749 + BT0190 0.18 -2.7
200,750 - BT0190 0.18 -1.3
200,750 - BT0190 0.18 -0.1
200,762 - BT0190 0.19 -1.1
201,015 - BT0190 0.26 -3.1
201,096 - BT0190 0.28 +1.5
201,270 - BT0190 0.33 +0.1
201,285 - BT0190 0.34 -3.9
201,508 - BT0190 0.40 -2.1
201,539 + BT0190 0.41 -1.1
201,680 + -0.1
201,681 - BT0190 0.45 -2.1
201,684 + BT0190 0.45 -0.1
201,776 - BT0190 0.48 -1.1
202,002 - BT0190 0.55 -2.4
202,007 + BT0190 0.55 -2.7
202,077 - BT0190 0.57 -1.8
202,121 - BT0190 0.58 -0.1
202,189 - BT0190 0.60 -1.7
202,207 - BT0190 0.61 -1.2
202,213 - BT0190 0.61 -1.1
202,213 - BT0190 0.61 -1.0
202,220 + BT0190 0.61 -0.1
202,225 - BT0190 0.61 -0.1
202,225 - BT0190 0.61 +0.9
202,400 + BT0190 0.66 -0.1
202,401 - BT0190 0.66 -2.9
202,407 - BT0190 0.66 -0.1
202,459 - BT0190 0.68 -1.7
202,459 - BT0190 0.68 -2.9
202,497 + BT0190 0.69 -1.2
202,499 + BT0190 0.69 -1.7
202,500 - BT0190 0.69 -1.1
202,631 + BT0190 0.73 -0.8
202,699 + BT0190 0.75 -1.7
202,761 + BT0190 0.77 -1.1
202,863 + BT0190 0.80 -1.3
202,864 - BT0190 0.80 -4.2
202,864 - BT0190 0.80 -4.8
202,899 + BT0190 0.81 +2.3
203,049 - BT0190 0.85 -2.4
203,053 - BT0190 0.85 -0.8
203,053 - BT0190 0.85 +0.3
203,054 + BT0190 0.85 -0.1
203,096 + BT0190 0.86 -1.7
203,175 - BT0190 0.89 -0.4
203,284 - -0.9
203,326 - -0.9
203,439 - -1.7
203,468 - -1.1
203,553 - -0.0
203,562 + -1.7
203,563 - -0.1
203,573 - -1.7
203,692 + -1.7
203,693 - -0.9
203,693 - -1.1
203,697 + -1.1
203,697 + +0.1
203,724 + -2.1
203,920 - BT0191 0.24 -2.1
203,971 + BT0191 0.28 -0.1
203,972 - BT0191 0.28 -0.1
203,990 - BT0191 0.29 +0.2
204,032 - BT0191 0.32 -0.2
204,036 - BT0191 0.32 -2.4
204,169 + BT0191 0.42 -0.1
204,301 - BT0191 0.51 -2.7
204,309 - BT0191 0.52 +0.4
204,337 - BT0191 0.54 -0.7
204,434 - BT0191 0.61 -0.3
204,529 - BT0191 0.68 -0.3

Or see this region's nucleotide sequence