Experiment: Biofilm in Fresh BG11R in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ctaD and ctaE are separated by 51 nucleotides ctaE and Synpcc7942_2605 are separated by 62 nucleotides Synpcc7942_2605 and thrS are separated by 270 nucleotides
Synpcc7942_2603: ctaD - Cytochrome-c oxidase, at 2,685,051 to 2,686,676
ctaD
Synpcc7942_2604: ctaE - cytochrome c oxidase subunit III, at 2,686,728 to 2,687,315
ctaE
Synpcc7942_2605: Synpcc7942_2605 - hypothetical protein, at 2,687,378 to 2,688,034
_2605
Synpcc7942_2606: thrS - threonyl-tRNA synthetase, at 2,688,305 to 2,690,119
thrS
Position (kb)
2686
2687
2688 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2685.728 kb on - strand, within ctaD at 2685.735 kb on - strand, within ctaD at 2685.750 kb on + strand, within ctaD at 2685.830 kb on + strand, within ctaD at 2685.830 kb on - strand, within ctaD at 2685.898 kb on - strand, within ctaD at 2685.938 kb on - strand, within ctaD at 2685.988 kb on + strand, within ctaD at 2686.117 kb on - strand, within ctaD at 2686.159 kb on + strand, within ctaD at 2686.159 kb on + strand, within ctaD at 2686.354 kb on + strand, within ctaD at 2686.354 kb on + strand, within ctaD at 2686.354 kb on + strand, within ctaD at 2686.362 kb on - strand, within ctaD at 2686.384 kb on + strand at 2686.389 kb on + strand, within ctaD at 2686.392 kb on - strand, within ctaD at 2686.424 kb on - strand, within ctaD at 2686.472 kb on - strand, within ctaD at 2686.522 kb on + strand at 2686.540 kb on - strand at 2686.543 kb on + strand at 2686.551 kb on - strand at 2686.570 kb on - strand at 2686.713 kb on + strand at 2686.738 kb on - strand at 2686.861 kb on + strand, within ctaE at 2686.946 kb on - strand at 2687.144 kb on - strand, within ctaE at 2687.156 kb on - strand, within ctaE at 2687.406 kb on - strand at 2687.502 kb on + strand, within Synpcc7942_2605 at 2687.502 kb on + strand, within Synpcc7942_2605 at 2687.502 kb on + strand, within Synpcc7942_2605 at 2687.502 kb on + strand, within Synpcc7942_2605 at 2687.510 kb on - strand at 2687.510 kb on - strand, within Synpcc7942_2605 at 2687.653 kb on - strand, within Synpcc7942_2605 at 2687.659 kb on - strand, within Synpcc7942_2605 at 2687.670 kb on + strand, within Synpcc7942_2605 at 2687.697 kb on - strand, within Synpcc7942_2605 at 2687.773 kb on + strand, within Synpcc7942_2605 at 2687.774 kb on + strand, within Synpcc7942_2605 at 2687.837 kb on + strand, within Synpcc7942_2605 at 2687.847 kb on + strand, within Synpcc7942_2605 at 2687.847 kb on + strand at 2687.860 kb on - strand, within Synpcc7942_2605 at 2687.893 kb on + strand, within Synpcc7942_2605 at 2687.901 kb on - strand, within Synpcc7942_2605 at 2687.901 kb on - strand, within Synpcc7942_2605 at 2687.938 kb on - strand, within Synpcc7942_2605 at 2687.939 kb on - strand, within Synpcc7942_2605 at 2687.945 kb on + strand, within Synpcc7942_2605 at 2687.957 kb on - strand, within Synpcc7942_2605 at 2687.975 kb on - strand at 2687.975 kb on - strand at 2687.977 kb on + strand at 2687.977 kb on + strand at 2687.985 kb on - strand at 2687.985 kb on - strand at 2688.002 kb on - strand at 2688.004 kb on - strand at 2688.076 kb on + strand at 2688.084 kb on - strand at 2688.084 kb on - strand at 2688.084 kb on - strand at 2688.086 kb on + strand at 2688.102 kb on + strand at 2688.102 kb on + strand at 2688.189 kb on - strand at 2688.280 kb on + strand at 2688.283 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in Fresh BG11R in Flasks with Spin rep 1 remove 2,685,728 - ctaD Synpcc7942_2603 0.42 +0.9 2,685,735 - ctaD Synpcc7942_2603 0.42 +1.9 2,685,750 + ctaD Synpcc7942_2603 0.43 -0.6 2,685,830 + ctaD Synpcc7942_2603 0.48 +0.1 2,685,830 - ctaD Synpcc7942_2603 0.48 -0.3 2,685,898 - ctaD Synpcc7942_2603 0.52 -0.6 2,685,938 - ctaD Synpcc7942_2603 0.55 -0.6 2,685,988 + ctaD Synpcc7942_2603 0.58 +0.9 2,686,117 - ctaD Synpcc7942_2603 0.66 -0.1 2,686,159 + ctaD Synpcc7942_2603 0.68 +2.9 2,686,159 + ctaD Synpcc7942_2603 0.68 -2.2 2,686,354 + ctaD Synpcc7942_2603 0.80 +0.3 2,686,354 + ctaD Synpcc7942_2603 0.80 +1.9 2,686,354 + ctaD Synpcc7942_2603 0.80 +1.5 2,686,362 - ctaD Synpcc7942_2603 0.81 -2.0 2,686,384 + -0.9 2,686,389 + ctaD Synpcc7942_2603 0.82 +0.9 2,686,392 - ctaD Synpcc7942_2603 0.82 -1.6 2,686,424 - ctaD Synpcc7942_2603 0.84 -0.5 2,686,472 - ctaD Synpcc7942_2603 0.87 +0.9 2,686,522 + -0.8 2,686,540 - +0.7 2,686,543 + -0.8 2,686,551 - +0.6 2,686,570 - +0.2 2,686,713 + -0.6 2,686,738 - +0.9 2,686,861 + ctaE Synpcc7942_2604 0.23 -2.9 2,686,946 - -0.7 2,687,144 - ctaE Synpcc7942_2604 0.71 +0.5 2,687,156 - ctaE Synpcc7942_2604 0.73 -0.4 2,687,406 - -1.5 2,687,502 + Synpcc7942_2605 0.19 +1.0 2,687,502 + Synpcc7942_2605 0.19 -2.2 2,687,502 + Synpcc7942_2605 0.19 -1.4 2,687,502 + Synpcc7942_2605 0.19 -1.8 2,687,510 - -1.1 2,687,510 - Synpcc7942_2605 0.20 +0.9 2,687,653 - Synpcc7942_2605 0.42 +0.9 2,687,659 - Synpcc7942_2605 0.43 -0.8 2,687,670 + Synpcc7942_2605 0.44 -2.4 2,687,697 - Synpcc7942_2605 0.49 -0.6 2,687,773 + Synpcc7942_2605 0.60 -2.3 2,687,774 + Synpcc7942_2605 0.60 -0.9 2,687,837 + Synpcc7942_2605 0.70 +1.4 2,687,847 + Synpcc7942_2605 0.71 -2.0 2,687,847 + +0.7 2,687,860 - Synpcc7942_2605 0.73 -1.4 2,687,893 + Synpcc7942_2605 0.78 +0.9 2,687,901 - Synpcc7942_2605 0.80 -0.1 2,687,901 - Synpcc7942_2605 0.80 +0.9 2,687,938 - Synpcc7942_2605 0.85 -1.4 2,687,939 - Synpcc7942_2605 0.85 +0.9 2,687,945 + Synpcc7942_2605 0.86 -2.3 2,687,957 - Synpcc7942_2605 0.88 +2.9 2,687,975 - +0.9 2,687,975 - -2.4 2,687,977 + +3.6 2,687,977 + -1.4 2,687,985 - +1.5 2,687,985 - -1.5 2,688,002 - -1.5 2,688,004 - -1.4 2,688,076 + -2.2 2,688,084 - -0.6 2,688,084 - -2.9 2,688,084 - -1.6 2,688,086 + -1.9 2,688,102 + -1.4 2,688,102 + -1.8 2,688,189 - -0.8 2,688,280 + -0.6 2,688,283 - +0.4
Or see this region's nucleotide sequence