Experiment: Biofilm in Fresh BG11R in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuD and Synpcc7942_2549 are separated by 72 nucleotides Synpcc7942_2549 and Synpcc7942_2550 are separated by 128 nucleotides Synpcc7942_2550 and hypF are separated by 189 nucleotides
Synpcc7942_2548: leuD - isopropylmalate isomerase small subunit, at 2,627,423 to 2,628,031
leuD
Synpcc7942_2549: Synpcc7942_2549 - hypothetical protein, at 2,628,104 to 2,628,358
_2549
Synpcc7942_2550: Synpcc7942_2550 - hypothetical protein, at 2,628,487 to 2,629,560
_2550
Synpcc7942_2551: hypF - (NiFe) hydrogenase maturation protein HypF, at 2,629,750 to 2,632,062
hypF
Position (kb)
2628
2629
2630 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2628.051 kb on + strand at 2628.051 kb on + strand at 2628.051 kb on + strand at 2628.051 kb on + strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.085 kb on + strand at 2628.090 kb on + strand at 2628.102 kb on + strand at 2628.108 kb on + strand at 2628.187 kb on - strand, within Synpcc7942_2549 at 2628.209 kb on - strand, within Synpcc7942_2549 at 2628.212 kb on + strand, within Synpcc7942_2549 at 2628.222 kb on + strand, within Synpcc7942_2549 at 2628.234 kb on + strand, within Synpcc7942_2549 at 2628.267 kb on + strand, within Synpcc7942_2549 at 2628.284 kb on + strand, within Synpcc7942_2549 at 2628.343 kb on + strand at 2628.343 kb on + strand at 2628.343 kb on + strand at 2628.343 kb on + strand at 2628.343 kb on + strand at 2628.343 kb on + strand at 2628.351 kb on - strand at 2628.351 kb on - strand at 2628.351 kb on - strand at 2628.351 kb on - strand at 2628.351 kb on - strand at 2628.373 kb on + strand at 2628.373 kb on + strand at 2628.391 kb on - strand at 2628.397 kb on + strand at 2628.397 kb on + strand at 2628.405 kb on - strand at 2628.416 kb on + strand at 2628.429 kb on - strand at 2628.433 kb on + strand at 2628.433 kb on + strand at 2628.441 kb on - strand at 2628.441 kb on - strand at 2628.441 kb on - strand at 2628.483 kb on + strand at 2628.500 kb on + strand at 2628.520 kb on + strand at 2628.556 kb on + strand at 2628.564 kb on - strand at 2628.564 kb on - strand at 2628.564 kb on - strand at 2628.566 kb on + strand at 2628.566 kb on + strand at 2628.574 kb on - strand at 2628.574 kb on - strand at 2628.574 kb on - strand at 2628.576 kb on + strand at 2628.617 kb on + strand, within Synpcc7942_2550 at 2628.621 kb on - strand, within Synpcc7942_2550 at 2628.622 kb on + strand, within Synpcc7942_2550 at 2628.622 kb on - strand, within Synpcc7942_2550 at 2628.627 kb on + strand, within Synpcc7942_2550 at 2628.641 kb on + strand, within Synpcc7942_2550 at 2628.641 kb on + strand, within Synpcc7942_2550 at 2628.641 kb on - strand, within Synpcc7942_2550 at 2628.649 kb on - strand, within Synpcc7942_2550 at 2628.716 kb on + strand, within Synpcc7942_2550 at 2628.724 kb on - strand, within Synpcc7942_2550 at 2628.726 kb on + strand, within Synpcc7942_2550 at 2628.771 kb on + strand, within Synpcc7942_2550 at 2628.799 kb on + strand, within Synpcc7942_2550 at 2628.805 kb on - strand, within Synpcc7942_2550 at 2628.805 kb on - strand, within Synpcc7942_2550 at 2628.871 kb on + strand, within Synpcc7942_2550 at 2628.888 kb on + strand, within Synpcc7942_2550 at 2628.910 kb on - strand, within Synpcc7942_2550 at 2628.922 kb on - strand, within Synpcc7942_2550 at 2628.943 kb on - strand, within Synpcc7942_2550 at 2628.946 kb on + strand, within Synpcc7942_2550 at 2628.946 kb on + strand, within Synpcc7942_2550 at 2628.953 kb on - strand at 2628.954 kb on - strand, within Synpcc7942_2550 at 2628.956 kb on - strand, within Synpcc7942_2550 at 2628.969 kb on + strand, within Synpcc7942_2550 at 2628.982 kb on + strand, within Synpcc7942_2550 at 2628.988 kb on - strand, within Synpcc7942_2550 at 2629.014 kb on + strand, within Synpcc7942_2550 at 2629.056 kb on - strand, within Synpcc7942_2550 at 2629.061 kb on + strand, within Synpcc7942_2550 at 2629.069 kb on - strand, within Synpcc7942_2550 at 2629.069 kb on - strand, within Synpcc7942_2550 at 2629.084 kb on + strand, within Synpcc7942_2550 at 2629.151 kb on + strand, within Synpcc7942_2550 at 2629.151 kb on + strand, within Synpcc7942_2550 at 2629.152 kb on + strand at 2629.159 kb on - strand, within Synpcc7942_2550 at 2629.159 kb on - strand, within Synpcc7942_2550 at 2629.159 kb on - strand, within Synpcc7942_2550 at 2629.160 kb on - strand, within Synpcc7942_2550 at 2629.184 kb on - strand, within Synpcc7942_2550 at 2629.189 kb on + strand, within Synpcc7942_2550 at 2629.189 kb on + strand, within Synpcc7942_2550 at 2629.189 kb on + strand, within Synpcc7942_2550 at 2629.189 kb on + strand, within Synpcc7942_2550 at 2629.189 kb on + strand, within Synpcc7942_2550 at 2629.189 kb on + strand, within Synpcc7942_2550 at 2629.197 kb on - strand, within Synpcc7942_2550 at 2629.197 kb on - strand, within Synpcc7942_2550 at 2629.197 kb on - strand, within Synpcc7942_2550 at 2629.197 kb on - strand, within Synpcc7942_2550 at 2629.197 kb on - strand, within Synpcc7942_2550 at 2629.217 kb on - strand, within Synpcc7942_2550 at 2629.225 kb on - strand, within Synpcc7942_2550 at 2629.245 kb on + strand, within Synpcc7942_2550 at 2629.253 kb on - strand, within Synpcc7942_2550 at 2629.370 kb on + strand, within Synpcc7942_2550 at 2629.389 kb on - strand, within Synpcc7942_2550 at 2629.390 kb on - strand, within Synpcc7942_2550 at 2629.404 kb on - strand, within Synpcc7942_2550 at 2629.413 kb on - strand, within Synpcc7942_2550 at 2629.437 kb on + strand, within Synpcc7942_2550 at 2629.445 kb on - strand, within Synpcc7942_2550 at 2629.456 kb on + strand at 2629.456 kb on + strand at 2629.456 kb on + strand at 2629.456 kb on + strand at 2629.464 kb on - strand at 2629.464 kb on - strand at 2629.466 kb on + strand at 2629.474 kb on - strand at 2629.480 kb on + strand at 2629.480 kb on + strand at 2629.485 kb on + strand at 2629.488 kb on - strand at 2629.493 kb on + strand at 2629.504 kb on + strand at 2629.546 kb on - strand at 2629.555 kb on + strand at 2629.568 kb on - strand at 2629.601 kb on - strand at 2629.611 kb on - strand at 2629.637 kb on - strand at 2629.637 kb on - strand at 2629.682 kb on + strand at 2629.682 kb on + strand at 2629.709 kb on + strand at 2629.716 kb on + strand at 2629.717 kb on - strand at 2629.741 kb on - strand at 2629.742 kb on - strand at 2629.755 kb on - strand at 2629.758 kb on - strand at 2629.771 kb on - strand at 2629.792 kb on + strand at 2629.798 kb on - strand at 2629.811 kb on - strand at 2629.829 kb on + strand at 2629.829 kb on + strand at 2629.829 kb on + strand at 2629.829 kb on + strand at 2629.829 kb on + strand at 2629.834 kb on + strand at 2629.837 kb on - strand at 2629.837 kb on - strand at 2629.837 kb on - strand at 2629.837 kb on - strand at 2629.837 kb on - strand at 2629.906 kb on + strand at 2629.933 kb on - strand at 2629.974 kb on + strand at 2629.975 kb on + strand at 2629.975 kb on + strand at 2629.983 kb on - strand, within hypF at 2629.983 kb on - strand, within hypF at 2629.983 kb on - strand, within hypF at 2629.983 kb on - strand, within hypF at 2629.983 kb on - strand, within hypF at 2629.983 kb on - strand, within hypF at 2629.983 kb on - strand, within hypF at 2630.006 kb on - strand, within hypF at 2630.048 kb on + strand, within hypF at 2630.053 kb on + strand, within hypF at 2630.053 kb on + strand, within hypF at 2630.061 kb on - strand, within hypF at 2630.112 kb on - strand, within hypF at 2630.154 kb on - strand, within hypF at 2630.197 kb on - strand, within hypF at 2630.211 kb on + strand, within hypF at 2630.246 kb on - strand at 2630.293 kb on - strand, within hypF at 2630.335 kb on - strand, within hypF at 2630.345 kb on + strand, within hypF at 2630.368 kb on - strand, within hypF at 2630.374 kb on + strand, within hypF at 2630.374 kb on + strand, within hypF at 2630.385 kb on + strand, within hypF at 2630.388 kb on - strand, within hypF at 2630.388 kb on - strand, within hypF at 2630.388 kb on - strand, within hypF at 2630.420 kb on + strand, within hypF at 2630.420 kb on + strand, within hypF at 2630.422 kb on + strand, within hypF at 2630.428 kb on - strand, within hypF at 2630.428 kb on - strand, within hypF at 2630.430 kb on - strand, within hypF at 2630.437 kb on + strand, within hypF at 2630.445 kb on - strand, within hypF at 2630.449 kb on + strand, within hypF at 2630.451 kb on - strand, within hypF at 2630.459 kb on + strand, within hypF at 2630.465 kb on + strand, within hypF at 2630.487 kb on - strand at 2630.492 kb on - strand, within hypF at 2630.529 kb on - strand, within hypF at 2630.531 kb on + strand, within hypF
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in Fresh BG11R in Flasks with Spin rep 1 remove 2,628,051 + +0.9 2,628,051 + -0.6 2,628,051 + -0.0 2,628,051 + +0.3 2,628,059 - +1.7 2,628,059 - -1.2 2,628,059 - -1.2 2,628,059 - -2.0 2,628,059 - -2.4 2,628,059 - -0.7 2,628,085 + -1.3 2,628,090 + +0.9 2,628,102 + -1.4 2,628,108 + +0.2 2,628,187 - Synpcc7942_2549 0.33 -0.6 2,628,209 - Synpcc7942_2549 0.41 -1.1 2,628,212 + Synpcc7942_2549 0.42 -0.6 2,628,222 + Synpcc7942_2549 0.46 -0.5 2,628,234 + Synpcc7942_2549 0.51 -1.0 2,628,267 + Synpcc7942_2549 0.64 +0.9 2,628,284 + Synpcc7942_2549 0.71 +0.9 2,628,343 + -0.6 2,628,343 + +0.9 2,628,343 + -1.4 2,628,343 + -0.1 2,628,343 + -0.4 2,628,343 + -2.8 2,628,351 - -0.1 2,628,351 - -1.3 2,628,351 - -1.4 2,628,351 - -2.9 2,628,351 - +0.1 2,628,373 + -0.9 2,628,373 + +0.9 2,628,391 - -0.8 2,628,397 + +0.6 2,628,397 + +0.8 2,628,405 - +0.7 2,628,416 + +0.9 2,628,429 - +0.9 2,628,433 + -3.5 2,628,433 + -2.8 2,628,441 - -0.1 2,628,441 - -0.9 2,628,441 - -0.6 2,628,483 + -0.2 2,628,500 + -2.9 2,628,520 + -4.6 2,628,556 + +0.1 2,628,564 - +0.6 2,628,564 - -0.3 2,628,564 - +0.9 2,628,566 + +2.7 2,628,566 + +2.4 2,628,574 - +0.9 2,628,574 - -4.0 2,628,574 - -3.3 2,628,576 + +0.4 2,628,617 + Synpcc7942_2550 0.12 -2.5 2,628,621 - Synpcc7942_2550 0.12 -2.5 2,628,622 + Synpcc7942_2550 0.13 +0.7 2,628,622 - Synpcc7942_2550 0.13 -1.3 2,628,627 + Synpcc7942_2550 0.13 -1.9 2,628,641 + Synpcc7942_2550 0.14 -2.0 2,628,641 + Synpcc7942_2550 0.14 -0.5 2,628,641 - Synpcc7942_2550 0.14 -1.7 2,628,649 - Synpcc7942_2550 0.15 -1.6 2,628,716 + Synpcc7942_2550 0.21 -0.8 2,628,724 - Synpcc7942_2550 0.22 -0.6 2,628,726 + Synpcc7942_2550 0.22 +0.7 2,628,771 + Synpcc7942_2550 0.26 -0.1 2,628,799 + Synpcc7942_2550 0.29 -0.8 2,628,805 - Synpcc7942_2550 0.30 -3.1 2,628,805 - Synpcc7942_2550 0.30 -3.0 2,628,871 + Synpcc7942_2550 0.36 -2.1 2,628,888 + Synpcc7942_2550 0.37 -1.9 2,628,910 - Synpcc7942_2550 0.39 +0.4 2,628,922 - Synpcc7942_2550 0.41 -0.1 2,628,943 - Synpcc7942_2550 0.42 +1.9 2,628,946 + Synpcc7942_2550 0.43 -1.6 2,628,946 + Synpcc7942_2550 0.43 -1.7 2,628,953 - -0.6 2,628,954 - Synpcc7942_2550 0.43 +0.4 2,628,956 - Synpcc7942_2550 0.44 -1.0 2,628,969 + Synpcc7942_2550 0.45 -1.1 2,628,982 + Synpcc7942_2550 0.46 -2.6 2,628,988 - Synpcc7942_2550 0.47 -2.1 2,629,014 + Synpcc7942_2550 0.49 +0.9 2,629,056 - Synpcc7942_2550 0.53 -0.7 2,629,061 + Synpcc7942_2550 0.53 -0.9 2,629,069 - Synpcc7942_2550 0.54 -0.1 2,629,069 - Synpcc7942_2550 0.54 -2.2 2,629,084 + Synpcc7942_2550 0.56 +0.9 2,629,151 + Synpcc7942_2550 0.62 -2.1 2,629,151 + Synpcc7942_2550 0.62 -2.4 2,629,152 + -1.4 2,629,159 - Synpcc7942_2550 0.63 -0.6 2,629,159 - Synpcc7942_2550 0.63 -0.2 2,629,159 - Synpcc7942_2550 0.63 -0.6 2,629,160 - Synpcc7942_2550 0.63 -1.4 2,629,184 - Synpcc7942_2550 0.65 -0.6 2,629,189 + Synpcc7942_2550 0.65 -1.4 2,629,189 + Synpcc7942_2550 0.65 -1.4 2,629,189 + Synpcc7942_2550 0.65 +0.9 2,629,189 + Synpcc7942_2550 0.65 -0.8 2,629,189 + Synpcc7942_2550 0.65 +0.2 2,629,189 + Synpcc7942_2550 0.65 -0.4 2,629,197 - Synpcc7942_2550 0.66 -0.3 2,629,197 - Synpcc7942_2550 0.66 +0.1 2,629,197 - Synpcc7942_2550 0.66 -1.5 2,629,197 - Synpcc7942_2550 0.66 +0.9 2,629,197 - Synpcc7942_2550 0.66 -0.1 2,629,217 - Synpcc7942_2550 0.68 -1.9 2,629,225 - Synpcc7942_2550 0.69 +0.2 2,629,245 + Synpcc7942_2550 0.71 +1.9 2,629,253 - Synpcc7942_2550 0.71 -1.2 2,629,370 + Synpcc7942_2550 0.82 +0.9 2,629,389 - Synpcc7942_2550 0.84 -0.4 2,629,390 - Synpcc7942_2550 0.84 -1.1 2,629,404 - Synpcc7942_2550 0.85 +0.9 2,629,413 - Synpcc7942_2550 0.86 -1.2 2,629,437 + Synpcc7942_2550 0.88 -1.4 2,629,445 - Synpcc7942_2550 0.89 -2.0 2,629,456 + -0.1 2,629,456 + -0.2 2,629,456 + -1.1 2,629,456 + -0.1 2,629,464 - -0.6 2,629,464 - -3.1 2,629,466 + +1.4 2,629,474 - -2.9 2,629,480 + -1.7 2,629,480 + +2.5 2,629,485 + -1.1 2,629,488 - +1.7 2,629,493 + +0.9 2,629,504 + +1.9 2,629,546 - -0.9 2,629,555 + +0.9 2,629,568 - +0.6 2,629,601 - -0.9 2,629,611 - -3.1 2,629,637 - -2.6 2,629,637 - +2.1 2,629,682 + -0.1 2,629,682 + +0.4 2,629,709 + +2.0 2,629,716 + -0.8 2,629,717 - +0.9 2,629,741 - -0.8 2,629,742 - -1.7 2,629,755 - +0.7 2,629,758 - -2.9 2,629,771 - +0.1 2,629,792 + -0.9 2,629,798 - +0.4 2,629,811 - -1.6 2,629,829 + -1.8 2,629,829 + -0.1 2,629,829 + +0.9 2,629,829 + +0.9 2,629,829 + +0.8 2,629,834 + -1.5 2,629,837 - +1.2 2,629,837 - -2.6 2,629,837 - +0.4 2,629,837 - -1.3 2,629,837 - -2.0 2,629,906 + +0.9 2,629,933 - +0.1 2,629,974 + -0.0 2,629,975 + -0.9 2,629,975 + -2.6 2,629,983 - hypF Synpcc7942_2551 0.10 -0.9 2,629,983 - hypF Synpcc7942_2551 0.10 -0.9 2,629,983 - hypF Synpcc7942_2551 0.10 -1.1 2,629,983 - hypF Synpcc7942_2551 0.10 +1.5 2,629,983 - hypF Synpcc7942_2551 0.10 -0.3 2,629,983 - hypF Synpcc7942_2551 0.10 -2.0 2,629,983 - hypF Synpcc7942_2551 0.10 -0.9 2,630,006 - hypF Synpcc7942_2551 0.11 -1.1 2,630,048 + hypF Synpcc7942_2551 0.13 +0.9 2,630,053 + hypF Synpcc7942_2551 0.13 -0.6 2,630,053 + hypF Synpcc7942_2551 0.13 -0.6 2,630,061 - hypF Synpcc7942_2551 0.13 -1.1 2,630,112 - hypF Synpcc7942_2551 0.16 +2.9 2,630,154 - hypF Synpcc7942_2551 0.17 -1.9 2,630,197 - hypF Synpcc7942_2551 0.19 -0.1 2,630,211 + hypF Synpcc7942_2551 0.20 -1.1 2,630,246 - -2.1 2,630,293 - hypF Synpcc7942_2551 0.23 -2.0 2,630,335 - hypF Synpcc7942_2551 0.25 +0.8 2,630,345 + hypF Synpcc7942_2551 0.26 +0.9 2,630,368 - hypF Synpcc7942_2551 0.27 -1.9 2,630,374 + hypF Synpcc7942_2551 0.27 -0.8 2,630,374 + hypF Synpcc7942_2551 0.27 +2.5 2,630,385 + hypF Synpcc7942_2551 0.27 -2.8 2,630,388 - hypF Synpcc7942_2551 0.28 -2.1 2,630,388 - hypF Synpcc7942_2551 0.28 -1.3 2,630,388 - hypF Synpcc7942_2551 0.28 +0.5 2,630,420 + hypF Synpcc7942_2551 0.29 +1.0 2,630,420 + hypF Synpcc7942_2551 0.29 -0.7 2,630,422 + hypF Synpcc7942_2551 0.29 +0.7 2,630,428 - hypF Synpcc7942_2551 0.29 -1.3 2,630,428 - hypF Synpcc7942_2551 0.29 -1.1 2,630,430 - hypF Synpcc7942_2551 0.29 -1.4 2,630,437 + hypF Synpcc7942_2551 0.30 +1.6 2,630,445 - hypF Synpcc7942_2551 0.30 -0.1 2,630,449 + hypF Synpcc7942_2551 0.30 -0.6 2,630,451 - hypF Synpcc7942_2551 0.30 +2.1 2,630,459 + hypF Synpcc7942_2551 0.31 +1.9 2,630,465 + hypF Synpcc7942_2551 0.31 -0.6 2,630,487 - +0.9 2,630,492 - hypF Synpcc7942_2551 0.32 +1.8 2,630,529 - hypF Synpcc7942_2551 0.34 +0.9 2,630,531 + hypF Synpcc7942_2551 0.34 +0.4
Or see this region's nucleotide sequence