Experiment: Biofilm in Fresh BG11R in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nblR and Synpcc7942_2306 are separated by 132 nucleotides Synpcc7942_2306 and Synpcc7942_2307 are separated by 3 nucleotides Synpcc7942_2307 and gcvT are separated by 129 nucleotides
Synpcc7942_2305: nblR - two component transcriptional regulator, winged helix family, at 2,373,315 to 2,374,001
nblR
Synpcc7942_2306: Synpcc7942_2306 - Heat shock protein DnaJ-like, at 2,374,134 to 2,374,721
_2306
Synpcc7942_2307: Synpcc7942_2307 - hypothetical protein, at 2,374,725 to 2,375,735
_2307
Synpcc7942_2308: gcvT - glycine cleavage system aminomethyltransferase T, at 2,375,865 to 2,376,983
gcvT
Position (kb)
2374
2375
2376 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2373.869 kb on - strand, within nblR at 2373.973 kb on + strand at 2374.025 kb on + strand at 2374.038 kb on - strand at 2374.084 kb on + strand at 2374.084 kb on + strand at 2374.092 kb on - strand at 2374.105 kb on + strand at 2374.114 kb on - strand at 2374.260 kb on + strand, within Synpcc7942_2306 at 2374.283 kb on + strand, within Synpcc7942_2306 at 2374.293 kb on + strand, within Synpcc7942_2306 at 2374.301 kb on + strand, within Synpcc7942_2306 at 2374.331 kb on - strand at 2374.331 kb on - strand, within Synpcc7942_2306 at 2374.338 kb on - strand, within Synpcc7942_2306 at 2374.338 kb on - strand, within Synpcc7942_2306 at 2374.906 kb on + strand, within Synpcc7942_2307 at 2374.935 kb on - strand, within Synpcc7942_2307 at 2375.437 kb on + strand, within Synpcc7942_2307 at 2375.505 kb on - strand, within Synpcc7942_2307 at 2375.586 kb on - strand, within Synpcc7942_2307 at 2375.603 kb on + strand, within Synpcc7942_2307 at 2375.611 kb on - strand, within Synpcc7942_2307 at 2375.715 kb on + strand at 2375.853 kb on - strand at 2375.855 kb on + strand at 2375.855 kb on + strand at 2375.863 kb on - strand at 2375.921 kb on - strand at 2375.966 kb on - strand at 2375.994 kb on - strand, within gcvT at 2376.013 kb on + strand, within gcvT at 2376.128 kb on + strand at 2376.129 kb on + strand, within gcvT at 2376.129 kb on + strand, within gcvT at 2376.129 kb on + strand, within gcvT at 2376.129 kb on + strand, within gcvT at 2376.137 kb on - strand, within gcvT at 2376.141 kb on + strand, within gcvT at 2376.174 kb on - strand, within gcvT at 2376.208 kb on + strand, within gcvT at 2376.275 kb on + strand, within gcvT at 2376.275 kb on + strand, within gcvT at 2376.299 kb on + strand, within gcvT at 2376.305 kb on - strand, within gcvT at 2376.306 kb on - strand, within gcvT at 2376.323 kb on - strand, within gcvT at 2376.373 kb on - strand, within gcvT at 2376.390 kb on + strand, within gcvT at 2376.399 kb on + strand, within gcvT at 2376.408 kb on - strand, within gcvT at 2376.416 kb on + strand, within gcvT at 2376.468 kb on + strand, within gcvT at 2376.519 kb on - strand, within gcvT at 2376.522 kb on - strand, within gcvT at 2376.540 kb on + strand, within gcvT at 2376.545 kb on + strand, within gcvT at 2376.586 kb on + strand, within gcvT at 2376.604 kb on + strand, within gcvT at 2376.604 kb on - strand at 2376.612 kb on - strand, within gcvT at 2376.617 kb on + strand, within gcvT at 2376.623 kb on + strand, within gcvT at 2376.623 kb on + strand, within gcvT at 2376.632 kb on + strand, within gcvT at 2376.715 kb on - strand, within gcvT
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in Fresh BG11R in Flasks with Spin rep 1 remove 2,373,869 - nblR Synpcc7942_2305 0.81 +0.9 2,373,973 + -0.1 2,374,025 + -2.1 2,374,038 - -0.3 2,374,084 + -3.9 2,374,084 + -1.5 2,374,092 - -0.8 2,374,105 + -2.9 2,374,114 - +0.9 2,374,260 + Synpcc7942_2306 0.21 +1.9 2,374,283 + Synpcc7942_2306 0.25 +1.5 2,374,293 + Synpcc7942_2306 0.27 -2.8 2,374,301 + Synpcc7942_2306 0.28 +0.2 2,374,331 - +0.9 2,374,331 - Synpcc7942_2306 0.34 -2.5 2,374,338 - Synpcc7942_2306 0.35 +0.9 2,374,338 - Synpcc7942_2306 0.35 -0.6 2,374,906 + Synpcc7942_2307 0.18 -0.6 2,374,935 - Synpcc7942_2307 0.21 -0.1 2,375,437 + Synpcc7942_2307 0.70 -0.1 2,375,505 - Synpcc7942_2307 0.77 +0.9 2,375,586 - Synpcc7942_2307 0.85 -2.1 2,375,603 + Synpcc7942_2307 0.87 -3.1 2,375,611 - Synpcc7942_2307 0.88 -2.9 2,375,715 + -1.3 2,375,853 - -0.5 2,375,855 + +0.2 2,375,855 + +2.5 2,375,863 - -1.8 2,375,921 - -1.9 2,375,966 - -0.6 2,375,994 - gcvT Synpcc7942_2308 0.12 -1.4 2,376,013 + gcvT Synpcc7942_2308 0.13 -0.4 2,376,128 + +0.9 2,376,129 + gcvT Synpcc7942_2308 0.24 -1.0 2,376,129 + gcvT Synpcc7942_2308 0.24 -0.1 2,376,129 + gcvT Synpcc7942_2308 0.24 -1.5 2,376,129 + gcvT Synpcc7942_2308 0.24 +0.9 2,376,137 - gcvT Synpcc7942_2308 0.24 -0.1 2,376,141 + gcvT Synpcc7942_2308 0.25 -1.4 2,376,174 - gcvT Synpcc7942_2308 0.28 +0.9 2,376,208 + gcvT Synpcc7942_2308 0.31 +0.9 2,376,275 + gcvT Synpcc7942_2308 0.37 -0.1 2,376,275 + gcvT Synpcc7942_2308 0.37 -1.4 2,376,299 + gcvT Synpcc7942_2308 0.39 -0.6 2,376,305 - gcvT Synpcc7942_2308 0.39 -1.1 2,376,306 - gcvT Synpcc7942_2308 0.39 +0.9 2,376,323 - gcvT Synpcc7942_2308 0.41 +1.9 2,376,373 - gcvT Synpcc7942_2308 0.45 +0.9 2,376,390 + gcvT Synpcc7942_2308 0.47 +1.4 2,376,399 + gcvT Synpcc7942_2308 0.48 +2.5 2,376,408 - gcvT Synpcc7942_2308 0.49 -1.6 2,376,416 + gcvT Synpcc7942_2308 0.49 -2.1 2,376,468 + gcvT Synpcc7942_2308 0.54 +0.9 2,376,519 - gcvT Synpcc7942_2308 0.58 +0.9 2,376,522 - gcvT Synpcc7942_2308 0.59 +0.9 2,376,540 + gcvT Synpcc7942_2308 0.60 -1.8 2,376,545 + gcvT Synpcc7942_2308 0.61 -1.2 2,376,586 + gcvT Synpcc7942_2308 0.64 +0.9 2,376,604 + gcvT Synpcc7942_2308 0.66 +1.4 2,376,604 - -0.1 2,376,612 - gcvT Synpcc7942_2308 0.67 -0.1 2,376,617 + gcvT Synpcc7942_2308 0.67 +3.2 2,376,623 + gcvT Synpcc7942_2308 0.68 -1.1 2,376,623 + gcvT Synpcc7942_2308 0.68 -0.6 2,376,632 + gcvT Synpcc7942_2308 0.69 +0.9 2,376,715 - gcvT Synpcc7942_2308 0.76 -0.5
Or see this region's nucleotide sequence