Experiment: Biofilm in Fresh BG11R in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2014 and ycf66 are separated by 8 nucleotides ycf66 and psbX are separated by 117 nucleotides psbX and Synpcc7942_2017 are separated by 154 nucleotides Synpcc7942_2017 and tRNA-Thr are separated by 81 nucleotides tRNA-Thr and Synpcc7942_2018 are separated by 60 nucleotides Synpcc7942_2018 and Synpcc7942_2019 are separated by 63 nucleotides
Synpcc7942_2014: Synpcc7942_2014 - hypothetical protein, at 2,083,250 to 2,083,843
_2014
Synpcc7942_2015: ycf66 - conserved hypothetical protein YCF66, at 2,083,852 to 2,084,685
ycf66
Synpcc7942_2016: psbX - photosystem II PsbX protein, at 2,084,803 to 2,084,922
psbX
Synpcc7942_2017: Synpcc7942_2017 - hypothetical protein, at 2,085,077 to 2,085,370
_2017
Synpcc7942_R0003: tRNA-Thr - tRNA-Thr, at 2,085,452 to 2,085,523
tRNA-Thr
Synpcc7942_2018: Synpcc7942_2018 - hypothetical protein, at 2,085,584 to 2,085,829
_2018
Synpcc7942_2019: Synpcc7942_2019 - hypothetical protein, at 2,085,893 to 2,086,762
_2019
Position (kb)
2084
2085 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2083.840 kb on + strand at 2083.840 kb on + strand at 2083.840 kb on + strand at 2083.840 kb on + strand at 2083.848 kb on - strand at 2084.714 kb on + strand at 2084.737 kb on + strand at 2084.740 kb on + strand at 2084.741 kb on - strand at 2084.744 kb on + strand at 2084.750 kb on + strand at 2084.760 kb on + strand at 2084.781 kb on - strand at 2084.791 kb on - strand at 2084.851 kb on + strand, within psbX at 2084.868 kb on + strand, within psbX at 2084.878 kb on + strand, within psbX at 2084.886 kb on - strand, within psbX at 2084.886 kb on - strand, within psbX at 2084.949 kb on - strand at 2084.964 kb on + strand at 2084.972 kb on - strand at 2084.972 kb on - strand at 2085.002 kb on + strand at 2085.010 kb on - strand at 2085.014 kb on + strand at 2085.046 kb on + strand at 2085.053 kb on - strand at 2085.413 kb on - strand at 2085.564 kb on - strand at 2085.616 kb on + strand, within Synpcc7942_2018 at 2085.630 kb on - strand, within Synpcc7942_2018 at 2085.741 kb on + strand, within Synpcc7942_2018 at 2085.751 kb on + strand, within Synpcc7942_2018 at 2085.784 kb on - strand, within Synpcc7942_2018 at 2085.825 kb on - strand at 2085.831 kb on + strand at 2085.839 kb on - strand at 2085.852 kb on - strand at 2085.883 kb on + strand at 2085.885 kb on - strand at 2085.899 kb on - strand at 2085.907 kb on + strand at 2085.913 kb on - strand at 2085.913 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in Fresh BG11R in Flasks with Spin rep 1 remove 2,083,840 + -1.2 2,083,840 + -2.4 2,083,840 + -2.6 2,083,840 + -0.1 2,083,848 - -1.4 2,084,714 + +0.9 2,084,737 + -0.7 2,084,740 + -3.2 2,084,741 - -1.6 2,084,744 + +1.5 2,084,750 + +1.7 2,084,760 + +2.9 2,084,781 - +0.0 2,084,791 - +2.2 2,084,851 + psbX Synpcc7942_2016 0.40 -1.8 2,084,868 + psbX Synpcc7942_2016 0.54 -0.8 2,084,878 + psbX Synpcc7942_2016 0.62 -1.2 2,084,886 - psbX Synpcc7942_2016 0.69 -0.6 2,084,886 - psbX Synpcc7942_2016 0.69 -3.1 2,084,949 - +0.7 2,084,964 + +0.4 2,084,972 - +2.1 2,084,972 - -2.4 2,085,002 + -0.9 2,085,010 - -0.7 2,085,014 + -0.8 2,085,046 + -1.6 2,085,053 - -0.1 2,085,413 - +0.9 2,085,564 - -0.6 2,085,616 + Synpcc7942_2018 0.13 -0.8 2,085,630 - Synpcc7942_2018 0.19 -0.8 2,085,741 + Synpcc7942_2018 0.64 -1.1 2,085,751 + Synpcc7942_2018 0.68 -1.3 2,085,784 - Synpcc7942_2018 0.81 -0.4 2,085,825 - +1.7 2,085,831 + -1.0 2,085,839 - +1.0 2,085,852 - -0.3 2,085,883 + +0.4 2,085,885 - -0.9 2,085,899 - +0.9 2,085,907 + -1.0 2,085,913 - -3.2 2,085,913 - +0.9
Or see this region's nucleotide sequence