Experiment: Biofilm in Fresh BG11R in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ndhH and Synpcc7942_1744 are separated by 146 nucleotides Synpcc7942_1744 and Synpcc7942_1745 are separated by 14 nucleotides Synpcc7942_1745 and rpoD2 are separated by 206 nucleotides
Synpcc7942_1743: ndhH - NAD(P)H-quinone oxidoreductase subunit H, at 1,814,092 to 1,815,276
ndhH
Synpcc7942_1744: Synpcc7942_1744 - hypothetical protein, at 1,815,423 to 1,815,833
_1744
Synpcc7942_1745: Synpcc7942_1745 - nitrite transporter, HPP family (from data) , at 1,815,848 to 1,816,393
_1745
Synpcc7942_1746: rpoD2 - group2 RNA polymerase sigma factor SigB, at 1,816,600 to 1,817,562
rpoD2
Position (kb)
1815
1816
1817 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1815.321 kb on - strand at 1815.321 kb on - strand at 1815.328 kb on + strand at 1815.328 kb on + strand at 1815.336 kb on - strand at 1815.341 kb on - strand at 1815.349 kb on - strand at 1815.370 kb on - strand at 1815.413 kb on + strand at 1815.413 kb on + strand at 1815.413 kb on + strand at 1815.413 kb on + strand at 1815.413 kb on + strand at 1815.413 kb on + strand at 1815.413 kb on + strand at 1815.421 kb on - strand at 1815.421 kb on - strand at 1815.421 kb on - strand at 1815.421 kb on - strand at 1815.421 kb on - strand at 1815.421 kb on - strand at 1815.847 kb on + strand at 1815.857 kb on + strand at 1815.864 kb on + strand at 1815.864 kb on + strand at 1815.865 kb on - strand at 1815.865 kb on - strand at 1815.867 kb on - strand at 1815.867 kb on - strand at 1815.872 kb on - strand at 1815.900 kb on + strand at 1815.915 kb on + strand, within Synpcc7942_1745 at 1815.928 kb on + strand, within Synpcc7942_1745 at 1815.931 kb on - strand, within Synpcc7942_1745 at 1815.936 kb on - strand, within Synpcc7942_1745 at 1815.946 kb on - strand, within Synpcc7942_1745 at 1815.946 kb on - strand, within Synpcc7942_1745 at 1815.957 kb on + strand, within Synpcc7942_1745 at 1816.005 kb on - strand, within Synpcc7942_1745 at 1816.015 kb on + strand, within Synpcc7942_1745 at 1816.018 kb on + strand, within Synpcc7942_1745 at 1816.066 kb on + strand, within Synpcc7942_1745 at 1816.074 kb on + strand, within Synpcc7942_1745 at 1816.089 kb on + strand, within Synpcc7942_1745 at 1816.089 kb on - strand, within Synpcc7942_1745 at 1816.089 kb on - strand, within Synpcc7942_1745 at 1816.123 kb on - strand, within Synpcc7942_1745 at 1816.160 kb on + strand, within Synpcc7942_1745 at 1816.193 kb on + strand, within Synpcc7942_1745 at 1816.195 kb on - strand, within Synpcc7942_1745 at 1816.216 kb on - strand, within Synpcc7942_1745 at 1816.311 kb on + strand, within Synpcc7942_1745 at 1816.321 kb on + strand, within Synpcc7942_1745 at 1816.354 kb on - strand at 1816.436 kb on - strand at 1816.526 kb on + strand at 1816.534 kb on - strand at 1816.573 kb on + strand at 1816.599 kb on - strand at 1816.656 kb on - strand at 1816.735 kb on - strand, within rpoD2 at 1816.745 kb on + strand, within rpoD2 at 1816.745 kb on - strand, within rpoD2 at 1816.841 kb on - strand, within rpoD2 at 1816.925 kb on - strand, within rpoD2 at 1816.969 kb on + strand, within rpoD2 at 1817.113 kb on + strand, within rpoD2 at 1817.113 kb on + strand, within rpoD2 at 1817.113 kb on + strand, within rpoD2 at 1817.113 kb on + strand, within rpoD2 at 1817.113 kb on + strand, within rpoD2 at 1817.121 kb on - strand, within rpoD2 at 1817.121 kb on - strand, within rpoD2 at 1817.121 kb on - strand, within rpoD2 at 1817.121 kb on - strand, within rpoD2 at 1817.148 kb on - strand, within rpoD2 at 1817.163 kb on - strand, within rpoD2 at 1817.170 kb on - strand, within rpoD2 at 1817.252 kb on - strand, within rpoD2 at 1817.322 kb on + strand, within rpoD2 at 1817.373 kb on + strand, within rpoD2 at 1817.388 kb on + strand, within rpoD2
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in Fresh BG11R in Flasks with Spin rep 1 remove 1,815,321 - -0.2 1,815,321 - +0.0 1,815,328 + +2.3 1,815,328 + -1.6 1,815,336 - +0.9 1,815,341 - +2.1 1,815,349 - -1.4 1,815,370 - +1.7 1,815,413 + -3.3 1,815,413 + +0.0 1,815,413 + -2.1 1,815,413 + -0.3 1,815,413 + -0.2 1,815,413 + -0.8 1,815,413 + +3.9 1,815,421 - -2.5 1,815,421 - +3.3 1,815,421 - -0.8 1,815,421 - -1.7 1,815,421 - -0.1 1,815,421 - +1.5 1,815,847 + -0.1 1,815,857 + +0.9 1,815,864 + -1.9 1,815,864 + -1.0 1,815,865 - -2.3 1,815,865 - -0.7 1,815,867 - -1.1 1,815,867 - -2.0 1,815,872 - -0.7 1,815,900 + -0.4 1,815,915 + Synpcc7942_1745 0.12 -1.7 1,815,928 + Synpcc7942_1745 0.15 -0.9 1,815,931 - Synpcc7942_1745 0.15 +0.9 1,815,936 - Synpcc7942_1745 0.16 -0.7 1,815,946 - Synpcc7942_1745 0.18 -0.4 1,815,946 - Synpcc7942_1745 0.18 +0.9 1,815,957 + Synpcc7942_1745 0.20 -0.1 1,816,005 - Synpcc7942_1745 0.29 -1.5 1,816,015 + Synpcc7942_1745 0.31 -0.1 1,816,018 + Synpcc7942_1745 0.31 -1.1 1,816,066 + Synpcc7942_1745 0.40 -2.0 1,816,074 + Synpcc7942_1745 0.41 +0.9 1,816,089 + Synpcc7942_1745 0.44 -0.9 1,816,089 - Synpcc7942_1745 0.44 +0.9 1,816,089 - Synpcc7942_1745 0.44 -2.3 1,816,123 - Synpcc7942_1745 0.50 +0.9 1,816,160 + Synpcc7942_1745 0.57 -4.9 1,816,193 + Synpcc7942_1745 0.63 -0.9 1,816,195 - Synpcc7942_1745 0.64 +0.2 1,816,216 - Synpcc7942_1745 0.67 -1.8 1,816,311 + Synpcc7942_1745 0.85 -0.1 1,816,321 + Synpcc7942_1745 0.87 -1.6 1,816,354 - -0.8 1,816,436 - -2.0 1,816,526 + +1.5 1,816,534 - +0.5 1,816,573 + -1.0 1,816,599 - -1.1 1,816,656 - -0.0 1,816,735 - rpoD2 Synpcc7942_1746 0.14 +0.9 1,816,745 + rpoD2 Synpcc7942_1746 0.15 -1.0 1,816,745 - rpoD2 Synpcc7942_1746 0.15 +0.9 1,816,841 - rpoD2 Synpcc7942_1746 0.25 -0.5 1,816,925 - rpoD2 Synpcc7942_1746 0.34 -2.5 1,816,969 + rpoD2 Synpcc7942_1746 0.38 -2.1 1,817,113 + rpoD2 Synpcc7942_1746 0.53 -0.7 1,817,113 + rpoD2 Synpcc7942_1746 0.53 -2.8 1,817,113 + rpoD2 Synpcc7942_1746 0.53 +0.9 1,817,113 + rpoD2 Synpcc7942_1746 0.53 +0.5 1,817,113 + rpoD2 Synpcc7942_1746 0.53 +0.9 1,817,121 - rpoD2 Synpcc7942_1746 0.54 -0.7 1,817,121 - rpoD2 Synpcc7942_1746 0.54 -0.1 1,817,121 - rpoD2 Synpcc7942_1746 0.54 +0.7 1,817,121 - rpoD2 Synpcc7942_1746 0.54 +2.5 1,817,148 - rpoD2 Synpcc7942_1746 0.57 +0.9 1,817,163 - rpoD2 Synpcc7942_1746 0.58 -0.6 1,817,170 - rpoD2 Synpcc7942_1746 0.59 +2.5 1,817,252 - rpoD2 Synpcc7942_1746 0.68 -0.0 1,817,322 + rpoD2 Synpcc7942_1746 0.75 -0.6 1,817,373 + rpoD2 Synpcc7942_1746 0.80 +0.5 1,817,388 + rpoD2 Synpcc7942_1746 0.82 -0.4
Or see this region's nucleotide sequence