Experiment: Biofilm in Fresh BG11R in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glsF and Synpcc7942_0891 are separated by 377 nucleotides Synpcc7942_0891 and cofG are separated by 73 nucleotides cofG and psbAIII are separated by 81 nucleotides
Synpcc7942_0890: glsF - glutamate synthase (ferredoxin), at 896,748 to 901,358
glsF
Synpcc7942_0891: Synpcc7942_0891 - hypothetical protein, at 901,736 to 902,035
_0891
Synpcc7942_0892: cofG - FO synthase subunit 1, at 902,109 to 903,119
cofG
Synpcc7942_0893: psbAIII - photosystem q(b) protein, at 903,201 to 904,283
psbAIII
Position (kb) 902
903
904 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 901.309 kb on + strand at 901.329 kb on - strand at 901.329 kb on - strand at 901.353 kb on + strand at 901.353 kb on + strand at 901.361 kb on - strand at 901.387 kb on - strand at 901.410 kb on + strand at 901.433 kb on + strand at 901.441 kb on - strand at 901.443 kb on + strand at 901.467 kb on + strand at 901.492 kb on + strand at 901.642 kb on - strand at 901.651 kb on - strand at 901.671 kb on - strand at 901.687 kb on + strand at 901.776 kb on + strand, within Synpcc7942_0891 at 901.776 kb on + strand, within Synpcc7942_0891 at 901.776 kb on + strand, within Synpcc7942_0891 at 901.781 kb on + strand, within Synpcc7942_0891 at 901.786 kb on + strand, within Synpcc7942_0891 at 901.907 kb on - strand, within Synpcc7942_0891 at 901.973 kb on + strand, within Synpcc7942_0891 at 901.991 kb on + strand, within Synpcc7942_0891 at 901.991 kb on + strand, within Synpcc7942_0891 at 901.999 kb on - strand, within Synpcc7942_0891 at 902.015 kb on - strand at 902.024 kb on - strand at 902.037 kb on - strand at 902.037 kb on - strand at 902.064 kb on + strand at 902.078 kb on + strand at 902.116 kb on + strand at 902.116 kb on + strand at 902.124 kb on - strand at 902.137 kb on - strand at 902.144 kb on + strand at 902.182 kb on - strand at 902.192 kb on + strand at 902.200 kb on - strand at 902.223 kb on + strand, within cofG at 902.229 kb on + strand, within cofG at 902.248 kb on + strand, within cofG at 902.298 kb on + strand, within cofG at 902.303 kb on + strand, within cofG at 902.311 kb on - strand, within cofG at 902.311 kb on - strand, within cofG at 902.333 kb on + strand, within cofG at 902.341 kb on - strand, within cofG at 902.343 kb on - strand, within cofG at 902.380 kb on - strand, within cofG at 902.403 kb on + strand, within cofG at 902.411 kb on - strand, within cofG at 902.463 kb on + strand, within cofG at 902.463 kb on + strand, within cofG at 902.473 kb on - strand, within cofG at 902.502 kb on + strand, within cofG at 902.550 kb on - strand, within cofG at 902.561 kb on + strand, within cofG at 902.589 kb on - strand, within cofG at 902.592 kb on + strand, within cofG at 902.611 kb on - strand, within cofG at 902.618 kb on - strand, within cofG at 902.629 kb on + strand, within cofG at 902.629 kb on + strand, within cofG at 902.639 kb on + strand, within cofG at 902.639 kb on + strand, within cofG at 902.647 kb on - strand, within cofG at 902.647 kb on - strand, within cofG at 902.647 kb on - strand, within cofG at 902.676 kb on - strand, within cofG at 902.679 kb on - strand, within cofG at 902.686 kb on - strand, within cofG at 902.688 kb on + strand, within cofG at 902.715 kb on + strand, within cofG at 902.734 kb on + strand, within cofG at 902.739 kb on - strand, within cofG at 902.750 kb on - strand, within cofG at 902.776 kb on - strand, within cofG at 902.778 kb on + strand, within cofG at 902.778 kb on + strand, within cofG at 902.786 kb on - strand, within cofG at 902.786 kb on - strand, within cofG at 902.791 kb on + strand at 902.791 kb on + strand, within cofG at 902.799 kb on - strand, within cofG at 902.817 kb on + strand, within cofG at 902.825 kb on - strand, within cofG at 902.825 kb on - strand, within cofG at 902.861 kb on - strand, within cofG at 902.933 kb on - strand, within cofG at 902.964 kb on + strand, within cofG at 902.965 kb on - strand, within cofG at 902.972 kb on - strand, within cofG at 903.010 kb on + strand, within cofG at 903.035 kb on - strand at 903.053 kb on - strand at 903.054 kb on - strand at 903.074 kb on - strand at 903.078 kb on + strand at 903.078 kb on + strand at 903.229 kb on - strand at 903.311 kb on - strand, within psbAIII
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in Fresh BG11R in Flasks with Spin rep 1 remove 901,309 + +3.0 901,329 - +1.0 901,329 - -1.2 901,353 + +1.5 901,353 + -0.8 901,361 - -1.0 901,387 - -0.5 901,410 + -0.6 901,433 + -1.0 901,441 - +1.7 901,443 + -0.1 901,467 + -2.5 901,492 + -1.3 901,642 - -0.3 901,651 - +1.8 901,671 - -1.9 901,687 + -2.7 901,776 + Synpcc7942_0891 0.13 -0.3 901,776 + Synpcc7942_0891 0.13 -2.5 901,776 + Synpcc7942_0891 0.13 -1.3 901,781 + Synpcc7942_0891 0.15 +2.0 901,786 + Synpcc7942_0891 0.17 -0.9 901,907 - Synpcc7942_0891 0.57 +1.0 901,973 + Synpcc7942_0891 0.79 -1.7 901,991 + Synpcc7942_0891 0.85 +1.4 901,991 + Synpcc7942_0891 0.85 +2.4 901,999 - Synpcc7942_0891 0.88 -2.3 902,015 - -1.0 902,024 - +1.7 902,037 - -0.5 902,037 - -0.0 902,064 + +2.2 902,078 + +1.0 902,116 + -0.6 902,116 + -0.0 902,124 - -1.4 902,137 - +0.4 902,144 + -0.0 902,182 - -3.4 902,192 + -1.6 902,200 - +1.0 902,223 + cofG Synpcc7942_0892 0.11 -0.4 902,229 + cofG Synpcc7942_0892 0.12 +1.4 902,248 + cofG Synpcc7942_0892 0.14 -3.4 902,298 + cofG Synpcc7942_0892 0.19 +2.4 902,303 + cofG Synpcc7942_0892 0.19 +1.0 902,311 - cofG Synpcc7942_0892 0.20 -2.9 902,311 - cofG Synpcc7942_0892 0.20 -3.2 902,333 + cofG Synpcc7942_0892 0.22 +1.2 902,341 - cofG Synpcc7942_0892 0.23 -1.7 902,343 - cofG Synpcc7942_0892 0.23 -0.5 902,380 - cofG Synpcc7942_0892 0.27 -1.2 902,403 + cofG Synpcc7942_0892 0.29 +1.0 902,411 - cofG Synpcc7942_0892 0.30 -0.0 902,463 + cofG Synpcc7942_0892 0.35 -0.6 902,463 + cofG Synpcc7942_0892 0.35 +2.0 902,473 - cofG Synpcc7942_0892 0.36 -1.1 902,502 + cofG Synpcc7942_0892 0.39 -1.0 902,550 - cofG Synpcc7942_0892 0.44 +1.0 902,561 + cofG Synpcc7942_0892 0.45 -2.7 902,589 - cofG Synpcc7942_0892 0.47 -2.4 902,592 + cofG Synpcc7942_0892 0.48 -0.3 902,611 - cofG Synpcc7942_0892 0.50 -0.7 902,618 - cofG Synpcc7942_0892 0.50 +0.2 902,629 + cofG Synpcc7942_0892 0.51 +0.5 902,629 + cofG Synpcc7942_0892 0.51 -2.6 902,639 + cofG Synpcc7942_0892 0.52 -0.5 902,639 + cofG Synpcc7942_0892 0.52 -1.5 902,647 - cofG Synpcc7942_0892 0.53 -0.0 902,647 - cofG Synpcc7942_0892 0.53 -1.7 902,647 - cofG Synpcc7942_0892 0.53 +0.4 902,676 - cofG Synpcc7942_0892 0.56 -2.0 902,679 - cofG Synpcc7942_0892 0.56 -2.1 902,686 - cofG Synpcc7942_0892 0.57 -2.9 902,688 + cofG Synpcc7942_0892 0.57 -2.0 902,715 + cofG Synpcc7942_0892 0.60 -1.4 902,734 + cofG Synpcc7942_0892 0.62 -1.3 902,739 - cofG Synpcc7942_0892 0.62 -2.7 902,750 - cofG Synpcc7942_0892 0.63 -2.6 902,776 - cofG Synpcc7942_0892 0.66 -0.2 902,778 + cofG Synpcc7942_0892 0.66 -1.4 902,778 + cofG Synpcc7942_0892 0.66 -0.4 902,786 - cofG Synpcc7942_0892 0.67 +0.4 902,786 - cofG Synpcc7942_0892 0.67 -1.6 902,791 + -1.4 902,791 + cofG Synpcc7942_0892 0.67 -2.8 902,799 - cofG Synpcc7942_0892 0.68 -1.3 902,817 + cofG Synpcc7942_0892 0.70 -2.8 902,825 - cofG Synpcc7942_0892 0.71 -1.7 902,825 - cofG Synpcc7942_0892 0.71 -1.9 902,861 - cofG Synpcc7942_0892 0.74 -0.4 902,933 - cofG Synpcc7942_0892 0.82 -2.2 902,964 + cofG Synpcc7942_0892 0.85 -0.8 902,965 - cofG Synpcc7942_0892 0.85 +0.4 902,972 - cofG Synpcc7942_0892 0.85 -2.8 903,010 + cofG Synpcc7942_0892 0.89 +0.7 903,035 - -1.6 903,053 - -0.1 903,054 - -1.0 903,074 - -0.2 903,078 + -0.4 903,078 + -0.0 903,229 - -0.2 903,311 - psbAIII Synpcc7942_0893 0.10 +3.1
Or see this region's nucleotide sequence