Experiment: Biofilm in Fresh BG11R in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0782 and hisG are separated by 32 nucleotides hisG and Synpcc7942_0784 are separated by 285 nucleotides Synpcc7942_0784 and ponA overlap by 68 nucleotides
Synpcc7942_0782: Synpcc7942_0782 - ATPase, at 776,760 to 778,556
_0782
Synpcc7942_0783: hisG - ATP phosphoribosyltransferase catalytic subunit, at 778,589 to 779,245
hisG
Synpcc7942_0784: Synpcc7942_0784 - hypothetical protein, at 779,531 to 779,848
_0784
Synpcc7942_0785: ponA - Penicillin-binding protein 1A, at 779,781 to 781,919
ponA
Position (kb) 779
780 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 778.545 kb on - strand at 778.547 kb on - strand at 778.573 kb on - strand at 778.575 kb on + strand at 778.575 kb on + strand at 778.580 kb on + strand at 778.583 kb on - strand at 778.583 kb on - strand at 778.610 kb on - strand at 778.612 kb on + strand at 778.622 kb on - strand at 779.280 kb on - strand at 779.284 kb on + strand at 779.297 kb on - strand at 779.307 kb on - strand at 779.312 kb on - strand at 779.314 kb on + strand at 779.322 kb on - strand at 779.322 kb on - strand at 779.322 kb on - strand at 779.324 kb on + strand at 779.326 kb on + strand at 779.348 kb on - strand at 779.353 kb on - strand at 779.357 kb on + strand at 779.369 kb on + strand at 779.398 kb on - strand at 779.447 kb on + strand at 779.455 kb on - strand at 779.491 kb on + strand at 779.497 kb on - strand at 779.499 kb on - strand at 779.515 kb on - strand at 779.531 kb on + strand at 779.539 kb on - strand at 779.555 kb on + strand at 779.563 kb on - strand, within Synpcc7942_0784 at 779.581 kb on + strand, within Synpcc7942_0784 at 779.604 kb on + strand, within Synpcc7942_0784 at 779.612 kb on + strand, within Synpcc7942_0784 at 779.612 kb on + strand, within Synpcc7942_0784 at 779.619 kb on - strand, within Synpcc7942_0784 at 779.620 kb on - strand, within Synpcc7942_0784 at 779.653 kb on + strand at 779.742 kb on + strand, within Synpcc7942_0784 at 779.745 kb on - strand, within Synpcc7942_0784 at 779.750 kb on - strand, within Synpcc7942_0784 at 779.756 kb on - strand, within Synpcc7942_0784 at 779.791 kb on + strand at 779.822 kb on - strand at 779.822 kb on - strand at 779.843 kb on + strand at 779.850 kb on - strand at 779.895 kb on - strand at 779.896 kb on - strand at 779.923 kb on - strand at 779.944 kb on + strand at 779.964 kb on - strand at 779.968 kb on - strand at 779.982 kb on + strand at 780.003 kb on - strand, within ponA at 780.015 kb on + strand, within ponA at 780.020 kb on + strand, within ponA at 780.020 kb on + strand at 780.025 kb on + strand, within ponA at 780.028 kb on - strand, within ponA at 780.028 kb on - strand, within ponA at 780.028 kb on - strand, within ponA at 780.038 kb on - strand, within ponA at 780.051 kb on - strand, within ponA at 780.061 kb on + strand, within ponA at 780.061 kb on - strand, within ponA at 780.065 kb on + strand, within ponA at 780.071 kb on + strand, within ponA at 780.073 kb on - strand, within ponA at 780.075 kb on + strand, within ponA at 780.076 kb on + strand, within ponA at 780.084 kb on + strand, within ponA at 780.096 kb on - strand, within ponA at 780.098 kb on - strand, within ponA at 780.099 kb on - strand, within ponA at 780.141 kb on - strand, within ponA at 780.146 kb on + strand, within ponA at 780.157 kb on - strand, within ponA at 780.177 kb on + strand, within ponA at 780.202 kb on + strand, within ponA at 780.210 kb on - strand, within ponA at 780.224 kb on + strand, within ponA at 780.237 kb on - strand, within ponA at 780.242 kb on + strand at 780.263 kb on + strand, within ponA at 780.271 kb on - strand, within ponA at 780.272 kb on - strand, within ponA at 780.281 kb on - strand, within ponA at 780.288 kb on + strand, within ponA at 780.288 kb on + strand, within ponA at 780.296 kb on - strand, within ponA at 780.296 kb on - strand, within ponA at 780.321 kb on - strand, within ponA at 780.335 kb on - strand, within ponA at 780.357 kb on + strand, within ponA at 780.357 kb on + strand, within ponA at 780.357 kb on + strand, within ponA at 780.365 kb on - strand, within ponA at 780.365 kb on - strand, within ponA at 780.365 kb on - strand, within ponA at 780.365 kb on - strand, within ponA at 780.365 kb on - strand, within ponA at 780.367 kb on + strand, within ponA at 780.367 kb on + strand, within ponA at 780.367 kb on + strand, within ponA at 780.367 kb on + strand, within ponA at 780.367 kb on + strand, within ponA at 780.375 kb on - strand, within ponA at 780.375 kb on - strand, within ponA at 780.375 kb on - strand, within ponA at 780.375 kb on - strand, within ponA at 780.379 kb on + strand, within ponA at 780.385 kb on - strand, within ponA at 780.386 kb on - strand, within ponA at 780.386 kb on - strand, within ponA at 780.389 kb on + strand, within ponA at 780.389 kb on + strand, within ponA at 780.389 kb on + strand, within ponA at 780.389 kb on + strand, within ponA at 780.397 kb on - strand, within ponA at 780.397 kb on - strand, within ponA at 780.397 kb on - strand, within ponA at 780.397 kb on - strand, within ponA at 780.397 kb on - strand, within ponA at 780.397 kb on - strand, within ponA at 780.397 kb on - strand, within ponA at 780.397 kb on - strand, within ponA at 780.407 kb on - strand, within ponA at 780.412 kb on + strand, within ponA at 780.435 kb on - strand, within ponA at 780.443 kb on + strand, within ponA at 780.445 kb on - strand, within ponA at 780.466 kb on - strand, within ponA at 780.471 kb on + strand, within ponA at 780.478 kb on + strand, within ponA at 780.515 kb on + strand, within ponA at 780.515 kb on + strand, within ponA at 780.567 kb on + strand, within ponA at 780.600 kb on + strand, within ponA at 780.601 kb on - strand, within ponA at 780.629 kb on + strand, within ponA at 780.629 kb on + strand, within ponA at 780.637 kb on - strand, within ponA at 780.647 kb on - strand, within ponA at 780.672 kb on + strand, within ponA at 780.672 kb on + strand, within ponA at 780.677 kb on + strand, within ponA at 780.680 kb on - strand, within ponA at 780.680 kb on - strand, within ponA at 780.685 kb on - strand, within ponA at 780.697 kb on - strand, within ponA at 780.732 kb on - strand, within ponA at 780.752 kb on - strand at 780.759 kb on + strand, within ponA at 780.759 kb on + strand, within ponA at 780.759 kb on + strand, within ponA at 780.759 kb on + strand, within ponA at 780.759 kb on + strand, within ponA at 780.767 kb on - strand, within ponA at 780.767 kb on - strand, within ponA at 780.767 kb on - strand, within ponA at 780.767 kb on - strand, within ponA at 780.767 kb on - strand, within ponA at 780.772 kb on - strand at 780.772 kb on - strand, within ponA at 780.772 kb on - strand, within ponA at 780.842 kb on + strand, within ponA
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in Fresh BG11R in Flasks with Spin rep 1 remove 778,545 - +0.9 778,547 - -1.3 778,573 - +2.7 778,575 + +1.1 778,575 + +0.7 778,580 + -2.3 778,583 - +1.9 778,583 - -0.5 778,610 - -2.6 778,612 + -0.2 778,622 - -0.1 779,280 - +0.9 779,284 + -1.9 779,297 - +0.5 779,307 - +0.2 779,312 - +0.6 779,314 + -1.7 779,322 - +0.1 779,322 - -0.8 779,322 - -0.1 779,324 + -0.5 779,326 + -0.7 779,348 - -2.7 779,353 - -2.1 779,357 + -0.1 779,369 + +0.9 779,398 - -1.4 779,447 + +0.6 779,455 - -0.7 779,491 + -1.2 779,497 - +1.5 779,499 - +1.6 779,515 - -1.8 779,531 + -1.2 779,539 - -0.3 779,555 + -0.6 779,563 - Synpcc7942_0784 0.10 -1.2 779,581 + Synpcc7942_0784 0.16 -1.5 779,604 + Synpcc7942_0784 0.23 -1.1 779,612 + Synpcc7942_0784 0.25 +0.4 779,612 + Synpcc7942_0784 0.25 -1.2 779,619 - Synpcc7942_0784 0.28 -3.4 779,620 - Synpcc7942_0784 0.28 -1.0 779,653 + +0.1 779,742 + Synpcc7942_0784 0.66 -0.6 779,745 - Synpcc7942_0784 0.67 +1.4 779,750 - Synpcc7942_0784 0.69 +0.9 779,756 - Synpcc7942_0784 0.71 -1.3 779,791 + -1.6 779,822 - -1.6 779,822 - -3.2 779,843 + +0.9 779,850 - -0.3 779,895 - -0.4 779,896 - -3.6 779,923 - -0.6 779,944 + -0.9 779,964 - +0.5 779,968 - -1.1 779,982 + -1.1 780,003 - ponA Synpcc7942_0785 0.10 +1.4 780,015 + ponA Synpcc7942_0785 0.11 -0.1 780,020 + ponA Synpcc7942_0785 0.11 +1.7 780,020 + -0.1 780,025 + ponA Synpcc7942_0785 0.11 -0.5 780,028 - ponA Synpcc7942_0785 0.12 +0.9 780,028 - ponA Synpcc7942_0785 0.12 +0.9 780,028 - ponA Synpcc7942_0785 0.12 -0.2 780,038 - ponA Synpcc7942_0785 0.12 +1.5 780,051 - ponA Synpcc7942_0785 0.13 -0.6 780,061 + ponA Synpcc7942_0785 0.13 -1.3 780,061 - ponA Synpcc7942_0785 0.13 -1.9 780,065 + ponA Synpcc7942_0785 0.13 -0.9 780,071 + ponA Synpcc7942_0785 0.14 -1.6 780,073 - ponA Synpcc7942_0785 0.14 +0.1 780,075 + ponA Synpcc7942_0785 0.14 +0.5 780,076 + ponA Synpcc7942_0785 0.14 -2.0 780,084 + ponA Synpcc7942_0785 0.14 -0.9 780,096 - ponA Synpcc7942_0785 0.15 -2.9 780,098 - ponA Synpcc7942_0785 0.15 +0.9 780,099 - ponA Synpcc7942_0785 0.15 -0.5 780,141 - ponA Synpcc7942_0785 0.17 -1.1 780,146 + ponA Synpcc7942_0785 0.17 +0.4 780,157 - ponA Synpcc7942_0785 0.18 -1.3 780,177 + ponA Synpcc7942_0785 0.19 -3.4 780,202 + ponA Synpcc7942_0785 0.20 +0.4 780,210 - ponA Synpcc7942_0785 0.20 -0.6 780,224 + ponA Synpcc7942_0785 0.21 +0.4 780,237 - ponA Synpcc7942_0785 0.21 -0.3 780,242 + -1.1 780,263 + ponA Synpcc7942_0785 0.23 -0.1 780,271 - ponA Synpcc7942_0785 0.23 -1.4 780,272 - ponA Synpcc7942_0785 0.23 -0.4 780,281 - ponA Synpcc7942_0785 0.23 +0.9 780,288 + ponA Synpcc7942_0785 0.24 -0.9 780,288 + ponA Synpcc7942_0785 0.24 -2.9 780,296 - ponA Synpcc7942_0785 0.24 +1.2 780,296 - ponA Synpcc7942_0785 0.24 +1.2 780,321 - ponA Synpcc7942_0785 0.25 +1.9 780,335 - ponA Synpcc7942_0785 0.26 -2.2 780,357 + ponA Synpcc7942_0785 0.27 -0.7 780,357 + ponA Synpcc7942_0785 0.27 +2.3 780,357 + ponA Synpcc7942_0785 0.27 -1.8 780,365 - ponA Synpcc7942_0785 0.27 -0.7 780,365 - ponA Synpcc7942_0785 0.27 -0.1 780,365 - ponA Synpcc7942_0785 0.27 -0.8 780,365 - ponA Synpcc7942_0785 0.27 -3.1 780,365 - ponA Synpcc7942_0785 0.27 +0.3 780,367 + ponA Synpcc7942_0785 0.27 +0.9 780,367 + ponA Synpcc7942_0785 0.27 -1.1 780,367 + ponA Synpcc7942_0785 0.27 -2.3 780,367 + ponA Synpcc7942_0785 0.27 -0.5 780,367 + ponA Synpcc7942_0785 0.27 +1.3 780,375 - ponA Synpcc7942_0785 0.28 +1.3 780,375 - ponA Synpcc7942_0785 0.28 -0.4 780,375 - ponA Synpcc7942_0785 0.28 +0.1 780,375 - ponA Synpcc7942_0785 0.28 +1.9 780,379 + ponA Synpcc7942_0785 0.28 -1.7 780,385 - ponA Synpcc7942_0785 0.28 +1.9 780,386 - ponA Synpcc7942_0785 0.28 -1.0 780,386 - ponA Synpcc7942_0785 0.28 +0.9 780,389 + ponA Synpcc7942_0785 0.28 +0.9 780,389 + ponA Synpcc7942_0785 0.28 -0.1 780,389 + ponA Synpcc7942_0785 0.28 +1.9 780,389 + ponA Synpcc7942_0785 0.28 -1.9 780,397 - ponA Synpcc7942_0785 0.29 +0.9 780,397 - ponA Synpcc7942_0785 0.29 -0.3 780,397 - ponA Synpcc7942_0785 0.29 +0.9 780,397 - ponA Synpcc7942_0785 0.29 +0.3 780,397 - ponA Synpcc7942_0785 0.29 -0.4 780,397 - ponA Synpcc7942_0785 0.29 -3.8 780,397 - ponA Synpcc7942_0785 0.29 -1.5 780,397 - ponA Synpcc7942_0785 0.29 -0.3 780,407 - ponA Synpcc7942_0785 0.29 -1.5 780,412 + ponA Synpcc7942_0785 0.29 -1.2 780,435 - ponA Synpcc7942_0785 0.31 -1.2 780,443 + ponA Synpcc7942_0785 0.31 +0.9 780,445 - ponA Synpcc7942_0785 0.31 -0.9 780,466 - ponA Synpcc7942_0785 0.32 -1.7 780,471 + ponA Synpcc7942_0785 0.32 -0.8 780,478 + ponA Synpcc7942_0785 0.33 +1.9 780,515 + ponA Synpcc7942_0785 0.34 -1.2 780,515 + ponA Synpcc7942_0785 0.34 -3.3 780,567 + ponA Synpcc7942_0785 0.37 -1.2 780,600 + ponA Synpcc7942_0785 0.38 -0.6 780,601 - ponA Synpcc7942_0785 0.38 -2.1 780,629 + ponA Synpcc7942_0785 0.40 -0.2 780,629 + ponA Synpcc7942_0785 0.40 +0.9 780,637 - ponA Synpcc7942_0785 0.40 -1.1 780,647 - ponA Synpcc7942_0785 0.40 -2.8 780,672 + ponA Synpcc7942_0785 0.42 -1.1 780,672 + ponA Synpcc7942_0785 0.42 -2.8 780,677 + ponA Synpcc7942_0785 0.42 +2.5 780,680 - ponA Synpcc7942_0785 0.42 +1.5 780,680 - ponA Synpcc7942_0785 0.42 +1.9 780,685 - ponA Synpcc7942_0785 0.42 +0.3 780,697 - ponA Synpcc7942_0785 0.43 -0.4 780,732 - ponA Synpcc7942_0785 0.44 -0.1 780,752 - -0.5 780,759 + ponA Synpcc7942_0785 0.46 +1.9 780,759 + ponA Synpcc7942_0785 0.46 -1.4 780,759 + ponA Synpcc7942_0785 0.46 -1.1 780,759 + ponA Synpcc7942_0785 0.46 -0.9 780,759 + ponA Synpcc7942_0785 0.46 +1.7 780,767 - ponA Synpcc7942_0785 0.46 -0.3 780,767 - ponA Synpcc7942_0785 0.46 +1.5 780,767 - ponA Synpcc7942_0785 0.46 -1.7 780,767 - ponA Synpcc7942_0785 0.46 -0.1 780,767 - ponA Synpcc7942_0785 0.46 -1.2 780,772 - -0.1 780,772 - ponA Synpcc7942_0785 0.46 +0.3 780,772 - ponA Synpcc7942_0785 0.46 -0.7 780,842 + ponA Synpcc7942_0785 0.50 +1.3
Or see this region's nucleotide sequence