Experiment: Biofilm in Fresh BG11R in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tgt and psbK are separated by 53 nucleotides psbK and Synpcc7942_0457 are separated by 215 nucleotides Synpcc7942_0457 and fghA are separated by 35 nucleotides fghA and Synpcc7942_0459 are separated by 5 nucleotides
Synpcc7942_0455: tgt - queuine tRNA-ribosyltransferase, at 444,001 to 445,155
tgt
Synpcc7942_0456: psbK - photosystem II reaction center protein PsbK precursor, at 445,209 to 445,346
psbK
Synpcc7942_0457: Synpcc7942_0457 - hypothetical protein, at 445,562 to 446,179
_0457
Synpcc7942_0458: fghA - Carboxylesterase, at 446,215 to 447,051
fghA
Synpcc7942_0459: Synpcc7942_0459 - glutathione-dependent formaldehyde dehydrogenase, at 447,057 to 448,166
_0459
Position (kb) 445
446
447 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 444.576 kb on - strand, within tgt at 444.595 kb on + strand, within tgt at 444.595 kb on + strand, within tgt at 444.595 kb on + strand, within tgt at 444.595 kb on + strand, within tgt at 444.603 kb on - strand, within tgt at 444.603 kb on - strand, within tgt at 444.603 kb on - strand, within tgt at 444.603 kb on - strand, within tgt at 444.738 kb on + strand, within tgt at 444.749 kb on + strand, within tgt at 444.749 kb on + strand, within tgt at 444.749 kb on + strand, within tgt at 444.757 kb on - strand, within tgt at 444.773 kb on + strand, within tgt at 444.796 kb on + strand, within tgt at 444.858 kb on + strand, within tgt at 444.858 kb on + strand, within tgt at 444.871 kb on - strand, within tgt at 444.902 kb on + strand, within tgt at 444.948 kb on + strand, within tgt at 445.014 kb on + strand, within tgt at 445.022 kb on - strand, within tgt at 445.092 kb on - strand at 445.116 kb on + strand at 445.129 kb on - strand at 445.129 kb on - strand at 445.148 kb on - strand at 445.208 kb on + strand at 445.218 kb on + strand at 445.293 kb on - strand, within psbK at 445.349 kb on - strand at 445.356 kb on - strand at 445.364 kb on - strand at 445.395 kb on + strand at 445.446 kb on - strand at 445.573 kb on - strand at 445.583 kb on + strand at 445.593 kb on + strand at 445.601 kb on - strand at 445.641 kb on + strand, within Synpcc7942_0457 at 445.670 kb on + strand, within Synpcc7942_0457 at 445.670 kb on + strand at 445.670 kb on + strand, within Synpcc7942_0457 at 445.670 kb on + strand, within Synpcc7942_0457 at 445.675 kb on + strand at 445.678 kb on - strand, within Synpcc7942_0457 at 445.678 kb on - strand, within Synpcc7942_0457 at 445.678 kb on - strand, within Synpcc7942_0457 at 445.678 kb on - strand, within Synpcc7942_0457 at 445.681 kb on + strand, within Synpcc7942_0457 at 445.686 kb on + strand, within Synpcc7942_0457 at 445.689 kb on - strand, within Synpcc7942_0457 at 445.691 kb on - strand, within Synpcc7942_0457 at 445.755 kb on + strand, within Synpcc7942_0457 at 445.763 kb on - strand, within Synpcc7942_0457 at 445.771 kb on + strand, within Synpcc7942_0457 at 445.783 kb on + strand, within Synpcc7942_0457 at 445.790 kb on + strand, within Synpcc7942_0457 at 445.798 kb on - strand, within Synpcc7942_0457 at 445.799 kb on - strand, within Synpcc7942_0457 at 445.806 kb on + strand, within Synpcc7942_0457 at 445.814 kb on - strand, within Synpcc7942_0457 at 445.832 kb on + strand, within Synpcc7942_0457 at 445.874 kb on - strand, within Synpcc7942_0457 at 445.897 kb on + strand, within Synpcc7942_0457 at 445.900 kb on - strand, within Synpcc7942_0457 at 445.933 kb on + strand, within Synpcc7942_0457 at 445.933 kb on + strand, within Synpcc7942_0457 at 445.933 kb on + strand, within Synpcc7942_0457 at 445.938 kb on + strand, within Synpcc7942_0457 at 445.941 kb on - strand, within Synpcc7942_0457 at 445.951 kb on - strand, within Synpcc7942_0457 at 445.988 kb on - strand, within Synpcc7942_0457 at 446.071 kb on + strand, within Synpcc7942_0457 at 446.120 kb on + strand at 446.169 kb on + strand at 446.169 kb on + strand at 446.185 kb on + strand at 446.200 kb on - strand at 446.234 kb on + strand at 446.242 kb on - strand at 446.287 kb on + strand at 446.318 kb on - strand, within fghA at 446.324 kb on - strand, within fghA at 446.337 kb on - strand, within fghA at 446.343 kb on + strand, within fghA at 446.453 kb on + strand, within fghA at 446.468 kb on - strand, within fghA at 446.477 kb on + strand, within fghA at 446.499 kb on - strand, within fghA at 446.507 kb on + strand, within fghA at 446.515 kb on - strand, within fghA at 446.533 kb on + strand, within fghA at 446.545 kb on + strand, within fghA at 446.553 kb on - strand, within fghA at 446.570 kb on + strand, within fghA at 446.578 kb on - strand, within fghA at 446.580 kb on + strand, within fghA at 446.588 kb on - strand, within fghA at 446.595 kb on - strand, within fghA at 446.665 kb on + strand, within fghA at 446.700 kb on + strand, within fghA at 446.708 kb on - strand, within fghA at 446.710 kb on - strand, within fghA at 446.741 kb on + strand, within fghA at 446.749 kb on - strand, within fghA at 446.782 kb on + strand, within fghA at 446.888 kb on + strand, within fghA at 446.896 kb on - strand, within fghA at 446.901 kb on - strand, within fghA at 446.901 kb on - strand, within fghA at 446.909 kb on + strand, within fghA at 446.917 kb on - strand, within fghA at 446.930 kb on - strand, within fghA at 446.930 kb on - strand, within fghA at 446.932 kb on - strand, within fghA at 446.947 kb on - strand, within fghA at 446.960 kb on - strand, within fghA at 446.965 kb on - strand, within fghA at 446.977 kb on + strand at 446.979 kb on + strand at 446.984 kb on + strand at 446.985 kb on - strand at 446.991 kb on + strand at 446.999 kb on - strand at 447.007 kb on - strand at 447.035 kb on + strand at 447.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in Fresh BG11R in Flasks with Spin rep 1 remove 444,576 - tgt Synpcc7942_0455 0.50 +1.0 444,595 + tgt Synpcc7942_0455 0.51 -0.0 444,595 + tgt Synpcc7942_0455 0.51 +1.0 444,595 + tgt Synpcc7942_0455 0.51 -1.2 444,595 + tgt Synpcc7942_0455 0.51 +1.0 444,603 - tgt Synpcc7942_0455 0.52 +1.0 444,603 - tgt Synpcc7942_0455 0.52 +1.0 444,603 - tgt Synpcc7942_0455 0.52 +1.0 444,603 - tgt Synpcc7942_0455 0.52 -1.6 444,738 + tgt Synpcc7942_0455 0.64 -0.6 444,749 + tgt Synpcc7942_0455 0.65 -0.4 444,749 + tgt Synpcc7942_0455 0.65 -1.3 444,749 + tgt Synpcc7942_0455 0.65 -0.0 444,757 - tgt Synpcc7942_0455 0.65 +1.0 444,773 + tgt Synpcc7942_0455 0.67 -1.0 444,796 + tgt Synpcc7942_0455 0.69 +1.0 444,858 + tgt Synpcc7942_0455 0.74 -0.0 444,858 + tgt Synpcc7942_0455 0.74 +1.0 444,871 - tgt Synpcc7942_0455 0.75 -1.8 444,902 + tgt Synpcc7942_0455 0.78 +0.6 444,948 + tgt Synpcc7942_0455 0.82 -2.6 445,014 + tgt Synpcc7942_0455 0.88 +1.0 445,022 - tgt Synpcc7942_0455 0.88 +1.0 445,092 - +1.0 445,116 + +2.0 445,129 - +0.2 445,129 - -2.5 445,148 - -0.0 445,208 + -0.0 445,218 + +1.0 445,293 - psbK Synpcc7942_0456 0.61 +1.0 445,349 - -1.2 445,356 - -0.5 445,364 - -2.0 445,395 + -2.6 445,446 - -1.8 445,573 - -0.6 445,583 + -0.1 445,593 + -0.6 445,601 - -1.3 445,641 + Synpcc7942_0457 0.13 +2.0 445,670 + Synpcc7942_0457 0.17 +1.0 445,670 + +0.3 445,670 + Synpcc7942_0457 0.17 -0.2 445,670 + Synpcc7942_0457 0.17 -0.3 445,675 + -0.5 445,678 - Synpcc7942_0457 0.19 +0.2 445,678 - Synpcc7942_0457 0.19 -0.8 445,678 - Synpcc7942_0457 0.19 -0.6 445,678 - Synpcc7942_0457 0.19 -0.8 445,681 + Synpcc7942_0457 0.19 +2.2 445,686 + Synpcc7942_0457 0.20 -0.2 445,689 - Synpcc7942_0457 0.21 +2.2 445,691 - Synpcc7942_0457 0.21 +4.5 445,755 + Synpcc7942_0457 0.31 -0.2 445,763 - Synpcc7942_0457 0.33 +1.0 445,771 + Synpcc7942_0457 0.34 -1.0 445,783 + Synpcc7942_0457 0.36 -1.7 445,790 + Synpcc7942_0457 0.37 -0.2 445,798 - Synpcc7942_0457 0.38 -1.9 445,799 - Synpcc7942_0457 0.38 -1.1 445,806 + Synpcc7942_0457 0.39 -0.4 445,814 - Synpcc7942_0457 0.41 +0.2 445,832 + Synpcc7942_0457 0.44 +1.3 445,874 - Synpcc7942_0457 0.50 -1.1 445,897 + Synpcc7942_0457 0.54 -0.4 445,900 - Synpcc7942_0457 0.55 -0.6 445,933 + Synpcc7942_0457 0.60 -0.0 445,933 + Synpcc7942_0457 0.60 +0.6 445,933 + Synpcc7942_0457 0.60 +0.3 445,938 + Synpcc7942_0457 0.61 -2.1 445,941 - Synpcc7942_0457 0.61 -2.2 445,951 - Synpcc7942_0457 0.63 +1.0 445,988 - Synpcc7942_0457 0.69 -2.6 446,071 + Synpcc7942_0457 0.82 +1.0 446,120 + +1.5 446,169 + +3.6 446,169 + +2.3 446,185 + -0.3 446,200 - +2.2 446,234 + -0.0 446,242 - +0.4 446,287 + -1.8 446,318 - fghA Synpcc7942_0458 0.12 -2.9 446,324 - fghA Synpcc7942_0458 0.13 -0.0 446,337 - fghA Synpcc7942_0458 0.15 +1.4 446,343 + fghA Synpcc7942_0458 0.15 +1.0 446,453 + fghA Synpcc7942_0458 0.28 -2.7 446,468 - fghA Synpcc7942_0458 0.30 -1.1 446,477 + fghA Synpcc7942_0458 0.31 -0.0 446,499 - fghA Synpcc7942_0458 0.34 +1.0 446,507 + fghA Synpcc7942_0458 0.35 -0.3 446,515 - fghA Synpcc7942_0458 0.36 +0.4 446,533 + fghA Synpcc7942_0458 0.38 +0.3 446,545 + fghA Synpcc7942_0458 0.39 -1.0 446,553 - fghA Synpcc7942_0458 0.40 -1.4 446,570 + fghA Synpcc7942_0458 0.42 -1.0 446,578 - fghA Synpcc7942_0458 0.43 -0.0 446,580 + fghA Synpcc7942_0458 0.44 +1.5 446,588 - fghA Synpcc7942_0458 0.45 -2.8 446,595 - fghA Synpcc7942_0458 0.45 -2.3 446,665 + fghA Synpcc7942_0458 0.54 -0.4 446,700 + fghA Synpcc7942_0458 0.58 +1.0 446,708 - fghA Synpcc7942_0458 0.59 +0.5 446,710 - fghA Synpcc7942_0458 0.59 -2.2 446,741 + fghA Synpcc7942_0458 0.63 +1.7 446,749 - fghA Synpcc7942_0458 0.64 -1.3 446,782 + fghA Synpcc7942_0458 0.68 -1.0 446,888 + fghA Synpcc7942_0458 0.80 -0.4 446,896 - fghA Synpcc7942_0458 0.81 -1.4 446,901 - fghA Synpcc7942_0458 0.82 +2.0 446,901 - fghA Synpcc7942_0458 0.82 +0.8 446,909 + fghA Synpcc7942_0458 0.83 -1.0 446,917 - fghA Synpcc7942_0458 0.84 +2.0 446,930 - fghA Synpcc7942_0458 0.85 -3.3 446,930 - fghA Synpcc7942_0458 0.85 -2.0 446,932 - fghA Synpcc7942_0458 0.86 +0.4 446,947 - fghA Synpcc7942_0458 0.87 -0.3 446,960 - fghA Synpcc7942_0458 0.89 -1.4 446,965 - fghA Synpcc7942_0458 0.90 +5.8 446,977 + -1.4 446,979 + +2.5 446,984 + +1.0 446,985 - -1.8 446,991 + -0.6 446,999 - -0.6 447,007 - -1.6 447,035 + +1.0 447,064 - +1.0
Or see this region's nucleotide sequence