Experiment: Biofilm in Fresh BG11R in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt serS and nifU are separated by 43 nucleotides nifU and Synpcc7942_0451 are separated by 374 nucleotides Synpcc7942_0451 and Synpcc7942_0452 are separated by 57 nucleotides Synpcc7942_0452 and Synpcc7942_0453 are separated by 185 nucleotides
Synpcc7942_0449: serS - seryl-tRNA synthetase, at 438,675 to 440,006
serS
Synpcc7942_0450: nifU - putative NifU-like protein, at 440,050 to 440,295
nifU
Synpcc7942_0451: Synpcc7942_0451 - esterase, at 440,670 to 441,299
_0451
Synpcc7942_0452: Synpcc7942_0452 - hypothetical protein, at 441,357 to 441,836
_0452
Synpcc7942_0453: Synpcc7942_0453 - GAF sensor signal transduction histidine kinase, at 442,022 to 443,236
_0453
Position (kb)
440
441
442 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 440.033 kb on + strand at 440.036 kb on - strand at 440.354 kb on + strand at 440.362 kb on - strand at 440.368 kb on - strand at 440.382 kb on + strand at 440.390 kb on + strand at 440.390 kb on - strand at 440.462 kb on + strand at 440.489 kb on - strand at 440.491 kb on + strand at 440.501 kb on + strand at 440.521 kb on + strand at 440.521 kb on + strand at 440.521 kb on + strand at 440.528 kb on + strand at 440.529 kb on - strand at 440.529 kb on - strand at 440.645 kb on + strand at 440.653 kb on + strand at 440.661 kb on - strand at 440.661 kb on - strand at 440.665 kb on + strand at 440.665 kb on + strand at 440.742 kb on - strand, within Synpcc7942_0451 at 440.742 kb on - strand, within Synpcc7942_0451 at 440.753 kb on + strand, within Synpcc7942_0451 at 440.759 kb on + strand, within Synpcc7942_0451 at 440.790 kb on + strand, within Synpcc7942_0451 at 440.798 kb on + strand, within Synpcc7942_0451 at 440.798 kb on - strand, within Synpcc7942_0451 at 440.798 kb on - strand, within Synpcc7942_0451 at 440.849 kb on + strand, within Synpcc7942_0451 at 440.860 kb on + strand at 440.860 kb on + strand, within Synpcc7942_0451 at 440.860 kb on + strand, within Synpcc7942_0451 at 440.864 kb on + strand, within Synpcc7942_0451 at 440.868 kb on - strand, within Synpcc7942_0451 at 440.868 kb on - strand, within Synpcc7942_0451 at 440.868 kb on - strand, within Synpcc7942_0451 at 440.878 kb on + strand, within Synpcc7942_0451 at 440.883 kb on + strand, within Synpcc7942_0451 at 440.886 kb on - strand, within Synpcc7942_0451 at 440.914 kb on - strand, within Synpcc7942_0451 at 440.964 kb on + strand, within Synpcc7942_0451 at 440.975 kb on - strand, within Synpcc7942_0451 at 441.020 kb on - strand, within Synpcc7942_0451 at 441.039 kb on - strand, within Synpcc7942_0451 at 441.049 kb on - strand, within Synpcc7942_0451 at 441.068 kb on + strand, within Synpcc7942_0451 at 441.074 kb on + strand, within Synpcc7942_0451 at 441.074 kb on + strand, within Synpcc7942_0451 at 441.082 kb on - strand, within Synpcc7942_0451 at 441.082 kb on - strand, within Synpcc7942_0451 at 441.106 kb on + strand, within Synpcc7942_0451 at 441.144 kb on + strand, within Synpcc7942_0451 at 441.194 kb on + strand, within Synpcc7942_0451 at 441.196 kb on + strand, within Synpcc7942_0451 at 441.202 kb on - strand, within Synpcc7942_0451 at 441.204 kb on - strand, within Synpcc7942_0451 at 441.204 kb on - strand, within Synpcc7942_0451 at 441.204 kb on - strand, within Synpcc7942_0451 at 441.209 kb on - strand, within Synpcc7942_0451 at 441.235 kb on + strand, within Synpcc7942_0451 at 441.272 kb on + strand at 441.280 kb on - strand at 441.440 kb on + strand, within Synpcc7942_0452 at 441.505 kb on + strand, within Synpcc7942_0452 at 441.573 kb on - strand, within Synpcc7942_0452 at 441.765 kb on + strand, within Synpcc7942_0452 at 441.860 kb on + strand at 441.915 kb on + strand at 441.924 kb on + strand at 441.924 kb on + strand at 441.936 kb on + strand at 441.937 kb on + strand at 441.967 kb on - strand at 441.991 kb on - strand at 441.992 kb on + strand at 442.004 kb on + strand at 442.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in Fresh BG11R in Flasks with Spin rep 1 remove 440,033 + -1.0 440,036 - -1.6 440,354 + +1.0 440,362 - -0.5 440,368 - -0.4 440,382 + -0.4 440,390 + -3.0 440,390 - -1.0 440,462 + -1.1 440,489 - -1.1 440,491 + +1.0 440,501 + -2.5 440,521 + -0.2 440,521 + +0.6 440,521 + -1.8 440,528 + -0.9 440,529 - +0.6 440,529 - -0.8 440,645 + -0.4 440,653 + -1.1 440,661 - +1.0 440,661 - +0.2 440,665 + -0.8 440,665 + -1.2 440,742 - Synpcc7942_0451 0.11 +0.8 440,742 - Synpcc7942_0451 0.11 -0.6 440,753 + Synpcc7942_0451 0.13 -2.7 440,759 + Synpcc7942_0451 0.14 -0.4 440,790 + Synpcc7942_0451 0.19 -2.5 440,798 + Synpcc7942_0451 0.20 -0.3 440,798 - Synpcc7942_0451 0.20 -1.7 440,798 - Synpcc7942_0451 0.20 -0.2 440,849 + Synpcc7942_0451 0.28 +1.7 440,860 + -1.8 440,860 + Synpcc7942_0451 0.30 +2.6 440,860 + Synpcc7942_0451 0.30 -1.0 440,864 + Synpcc7942_0451 0.31 -0.8 440,868 - Synpcc7942_0451 0.31 +2.0 440,868 - Synpcc7942_0451 0.31 -1.6 440,868 - Synpcc7942_0451 0.31 -0.0 440,878 + Synpcc7942_0451 0.33 +1.5 440,883 + Synpcc7942_0451 0.34 +0.6 440,886 - Synpcc7942_0451 0.34 -1.7 440,914 - Synpcc7942_0451 0.39 -1.6 440,964 + Synpcc7942_0451 0.47 +1.0 440,975 - Synpcc7942_0451 0.48 +1.0 441,020 - Synpcc7942_0451 0.56 -0.1 441,039 - Synpcc7942_0451 0.59 -0.7 441,049 - Synpcc7942_0451 0.60 +3.4 441,068 + Synpcc7942_0451 0.63 -0.0 441,074 + Synpcc7942_0451 0.64 -0.8 441,074 + Synpcc7942_0451 0.64 -3.2 441,082 - Synpcc7942_0451 0.65 +0.2 441,082 - Synpcc7942_0451 0.65 +0.1 441,106 + Synpcc7942_0451 0.69 -0.3 441,144 + Synpcc7942_0451 0.75 +1.3 441,194 + Synpcc7942_0451 0.83 +1.0 441,196 + Synpcc7942_0451 0.83 -0.5 441,202 - Synpcc7942_0451 0.84 +0.4 441,204 - Synpcc7942_0451 0.85 +2.0 441,204 - Synpcc7942_0451 0.85 -0.9 441,204 - Synpcc7942_0451 0.85 -0.6 441,209 - Synpcc7942_0451 0.86 -1.0 441,235 + Synpcc7942_0451 0.90 +1.6 441,272 + -0.6 441,280 - -1.1 441,440 + Synpcc7942_0452 0.17 -1.3 441,505 + Synpcc7942_0452 0.31 -1.3 441,573 - Synpcc7942_0452 0.45 -1.8 441,765 + Synpcc7942_0452 0.85 +1.0 441,860 + +1.4 441,915 + -0.4 441,924 + -2.5 441,924 + -1.3 441,936 + -2.2 441,937 + -1.0 441,967 - -0.6 441,991 - +1.8 441,992 + +0.4 442,004 + +1.0 442,012 - +0.1
Or see this region's nucleotide sequence