Experiment: Biofilm in Fresh BG11R in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt chpX and ndhF4 are separated by 30 nucleotides ndhF4 and ispE are separated by 429 nucleotides
Synpcc7942_0308: chpX - CO2 hydration protein, at 305,969 to 307,108
chpX
Synpcc7942_0309: ndhF4 - NAD(P)H-quinone oxidoreductase subunit F, at 307,139 to 309,043
ndhF4
Synpcc7942_0310: ispE - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 309,473 to 310,417
ispE
Position (kb) 307
308
309
310 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 306.165 kb on + strand, within chpX at 306.186 kb on + strand, within chpX at 306.227 kb on + strand, within chpX at 306.227 kb on + strand, within chpX at 306.264 kb on + strand, within chpX at 306.342 kb on + strand, within chpX at 306.352 kb on - strand, within chpX at 306.377 kb on + strand, within chpX at 306.398 kb on + strand, within chpX at 306.398 kb on + strand, within chpX at 306.400 kb on - strand, within chpX at 306.412 kb on + strand, within chpX at 306.597 kb on - strand, within chpX at 306.606 kb on + strand, within chpX at 306.616 kb on + strand, within chpX at 306.629 kb on + strand, within chpX at 306.637 kb on - strand, within chpX at 306.637 kb on - strand, within chpX at 306.675 kb on + strand, within chpX at 306.687 kb on + strand, within chpX at 306.691 kb on + strand, within chpX at 306.748 kb on + strand, within chpX at 306.804 kb on + strand, within chpX at 306.819 kb on - strand, within chpX at 306.832 kb on - strand, within chpX at 306.862 kb on + strand at 306.941 kb on - strand, within chpX at 306.959 kb on + strand, within chpX at 306.970 kb on + strand, within chpX at 306.972 kb on - strand, within chpX at 306.978 kb on - strand, within chpX at 306.989 kb on + strand, within chpX at 306.994 kb on - strand, within chpX at 306.994 kb on - strand, within chpX at 307.017 kb on + strand at 307.035 kb on - strand at 307.084 kb on - strand at 307.085 kb on + strand at 307.087 kb on + strand at 307.088 kb on - strand at 307.095 kb on - strand at 307.139 kb on - strand at 307.169 kb on - strand at 307.169 kb on - strand at 307.195 kb on + strand at 307.209 kb on - strand at 307.209 kb on - strand at 307.213 kb on + strand at 307.216 kb on - strand at 307.286 kb on - strand at 307.286 kb on - strand at 307.296 kb on + strand at 307.323 kb on + strand at 307.331 kb on - strand, within ndhF4 at 307.331 kb on - strand, within ndhF4 at 307.356 kb on + strand, within ndhF4 at 307.356 kb on + strand, within ndhF4 at 307.356 kb on + strand, within ndhF4 at 307.356 kb on + strand, within ndhF4 at 307.364 kb on - strand, within ndhF4 at 307.364 kb on - strand, within ndhF4 at 307.384 kb on - strand, within ndhF4 at 307.385 kb on + strand, within ndhF4 at 307.413 kb on - strand, within ndhF4 at 307.453 kb on - strand at 307.453 kb on - strand, within ndhF4 at 307.455 kb on + strand, within ndhF4 at 307.455 kb on + strand, within ndhF4 at 307.506 kb on - strand, within ndhF4 at 307.512 kb on - strand, within ndhF4 at 307.583 kb on + strand, within ndhF4 at 307.596 kb on - strand, within ndhF4 at 307.680 kb on + strand, within ndhF4 at 307.688 kb on + strand, within ndhF4 at 307.688 kb on - strand, within ndhF4 at 307.690 kb on + strand, within ndhF4 at 307.718 kb on + strand at 307.726 kb on - strand, within ndhF4 at 307.804 kb on + strand, within ndhF4 at 307.878 kb on + strand, within ndhF4 at 307.881 kb on - strand, within ndhF4 at 307.888 kb on - strand, within ndhF4 at 307.927 kb on + strand, within ndhF4 at 308.002 kb on + strand, within ndhF4 at 308.004 kb on + strand, within ndhF4 at 308.004 kb on + strand, within ndhF4 at 308.012 kb on - strand, within ndhF4 at 308.028 kb on + strand, within ndhF4 at 308.038 kb on + strand, within ndhF4 at 308.046 kb on - strand, within ndhF4 at 308.046 kb on - strand, within ndhF4 at 308.067 kb on + strand, within ndhF4 at 308.067 kb on - strand, within ndhF4 at 308.099 kb on - strand, within ndhF4 at 308.100 kb on - strand, within ndhF4 at 308.140 kb on + strand, within ndhF4 at 308.188 kb on + strand, within ndhF4 at 308.193 kb on - strand, within ndhF4 at 308.196 kb on - strand, within ndhF4 at 308.217 kb on - strand, within ndhF4 at 308.295 kb on - strand, within ndhF4 at 308.297 kb on + strand, within ndhF4 at 308.297 kb on + strand, within ndhF4 at 308.318 kb on + strand, within ndhF4 at 308.428 kb on - strand, within ndhF4 at 308.446 kb on - strand, within ndhF4 at 308.459 kb on + strand, within ndhF4 at 308.472 kb on - strand, within ndhF4 at 308.493 kb on + strand, within ndhF4 at 308.538 kb on + strand, within ndhF4 at 308.572 kb on + strand, within ndhF4 at 308.728 kb on + strand, within ndhF4 at 308.731 kb on - strand, within ndhF4 at 308.736 kb on + strand at 308.736 kb on - strand, within ndhF4 at 308.736 kb on - strand, within ndhF4 at 308.738 kb on + strand, within ndhF4 at 308.741 kb on - strand, within ndhF4 at 308.785 kb on + strand, within ndhF4 at 308.801 kb on - strand, within ndhF4 at 308.825 kb on - strand, within ndhF4 at 308.848 kb on - strand, within ndhF4 at 308.848 kb on - strand, within ndhF4 at 308.864 kb on + strand at 308.941 kb on - strand at 308.964 kb on + strand at 308.964 kb on + strand at 308.972 kb on - strand at 309.114 kb on - strand at 309.131 kb on + strand at 309.139 kb on - strand at 309.165 kb on - strand at 309.165 kb on - strand at 309.184 kb on + strand at 309.193 kb on - strand at 309.215 kb on - strand at 309.220 kb on + strand at 309.261 kb on - strand at 309.287 kb on + strand at 309.287 kb on + strand at 309.296 kb on - strand at 309.323 kb on + strand at 309.344 kb on + strand at 309.355 kb on + strand at 309.359 kb on - strand at 309.439 kb on + strand at 309.486 kb on - strand at 309.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in Fresh BG11R in Flasks with Spin rep 1 remove 306,165 + chpX Synpcc7942_0308 0.17 -0.6 306,186 + chpX Synpcc7942_0308 0.19 +1.4 306,227 + chpX Synpcc7942_0308 0.23 -1.8 306,227 + chpX Synpcc7942_0308 0.23 +0.8 306,264 + chpX Synpcc7942_0308 0.26 -0.0 306,342 + chpX Synpcc7942_0308 0.33 -0.5 306,352 - chpX Synpcc7942_0308 0.34 -2.3 306,377 + chpX Synpcc7942_0308 0.36 -0.0 306,398 + chpX Synpcc7942_0308 0.38 -2.2 306,398 + chpX Synpcc7942_0308 0.38 -1.4 306,400 - chpX Synpcc7942_0308 0.38 +0.4 306,412 + chpX Synpcc7942_0308 0.39 -0.3 306,597 - chpX Synpcc7942_0308 0.55 -0.6 306,606 + chpX Synpcc7942_0308 0.56 -2.3 306,616 + chpX Synpcc7942_0308 0.57 -1.9 306,629 + chpX Synpcc7942_0308 0.58 +0.1 306,637 - chpX Synpcc7942_0308 0.59 -0.8 306,637 - chpX Synpcc7942_0308 0.59 -0.0 306,675 + chpX Synpcc7942_0308 0.62 +1.8 306,687 + chpX Synpcc7942_0308 0.63 +1.0 306,691 + chpX Synpcc7942_0308 0.63 +1.0 306,748 + chpX Synpcc7942_0308 0.68 -2.8 306,804 + chpX Synpcc7942_0308 0.73 -0.0 306,819 - chpX Synpcc7942_0308 0.75 +2.4 306,832 - chpX Synpcc7942_0308 0.76 -1.3 306,862 + -1.4 306,941 - chpX Synpcc7942_0308 0.85 -0.3 306,959 + chpX Synpcc7942_0308 0.87 -0.0 306,970 + chpX Synpcc7942_0308 0.88 +2.0 306,972 - chpX Synpcc7942_0308 0.88 -0.0 306,978 - chpX Synpcc7942_0308 0.89 -0.0 306,989 + chpX Synpcc7942_0308 0.89 -1.6 306,994 - chpX Synpcc7942_0308 0.90 -0.6 306,994 - chpX Synpcc7942_0308 0.90 +1.0 307,017 + +1.0 307,035 - -2.9 307,084 - +0.4 307,085 + -1.5 307,087 + -1.7 307,088 - -4.3 307,095 - -0.6 307,139 - -0.3 307,169 - -0.0 307,169 - +0.7 307,195 + -2.0 307,209 - -1.2 307,209 - -2.3 307,213 + -0.0 307,216 - -0.9 307,286 - -1.9 307,286 - -0.4 307,296 + +1.5 307,323 + +1.5 307,331 - ndhF4 Synpcc7942_0309 0.10 -0.6 307,331 - ndhF4 Synpcc7942_0309 0.10 -0.4 307,356 + ndhF4 Synpcc7942_0309 0.11 -1.5 307,356 + ndhF4 Synpcc7942_0309 0.11 +1.0 307,356 + ndhF4 Synpcc7942_0309 0.11 -2.6 307,356 + ndhF4 Synpcc7942_0309 0.11 +1.0 307,364 - ndhF4 Synpcc7942_0309 0.12 +1.0 307,364 - ndhF4 Synpcc7942_0309 0.12 -2.4 307,384 - ndhF4 Synpcc7942_0309 0.13 -1.5 307,385 + ndhF4 Synpcc7942_0309 0.13 +1.3 307,413 - ndhF4 Synpcc7942_0309 0.14 -2.3 307,453 - +1.0 307,453 - ndhF4 Synpcc7942_0309 0.16 +3.8 307,455 + ndhF4 Synpcc7942_0309 0.17 +0.2 307,455 + ndhF4 Synpcc7942_0309 0.17 -0.0 307,506 - ndhF4 Synpcc7942_0309 0.19 -2.2 307,512 - ndhF4 Synpcc7942_0309 0.20 -0.7 307,583 + ndhF4 Synpcc7942_0309 0.23 -0.5 307,596 - ndhF4 Synpcc7942_0309 0.24 -3.8 307,680 + ndhF4 Synpcc7942_0309 0.28 -2.0 307,688 + ndhF4 Synpcc7942_0309 0.29 -1.2 307,688 - ndhF4 Synpcc7942_0309 0.29 -1.9 307,690 + ndhF4 Synpcc7942_0309 0.29 +0.5 307,718 + +1.0 307,726 - ndhF4 Synpcc7942_0309 0.31 -0.0 307,804 + ndhF4 Synpcc7942_0309 0.35 -3.7 307,878 + ndhF4 Synpcc7942_0309 0.39 -1.9 307,881 - ndhF4 Synpcc7942_0309 0.39 +1.7 307,888 - ndhF4 Synpcc7942_0309 0.39 +0.1 307,927 + ndhF4 Synpcc7942_0309 0.41 +1.0 308,002 + ndhF4 Synpcc7942_0309 0.45 +1.0 308,004 + ndhF4 Synpcc7942_0309 0.45 -0.3 308,004 + ndhF4 Synpcc7942_0309 0.45 -3.3 308,012 - ndhF4 Synpcc7942_0309 0.46 +1.0 308,028 + ndhF4 Synpcc7942_0309 0.47 -1.0 308,038 + ndhF4 Synpcc7942_0309 0.47 +1.0 308,046 - ndhF4 Synpcc7942_0309 0.48 -1.7 308,046 - ndhF4 Synpcc7942_0309 0.48 -1.2 308,067 + ndhF4 Synpcc7942_0309 0.49 -1.3 308,067 - ndhF4 Synpcc7942_0309 0.49 -0.1 308,099 - ndhF4 Synpcc7942_0309 0.50 -0.5 308,100 - ndhF4 Synpcc7942_0309 0.50 +1.6 308,140 + ndhF4 Synpcc7942_0309 0.53 -0.0 308,188 + ndhF4 Synpcc7942_0309 0.55 -2.5 308,193 - ndhF4 Synpcc7942_0309 0.55 -1.4 308,196 - ndhF4 Synpcc7942_0309 0.55 -0.9 308,217 - ndhF4 Synpcc7942_0309 0.57 -0.7 308,295 - ndhF4 Synpcc7942_0309 0.61 -2.5 308,297 + ndhF4 Synpcc7942_0309 0.61 -0.6 308,297 + ndhF4 Synpcc7942_0309 0.61 -1.4 308,318 + ndhF4 Synpcc7942_0309 0.62 +1.0 308,428 - ndhF4 Synpcc7942_0309 0.68 -2.5 308,446 - ndhF4 Synpcc7942_0309 0.69 +0.7 308,459 + ndhF4 Synpcc7942_0309 0.69 -1.2 308,472 - ndhF4 Synpcc7942_0309 0.70 -0.0 308,493 + ndhF4 Synpcc7942_0309 0.71 -1.2 308,538 + ndhF4 Synpcc7942_0309 0.73 +2.0 308,572 + ndhF4 Synpcc7942_0309 0.75 -2.7 308,728 + ndhF4 Synpcc7942_0309 0.83 -0.6 308,731 - ndhF4 Synpcc7942_0309 0.84 +0.7 308,736 + -0.9 308,736 - ndhF4 Synpcc7942_0309 0.84 -1.4 308,736 - ndhF4 Synpcc7942_0309 0.84 -0.7 308,738 + ndhF4 Synpcc7942_0309 0.84 -0.5 308,741 - ndhF4 Synpcc7942_0309 0.84 -0.0 308,785 + ndhF4 Synpcc7942_0309 0.86 -0.0 308,801 - ndhF4 Synpcc7942_0309 0.87 -1.3 308,825 - ndhF4 Synpcc7942_0309 0.89 -0.6 308,848 - ndhF4 Synpcc7942_0309 0.90 -0.6 308,848 - ndhF4 Synpcc7942_0309 0.90 -2.5 308,864 + +1.0 308,941 - -1.0 308,964 + +1.0 308,964 + -1.4 308,972 - +0.6 309,114 - -0.6 309,131 + +1.0 309,139 - +1.0 309,165 - -2.5 309,165 - -1.0 309,184 + +3.0 309,193 - -0.3 309,215 - -5.1 309,220 + -0.9 309,261 - -0.9 309,287 + -2.3 309,287 + -1.1 309,296 - -0.6 309,323 + -2.2 309,344 + +0.2 309,355 + -0.3 309,359 - -1.5 309,439 + +0.4 309,486 - -0.6 309,489 - +1.0
Or see this region's nucleotide sequence