Experiment: Biofilm in Fresh BG11R in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysM and Synpcc7942_0172 overlap by 4 nucleotides Synpcc7942_0172 and cysQ overlap by 4 nucleotides cysQ and Synpcc7942_0174 are separated by 39 nucleotides Synpcc7942_0174 and Synpcc7942_0175 overlap by 4 nucleotides
Synpcc7942_0171: cysM - cysteine synthase A, at 170,797 to 171,915
cysM
Synpcc7942_0172: Synpcc7942_0172 - hypothetical protein, at 171,912 to 172,205
_0172
Synpcc7942_0173: cysQ - 3'-Phosphoadenosine 5'-phosphosulfate (PAPS) 3'-phosphatase-like, at 172,202 to 173,071
cysQ
Synpcc7942_0174: Synpcc7942_0174 - hypothetical protein, at 173,111 to 173,989
_0174
Synpcc7942_0175: Synpcc7942_0175 - hypothetical protein, at 173,986 to 174,198
_0175
Position (kb) 172
173
174 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 171.228 kb on + strand, within cysM at 171.244 kb on + strand, within cysM at 171.251 kb on + strand, within cysM at 171.274 kb on + strand, within cysM at 171.285 kb on + strand, within cysM at 171.293 kb on - strand, within cysM at 171.337 kb on + strand, within cysM at 171.361 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.559 kb on + strand at 171.559 kb on + strand, within cysM at 171.567 kb on - strand, within cysM at 171.567 kb on - strand, within cysM at 171.627 kb on - strand, within cysM at 171.687 kb on + strand, within cysM at 171.694 kb on + strand, within cysM at 171.694 kb on + strand, within cysM at 171.695 kb on - strand, within cysM at 171.707 kb on + strand, within cysM at 171.713 kb on + strand, within cysM at 171.725 kb on + strand, within cysM at 171.746 kb on + strand, within cysM at 171.858 kb on + strand at 171.866 kb on - strand at 171.874 kb on - strand at 171.884 kb on - strand at 171.959 kb on + strand, within Synpcc7942_0172 at 171.959 kb on - strand, within Synpcc7942_0172 at 171.965 kb on - strand, within Synpcc7942_0172 at 171.969 kb on + strand, within Synpcc7942_0172 at 172.008 kb on - strand, within Synpcc7942_0172 at 172.061 kb on - strand, within Synpcc7942_0172 at 172.101 kb on + strand, within Synpcc7942_0172 at 172.101 kb on + strand, within Synpcc7942_0172 at 172.101 kb on + strand, within Synpcc7942_0172 at 172.118 kb on - strand, within Synpcc7942_0172 at 172.204 kb on + strand at 172.212 kb on - strand at 172.222 kb on - strand at 172.239 kb on - strand at 172.243 kb on + strand at 172.296 kb on - strand, within cysQ at 172.310 kb on - strand, within cysQ at 172.323 kb on + strand, within cysQ at 172.323 kb on + strand, within cysQ at 172.323 kb on - strand, within cysQ at 172.334 kb on + strand, within cysQ at 172.360 kb on + strand, within cysQ at 172.360 kb on + strand, within cysQ at 172.368 kb on - strand, within cysQ at 172.406 kb on + strand, within cysQ at 172.426 kb on - strand, within cysQ at 172.438 kb on + strand, within cysQ at 172.472 kb on + strand, within cysQ at 172.477 kb on + strand, within cysQ at 172.477 kb on + strand, within cysQ at 172.485 kb on - strand, within cysQ at 172.612 kb on - strand, within cysQ at 172.625 kb on + strand, within cysQ at 172.625 kb on + strand, within cysQ at 172.625 kb on + strand, within cysQ at 172.625 kb on + strand at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.666 kb on + strand, within cysQ at 172.698 kb on + strand, within cysQ at 172.704 kb on - strand, within cysQ at 172.733 kb on + strand, within cysQ at 172.776 kb on + strand, within cysQ at 172.808 kb on - strand, within cysQ at 172.813 kb on - strand, within cysQ at 172.813 kb on - strand at 172.815 kb on + strand, within cysQ at 172.830 kb on + strand, within cysQ at 172.953 kb on + strand, within cysQ at 172.953 kb on + strand, within cysQ at 172.953 kb on + strand, within cysQ at 172.953 kb on + strand, within cysQ at 172.961 kb on - strand, within cysQ at 172.961 kb on - strand, within cysQ at 172.961 kb on - strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.973 kb on + strand, within cysQ at 172.976 kb on - strand, within cysQ at 172.976 kb on - strand, within cysQ at 172.976 kb on - strand, within cysQ at 172.976 kb on - strand, within cysQ at 172.976 kb on - strand, within cysQ at 172.989 kb on - strand at 173.016 kb on - strand at 173.039 kb on - strand at 173.062 kb on - strand at 173.062 kb on - strand at 173.066 kb on + strand at 173.071 kb on - strand at 173.075 kb on + strand at 173.075 kb on + strand at 173.075 kb on + strand at 173.083 kb on - strand at 173.084 kb on - strand at 173.084 kb on - strand at 173.101 kb on + strand at 173.102 kb on - strand at 173.109 kb on - strand at 173.109 kb on - strand at 173.109 kb on - strand at 173.109 kb on - strand at 173.118 kb on + strand at 173.119 kb on + strand at 173.132 kb on - strand at 173.134 kb on + strand at 173.164 kb on + strand at 173.172 kb on - strand at 173.205 kb on + strand, within Synpcc7942_0174 at 173.205 kb on + strand, within Synpcc7942_0174 at 173.205 kb on + strand, within Synpcc7942_0174 at 173.213 kb on - strand, within Synpcc7942_0174 at 173.222 kb on + strand, within Synpcc7942_0174 at 173.259 kb on - strand, within Synpcc7942_0174 at 173.275 kb on + strand, within Synpcc7942_0174 at 173.275 kb on + strand, within Synpcc7942_0174 at 173.301 kb on + strand, within Synpcc7942_0174 at 173.301 kb on + strand, within Synpcc7942_0174 at 173.301 kb on + strand, within Synpcc7942_0174 at 173.309 kb on - strand, within Synpcc7942_0174 at 173.309 kb on - strand, within Synpcc7942_0174 at 173.311 kb on - strand, within Synpcc7942_0174 at 173.311 kb on - strand, within Synpcc7942_0174 at 173.316 kb on + strand, within Synpcc7942_0174 at 173.319 kb on - strand, within Synpcc7942_0174 at 173.324 kb on - strand, within Synpcc7942_0174 at 173.345 kb on + strand, within Synpcc7942_0174 at 173.345 kb on + strand, within Synpcc7942_0174 at 173.345 kb on + strand, within Synpcc7942_0174 at 173.345 kb on + strand, within Synpcc7942_0174 at 173.353 kb on - strand, within Synpcc7942_0174 at 173.353 kb on - strand, within Synpcc7942_0174 at 173.353 kb on - strand, within Synpcc7942_0174 at 173.357 kb on + strand, within Synpcc7942_0174 at 173.357 kb on + strand, within Synpcc7942_0174 at 173.370 kb on - strand, within Synpcc7942_0174 at 173.432 kb on + strand, within Synpcc7942_0174 at 173.433 kb on + strand, within Synpcc7942_0174 at 173.441 kb on - strand, within Synpcc7942_0174 at 173.441 kb on - strand, within Synpcc7942_0174 at 173.467 kb on + strand, within Synpcc7942_0174 at 173.472 kb on + strand, within Synpcc7942_0174 at 173.479 kb on + strand, within Synpcc7942_0174 at 173.479 kb on + strand, within Synpcc7942_0174 at 173.479 kb on + strand, within Synpcc7942_0174 at 173.479 kb on + strand, within Synpcc7942_0174 at 173.479 kb on + strand, within Synpcc7942_0174 at 173.479 kb on + strand, within Synpcc7942_0174 at 173.487 kb on - strand, within Synpcc7942_0174 at 173.487 kb on - strand, within Synpcc7942_0174 at 173.495 kb on - strand, within Synpcc7942_0174 at 173.495 kb on - strand, within Synpcc7942_0174 at 173.534 kb on + strand, within Synpcc7942_0174 at 173.593 kb on + strand, within Synpcc7942_0174 at 173.597 kb on + strand, within Synpcc7942_0174 at 173.610 kb on + strand, within Synpcc7942_0174 at 173.628 kb on + strand, within Synpcc7942_0174 at 173.628 kb on + strand, within Synpcc7942_0174 at 173.672 kb on + strand, within Synpcc7942_0174 at 173.672 kb on + strand, within Synpcc7942_0174 at 173.672 kb on + strand, within Synpcc7942_0174 at 173.672 kb on + strand, within Synpcc7942_0174 at 173.680 kb on - strand, within Synpcc7942_0174 at 173.715 kb on + strand, within Synpcc7942_0174 at 173.790 kb on + strand, within Synpcc7942_0174 at 173.790 kb on + strand, within Synpcc7942_0174 at 173.807 kb on + strand, within Synpcc7942_0174 at 173.813 kb on - strand, within Synpcc7942_0174 at 173.825 kb on - strand, within Synpcc7942_0174 at 173.846 kb on - strand, within Synpcc7942_0174 at 173.871 kb on + strand, within Synpcc7942_0174 at 173.873 kb on + strand, within Synpcc7942_0174 at 173.905 kb on - strand at 173.960 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in Fresh BG11R in Flasks with Spin rep 1 remove 171,228 + cysM Synpcc7942_0171 0.39 -1.4 171,244 + cysM Synpcc7942_0171 0.40 +2.5 171,251 + cysM Synpcc7942_0171 0.41 -1.2 171,274 + cysM Synpcc7942_0171 0.43 +2.9 171,285 + cysM Synpcc7942_0171 0.44 -0.1 171,293 - cysM Synpcc7942_0171 0.44 -1.7 171,337 + cysM Synpcc7942_0171 0.48 +0.6 171,361 + cysM Synpcc7942_0171 0.50 -0.7 171,515 + cysM Synpcc7942_0171 0.64 -0.1 171,515 + cysM Synpcc7942_0171 0.64 +0.9 171,515 + cysM Synpcc7942_0171 0.64 +0.9 171,515 + +0.2 171,523 - cysM Synpcc7942_0171 0.65 -0.1 171,523 - cysM Synpcc7942_0171 0.65 -0.1 171,523 - cysM Synpcc7942_0171 0.65 +0.9 171,523 - cysM Synpcc7942_0171 0.65 +0.9 171,559 + -0.5 171,559 + cysM Synpcc7942_0171 0.68 -1.1 171,567 - cysM Synpcc7942_0171 0.69 +0.9 171,567 - cysM Synpcc7942_0171 0.69 -1.7 171,627 - cysM Synpcc7942_0171 0.74 -0.1 171,687 + cysM Synpcc7942_0171 0.80 +0.9 171,694 + cysM Synpcc7942_0171 0.80 -0.1 171,694 + cysM Synpcc7942_0171 0.80 -2.8 171,695 - cysM Synpcc7942_0171 0.80 +0.9 171,707 + cysM Synpcc7942_0171 0.81 -0.1 171,713 + cysM Synpcc7942_0171 0.82 -1.4 171,725 + cysM Synpcc7942_0171 0.83 +0.9 171,746 + cysM Synpcc7942_0171 0.85 +0.9 171,858 + -2.9 171,866 - -0.9 171,874 - -1.9 171,884 - +0.9 171,959 + Synpcc7942_0172 0.16 +1.9 171,959 - Synpcc7942_0172 0.16 +1.2 171,965 - Synpcc7942_0172 0.18 -1.4 171,969 + Synpcc7942_0172 0.19 -1.5 172,008 - Synpcc7942_0172 0.33 -1.4 172,061 - Synpcc7942_0172 0.51 -0.1 172,101 + Synpcc7942_0172 0.64 +1.9 172,101 + Synpcc7942_0172 0.64 -0.9 172,101 + Synpcc7942_0172 0.64 +2.6 172,118 - Synpcc7942_0172 0.70 +1.4 172,204 + +2.1 172,212 - -0.6 172,222 - +0.9 172,239 - -1.6 172,243 + -1.1 172,296 - cysQ Synpcc7942_0173 0.11 -1.7 172,310 - cysQ Synpcc7942_0173 0.12 +3.3 172,323 + cysQ Synpcc7942_0173 0.14 -2.0 172,323 + cysQ Synpcc7942_0173 0.14 -1.6 172,323 - cysQ Synpcc7942_0173 0.14 +0.1 172,334 + cysQ Synpcc7942_0173 0.15 -0.8 172,360 + cysQ Synpcc7942_0173 0.18 -1.5 172,360 + cysQ Synpcc7942_0173 0.18 -1.1 172,368 - cysQ Synpcc7942_0173 0.19 -1.3 172,406 + cysQ Synpcc7942_0173 0.23 -1.3 172,426 - cysQ Synpcc7942_0173 0.26 +0.2 172,438 + cysQ Synpcc7942_0173 0.27 -1.8 172,472 + cysQ Synpcc7942_0173 0.31 -3.1 172,477 + cysQ Synpcc7942_0173 0.32 -0.6 172,477 + cysQ Synpcc7942_0173 0.32 +1.7 172,485 - cysQ Synpcc7942_0173 0.33 -2.1 172,612 - cysQ Synpcc7942_0173 0.47 -3.6 172,625 + cysQ Synpcc7942_0173 0.49 +0.9 172,625 + cysQ Synpcc7942_0173 0.49 -1.1 172,625 + cysQ Synpcc7942_0173 0.49 -3.0 172,625 + -0.9 172,631 + cysQ Synpcc7942_0173 0.49 -0.4 172,631 + cysQ Synpcc7942_0173 0.49 -2.1 172,631 + cysQ Synpcc7942_0173 0.49 +0.9 172,631 + cysQ Synpcc7942_0173 0.49 -1.6 172,631 + cysQ Synpcc7942_0173 0.49 -0.5 172,631 + cysQ Synpcc7942_0173 0.49 +0.9 172,631 + -0.3 172,631 + cysQ Synpcc7942_0173 0.49 -3.1 172,631 + cysQ Synpcc7942_0173 0.49 +6.2 172,639 - cysQ Synpcc7942_0173 0.50 -1.3 172,639 - cysQ Synpcc7942_0173 0.50 +0.9 172,639 - cysQ Synpcc7942_0173 0.50 +0.4 172,639 - cysQ Synpcc7942_0173 0.50 -2.7 172,639 - cysQ Synpcc7942_0173 0.50 -0.1 172,639 - cysQ Synpcc7942_0173 0.50 +1.8 172,639 - cysQ Synpcc7942_0173 0.50 -0.9 172,639 - cysQ Synpcc7942_0173 0.50 +1.8 172,639 - cysQ Synpcc7942_0173 0.50 -1.9 172,666 + cysQ Synpcc7942_0173 0.53 -0.1 172,698 + cysQ Synpcc7942_0173 0.57 -2.1 172,704 - cysQ Synpcc7942_0173 0.58 -2.1 172,733 + cysQ Synpcc7942_0173 0.61 -1.2 172,776 + cysQ Synpcc7942_0173 0.66 -2.2 172,808 - cysQ Synpcc7942_0173 0.70 +0.2 172,813 - cysQ Synpcc7942_0173 0.70 -0.1 172,813 - -1.2 172,815 + cysQ Synpcc7942_0173 0.70 -0.7 172,830 + cysQ Synpcc7942_0173 0.72 -1.1 172,953 + cysQ Synpcc7942_0173 0.86 -2.8 172,953 + cysQ Synpcc7942_0173 0.86 -1.2 172,953 + cysQ Synpcc7942_0173 0.86 +1.9 172,953 + cysQ Synpcc7942_0173 0.86 +0.9 172,961 - cysQ Synpcc7942_0173 0.87 -3.0 172,961 - cysQ Synpcc7942_0173 0.87 +0.9 172,961 - cysQ Synpcc7942_0173 0.87 -1.3 172,968 + cysQ Synpcc7942_0173 0.88 -0.4 172,968 + cysQ Synpcc7942_0173 0.88 +1.0 172,968 + cysQ Synpcc7942_0173 0.88 -0.6 172,968 + cysQ Synpcc7942_0173 0.88 +0.6 172,968 + cysQ Synpcc7942_0173 0.88 +0.4 172,968 + cysQ Synpcc7942_0173 0.88 +0.9 172,968 + cysQ Synpcc7942_0173 0.88 -0.3 172,973 + cysQ Synpcc7942_0173 0.89 -1.3 172,976 - cysQ Synpcc7942_0173 0.89 -2.5 172,976 - cysQ Synpcc7942_0173 0.89 -1.8 172,976 - cysQ Synpcc7942_0173 0.89 +2.5 172,976 - cysQ Synpcc7942_0173 0.89 -3.6 172,976 - cysQ Synpcc7942_0173 0.89 -0.1 172,989 - -1.1 173,016 - +0.1 173,039 - +2.9 173,062 - -0.8 173,062 - -1.8 173,066 + -1.0 173,071 - +2.5 173,075 + -0.3 173,075 + -1.9 173,075 + -0.6 173,083 - -2.1 173,084 - -1.1 173,084 - -1.1 173,101 + -0.3 173,102 - +1.5 173,109 - +1.9 173,109 - +0.9 173,109 - -0.1 173,109 - -1.3 173,118 + +0.5 173,119 + +0.9 173,132 - +2.5 173,134 + -2.5 173,164 + -1.0 173,172 - -0.7 173,205 + Synpcc7942_0174 0.11 +0.9 173,205 + Synpcc7942_0174 0.11 -1.9 173,205 + Synpcc7942_0174 0.11 -1.0 173,213 - Synpcc7942_0174 0.12 +0.9 173,222 + Synpcc7942_0174 0.13 -0.1 173,259 - Synpcc7942_0174 0.17 -0.1 173,275 + Synpcc7942_0174 0.19 -2.2 173,275 + Synpcc7942_0174 0.19 -1.4 173,301 + Synpcc7942_0174 0.22 -1.2 173,301 + Synpcc7942_0174 0.22 +0.9 173,301 + Synpcc7942_0174 0.22 +0.1 173,309 - Synpcc7942_0174 0.23 -0.1 173,309 - Synpcc7942_0174 0.23 +1.5 173,311 - Synpcc7942_0174 0.23 -1.0 173,311 - Synpcc7942_0174 0.23 -1.4 173,316 + Synpcc7942_0174 0.23 -1.9 173,319 - Synpcc7942_0174 0.24 -1.5 173,324 - Synpcc7942_0174 0.24 -1.1 173,345 + Synpcc7942_0174 0.27 -1.7 173,345 + Synpcc7942_0174 0.27 +2.7 173,345 + Synpcc7942_0174 0.27 -2.1 173,345 + Synpcc7942_0174 0.27 -1.9 173,353 - Synpcc7942_0174 0.28 +1.9 173,353 - Synpcc7942_0174 0.28 -2.2 173,353 - Synpcc7942_0174 0.28 -1.9 173,357 + Synpcc7942_0174 0.28 -1.1 173,357 + Synpcc7942_0174 0.28 -1.4 173,370 - Synpcc7942_0174 0.29 -0.6 173,432 + Synpcc7942_0174 0.37 -2.5 173,433 + Synpcc7942_0174 0.37 +0.4 173,441 - Synpcc7942_0174 0.38 -0.5 173,441 - Synpcc7942_0174 0.38 -0.6 173,467 + Synpcc7942_0174 0.41 -0.9 173,472 + Synpcc7942_0174 0.41 -0.8 173,479 + Synpcc7942_0174 0.42 +1.9 173,479 + Synpcc7942_0174 0.42 +1.1 173,479 + Synpcc7942_0174 0.42 -2.1 173,479 + Synpcc7942_0174 0.42 -0.6 173,479 + Synpcc7942_0174 0.42 +0.9 173,479 + Synpcc7942_0174 0.42 -1.6 173,487 - Synpcc7942_0174 0.43 -2.0 173,487 - Synpcc7942_0174 0.43 -2.4 173,495 - Synpcc7942_0174 0.44 +0.9 173,495 - Synpcc7942_0174 0.44 -1.6 173,534 + Synpcc7942_0174 0.48 -0.1 173,593 + Synpcc7942_0174 0.55 -1.4 173,597 + Synpcc7942_0174 0.55 -1.4 173,610 + Synpcc7942_0174 0.57 -2.1 173,628 + Synpcc7942_0174 0.59 -1.6 173,628 + Synpcc7942_0174 0.59 +0.9 173,672 + Synpcc7942_0174 0.64 -0.1 173,672 + Synpcc7942_0174 0.64 -1.1 173,672 + Synpcc7942_0174 0.64 +1.0 173,672 + Synpcc7942_0174 0.64 +0.9 173,680 - Synpcc7942_0174 0.65 +0.9 173,715 + Synpcc7942_0174 0.69 -0.6 173,790 + Synpcc7942_0174 0.77 -1.4 173,790 + Synpcc7942_0174 0.77 +0.2 173,807 + Synpcc7942_0174 0.79 +0.0 173,813 - Synpcc7942_0174 0.80 -0.1 173,825 - Synpcc7942_0174 0.81 -0.6 173,846 - Synpcc7942_0174 0.84 +0.4 173,871 + Synpcc7942_0174 0.86 -1.4 173,873 + Synpcc7942_0174 0.87 +0.9 173,905 - -2.9 173,960 + -1.6
Or see this region's nucleotide sequence