Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4748 and BT4749 are separated by 81 nucleotides BT4749 and BT4750 are separated by 111 nucleotides BT4750 and BT4751 are separated by 13 nucleotides BT4751 and BT4752 are separated by 111 nucleotides
BT4748: BT4748 - hypothetical protein (NCBI ptt file), at 6,230,088 to 6,231,110
BT4748
BT4749: BT4749 - putative DNA methylase (NCBI ptt file), at 6,231,192 to 6,235,154
BT4749
BT4750: BT4750 - hypothetical protein (NCBI ptt file), at 6,235,266 to 6,235,463
BT4750
BT4751: BT4751 - hypothetical protein (NCBI ptt file), at 6,235,477 to 6,235,920
BT4751
BT4752: BT4752 - conserved hypothetical protein (NCBI ptt file), at 6,236,032 to 6,237,327
BT4752
Position (kb)
6231
6232
6233
6234
6235
6236 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 6230.259 kb on - strand, within BT4748 at 6230.341 kb on - strand, within BT4748 at 6230.414 kb on + strand at 6230.414 kb on + strand, within BT4748 at 6230.415 kb on - strand, within BT4748 at 6230.486 kb on + strand, within BT4748 at 6230.488 kb on + strand, within BT4748 at 6230.489 kb on - strand, within BT4748 at 6230.489 kb on - strand, within BT4748 at 6230.607 kb on - strand, within BT4748 at 6230.844 kb on - strand, within BT4748 at 6230.944 kb on + strand, within BT4748 at 6230.945 kb on - strand, within BT4748 at 6230.945 kb on - strand, within BT4748 at 6230.976 kb on + strand, within BT4748 at 6231.124 kb on + strand at 6231.125 kb on - strand at 6231.140 kb on + strand at 6231.199 kb on - strand at 6231.374 kb on + strand at 6231.433 kb on + strand at 6231.434 kb on - strand at 6231.438 kb on - strand at 6231.482 kb on - strand at 6231.482 kb on - strand at 6231.485 kb on + strand at 6231.485 kb on + strand at 6231.486 kb on - strand at 6231.490 kb on + strand at 6231.490 kb on + strand at 6231.558 kb on + strand at 6231.592 kb on + strand, within BT4749 at 6231.655 kb on + strand, within BT4749 at 6231.726 kb on + strand, within BT4749 at 6231.726 kb on + strand, within BT4749 at 6231.727 kb on - strand, within BT4749 at 6231.734 kb on - strand, within BT4749 at 6231.734 kb on - strand, within BT4749 at 6232.386 kb on + strand, within BT4749 at 6232.399 kb on - strand, within BT4749 at 6232.399 kb on - strand, within BT4749 at 6232.569 kb on + strand, within BT4749 at 6232.597 kb on + strand, within BT4749 at 6232.722 kb on + strand, within BT4749 at 6232.744 kb on + strand, within BT4749 at 6232.818 kb on + strand, within BT4749 at 6232.823 kb on - strand, within BT4749 at 6232.843 kb on + strand, within BT4749 at 6232.844 kb on - strand, within BT4749 at 6232.959 kb on + strand, within BT4749 at 6233.002 kb on - strand, within BT4749 at 6233.080 kb on + strand, within BT4749 at 6233.081 kb on - strand, within BT4749 at 6233.145 kb on - strand, within BT4749 at 6233.153 kb on + strand, within BT4749 at 6233.196 kb on + strand, within BT4749 at 6233.315 kb on - strand, within BT4749 at 6233.390 kb on - strand, within BT4749 at 6233.716 kb on + strand, within BT4749 at 6233.812 kb on + strand, within BT4749 at 6233.812 kb on + strand, within BT4749 at 6233.812 kb on + strand, within BT4749 at 6233.815 kb on + strand, within BT4749 at 6233.827 kb on + strand, within BT4749 at 6234.079 kb on + strand, within BT4749 at 6234.351 kb on + strand, within BT4749 at 6234.405 kb on + strand, within BT4749 at 6234.491 kb on + strand, within BT4749 at 6234.492 kb on - strand, within BT4749 at 6234.529 kb on + strand, within BT4749 at 6234.560 kb on + strand, within BT4749 at 6234.560 kb on + strand, within BT4749 at 6234.561 kb on - strand, within BT4749 at 6234.575 kb on + strand, within BT4749 at 6234.776 kb on - strand at 6234.826 kb on + strand at 6234.831 kb on - strand at 6234.917 kb on + strand at 6234.945 kb on - strand at 6234.947 kb on + strand at 6234.948 kb on - strand at 6234.950 kb on - strand at 6234.952 kb on + strand at 6234.953 kb on - strand at 6234.960 kb on + strand at 6234.978 kb on + strand at 6235.059 kb on + strand at 6235.180 kb on + strand at 6235.249 kb on + strand at 6235.479 kb on - strand at 6235.573 kb on - strand, within BT4751 at 6235.589 kb on - strand, within BT4751 at 6235.609 kb on + strand, within BT4751 at 6235.766 kb on - strand, within BT4751 at 6235.778 kb on + strand, within BT4751 at 6235.912 kb on - strand at 6235.931 kb on + strand at 6235.944 kb on + strand at 6235.968 kb on - strand at 6235.998 kb on + strand at 6236.000 kb on + strand at 6236.033 kb on + strand at 6236.078 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 6,230,259 - BT4748 0.17 +0.7 6,230,341 - BT4748 0.25 +1.9 6,230,414 + -0.1 6,230,414 + BT4748 0.32 -0.5 6,230,415 - BT4748 0.32 +1.2 6,230,486 + BT4748 0.39 +1.4 6,230,488 + BT4748 0.39 -2.1 6,230,489 - BT4748 0.39 -0.1 6,230,489 - BT4748 0.39 -5.2 6,230,607 - BT4748 0.51 +0.5 6,230,844 - BT4748 0.74 +0.1 6,230,944 + BT4748 0.84 -2.4 6,230,945 - BT4748 0.84 +1.9 6,230,945 - BT4748 0.84 -1.1 6,230,976 + BT4748 0.87 -0.5 6,231,124 + -2.1 6,231,125 - -0.7 6,231,140 + -1.1 6,231,199 - +0.8 6,231,374 + -0.1 6,231,433 + -1.0 6,231,434 - -1.7 6,231,438 - -0.1 6,231,482 - -2.9 6,231,482 - -0.9 6,231,485 + -0.1 6,231,485 + +0.0 6,231,486 - -2.1 6,231,490 + -0.6 6,231,490 + +0.9 6,231,558 + +0.3 6,231,592 + BT4749 0.10 -1.1 6,231,655 + BT4749 0.12 +0.9 6,231,726 + BT4749 0.13 -0.1 6,231,726 + BT4749 0.13 -3.7 6,231,727 - BT4749 0.13 -0.1 6,231,734 - BT4749 0.14 -3.1 6,231,734 - BT4749 0.14 +1.8 6,232,386 + BT4749 0.30 +0.9 6,232,399 - BT4749 0.30 -0.1 6,232,399 - BT4749 0.30 +1.5 6,232,569 + BT4749 0.35 +1.9 6,232,597 + BT4749 0.35 +4.3 6,232,722 + BT4749 0.39 -2.9 6,232,744 + BT4749 0.39 +1.4 6,232,818 + BT4749 0.41 -1.7 6,232,823 - BT4749 0.41 -2.4 6,232,843 + BT4749 0.42 -1.1 6,232,844 - BT4749 0.42 +1.2 6,232,959 + BT4749 0.45 +1.5 6,233,002 - BT4749 0.46 +0.9 6,233,080 + BT4749 0.48 -0.1 6,233,081 - BT4749 0.48 -0.5 6,233,145 - BT4749 0.49 -0.1 6,233,153 + BT4749 0.49 -1.7 6,233,196 + BT4749 0.51 +2.5 6,233,315 - BT4749 0.54 -2.1 6,233,390 - BT4749 0.55 +3.3 6,233,716 + BT4749 0.64 +0.8 6,233,812 + BT4749 0.66 -2.1 6,233,812 + BT4749 0.66 +1.0 6,233,812 + BT4749 0.66 -1.1 6,233,815 + BT4749 0.66 +0.6 6,233,827 + BT4749 0.66 +2.5 6,234,079 + BT4749 0.73 +1.9 6,234,351 + BT4749 0.80 +1.3 6,234,405 + BT4749 0.81 +1.9 6,234,491 + BT4749 0.83 +1.9 6,234,492 - BT4749 0.83 -1.9 6,234,529 + BT4749 0.84 -3.4 6,234,560 + BT4749 0.85 +3.2 6,234,560 + BT4749 0.85 -0.5 6,234,561 - BT4749 0.85 +3.9 6,234,575 + BT4749 0.85 -1.4 6,234,776 - -4.1 6,234,826 + -0.0 6,234,831 - -0.1 6,234,917 + +0.6 6,234,945 - +2.5 6,234,947 + +2.2 6,234,948 - +2.0 6,234,950 - -2.4 6,234,952 + -0.9 6,234,953 - -0.1 6,234,960 + +2.4 6,234,978 + -0.7 6,235,059 + -1.5 6,235,180 + -2.1 6,235,249 + -0.7 6,235,479 - -0.6 6,235,573 - BT4751 0.22 +1.0 6,235,589 - BT4751 0.25 -2.4 6,235,609 + BT4751 0.30 -0.5 6,235,766 - BT4751 0.65 -2.9 6,235,778 + BT4751 0.68 -1.6 6,235,912 - -1.3 6,235,931 + +2.5 6,235,944 + -3.4 6,235,968 - -1.7 6,235,998 + -2.1 6,236,000 + -0.7 6,236,033 + +4.0 6,236,078 - +0.5
Or see this region's nucleotide sequence