Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4736 and BT4737 overlap by 8 nucleotides BT4737 and BT4738 are separated by 13 nucleotides BT4738 and tRNA-Ser are separated by 240 nucleotides tRNA-Ser and BT4739 are separated by 240 nucleotides
BT4736: BT4736 - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 6,213,501 to 6,213,950
BT4736
BT4737: BT4737 - pyruvate formate-lyase activating enzyme (NCBI ptt file), at 6,213,943 to 6,214,671
BT4737
BT4738: BT4738 - formate acetyltransferase (NCBI ptt file), at 6,214,685 to 6,216,913
BT4738
BT_t71: tRNA-Ser - tRNA-Ser (RefSeq), at 6,217,154 to 6,217,238
tRNA-Ser
BT4739: BT4739 - transposase (NCBI ptt file), at 6,217,479 to 6,218,702
BT4739
Position (kb)
6214
6215
6216
6217 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 6213.710 kb on - strand, within BT4736 at 6213.716 kb on - strand, within BT4736 at 6213.716 kb on - strand, within BT4736 at 6213.716 kb on - strand, within BT4736 at 6213.720 kb on + strand, within BT4736 at 6213.764 kb on + strand, within BT4736 at 6213.764 kb on + strand, within BT4736 at 6213.831 kb on - strand, within BT4736 at 6213.836 kb on - strand, within BT4736 at 6213.836 kb on - strand, within BT4736 at 6213.871 kb on - strand, within BT4736 at 6213.884 kb on - strand, within BT4736 at 6213.902 kb on + strand, within BT4736 at 6213.944 kb on + strand at 6213.948 kb on + strand at 6213.960 kb on + strand at 6213.968 kb on + strand at 6214.037 kb on + strand, within BT4737 at 6214.038 kb on - strand, within BT4737 at 6214.062 kb on + strand, within BT4737 at 6214.062 kb on + strand, within BT4737 at 6214.083 kb on - strand, within BT4737 at 6214.167 kb on - strand, within BT4737 at 6214.178 kb on - strand, within BT4737 at 6214.294 kb on + strand, within BT4737 at 6214.297 kb on - strand, within BT4737 at 6214.337 kb on - strand, within BT4737 at 6214.674 kb on - strand at 6214.674 kb on - strand at 6214.674 kb on - strand at 6214.674 kb on - strand at 6214.674 kb on - strand at 6214.691 kb on - strand at 6214.792 kb on - strand at 6214.818 kb on + strand at 6214.820 kb on + strand at 6214.820 kb on + strand at 6214.821 kb on - strand at 6214.825 kb on + strand at 6214.839 kb on - strand at 6215.135 kb on - strand, within BT4738 at 6215.206 kb on - strand, within BT4738 at 6215.232 kb on - strand, within BT4738 at 6215.391 kb on - strand, within BT4738 at 6215.453 kb on - strand, within BT4738 at 6215.454 kb on + strand, within BT4738 at 6215.454 kb on + strand, within BT4738 at 6215.676 kb on - strand, within BT4738 at 6215.761 kb on + strand, within BT4738 at 6215.761 kb on + strand, within BT4738 at 6215.869 kb on - strand, within BT4738 at 6215.912 kb on - strand, within BT4738 at 6215.982 kb on + strand, within BT4738 at 6215.983 kb on - strand at 6216.010 kb on + strand, within BT4738 at 6216.165 kb on + strand, within BT4738 at 6216.172 kb on + strand, within BT4738 at 6216.172 kb on + strand, within BT4738 at 6216.172 kb on + strand, within BT4738 at 6216.174 kb on + strand, within BT4738 at 6216.174 kb on + strand, within BT4738 at 6216.176 kb on + strand, within BT4738 at 6216.185 kb on + strand, within BT4738 at 6216.231 kb on + strand, within BT4738 at 6216.235 kb on - strand, within BT4738 at 6216.421 kb on - strand, within BT4738 at 6216.480 kb on + strand, within BT4738 at 6216.483 kb on - strand, within BT4738 at 6216.483 kb on - strand, within BT4738 at 6216.593 kb on - strand, within BT4738 at 6216.593 kb on - strand, within BT4738 at 6216.670 kb on + strand, within BT4738 at 6216.702 kb on - strand at 6216.707 kb on - strand at 6216.713 kb on - strand at 6216.713 kb on - strand at 6216.716 kb on + strand at 6216.937 kb on - strand at 6216.941 kb on - strand at 6217.312 kb on - strand at 6217.320 kb on + strand at 6217.389 kb on + strand at 6217.390 kb on - strand at 6217.475 kb on + strand at 6217.501 kb on + strand at 6217.509 kb on + strand at 6217.510 kb on - strand at 6217.517 kb on + strand at 6217.735 kb on + strand, within BT4739 at 6217.736 kb on - strand, within BT4739 at 6217.832 kb on + strand, within BT4739 at 6217.832 kb on + strand, within BT4739 at 6217.838 kb on + strand, within BT4739 at 6217.890 kb on + strand, within BT4739 at 6217.891 kb on - strand, within BT4739
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 6,213,710 - BT4736 0.46 -1.7 6,213,716 - BT4736 0.48 -0.1 6,213,716 - BT4736 0.48 +1.9 6,213,716 - BT4736 0.48 -0.9 6,213,720 + BT4736 0.49 +0.4 6,213,764 + BT4736 0.58 -0.3 6,213,764 + BT4736 0.58 -2.2 6,213,831 - BT4736 0.73 +1.5 6,213,836 - BT4736 0.74 -1.0 6,213,836 - BT4736 0.74 -1.4 6,213,871 - BT4736 0.82 +1.9 6,213,884 - BT4736 0.85 -0.1 6,213,902 + BT4736 0.89 -2.4 6,213,944 + -1.5 6,213,948 + -3.1 6,213,960 + -1.7 6,213,968 + +2.7 6,214,037 + BT4737 0.13 -4.0 6,214,038 - BT4737 0.13 -3.0 6,214,062 + BT4737 0.16 -0.7 6,214,062 + BT4737 0.16 -0.1 6,214,083 - BT4737 0.19 -4.4 6,214,167 - BT4737 0.31 +0.5 6,214,178 - BT4737 0.32 -4.6 6,214,294 + BT4737 0.48 -2.9 6,214,297 - BT4737 0.49 -0.1 6,214,337 - BT4737 0.54 -1.9 6,214,674 - -0.7 6,214,674 - -4.1 6,214,674 - -1.5 6,214,674 - +0.5 6,214,674 - +0.5 6,214,691 - -2.5 6,214,792 - -4.1 6,214,818 + -3.8 6,214,820 + -3.3 6,214,820 + -1.7 6,214,821 - -2.4 6,214,825 + -5.7 6,214,839 - +0.5 6,215,135 - BT4738 0.20 -3.1 6,215,206 - BT4738 0.23 -2.6 6,215,232 - BT4738 0.25 -2.1 6,215,391 - BT4738 0.32 -0.1 6,215,453 - BT4738 0.34 -3.8 6,215,454 + BT4738 0.34 -2.7 6,215,454 + BT4738 0.34 -2.1 6,215,676 - BT4738 0.44 -1.1 6,215,761 + BT4738 0.48 -3.6 6,215,761 + BT4738 0.48 -2.7 6,215,869 - BT4738 0.53 -0.1 6,215,912 - BT4738 0.55 -2.1 6,215,982 + BT4738 0.58 -2.9 6,215,983 - -0.1 6,216,010 + BT4738 0.59 -2.4 6,216,165 + BT4738 0.66 -2.7 6,216,172 + BT4738 0.67 -1.7 6,216,172 + BT4738 0.67 -2.1 6,216,172 + BT4738 0.67 -2.0 6,216,174 + BT4738 0.67 +0.5 6,216,174 + BT4738 0.67 -0.1 6,216,176 + BT4738 0.67 -0.1 6,216,185 + BT4738 0.67 -0.1 6,216,231 + BT4738 0.69 -2.7 6,216,235 - BT4738 0.70 -0.1 6,216,421 - BT4738 0.78 -3.0 6,216,480 + BT4738 0.81 -0.1 6,216,483 - BT4738 0.81 -2.1 6,216,483 - BT4738 0.81 -2.4 6,216,593 - BT4738 0.86 -4.1 6,216,593 - BT4738 0.86 -2.4 6,216,670 + BT4738 0.89 -1.7 6,216,702 - -1.5 6,216,707 - -3.1 6,216,713 - -3.8 6,216,713 - -1.1 6,216,716 + -0.8 6,216,937 - -0.7 6,216,941 - -1.0 6,217,312 - -1.2 6,217,320 + -1.7 6,217,389 + -1.7 6,217,390 - -2.4 6,217,475 + -0.7 6,217,501 + -1.1 6,217,509 + -1.1 6,217,510 - -0.2 6,217,517 + -0.1 6,217,735 + BT4739 0.21 +1.5 6,217,736 - BT4739 0.21 -1.3 6,217,832 + BT4739 0.29 -2.9 6,217,832 + BT4739 0.29 -3.1 6,217,838 + BT4739 0.29 -1.7 6,217,890 + BT4739 0.34 -0.1 6,217,891 - BT4739 0.34 +0.9
Or see this region's nucleotide sequence