Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4738

Experiment: BHIS with Trimethoprim 0.00625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4736 and BT4737 overlap by 8 nucleotidesBT4737 and BT4738 are separated by 13 nucleotidesBT4738 and tRNA-Ser are separated by 240 nucleotidestRNA-Ser and BT4739 are separated by 240 nucleotides BT4736: BT4736 - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 6,213,501 to 6,213,950 BT4736 BT4737: BT4737 - pyruvate formate-lyase activating enzyme (NCBI ptt file), at 6,213,943 to 6,214,671 BT4737 BT4738: BT4738 - formate acetyltransferase (NCBI ptt file), at 6,214,685 to 6,216,913 BT4738 BT_t71: tRNA-Ser - tRNA-Ser (RefSeq), at 6,217,154 to 6,217,238 tRNA-Ser BT4739: BT4739 - transposase (NCBI ptt file), at 6,217,479 to 6,218,702 BT4739 Position (kb) 6214 6215 6216 6217Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 6213.710 kb on - strand, within BT4736at 6213.716 kb on - strand, within BT4736at 6213.716 kb on - strand, within BT4736at 6213.716 kb on - strand, within BT4736at 6213.720 kb on + strand, within BT4736at 6213.764 kb on + strand, within BT4736at 6213.764 kb on + strand, within BT4736at 6213.831 kb on - strand, within BT4736at 6213.836 kb on - strand, within BT4736at 6213.836 kb on - strand, within BT4736at 6213.871 kb on - strand, within BT4736at 6213.884 kb on - strand, within BT4736at 6213.902 kb on + strand, within BT4736at 6213.944 kb on + strandat 6213.948 kb on + strandat 6213.960 kb on + strandat 6213.968 kb on + strandat 6214.037 kb on + strand, within BT4737at 6214.038 kb on - strand, within BT4737at 6214.062 kb on + strand, within BT4737at 6214.062 kb on + strand, within BT4737at 6214.083 kb on - strand, within BT4737at 6214.167 kb on - strand, within BT4737at 6214.178 kb on - strand, within BT4737at 6214.294 kb on + strand, within BT4737at 6214.297 kb on - strand, within BT4737at 6214.337 kb on - strand, within BT4737at 6214.674 kb on - strandat 6214.674 kb on - strandat 6214.674 kb on - strandat 6214.674 kb on - strandat 6214.674 kb on - strandat 6214.691 kb on - strandat 6214.792 kb on - strandat 6214.818 kb on + strandat 6214.820 kb on + strandat 6214.820 kb on + strandat 6214.821 kb on - strandat 6214.825 kb on + strandat 6214.839 kb on - strandat 6215.135 kb on - strand, within BT4738at 6215.206 kb on - strand, within BT4738at 6215.232 kb on - strand, within BT4738at 6215.391 kb on - strand, within BT4738at 6215.453 kb on - strand, within BT4738at 6215.454 kb on + strand, within BT4738at 6215.454 kb on + strand, within BT4738at 6215.676 kb on - strand, within BT4738at 6215.761 kb on + strand, within BT4738at 6215.761 kb on + strand, within BT4738at 6215.869 kb on - strand, within BT4738at 6215.912 kb on - strand, within BT4738at 6215.982 kb on + strand, within BT4738at 6215.983 kb on - strandat 6216.010 kb on + strand, within BT4738at 6216.165 kb on + strand, within BT4738at 6216.172 kb on + strand, within BT4738at 6216.172 kb on + strand, within BT4738at 6216.172 kb on + strand, within BT4738at 6216.174 kb on + strand, within BT4738at 6216.174 kb on + strand, within BT4738at 6216.176 kb on + strand, within BT4738at 6216.185 kb on + strand, within BT4738at 6216.231 kb on + strand, within BT4738at 6216.235 kb on - strand, within BT4738at 6216.421 kb on - strand, within BT4738at 6216.480 kb on + strand, within BT4738at 6216.483 kb on - strand, within BT4738at 6216.483 kb on - strand, within BT4738at 6216.593 kb on - strand, within BT4738at 6216.593 kb on - strand, within BT4738at 6216.670 kb on + strand, within BT4738at 6216.702 kb on - strandat 6216.707 kb on - strandat 6216.713 kb on - strandat 6216.713 kb on - strandat 6216.716 kb on + strandat 6216.937 kb on - strandat 6216.941 kb on - strandat 6217.312 kb on - strandat 6217.320 kb on + strandat 6217.389 kb on + strandat 6217.390 kb on - strandat 6217.475 kb on + strandat 6217.501 kb on + strandat 6217.509 kb on + strandat 6217.510 kb on - strandat 6217.517 kb on + strandat 6217.735 kb on + strand, within BT4739at 6217.736 kb on - strand, within BT4739at 6217.832 kb on + strand, within BT4739at 6217.832 kb on + strand, within BT4739at 6217.838 kb on + strand, within BT4739at 6217.890 kb on + strand, within BT4739at 6217.891 kb on - strand, within BT4739

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM
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6,213,710 - BT4736 0.46 -1.7
6,213,716 - BT4736 0.48 -0.1
6,213,716 - BT4736 0.48 +1.9
6,213,716 - BT4736 0.48 -0.9
6,213,720 + BT4736 0.49 +0.4
6,213,764 + BT4736 0.58 -0.3
6,213,764 + BT4736 0.58 -2.2
6,213,831 - BT4736 0.73 +1.5
6,213,836 - BT4736 0.74 -1.0
6,213,836 - BT4736 0.74 -1.4
6,213,871 - BT4736 0.82 +1.9
6,213,884 - BT4736 0.85 -0.1
6,213,902 + BT4736 0.89 -2.4
6,213,944 + -1.5
6,213,948 + -3.1
6,213,960 + -1.7
6,213,968 + +2.7
6,214,037 + BT4737 0.13 -4.0
6,214,038 - BT4737 0.13 -3.0
6,214,062 + BT4737 0.16 -0.7
6,214,062 + BT4737 0.16 -0.1
6,214,083 - BT4737 0.19 -4.4
6,214,167 - BT4737 0.31 +0.5
6,214,178 - BT4737 0.32 -4.6
6,214,294 + BT4737 0.48 -2.9
6,214,297 - BT4737 0.49 -0.1
6,214,337 - BT4737 0.54 -1.9
6,214,674 - -0.7
6,214,674 - -4.1
6,214,674 - -1.5
6,214,674 - +0.5
6,214,674 - +0.5
6,214,691 - -2.5
6,214,792 - -4.1
6,214,818 + -3.8
6,214,820 + -3.3
6,214,820 + -1.7
6,214,821 - -2.4
6,214,825 + -5.7
6,214,839 - +0.5
6,215,135 - BT4738 0.20 -3.1
6,215,206 - BT4738 0.23 -2.6
6,215,232 - BT4738 0.25 -2.1
6,215,391 - BT4738 0.32 -0.1
6,215,453 - BT4738 0.34 -3.8
6,215,454 + BT4738 0.34 -2.7
6,215,454 + BT4738 0.34 -2.1
6,215,676 - BT4738 0.44 -1.1
6,215,761 + BT4738 0.48 -3.6
6,215,761 + BT4738 0.48 -2.7
6,215,869 - BT4738 0.53 -0.1
6,215,912 - BT4738 0.55 -2.1
6,215,982 + BT4738 0.58 -2.9
6,215,983 - -0.1
6,216,010 + BT4738 0.59 -2.4
6,216,165 + BT4738 0.66 -2.7
6,216,172 + BT4738 0.67 -1.7
6,216,172 + BT4738 0.67 -2.1
6,216,172 + BT4738 0.67 -2.0
6,216,174 + BT4738 0.67 +0.5
6,216,174 + BT4738 0.67 -0.1
6,216,176 + BT4738 0.67 -0.1
6,216,185 + BT4738 0.67 -0.1
6,216,231 + BT4738 0.69 -2.7
6,216,235 - BT4738 0.70 -0.1
6,216,421 - BT4738 0.78 -3.0
6,216,480 + BT4738 0.81 -0.1
6,216,483 - BT4738 0.81 -2.1
6,216,483 - BT4738 0.81 -2.4
6,216,593 - BT4738 0.86 -4.1
6,216,593 - BT4738 0.86 -2.4
6,216,670 + BT4738 0.89 -1.7
6,216,702 - -1.5
6,216,707 - -3.1
6,216,713 - -3.8
6,216,713 - -1.1
6,216,716 + -0.8
6,216,937 - -0.7
6,216,941 - -1.0
6,217,312 - -1.2
6,217,320 + -1.7
6,217,389 + -1.7
6,217,390 - -2.4
6,217,475 + -0.7
6,217,501 + -1.1
6,217,509 + -1.1
6,217,510 - -0.2
6,217,517 + -0.1
6,217,735 + BT4739 0.21 +1.5
6,217,736 - BT4739 0.21 -1.3
6,217,832 + BT4739 0.29 -2.9
6,217,832 + BT4739 0.29 -3.1
6,217,838 + BT4739 0.29 -1.7
6,217,890 + BT4739 0.34 -0.1
6,217,891 - BT4739 0.34 +0.9

Or see this region's nucleotide sequence