Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4735 and BT4736 are separated by 213 nucleotides BT4736 and BT4737 overlap by 8 nucleotides BT4737 and BT4738 are separated by 13 nucleotides
BT4735: BT4735 - hypothetical protein (NCBI ptt file), at 6,212,784 to 6,213,287
BT4735
BT4736: BT4736 - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 6,213,501 to 6,213,950
BT4736
BT4737: BT4737 - pyruvate formate-lyase activating enzyme (NCBI ptt file), at 6,213,943 to 6,214,671
BT4737
BT4738: BT4738 - formate acetyltransferase (NCBI ptt file), at 6,214,685 to 6,216,913
BT4738
Position (kb)
6213
6214
6215 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 6213.279 kb on - strand at 6213.322 kb on + strand at 6213.344 kb on - strand at 6213.361 kb on - strand at 6213.443 kb on + strand at 6213.448 kb on + strand at 6213.457 kb on + strand at 6213.621 kb on + strand, within BT4736 at 6213.624 kb on + strand, within BT4736 at 6213.625 kb on - strand, within BT4736 at 6213.625 kb on - strand, within BT4736 at 6213.627 kb on - strand, within BT4736 at 6213.627 kb on - strand, within BT4736 at 6213.669 kb on - strand, within BT4736 at 6213.710 kb on - strand, within BT4736 at 6213.716 kb on - strand, within BT4736 at 6213.716 kb on - strand, within BT4736 at 6213.716 kb on - strand, within BT4736 at 6213.720 kb on + strand, within BT4736 at 6213.764 kb on + strand, within BT4736 at 6213.764 kb on + strand, within BT4736 at 6213.831 kb on - strand, within BT4736 at 6213.836 kb on - strand, within BT4736 at 6213.836 kb on - strand, within BT4736 at 6213.871 kb on - strand, within BT4736 at 6213.884 kb on - strand, within BT4736 at 6213.902 kb on + strand, within BT4736 at 6213.944 kb on + strand at 6213.948 kb on + strand at 6213.960 kb on + strand at 6213.968 kb on + strand at 6214.037 kb on + strand, within BT4737 at 6214.038 kb on - strand, within BT4737 at 6214.062 kb on + strand, within BT4737 at 6214.062 kb on + strand, within BT4737 at 6214.083 kb on - strand, within BT4737 at 6214.167 kb on - strand, within BT4737 at 6214.178 kb on - strand, within BT4737 at 6214.294 kb on + strand, within BT4737 at 6214.297 kb on - strand, within BT4737 at 6214.337 kb on - strand, within BT4737 at 6214.674 kb on - strand at 6214.674 kb on - strand at 6214.674 kb on - strand at 6214.674 kb on - strand at 6214.674 kb on - strand at 6214.691 kb on - strand at 6214.792 kb on - strand at 6214.818 kb on + strand at 6214.820 kb on + strand at 6214.820 kb on + strand at 6214.821 kb on - strand at 6214.825 kb on + strand at 6214.839 kb on - strand at 6215.135 kb on - strand, within BT4738 at 6215.206 kb on - strand, within BT4738 at 6215.232 kb on - strand, within BT4738 at 6215.391 kb on - strand, within BT4738 at 6215.453 kb on - strand, within BT4738 at 6215.454 kb on + strand, within BT4738 at 6215.454 kb on + strand, within BT4738
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 6,213,279 - -0.5 6,213,322 + -1.5 6,213,344 - -0.9 6,213,361 - -1.8 6,213,443 + -1.8 6,213,448 + -2.1 6,213,457 + -0.1 6,213,621 + BT4736 0.27 -1.3 6,213,624 + BT4736 0.27 +2.5 6,213,625 - BT4736 0.28 -1.1 6,213,625 - BT4736 0.28 +1.8 6,213,627 - BT4736 0.28 -1.9 6,213,627 - BT4736 0.28 -0.5 6,213,669 - BT4736 0.37 -1.0 6,213,710 - BT4736 0.46 -1.7 6,213,716 - BT4736 0.48 -0.1 6,213,716 - BT4736 0.48 +1.9 6,213,716 - BT4736 0.48 -0.9 6,213,720 + BT4736 0.49 +0.4 6,213,764 + BT4736 0.58 -0.3 6,213,764 + BT4736 0.58 -2.2 6,213,831 - BT4736 0.73 +1.5 6,213,836 - BT4736 0.74 -1.0 6,213,836 - BT4736 0.74 -1.4 6,213,871 - BT4736 0.82 +1.9 6,213,884 - BT4736 0.85 -0.1 6,213,902 + BT4736 0.89 -2.4 6,213,944 + -1.5 6,213,948 + -3.1 6,213,960 + -1.7 6,213,968 + +2.7 6,214,037 + BT4737 0.13 -4.0 6,214,038 - BT4737 0.13 -3.0 6,214,062 + BT4737 0.16 -0.7 6,214,062 + BT4737 0.16 -0.1 6,214,083 - BT4737 0.19 -4.4 6,214,167 - BT4737 0.31 +0.5 6,214,178 - BT4737 0.32 -4.6 6,214,294 + BT4737 0.48 -2.9 6,214,297 - BT4737 0.49 -0.1 6,214,337 - BT4737 0.54 -1.9 6,214,674 - -0.7 6,214,674 - -4.1 6,214,674 - -1.5 6,214,674 - +0.5 6,214,674 - +0.5 6,214,691 - -2.5 6,214,792 - -4.1 6,214,818 + -3.8 6,214,820 + -3.3 6,214,820 + -1.7 6,214,821 - -2.4 6,214,825 + -5.7 6,214,839 - +0.5 6,215,135 - BT4738 0.20 -3.1 6,215,206 - BT4738 0.23 -2.6 6,215,232 - BT4738 0.25 -2.1 6,215,391 - BT4738 0.32 -0.1 6,215,453 - BT4738 0.34 -3.8 6,215,454 + BT4738 0.34 -2.7 6,215,454 + BT4738 0.34 -2.1
Or see this region's nucleotide sequence