Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4728 and BT4729 are separated by 44 nucleotides BT4729 and BT4730 are separated by 0 nucleotides BT4730 and BT4731 are separated by 156 nucleotides BT4731 and BT4732 are separated by 16 nucleotides BT4732 and BT4733 overlap by 20 nucleotides
BT4728: BT4728 - putative hexose phosphate transport protein (NCBI ptt file), at 6,206,999 to 6,208,357
BT4728
BT4729: BT4729 - hypothetical protein (NCBI ptt file), at 6,208,402 to 6,208,626
BT4729
BT4730: BT4730 - hypothetical protein (NCBI ptt file), at 6,208,627 to 6,208,881
BT4730
BT4731: BT4731 - hypothetical protein (NCBI ptt file), at 6,209,038 to 6,209,454
BT4731
BT4732: BT4732 - conserved hypothetical protein (NCBI ptt file), at 6,209,471 to 6,209,803
BT4732
BT4733: BT4733 - conserved hypothetical protein (NCBI ptt file), at 6,209,784 to 6,210,089
BT4733
Position (kb)
6208
6209 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6207.781 kb on + strand, within BT4728 at 6207.781 kb on + strand, within BT4728 at 6207.782 kb on - strand, within BT4728 at 6207.782 kb on - strand, within BT4728 at 6207.785 kb on + strand, within BT4728 at 6207.785 kb on + strand, within BT4728 at 6207.787 kb on + strand, within BT4728 at 6207.808 kb on + strand, within BT4728 at 6207.845 kb on + strand, within BT4728 at 6207.869 kb on - strand, within BT4728 at 6207.871 kb on + strand, within BT4728 at 6207.873 kb on + strand, within BT4728 at 6207.900 kb on + strand, within BT4728 at 6207.953 kb on - strand, within BT4728 at 6208.008 kb on + strand, within BT4728 at 6208.173 kb on + strand, within BT4728 at 6208.174 kb on - strand, within BT4728 at 6208.263 kb on - strand at 6208.265 kb on - strand at 6208.556 kb on - strand, within BT4729 at 6208.685 kb on + strand, within BT4730 at 6208.716 kb on + strand, within BT4730 at 6208.766 kb on + strand, within BT4730 at 6208.767 kb on - strand, within BT4730 at 6208.775 kb on + strand, within BT4730 at 6208.801 kb on - strand, within BT4730 at 6208.801 kb on - strand, within BT4730 at 6208.803 kb on + strand, within BT4730 at 6208.875 kb on + strand at 6208.879 kb on + strand at 6208.979 kb on - strand at 6208.979 kb on - strand at 6208.979 kb on - strand at 6208.989 kb on - strand at 6209.000 kb on + strand at 6209.045 kb on - strand at 6209.098 kb on - strand, within BT4731 at 6209.150 kb on - strand, within BT4731 at 6209.420 kb on - strand at 6209.487 kb on + strand at 6209.492 kb on + strand at 6209.494 kb on + strand at 6209.555 kb on + strand, within BT4732 at 6209.562 kb on + strand, within BT4732 at 6209.589 kb on + strand, within BT4732 at 6209.602 kb on + strand, within BT4732 at 6209.658 kb on + strand, within BT4732 at 6209.658 kb on + strand, within BT4732 at 6209.660 kb on + strand, within BT4732 at 6209.660 kb on + strand, within BT4732 at 6209.769 kb on + strand, within BT4732 at 6209.783 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 6,207,781 + BT4728 0.58 -0.3 6,207,781 + BT4728 0.58 -0.8 6,207,782 - BT4728 0.58 -1.4 6,207,782 - BT4728 0.58 +0.9 6,207,785 + BT4728 0.58 +1.6 6,207,785 + BT4728 0.58 -1.9 6,207,787 + BT4728 0.58 -2.0 6,207,808 + BT4728 0.60 -0.5 6,207,845 + BT4728 0.62 -1.5 6,207,869 - BT4728 0.64 -0.1 6,207,871 + BT4728 0.64 -1.1 6,207,873 + BT4728 0.64 -0.1 6,207,900 + BT4728 0.66 +0.6 6,207,953 - BT4728 0.70 -1.1 6,208,008 + BT4728 0.74 -1.1 6,208,173 + BT4728 0.86 -1.0 6,208,174 - BT4728 0.86 +1.5 6,208,263 - -0.9 6,208,265 - -1.9 6,208,556 - BT4729 0.68 +0.5 6,208,685 + BT4730 0.23 -1.1 6,208,716 + BT4730 0.35 -2.1 6,208,766 + BT4730 0.55 -2.9 6,208,767 - BT4730 0.55 +2.5 6,208,775 + BT4730 0.58 -2.8 6,208,801 - BT4730 0.68 -3.3 6,208,801 - BT4730 0.68 -2.4 6,208,803 + BT4730 0.69 +3.2 6,208,875 + -0.7 6,208,879 + -1.9 6,208,979 - -3.4 6,208,979 - -1.1 6,208,979 - -2.9 6,208,989 - -1.3 6,209,000 + -1.4 6,209,045 - +0.9 6,209,098 - BT4731 0.14 -2.3 6,209,150 - BT4731 0.27 -2.1 6,209,420 - -1.1 6,209,487 + +3.2 6,209,492 + -2.6 6,209,494 + -4.1 6,209,555 + BT4732 0.25 -0.7 6,209,562 + BT4732 0.27 -1.6 6,209,589 + BT4732 0.35 -0.5 6,209,602 + BT4732 0.39 -1.4 6,209,658 + BT4732 0.56 -4.4 6,209,658 + BT4732 0.56 -1.1 6,209,660 + BT4732 0.57 +1.1 6,209,660 + BT4732 0.57 -3.1 6,209,769 + BT4732 0.89 -2.8 6,209,783 + -1.7
Or see this region's nucleotide sequence