Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4703 and BT4704 overlap by 4 nucleotides BT4704 and BT4705 overlap by 27 nucleotides BT4705 and BT4706 are separated by 224 nucleotides
BT4703: BT4703 - chloromuconate cycloisomerase (NCBI ptt file), at 6,172,849 to 6,173,874
BT4703
BT4704: BT4704 - O-succinylbenzoic acid--CoA ligase (NCBI ptt file), at 6,173,871 to 6,174,977
BT4704
BT4705: BT4705 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 6,174,951 to 6,175,520
BT4705
BT4706: BT4706 - putative anti-sigma factor (NCBI ptt file), at 6,175,745 to 6,176,668
BT4706
Position (kb)
6173
6174
6175 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6173.053 kb on - strand, within BT4703 at 6173.184 kb on + strand, within BT4703 at 6173.211 kb on + strand, within BT4703 at 6173.385 kb on - strand, within BT4703 at 6173.465 kb on + strand, within BT4703 at 6173.521 kb on - strand, within BT4703 at 6173.565 kb on - strand, within BT4703 at 6173.570 kb on - strand, within BT4703 at 6173.571 kb on + strand, within BT4703 at 6173.574 kb on + strand, within BT4703 at 6173.575 kb on - strand, within BT4703 at 6173.626 kb on - strand, within BT4703 at 6173.628 kb on + strand, within BT4703 at 6173.711 kb on + strand, within BT4703 at 6173.807 kb on + strand at 6173.820 kb on + strand at 6173.825 kb on + strand at 6173.922 kb on + strand at 6174.068 kb on + strand, within BT4704 at 6174.158 kb on - strand, within BT4704 at 6174.178 kb on - strand, within BT4704 at 6174.233 kb on - strand, within BT4704 at 6174.235 kb on + strand, within BT4704 at 6174.236 kb on - strand, within BT4704 at 6174.313 kb on - strand, within BT4704 at 6174.313 kb on - strand, within BT4704 at 6174.317 kb on + strand, within BT4704 at 6174.318 kb on - strand, within BT4704 at 6174.318 kb on - strand, within BT4704 at 6174.371 kb on + strand, within BT4704 at 6174.513 kb on + strand, within BT4704 at 6174.517 kb on - strand, within BT4704 at 6174.569 kb on + strand, within BT4704 at 6174.570 kb on - strand, within BT4704 at 6174.674 kb on + strand, within BT4704 at 6174.675 kb on - strand, within BT4704 at 6174.687 kb on - strand, within BT4704 at 6174.815 kb on - strand, within BT4704 at 6174.831 kb on - strand, within BT4704 at 6174.855 kb on + strand, within BT4704 at 6174.856 kb on - strand, within BT4704 at 6174.859 kb on - strand, within BT4704 at 6174.873 kb on - strand at 6174.873 kb on - strand at 6174.917 kb on + strand at 6174.940 kb on + strand at 6174.952 kb on + strand at 6175.040 kb on - strand, within BT4705 at 6175.040 kb on - strand, within BT4705 at 6175.047 kb on - strand, within BT4705 at 6175.119 kb on - strand, within BT4705 at 6175.148 kb on + strand, within BT4705 at 6175.177 kb on - strand, within BT4705 at 6175.191 kb on - strand, within BT4705 at 6175.194 kb on + strand, within BT4705 at 6175.194 kb on + strand, within BT4705 at 6175.195 kb on - strand, within BT4705 at 6175.195 kb on - strand, within BT4705 at 6175.250 kb on - strand, within BT4705 at 6175.311 kb on + strand, within BT4705 at 6175.311 kb on + strand, within BT4705 at 6175.312 kb on - strand, within BT4705 at 6175.312 kb on - strand, within BT4705 at 6175.384 kb on - strand, within BT4705 at 6175.384 kb on - strand, within BT4705 at 6175.559 kb on - strand at 6175.565 kb on + strand at 6175.636 kb on - strand at 6175.667 kb on + strand at 6175.701 kb on + strand at 6175.727 kb on - strand at 6175.791 kb on + strand at 6175.819 kb on + strand at 6175.828 kb on - strand at 6175.882 kb on + strand, within BT4706 at 6175.883 kb on - strand, within BT4706 at 6175.883 kb on - strand, within BT4706 at 6175.889 kb on - strand, within BT4706 at 6175.889 kb on - strand, within BT4706 at 6175.923 kb on + strand, within BT4706 at 6175.923 kb on + strand, within BT4706 at 6175.924 kb on - strand, within BT4706 at 6175.960 kb on - strand, within BT4706 at 6175.960 kb on - strand, within BT4706
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 6,173,053 - BT4703 0.20 +0.9 6,173,184 + BT4703 0.33 -3.8 6,173,211 + BT4703 0.35 -3.3 6,173,385 - BT4703 0.52 -1.5 6,173,465 + BT4703 0.60 -4.2 6,173,521 - BT4703 0.65 -1.9 6,173,565 - BT4703 0.70 +2.2 6,173,570 - BT4703 0.70 -1.1 6,173,571 + BT4703 0.70 +0.3 6,173,574 + BT4703 0.71 +0.2 6,173,575 - BT4703 0.71 -2.6 6,173,626 - BT4703 0.76 +0.6 6,173,628 + BT4703 0.76 -0.1 6,173,711 + BT4703 0.84 -1.7 6,173,807 + -1.1 6,173,820 + +1.5 6,173,825 + -1.7 6,173,922 + -0.1 6,174,068 + BT4704 0.18 -1.4 6,174,158 - BT4704 0.26 -3.6 6,174,178 - BT4704 0.28 +0.1 6,174,233 - BT4704 0.33 -1.1 6,174,235 + BT4704 0.33 -0.1 6,174,236 - BT4704 0.33 -1.7 6,174,313 - BT4704 0.40 -2.1 6,174,313 - BT4704 0.40 +0.3 6,174,317 + BT4704 0.40 -2.1 6,174,318 - BT4704 0.40 -1.7 6,174,318 - BT4704 0.40 -1.1 6,174,371 + BT4704 0.45 -1.3 6,174,513 + BT4704 0.58 -3.1 6,174,517 - BT4704 0.58 -1.7 6,174,569 + BT4704 0.63 -0.7 6,174,570 - BT4704 0.63 -3.8 6,174,674 + BT4704 0.73 -1.7 6,174,675 - BT4704 0.73 -0.1 6,174,687 - BT4704 0.74 +1.5 6,174,815 - BT4704 0.85 -1.1 6,174,831 - BT4704 0.87 +0.9 6,174,855 + BT4704 0.89 -1.8 6,174,856 - BT4704 0.89 -2.1 6,174,859 - BT4704 0.89 -2.7 6,174,873 - -1.7 6,174,873 - +1.2 6,174,917 + -1.7 6,174,940 + -1.1 6,174,952 + -0.4 6,175,040 - BT4705 0.16 +2.3 6,175,040 - BT4705 0.16 -1.3 6,175,047 - BT4705 0.17 -0.7 6,175,119 - BT4705 0.29 +0.1 6,175,148 + BT4705 0.35 -0.7 6,175,177 - BT4705 0.40 -2.9 6,175,191 - BT4705 0.42 +0.9 6,175,194 + BT4705 0.43 -2.0 6,175,194 + BT4705 0.43 -3.1 6,175,195 - BT4705 0.43 -1.5 6,175,195 - BT4705 0.43 -0.5 6,175,250 - BT4705 0.52 -2.1 6,175,311 + BT4705 0.63 +0.5 6,175,311 + BT4705 0.63 -1.1 6,175,312 - BT4705 0.63 -1.6 6,175,312 - BT4705 0.63 +0.6 6,175,384 - BT4705 0.76 -1.3 6,175,384 - BT4705 0.76 -1.1 6,175,559 - -1.3 6,175,565 + -1.1 6,175,636 - -1.5 6,175,667 + -1.1 6,175,701 + -2.1 6,175,727 - +2.4 6,175,791 + -1.8 6,175,819 + -3.7 6,175,828 - -1.5 6,175,882 + BT4706 0.15 +3.1 6,175,883 - BT4706 0.15 +0.8 6,175,883 - BT4706 0.15 +0.9 6,175,889 - BT4706 0.16 +2.7 6,175,889 - BT4706 0.16 -2.8 6,175,923 + BT4706 0.19 -3.9 6,175,923 + BT4706 0.19 +1.9 6,175,924 - BT4706 0.19 -2.1 6,175,960 - BT4706 0.23 -2.1 6,175,960 - BT4706 0.23 +0.2
Or see this region's nucleotide sequence