Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4583 and BT4584 are separated by 49 nucleotides BT4584 and BT4585 are separated by 150 nucleotides
BT4583: BT4583 - peptidase T (tripeptide aminopeptidase) (NCBI ptt file), at 6,005,103 to 6,006,326
BT4583
BT4584: BT4584 - putative aminomethyltransferase (NCBI ptt file), at 6,006,376 to 6,007,461
BT4584
BT4585: BT4585 - putative Na+/H+ anitporter (NCBI ptt file), at 6,007,612 to 6,009,885
BT4585
Position (kb)
6006
6007
6008 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6005.378 kb on + strand, within BT4583 at 6005.391 kb on + strand, within BT4583 at 6005.392 kb on - strand, within BT4583 at 6005.392 kb on - strand, within BT4583 at 6005.406 kb on - strand, within BT4583 at 6005.471 kb on + strand, within BT4583 at 6005.471 kb on + strand, within BT4583 at 6005.526 kb on - strand, within BT4583 at 6005.766 kb on - strand, within BT4583 at 6005.766 kb on - strand, within BT4583 at 6005.793 kb on - strand, within BT4583 at 6005.801 kb on - strand, within BT4583 at 6005.875 kb on - strand, within BT4583 at 6005.934 kb on + strand, within BT4583 at 6005.935 kb on - strand, within BT4583 at 6005.935 kb on - strand, within BT4583 at 6005.936 kb on + strand, within BT4583 at 6005.936 kb on + strand, within BT4583 at 6006.035 kb on + strand, within BT4583 at 6006.060 kb on + strand, within BT4583 at 6006.060 kb on + strand, within BT4583 at 6006.065 kb on + strand, within BT4583 at 6006.066 kb on - strand, within BT4583 at 6006.314 kb on - strand at 6006.413 kb on + strand at 6006.413 kb on + strand at 6006.413 kb on + strand at 6006.420 kb on + strand at 6006.442 kb on + strand at 6006.444 kb on + strand at 6006.444 kb on + strand at 6006.444 kb on + strand at 6006.445 kb on - strand at 6006.447 kb on + strand at 6006.447 kb on + strand at 6006.495 kb on + strand, within BT4584 at 6006.510 kb on - strand, within BT4584 at 6006.623 kb on - strand, within BT4584 at 6006.624 kb on + strand, within BT4584 at 6006.624 kb on + strand, within BT4584 at 6006.625 kb on - strand, within BT4584 at 6006.674 kb on - strand, within BT4584 at 6006.722 kb on + strand, within BT4584 at 6006.723 kb on - strand, within BT4584 at 6006.792 kb on + strand, within BT4584 at 6006.792 kb on + strand, within BT4584 at 6006.826 kb on - strand, within BT4584 at 6006.850 kb on + strand, within BT4584 at 6006.880 kb on - strand, within BT4584 at 6006.880 kb on - strand, within BT4584 at 6006.940 kb on - strand, within BT4584 at 6007.090 kb on + strand, within BT4584 at 6007.090 kb on + strand, within BT4584 at 6007.091 kb on - strand, within BT4584 at 6007.226 kb on + strand, within BT4584 at 6007.361 kb on - strand at 6007.361 kb on - strand at 6007.408 kb on - strand at 6007.566 kb on + strand at 6007.584 kb on + strand at 6007.585 kb on - strand at 6007.601 kb on - strand at 6007.623 kb on - strand at 6007.623 kb on - strand at 6007.627 kb on - strand at 6007.653 kb on - strand at 6007.758 kb on + strand at 6007.759 kb on - strand at 6007.759 kb on - strand at 6007.759 kb on - strand at 6007.882 kb on - strand, within BT4585 at 6007.929 kb on - strand, within BT4585 at 6007.977 kb on - strand, within BT4585 at 6008.002 kb on - strand, within BT4585 at 6008.002 kb on - strand, within BT4585 at 6008.010 kb on + strand, within BT4585 at 6008.011 kb on - strand, within BT4585 at 6008.092 kb on - strand, within BT4585 at 6008.143 kb on - strand, within BT4585 at 6008.173 kb on - strand, within BT4585 at 6008.196 kb on + strand, within BT4585 at 6008.211 kb on - strand, within BT4585 at 6008.213 kb on + strand, within BT4585 at 6008.286 kb on - strand, within BT4585 at 6008.400 kb on + strand, within BT4585
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 6,005,378 + BT4583 0.22 -3.4 6,005,391 + BT4583 0.24 -0.1 6,005,392 - BT4583 0.24 -0.4 6,005,392 - BT4583 0.24 +0.5 6,005,406 - BT4583 0.25 -0.2 6,005,471 + BT4583 0.30 +2.2 6,005,471 + BT4583 0.30 -0.7 6,005,526 - BT4583 0.35 -0.4 6,005,766 - BT4583 0.54 +1.9 6,005,766 - BT4583 0.54 -0.1 6,005,793 - BT4583 0.56 -2.7 6,005,801 - BT4583 0.57 -0.9 6,005,875 - BT4583 0.63 +0.7 6,005,934 + BT4583 0.68 -1.7 6,005,935 - BT4583 0.68 +2.4 6,005,935 - BT4583 0.68 +2.5 6,005,936 + BT4583 0.68 +1.2 6,005,936 + BT4583 0.68 -0.1 6,006,035 + BT4583 0.76 -0.8 6,006,060 + BT4583 0.78 -2.1 6,006,060 + BT4583 0.78 -1.4 6,006,065 + BT4583 0.79 +0.9 6,006,066 - BT4583 0.79 -1.3 6,006,314 - -0.8 6,006,413 + +2.1 6,006,413 + -0.4 6,006,413 + -1.7 6,006,420 + +0.5 6,006,442 + -1.3 6,006,444 + +1.1 6,006,444 + -2.4 6,006,444 + -0.1 6,006,445 - +2.6 6,006,447 + -0.7 6,006,447 + +1.5 6,006,495 + BT4584 0.11 -1.9 6,006,510 - BT4584 0.12 +2.5 6,006,623 - BT4584 0.23 -1.0 6,006,624 + BT4584 0.23 -0.4 6,006,624 + BT4584 0.23 +3.6 6,006,625 - BT4584 0.23 +0.5 6,006,674 - BT4584 0.27 -1.6 6,006,722 + BT4584 0.32 -1.9 6,006,723 - BT4584 0.32 +2.5 6,006,792 + BT4584 0.38 -0.4 6,006,792 + BT4584 0.38 -3.3 6,006,826 - BT4584 0.41 -0.7 6,006,850 + BT4584 0.44 -2.4 6,006,880 - BT4584 0.46 +0.2 6,006,880 - BT4584 0.46 +1.3 6,006,940 - BT4584 0.52 -1.1 6,007,090 + BT4584 0.66 +1.5 6,007,090 + BT4584 0.66 +1.3 6,007,091 - BT4584 0.66 -1.4 6,007,226 + BT4584 0.78 +1.3 6,007,361 - +0.9 6,007,361 - -1.9 6,007,408 - -2.9 6,007,566 + +2.2 6,007,584 + -0.4 6,007,585 - +3.5 6,007,601 - -4.3 6,007,623 - -0.1 6,007,623 - -2.0 6,007,627 - -1.1 6,007,653 - -0.8 6,007,758 + +1.5 6,007,759 - -2.5 6,007,759 - +3.3 6,007,759 - +1.5 6,007,882 - BT4585 0.12 +1.2 6,007,929 - BT4585 0.14 -1.0 6,007,977 - BT4585 0.16 -1.1 6,008,002 - BT4585 0.17 -0.1 6,008,002 - BT4585 0.17 +2.5 6,008,010 + BT4585 0.18 +2.0 6,008,011 - BT4585 0.18 -0.4 6,008,092 - BT4585 0.21 -2.4 6,008,143 - BT4585 0.23 +1.7 6,008,173 - BT4585 0.25 -1.8 6,008,196 + BT4585 0.26 -3.3 6,008,211 - BT4585 0.26 -1.9 6,008,213 + BT4585 0.26 -0.4 6,008,286 - BT4585 0.30 -2.7 6,008,400 + BT4585 0.35 +2.0
Or see this region's nucleotide sequence