Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4531 and BT4532 are separated by 68 nucleotides BT4532 and BT4533 are separated by 479 nucleotides BT4533 and BT4534 are separated by 141 nucleotides BT4534 and BT4535 are separated by 20 nucleotides
BT4531: BT4531 - conserved hypothetical protein (NCBI ptt file), at 5,948,861 to 5,949,916
BT4531
BT4532: BT4532 - hypothetical protein (NCBI ptt file), at 5,949,985 to 5,950,302
BT4532
BT4533: BT4533 - conserved hypothetical protein (NCBI ptt file), at 5,950,782 to 5,952,131
BT4533
BT4534: BT4534 - hypothetical protein (NCBI ptt file), at 5,952,273 to 5,952,557
BT4534
BT4535: BT4535 - Type I restriction enzyme EcoR124II R protein (NCBI ptt file), at 5,952,578 to 5,955,406
BT4535
Position (kb)
5950
5951
5952
5953 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 5949.853 kb on - strand at 5949.914 kb on + strand at 5949.921 kb on - strand at 5949.956 kb on + strand at 5949.957 kb on - strand at 5949.957 kb on - strand at 5949.960 kb on + strand at 5949.960 kb on + strand at 5949.961 kb on - strand at 5949.962 kb on + strand at 5949.972 kb on - strand at 5949.973 kb on + strand at 5950.010 kb on - strand at 5950.010 kb on - strand at 5950.015 kb on + strand at 5950.054 kb on - strand, within BT4532 at 5950.054 kb on - strand, within BT4532 at 5950.058 kb on - strand, within BT4532 at 5950.058 kb on - strand, within BT4532 at 5950.119 kb on - strand, within BT4532 at 5950.165 kb on - strand, within BT4532 at 5950.202 kb on + strand, within BT4532 at 5950.202 kb on + strand, within BT4532 at 5950.203 kb on - strand, within BT4532 at 5950.218 kb on - strand, within BT4532 at 5950.222 kb on - strand, within BT4532 at 5950.230 kb on - strand, within BT4532 at 5950.257 kb on + strand, within BT4532 at 5950.283 kb on - strand at 5950.283 kb on - strand at 5950.287 kb on + strand at 5950.330 kb on - strand at 5950.330 kb on - strand at 5950.332 kb on - strand at 5950.363 kb on + strand at 5950.363 kb on + strand at 5950.397 kb on + strand at 5950.397 kb on + strand at 5950.398 kb on - strand at 5950.439 kb on - strand at 5950.462 kb on + strand at 5950.463 kb on - strand at 5950.552 kb on + strand at 5950.552 kb on + strand at 5950.553 kb on - strand at 5950.588 kb on - strand at 5950.590 kb on + strand at 5950.609 kb on + strand at 5950.617 kb on + strand at 5950.617 kb on + strand at 5950.647 kb on - strand at 5950.716 kb on + strand at 5950.754 kb on - strand at 5950.781 kb on + strand at 5950.782 kb on - strand at 5950.786 kb on + strand at 5950.826 kb on - strand at 5950.872 kb on - strand at 5950.948 kb on + strand, within BT4533 at 5951.027 kb on + strand, within BT4533 at 5951.027 kb on + strand, within BT4533 at 5951.107 kb on - strand, within BT4533 at 5951.111 kb on + strand, within BT4533 at 5951.145 kb on + strand, within BT4533 at 5951.177 kb on + strand, within BT4533 at 5951.278 kb on + strand, within BT4533 at 5951.278 kb on + strand, within BT4533 at 5951.343 kb on + strand, within BT4533 at 5951.344 kb on - strand, within BT4533 at 5951.367 kb on + strand, within BT4533 at 5951.379 kb on + strand, within BT4533 at 5951.460 kb on + strand, within BT4533 at 5951.477 kb on + strand, within BT4533 at 5951.494 kb on + strand, within BT4533 at 5951.516 kb on - strand, within BT4533 at 5951.597 kb on + strand, within BT4533 at 5951.704 kb on + strand, within BT4533 at 5951.828 kb on + strand, within BT4533 at 5951.837 kb on + strand, within BT4533 at 5951.871 kb on + strand, within BT4533 at 5951.940 kb on - strand, within BT4533 at 5951.976 kb on - strand, within BT4533 at 5951.976 kb on - strand, within BT4533 at 5952.034 kb on + strand at 5952.063 kb on - strand at 5952.159 kb on - strand at 5952.220 kb on - strand at 5952.224 kb on - strand at 5952.224 kb on - strand at 5952.224 kb on - strand at 5952.269 kb on + strand at 5952.269 kb on + strand at 5952.270 kb on - strand at 5952.270 kb on - strand at 5952.320 kb on - strand, within BT4534 at 5952.363 kb on - strand, within BT4534 at 5952.364 kb on + strand, within BT4534 at 5952.374 kb on + strand, within BT4534 at 5952.464 kb on - strand, within BT4534 at 5952.480 kb on - strand, within BT4534 at 5952.480 kb on - strand, within BT4534 at 5952.480 kb on - strand, within BT4534 at 5952.544 kb on + strand at 5952.545 kb on - strand at 5952.555 kb on + strand at 5952.578 kb on - strand at 5952.622 kb on + strand at 5952.622 kb on + strand at 5952.623 kb on - strand at 5952.641 kb on + strand at 5952.648 kb on - strand at 5952.678 kb on - strand at 5952.740 kb on - strand at 5952.740 kb on - strand at 5952.779 kb on + strand at 5952.859 kb on + strand at 5952.859 kb on + strand at 5952.860 kb on - strand at 5952.860 kb on - strand at 5952.860 kb on - strand at 5952.860 kb on - strand at 5952.862 kb on - strand, within BT4535 at 5952.877 kb on - strand, within BT4535 at 5953.034 kb on + strand, within BT4535 at 5953.034 kb on + strand, within BT4535 at 5953.034 kb on + strand, within BT4535 at 5953.044 kb on + strand, within BT4535 at 5953.085 kb on + strand, within BT4535 at 5953.120 kb on + strand, within BT4535 at 5953.120 kb on + strand, within BT4535 at 5953.127 kb on + strand, within BT4535
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 5,949,853 - +3.1 5,949,914 + +2.6 5,949,921 - +2.0 5,949,956 + +1.4 5,949,957 - +0.5 5,949,957 - -1.1 5,949,960 + +2.2 5,949,960 + +0.6 5,949,961 - +2.6 5,949,962 + +3.6 5,949,972 - +1.6 5,949,973 + +2.2 5,950,010 - +1.9 5,950,010 - +4.9 5,950,015 + +1.7 5,950,054 - BT4532 0.22 +2.5 5,950,054 - BT4532 0.22 +6.5 5,950,058 - BT4532 0.23 +0.7 5,950,058 - BT4532 0.23 +4.0 5,950,119 - BT4532 0.42 +4.4 5,950,165 - BT4532 0.57 +2.2 5,950,202 + BT4532 0.68 +0.9 5,950,202 + BT4532 0.68 +1.2 5,950,203 - BT4532 0.69 +3.5 5,950,218 - BT4532 0.73 +1.5 5,950,222 - BT4532 0.75 +4.4 5,950,230 - BT4532 0.77 +0.2 5,950,257 + BT4532 0.86 -0.1 5,950,283 - +0.7 5,950,283 - +2.9 5,950,287 + +0.1 5,950,330 - +2.3 5,950,330 - +3.8 5,950,332 - +3.2 5,950,363 + +0.2 5,950,363 + +4.9 5,950,397 + +1.5 5,950,397 + +2.5 5,950,398 - +3.5 5,950,439 - +4.0 5,950,462 + +2.2 5,950,463 - +4.0 5,950,552 + +2.4 5,950,552 + +3.4 5,950,553 - +3.1 5,950,588 - +2.7 5,950,590 + +2.1 5,950,609 + +0.6 5,950,617 + +1.5 5,950,617 + +2.6 5,950,647 - +0.0 5,950,716 + +0.7 5,950,754 - +0.7 5,950,781 + +1.4 5,950,782 - +1.7 5,950,786 + +2.1 5,950,826 - +1.3 5,950,872 - +0.4 5,950,948 + BT4533 0.12 +3.9 5,951,027 + BT4533 0.18 +0.4 5,951,027 + BT4533 0.18 +5.2 5,951,107 - BT4533 0.24 +2.0 5,951,111 + BT4533 0.24 +1.1 5,951,145 + BT4533 0.27 +2.6 5,951,177 + BT4533 0.29 +4.4 5,951,278 + BT4533 0.37 +2.7 5,951,278 + BT4533 0.37 +2.7 5,951,343 + BT4533 0.42 +2.9 5,951,344 - BT4533 0.42 +1.8 5,951,367 + BT4533 0.43 +1.2 5,951,379 + BT4533 0.44 +2.7 5,951,460 + BT4533 0.50 +2.3 5,951,477 + BT4533 0.51 +2.7 5,951,494 + BT4533 0.53 +2.8 5,951,516 - BT4533 0.54 +5.4 5,951,597 + BT4533 0.60 +0.7 5,951,704 + BT4533 0.68 +4.1 5,951,828 + BT4533 0.77 +2.1 5,951,837 + BT4533 0.78 +2.0 5,951,871 + BT4533 0.81 +2.5 5,951,940 - BT4533 0.86 +2.2 5,951,976 - BT4533 0.88 -1.1 5,951,976 - BT4533 0.88 +2.9 5,952,034 + +1.0 5,952,063 - +2.5 5,952,159 - -0.8 5,952,220 - -2.9 5,952,224 - -0.4 5,952,224 - -1.1 5,952,224 - -1.2 5,952,269 + -3.4 5,952,269 + -0.1 5,952,270 - +2.7 5,952,270 - -0.9 5,952,320 - BT4534 0.16 -2.6 5,952,363 - BT4534 0.32 +2.7 5,952,364 + BT4534 0.32 +0.5 5,952,374 + BT4534 0.35 -1.7 5,952,464 - BT4534 0.67 -2.3 5,952,480 - BT4534 0.73 -2.7 5,952,480 - BT4534 0.73 +1.5 5,952,480 - BT4534 0.73 -2.1 5,952,544 + -1.9 5,952,545 - -0.5 5,952,555 + -1.1 5,952,578 - +3.7 5,952,622 + +2.7 5,952,622 + +0.2 5,952,623 - +2.6 5,952,641 + +1.7 5,952,648 - -2.8 5,952,678 - -2.4 5,952,740 - +1.1 5,952,740 - -2.1 5,952,779 + -0.3 5,952,859 + +0.5 5,952,859 + -1.7 5,952,860 - -1.1 5,952,860 - +2.5 5,952,860 - -1.1 5,952,860 - -1.0 5,952,862 - BT4535 0.10 +1.6 5,952,877 - BT4535 0.11 +0.5 5,953,034 + BT4535 0.16 +1.1 5,953,034 + BT4535 0.16 +0.9 5,953,034 + BT4535 0.16 -1.1 5,953,044 + BT4535 0.16 -4.6 5,953,085 + BT4535 0.18 -2.1 5,953,120 + BT4535 0.19 +2.7 5,953,120 + BT4535 0.19 -2.1 5,953,127 + BT4535 0.19 +1.7
Or see this region's nucleotide sequence