Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4528 and BT4529 are separated by 441 nucleotides BT4529 and BT4530 are separated by 5 nucleotides BT4530 and BT4531 are separated by 146 nucleotides
BT4528: BT4528 - conserved hypothetical protein (NCBI ptt file), at 5,945,992 to 5,946,576
BT4528
BT4529: BT4529 - hypothetical protein (NCBI ptt file), at 5,947,018 to 5,947,287
BT4529
BT4530: BT4530 - ATP-dependent DNA helicase recG (NCBI ptt file), at 5,947,293 to 5,948,714
BT4530
BT4531: BT4531 - conserved hypothetical protein (NCBI ptt file), at 5,948,861 to 5,949,916
BT4531
Position (kb)
5947
5948
5949 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 5946.302 kb on - strand, within BT4528 at 5946.304 kb on - strand, within BT4528 at 5946.327 kb on + strand, within BT4528 at 5946.436 kb on - strand, within BT4528 at 5946.454 kb on + strand, within BT4528 at 5946.456 kb on + strand, within BT4528 at 5946.483 kb on + strand, within BT4528 at 5946.541 kb on - strand at 5946.553 kb on - strand at 5946.568 kb on - strand at 5946.749 kb on - strand at 5946.749 kb on - strand at 5946.756 kb on + strand at 5946.756 kb on + strand at 5946.757 kb on - strand at 5946.769 kb on - strand at 5946.875 kb on + strand at 5946.876 kb on - strand at 5946.991 kb on + strand at 5946.992 kb on - strand at 5946.996 kb on + strand at 5946.998 kb on + strand at 5946.999 kb on - strand at 5947.034 kb on + strand at 5947.098 kb on + strand, within BT4529 at 5947.099 kb on - strand, within BT4529 at 5947.101 kb on - strand, within BT4529 at 5947.109 kb on - strand, within BT4529 at 5947.113 kb on - strand, within BT4529 at 5947.297 kb on - strand at 5947.315 kb on + strand at 5947.316 kb on - strand at 5947.350 kb on - strand at 5947.385 kb on + strand at 5947.386 kb on - strand at 5947.434 kb on - strand at 5947.442 kb on + strand, within BT4530 at 5947.548 kb on + strand, within BT4530 at 5947.549 kb on - strand, within BT4530 at 5947.560 kb on - strand, within BT4530 at 5947.657 kb on + strand, within BT4530 at 5947.703 kb on + strand, within BT4530 at 5947.707 kb on + strand, within BT4530 at 5947.726 kb on - strand, within BT4530 at 5947.831 kb on - strand, within BT4530 at 5947.860 kb on + strand, within BT4530 at 5947.860 kb on + strand, within BT4530 at 5947.895 kb on + strand, within BT4530 at 5947.898 kb on - strand, within BT4530 at 5947.953 kb on - strand, within BT4530 at 5948.059 kb on - strand, within BT4530 at 5948.089 kb on - strand, within BT4530 at 5948.140 kb on + strand, within BT4530 at 5948.187 kb on - strand, within BT4530 at 5948.190 kb on - strand, within BT4530 at 5948.279 kb on + strand, within BT4530 at 5948.280 kb on - strand, within BT4530 at 5948.334 kb on + strand, within BT4530 at 5948.334 kb on + strand, within BT4530 at 5948.334 kb on + strand, within BT4530 at 5948.341 kb on - strand, within BT4530 at 5948.341 kb on - strand, within BT4530 at 5948.341 kb on - strand, within BT4530 at 5948.365 kb on + strand, within BT4530 at 5948.373 kb on - strand, within BT4530 at 5948.433 kb on - strand, within BT4530 at 5948.728 kb on - strand at 5948.728 kb on - strand at 5948.728 kb on - strand at 5948.752 kb on - strand at 5948.757 kb on + strand at 5948.916 kb on - strand at 5948.916 kb on - strand at 5948.918 kb on - strand at 5948.921 kb on - strand at 5948.937 kb on - strand at 5948.937 kb on - strand at 5948.937 kb on - strand at 5948.963 kb on + strand at 5948.990 kb on - strand, within BT4531 at 5949.047 kb on + strand, within BT4531 at 5949.048 kb on - strand, within BT4531 at 5949.048 kb on - strand, within BT4531 at 5949.048 kb on - strand, within BT4531 at 5949.076 kb on - strand, within BT4531 at 5949.150 kb on - strand, within BT4531 at 5949.158 kb on - strand, within BT4531 at 5949.158 kb on - strand, within BT4531 at 5949.160 kb on + strand, within BT4531 at 5949.211 kb on + strand, within BT4531 at 5949.239 kb on - strand, within BT4531 at 5949.311 kb on - strand, within BT4531 at 5949.347 kb on - strand, within BT4531 at 5949.461 kb on - strand, within BT4531 at 5949.481 kb on - strand, within BT4531 at 5949.481 kb on - strand, within BT4531 at 5949.513 kb on - strand, within BT4531 at 5949.527 kb on - strand, within BT4531 at 5949.706 kb on - strand, within BT4531
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 5,946,302 - BT4528 0.53 -1.9 5,946,304 - BT4528 0.53 -1.9 5,946,327 + BT4528 0.57 -1.7 5,946,436 - BT4528 0.76 -2.4 5,946,454 + BT4528 0.79 -2.9 5,946,456 + BT4528 0.79 -1.2 5,946,483 + BT4528 0.84 -1.6 5,946,541 - +0.9 5,946,553 - -1.1 5,946,568 - -0.1 5,946,749 - -2.8 5,946,749 - +2.2 5,946,756 + -2.3 5,946,756 + -2.5 5,946,757 - +0.5 5,946,769 - -1.1 5,946,875 + +2.5 5,946,876 - -1.4 5,946,991 + -0.1 5,946,992 - -1.6 5,946,996 + +0.9 5,946,998 + +2.7 5,946,999 - -2.3 5,947,034 + +2.5 5,947,098 + BT4529 0.30 -1.1 5,947,099 - BT4529 0.30 +1.0 5,947,101 - BT4529 0.31 +1.8 5,947,109 - BT4529 0.34 +4.9 5,947,113 - BT4529 0.35 +1.0 5,947,297 - +0.9 5,947,315 + -0.2 5,947,316 - +2.0 5,947,350 - +3.8 5,947,385 + -1.9 5,947,386 - +2.1 5,947,434 - -0.1 5,947,442 + BT4530 0.10 -1.7 5,947,548 + BT4530 0.18 -0.1 5,947,549 - BT4530 0.18 +4.3 5,947,560 - BT4530 0.19 +3.7 5,947,657 + BT4530 0.26 -1.1 5,947,703 + BT4530 0.29 -3.7 5,947,707 + BT4530 0.29 -2.0 5,947,726 - BT4530 0.30 +1.7 5,947,831 - BT4530 0.38 +4.1 5,947,860 + BT4530 0.40 -2.4 5,947,860 + BT4530 0.40 -2.1 5,947,895 + BT4530 0.42 -0.8 5,947,898 - BT4530 0.43 +3.4 5,947,953 - BT4530 0.46 +3.6 5,948,059 - BT4530 0.54 +1.8 5,948,089 - BT4530 0.56 +2.9 5,948,140 + BT4530 0.60 +0.9 5,948,187 - BT4530 0.63 +4.3 5,948,190 - BT4530 0.63 +3.2 5,948,279 + BT4530 0.69 -2.6 5,948,280 - BT4530 0.69 +2.3 5,948,334 + BT4530 0.73 -0.1 5,948,334 + BT4530 0.73 -0.1 5,948,334 + BT4530 0.73 -1.0 5,948,341 - BT4530 0.74 +0.1 5,948,341 - BT4530 0.74 +1.3 5,948,341 - BT4530 0.74 +1.3 5,948,365 + BT4530 0.75 -3.1 5,948,373 - BT4530 0.76 +1.6 5,948,433 - BT4530 0.80 +2.2 5,948,728 - +0.6 5,948,728 - +4.6 5,948,728 - +3.2 5,948,752 - +2.2 5,948,757 + -1.1 5,948,916 - +2.7 5,948,916 - +3.7 5,948,918 - +1.7 5,948,921 - +3.6 5,948,937 - +2.2 5,948,937 - +2.5 5,948,937 - +1.8 5,948,963 + +3.5 5,948,990 - BT4531 0.12 +5.1 5,949,047 + BT4531 0.18 +2.6 5,949,048 - BT4531 0.18 +1.3 5,949,048 - BT4531 0.18 +3.6 5,949,048 - BT4531 0.18 +1.7 5,949,076 - BT4531 0.20 +1.1 5,949,150 - BT4531 0.27 +1.4 5,949,158 - BT4531 0.28 +1.4 5,949,158 - BT4531 0.28 +4.2 5,949,160 + BT4531 0.28 -0.1 5,949,211 + BT4531 0.33 -0.7 5,949,239 - BT4531 0.36 +3.6 5,949,311 - BT4531 0.43 +0.9 5,949,347 - BT4531 0.46 +2.1 5,949,461 - BT4531 0.57 +2.8 5,949,481 - BT4531 0.59 +4.4 5,949,481 - BT4531 0.59 +1.7 5,949,513 - BT4531 0.62 +2.6 5,949,527 - BT4531 0.63 +1.6 5,949,706 - BT4531 0.80 +0.8
Or see this region's nucleotide sequence