Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4530

Experiment: BHIS with Trimethoprim 0.00625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4528 and BT4529 are separated by 441 nucleotidesBT4529 and BT4530 are separated by 5 nucleotidesBT4530 and BT4531 are separated by 146 nucleotides BT4528: BT4528 - conserved hypothetical protein (NCBI ptt file), at 5,945,992 to 5,946,576 BT4528 BT4529: BT4529 - hypothetical protein (NCBI ptt file), at 5,947,018 to 5,947,287 BT4529 BT4530: BT4530 - ATP-dependent DNA helicase recG (NCBI ptt file), at 5,947,293 to 5,948,714 BT4530 BT4531: BT4531 - conserved hypothetical protein (NCBI ptt file), at 5,948,861 to 5,949,916 BT4531 Position (kb) 5947 5948 5949Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 5946.302 kb on - strand, within BT4528at 5946.304 kb on - strand, within BT4528at 5946.327 kb on + strand, within BT4528at 5946.436 kb on - strand, within BT4528at 5946.454 kb on + strand, within BT4528at 5946.456 kb on + strand, within BT4528at 5946.483 kb on + strand, within BT4528at 5946.541 kb on - strandat 5946.553 kb on - strandat 5946.568 kb on - strandat 5946.749 kb on - strandat 5946.749 kb on - strandat 5946.756 kb on + strandat 5946.756 kb on + strandat 5946.757 kb on - strandat 5946.769 kb on - strandat 5946.875 kb on + strandat 5946.876 kb on - strandat 5946.991 kb on + strandat 5946.992 kb on - strandat 5946.996 kb on + strandat 5946.998 kb on + strandat 5946.999 kb on - strandat 5947.034 kb on + strandat 5947.098 kb on + strand, within BT4529at 5947.099 kb on - strand, within BT4529at 5947.101 kb on - strand, within BT4529at 5947.109 kb on - strand, within BT4529at 5947.113 kb on - strand, within BT4529at 5947.297 kb on - strandat 5947.315 kb on + strandat 5947.316 kb on - strandat 5947.350 kb on - strandat 5947.385 kb on + strandat 5947.386 kb on - strandat 5947.434 kb on - strandat 5947.442 kb on + strand, within BT4530at 5947.548 kb on + strand, within BT4530at 5947.549 kb on - strand, within BT4530at 5947.560 kb on - strand, within BT4530at 5947.657 kb on + strand, within BT4530at 5947.703 kb on + strand, within BT4530at 5947.707 kb on + strand, within BT4530at 5947.726 kb on - strand, within BT4530at 5947.831 kb on - strand, within BT4530at 5947.860 kb on + strand, within BT4530at 5947.860 kb on + strand, within BT4530at 5947.895 kb on + strand, within BT4530at 5947.898 kb on - strand, within BT4530at 5947.953 kb on - strand, within BT4530at 5948.059 kb on - strand, within BT4530at 5948.089 kb on - strand, within BT4530at 5948.140 kb on + strand, within BT4530at 5948.187 kb on - strand, within BT4530at 5948.190 kb on - strand, within BT4530at 5948.279 kb on + strand, within BT4530at 5948.280 kb on - strand, within BT4530at 5948.334 kb on + strand, within BT4530at 5948.334 kb on + strand, within BT4530at 5948.334 kb on + strand, within BT4530at 5948.341 kb on - strand, within BT4530at 5948.341 kb on - strand, within BT4530at 5948.341 kb on - strand, within BT4530at 5948.365 kb on + strand, within BT4530at 5948.373 kb on - strand, within BT4530at 5948.433 kb on - strand, within BT4530at 5948.728 kb on - strandat 5948.728 kb on - strandat 5948.728 kb on - strandat 5948.752 kb on - strandat 5948.757 kb on + strandat 5948.916 kb on - strandat 5948.916 kb on - strandat 5948.918 kb on - strandat 5948.921 kb on - strandat 5948.937 kb on - strandat 5948.937 kb on - strandat 5948.937 kb on - strandat 5948.963 kb on + strandat 5948.990 kb on - strand, within BT4531at 5949.047 kb on + strand, within BT4531at 5949.048 kb on - strand, within BT4531at 5949.048 kb on - strand, within BT4531at 5949.048 kb on - strand, within BT4531at 5949.076 kb on - strand, within BT4531at 5949.150 kb on - strand, within BT4531at 5949.158 kb on - strand, within BT4531at 5949.158 kb on - strand, within BT4531at 5949.160 kb on + strand, within BT4531at 5949.211 kb on + strand, within BT4531at 5949.239 kb on - strand, within BT4531at 5949.311 kb on - strand, within BT4531at 5949.347 kb on - strand, within BT4531at 5949.461 kb on - strand, within BT4531at 5949.481 kb on - strand, within BT4531at 5949.481 kb on - strand, within BT4531at 5949.513 kb on - strand, within BT4531at 5949.527 kb on - strand, within BT4531at 5949.706 kb on - strand, within BT4531

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM
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5,946,302 - BT4528 0.53 -1.9
5,946,304 - BT4528 0.53 -1.9
5,946,327 + BT4528 0.57 -1.7
5,946,436 - BT4528 0.76 -2.4
5,946,454 + BT4528 0.79 -2.9
5,946,456 + BT4528 0.79 -1.2
5,946,483 + BT4528 0.84 -1.6
5,946,541 - +0.9
5,946,553 - -1.1
5,946,568 - -0.1
5,946,749 - -2.8
5,946,749 - +2.2
5,946,756 + -2.3
5,946,756 + -2.5
5,946,757 - +0.5
5,946,769 - -1.1
5,946,875 + +2.5
5,946,876 - -1.4
5,946,991 + -0.1
5,946,992 - -1.6
5,946,996 + +0.9
5,946,998 + +2.7
5,946,999 - -2.3
5,947,034 + +2.5
5,947,098 + BT4529 0.30 -1.1
5,947,099 - BT4529 0.30 +1.0
5,947,101 - BT4529 0.31 +1.8
5,947,109 - BT4529 0.34 +4.9
5,947,113 - BT4529 0.35 +1.0
5,947,297 - +0.9
5,947,315 + -0.2
5,947,316 - +2.0
5,947,350 - +3.8
5,947,385 + -1.9
5,947,386 - +2.1
5,947,434 - -0.1
5,947,442 + BT4530 0.10 -1.7
5,947,548 + BT4530 0.18 -0.1
5,947,549 - BT4530 0.18 +4.3
5,947,560 - BT4530 0.19 +3.7
5,947,657 + BT4530 0.26 -1.1
5,947,703 + BT4530 0.29 -3.7
5,947,707 + BT4530 0.29 -2.0
5,947,726 - BT4530 0.30 +1.7
5,947,831 - BT4530 0.38 +4.1
5,947,860 + BT4530 0.40 -2.4
5,947,860 + BT4530 0.40 -2.1
5,947,895 + BT4530 0.42 -0.8
5,947,898 - BT4530 0.43 +3.4
5,947,953 - BT4530 0.46 +3.6
5,948,059 - BT4530 0.54 +1.8
5,948,089 - BT4530 0.56 +2.9
5,948,140 + BT4530 0.60 +0.9
5,948,187 - BT4530 0.63 +4.3
5,948,190 - BT4530 0.63 +3.2
5,948,279 + BT4530 0.69 -2.6
5,948,280 - BT4530 0.69 +2.3
5,948,334 + BT4530 0.73 -0.1
5,948,334 + BT4530 0.73 -0.1
5,948,334 + BT4530 0.73 -1.0
5,948,341 - BT4530 0.74 +0.1
5,948,341 - BT4530 0.74 +1.3
5,948,341 - BT4530 0.74 +1.3
5,948,365 + BT4530 0.75 -3.1
5,948,373 - BT4530 0.76 +1.6
5,948,433 - BT4530 0.80 +2.2
5,948,728 - +0.6
5,948,728 - +4.6
5,948,728 - +3.2
5,948,752 - +2.2
5,948,757 + -1.1
5,948,916 - +2.7
5,948,916 - +3.7
5,948,918 - +1.7
5,948,921 - +3.6
5,948,937 - +2.2
5,948,937 - +2.5
5,948,937 - +1.8
5,948,963 + +3.5
5,948,990 - BT4531 0.12 +5.1
5,949,047 + BT4531 0.18 +2.6
5,949,048 - BT4531 0.18 +1.3
5,949,048 - BT4531 0.18 +3.6
5,949,048 - BT4531 0.18 +1.7
5,949,076 - BT4531 0.20 +1.1
5,949,150 - BT4531 0.27 +1.4
5,949,158 - BT4531 0.28 +1.4
5,949,158 - BT4531 0.28 +4.2
5,949,160 + BT4531 0.28 -0.1
5,949,211 + BT4531 0.33 -0.7
5,949,239 - BT4531 0.36 +3.6
5,949,311 - BT4531 0.43 +0.9
5,949,347 - BT4531 0.46 +2.1
5,949,461 - BT4531 0.57 +2.8
5,949,481 - BT4531 0.59 +4.4
5,949,481 - BT4531 0.59 +1.7
5,949,513 - BT4531 0.62 +2.6
5,949,527 - BT4531 0.63 +1.6
5,949,706 - BT4531 0.80 +0.8

Or see this region's nucleotide sequence