Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4504 and BT4505 overlap by 6 nucleotides BT4505 and BT4506 are separated by 21 nucleotides BT4506 and BT4507 are separated by 33 nucleotides
BT4504: BT4504 - conserved hypothetical protein (NCBI ptt file), at 5,919,439 to 5,920,113
BT4504
BT4505: BT4505 - hypothetical protein (NCBI ptt file), at 5,920,108 to 5,920,650
BT4505
BT4506: BT4506 - putative acetyltransferase (NCBI ptt file), at 5,920,672 to 5,922,048
BT4506
BT4507: BT4507 - beta-lactamase precursor (NCBI ptt file), at 5,922,082 to 5,922,963
BT4507
Position (kb)
5920
5921
5922
5923 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5919.801 kb on - strand, within BT4504 at 5919.801 kb on - strand, within BT4504 at 5919.801 kb on - strand, within BT4504 at 5919.859 kb on - strand, within BT4504 at 5919.888 kb on + strand, within BT4504 at 5919.936 kb on + strand, within BT4504 at 5919.936 kb on + strand, within BT4504 at 5919.936 kb on + strand, within BT4504 at 5919.937 kb on - strand, within BT4504 at 5919.937 kb on - strand, within BT4504 at 5919.953 kb on + strand, within BT4504 at 5920.061 kb on + strand at 5920.062 kb on - strand at 5920.092 kb on - strand at 5920.107 kb on - strand at 5920.109 kb on + strand at 5920.218 kb on + strand, within BT4505 at 5920.218 kb on + strand, within BT4505 at 5920.218 kb on + strand, within BT4505 at 5920.219 kb on - strand, within BT4505 at 5920.219 kb on - strand, within BT4505 at 5920.219 kb on - strand, within BT4505 at 5920.366 kb on + strand, within BT4505 at 5920.366 kb on + strand, within BT4505 at 5920.381 kb on + strand, within BT4505 at 5920.382 kb on - strand, within BT4505 at 5920.426 kb on - strand, within BT4505 at 5920.426 kb on - strand, within BT4505 at 5920.438 kb on + strand, within BT4505 at 5920.494 kb on + strand, within BT4505 at 5920.494 kb on + strand, within BT4505 at 5920.587 kb on - strand, within BT4505 at 5920.642 kb on + strand at 5920.643 kb on - strand at 5920.668 kb on + strand at 5920.696 kb on + strand at 5920.842 kb on - strand, within BT4506 at 5920.842 kb on - strand, within BT4506 at 5920.846 kb on + strand, within BT4506 at 5920.847 kb on - strand, within BT4506 at 5920.888 kb on + strand, within BT4506 at 5920.985 kb on + strand, within BT4506 at 5921.007 kb on + strand, within BT4506 at 5921.009 kb on + strand, within BT4506 at 5921.087 kb on + strand, within BT4506 at 5921.087 kb on + strand, within BT4506 at 5921.110 kb on + strand, within BT4506 at 5921.123 kb on + strand, within BT4506 at 5921.141 kb on + strand, within BT4506 at 5921.141 kb on + strand, within BT4506 at 5921.142 kb on - strand, within BT4506 at 5921.146 kb on - strand, within BT4506 at 5921.189 kb on + strand, within BT4506 at 5921.318 kb on + strand, within BT4506 at 5921.352 kb on - strand, within BT4506 at 5921.361 kb on + strand, within BT4506 at 5921.361 kb on + strand, within BT4506 at 5921.361 kb on + strand, within BT4506 at 5921.440 kb on - strand, within BT4506 at 5921.440 kb on - strand at 5921.464 kb on + strand, within BT4506 at 5921.467 kb on - strand, within BT4506 at 5921.721 kb on - strand, within BT4506 at 5921.749 kb on + strand, within BT4506 at 5921.753 kb on - strand, within BT4506 at 5921.757 kb on + strand, within BT4506 at 5921.759 kb on + strand, within BT4506 at 5921.924 kb on + strand at 5921.925 kb on - strand at 5921.952 kb on + strand at 5921.953 kb on - strand at 5921.954 kb on + strand at 5921.990 kb on + strand at 5922.040 kb on + strand at 5922.061 kb on + strand at 5922.061 kb on + strand at 5922.071 kb on - strand at 5922.135 kb on + strand at 5922.181 kb on + strand, within BT4507 at 5922.181 kb on + strand, within BT4507 at 5922.182 kb on - strand, within BT4507 at 5922.183 kb on + strand, within BT4507 at 5922.184 kb on - strand, within BT4507 at 5922.184 kb on - strand, within BT4507 at 5922.189 kb on - strand, within BT4507 at 5922.189 kb on - strand, within BT4507 at 5922.371 kb on + strand, within BT4507 at 5922.372 kb on - strand, within BT4507 at 5922.627 kb on - strand, within BT4507 at 5922.743 kb on + strand, within BT4507 at 5922.779 kb on - strand, within BT4507 at 5922.818 kb on - strand, within BT4507 at 5922.921 kb on + strand at 5922.922 kb on - strand at 5922.990 kb on - strand at 5923.017 kb on + strand at 5923.018 kb on - strand at 5923.030 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 5,919,801 - BT4504 0.54 -1.7 5,919,801 - BT4504 0.54 +0.9 5,919,801 - BT4504 0.54 +1.2 5,919,859 - BT4504 0.62 -1.1 5,919,888 + BT4504 0.67 -1.0 5,919,936 + BT4504 0.74 -1.5 5,919,936 + BT4504 0.74 +0.0 5,919,936 + BT4504 0.74 +1.5 5,919,937 - BT4504 0.74 -3.4 5,919,937 - BT4504 0.74 -2.2 5,919,953 + BT4504 0.76 +1.1 5,920,061 + -4.0 5,920,062 - -3.7 5,920,092 - -1.1 5,920,107 - +1.9 5,920,109 + +0.7 5,920,218 + BT4505 0.20 +0.7 5,920,218 + BT4505 0.20 -4.2 5,920,218 + BT4505 0.20 +0.4 5,920,219 - BT4505 0.20 +0.5 5,920,219 - BT4505 0.20 +2.7 5,920,219 - BT4505 0.20 -0.1 5,920,366 + BT4505 0.48 -2.4 5,920,366 + BT4505 0.48 -0.1 5,920,381 + BT4505 0.50 -3.3 5,920,382 - BT4505 0.50 -2.4 5,920,426 - BT4505 0.59 -2.5 5,920,426 - BT4505 0.59 -3.8 5,920,438 + BT4505 0.61 +0.7 5,920,494 + BT4505 0.71 +0.5 5,920,494 + BT4505 0.71 -2.3 5,920,587 - BT4505 0.88 +2.7 5,920,642 + -2.1 5,920,643 - +1.4 5,920,668 + +1.5 5,920,696 + -4.3 5,920,842 - BT4506 0.12 -2.0 5,920,842 - BT4506 0.12 -1.5 5,920,846 + BT4506 0.13 +1.5 5,920,847 - BT4506 0.13 +1.9 5,920,888 + BT4506 0.16 -0.0 5,920,985 + BT4506 0.23 -0.5 5,921,007 + BT4506 0.24 -2.2 5,921,009 + BT4506 0.24 -1.3 5,921,087 + BT4506 0.30 +0.9 5,921,087 + BT4506 0.30 -2.2 5,921,110 + BT4506 0.32 -2.9 5,921,123 + BT4506 0.33 -0.4 5,921,141 + BT4506 0.34 +3.2 5,921,141 + BT4506 0.34 -1.0 5,921,142 - BT4506 0.34 -2.8 5,921,146 - BT4506 0.34 -1.4 5,921,189 + BT4506 0.38 +2.0 5,921,318 + BT4506 0.47 -0.7 5,921,352 - BT4506 0.49 -3.1 5,921,361 + BT4506 0.50 +1.9 5,921,361 + BT4506 0.50 +0.3 5,921,361 + BT4506 0.50 -1.4 5,921,440 - BT4506 0.56 -2.4 5,921,440 - -0.1 5,921,464 + BT4506 0.58 -2.4 5,921,467 - BT4506 0.58 -0.7 5,921,721 - BT4506 0.76 +2.7 5,921,749 + BT4506 0.78 -3.6 5,921,753 - BT4506 0.79 +1.7 5,921,757 + BT4506 0.79 -2.2 5,921,759 + BT4506 0.79 -0.6 5,921,924 + -0.3 5,921,925 - -2.1 5,921,952 + -2.1 5,921,953 - +2.5 5,921,954 + +2.0 5,921,990 + +2.9 5,922,040 + -1.8 5,922,061 + -0.1 5,922,061 + -2.2 5,922,071 - +1.2 5,922,135 + -0.1 5,922,181 + BT4507 0.11 +1.2 5,922,181 + BT4507 0.11 +1.9 5,922,182 - BT4507 0.11 +1.5 5,922,183 + BT4507 0.11 -2.9 5,922,184 - BT4507 0.12 -1.9 5,922,184 - BT4507 0.12 +2.5 5,922,189 - BT4507 0.12 +0.9 5,922,189 - BT4507 0.12 -0.1 5,922,371 + BT4507 0.33 -1.5 5,922,372 - BT4507 0.33 -1.6 5,922,627 - BT4507 0.62 -2.4 5,922,743 + BT4507 0.75 -0.1 5,922,779 - BT4507 0.79 -1.7 5,922,818 - BT4507 0.83 -0.3 5,922,921 + +0.6 5,922,922 - -0.1 5,922,990 - +0.3 5,923,017 + +1.9 5,923,018 - -0.1 5,923,030 + -1.7
Or see this region's nucleotide sequence