Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4471 and BT4472 are separated by 72 nucleotides BT4472 and BT4473 are separated by 58 nucleotides BT4473 and BT4474 are separated by 76 nucleotides
BT4471: BT4471 - hypothetical protein (NCBI ptt file), at 5,882,179 to 5,883,774
BT4471
BT4472: BT4472 - hypothetical protein (NCBI ptt file), at 5,883,847 to 5,884,554
BT4472
BT4473: BT4473 - putative purine permease (NCBI ptt file), at 5,884,613 to 5,885,923
BT4473
BT4474: BT4474 - conserved hypothetical protein (NCBI ptt file), at 5,886,000 to 5,888,162
BT4474
Position (kb)
5884
5885
5886 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 5883.619 kb on - strand at 5883.688 kb on + strand at 5883.688 kb on + strand at 5883.733 kb on - strand at 5883.800 kb on - strand at 5883.816 kb on - strand at 5883.880 kb on - strand at 5883.953 kb on - strand, within BT4472 at 5884.027 kb on - strand, within BT4472 at 5884.051 kb on + strand, within BT4472 at 5884.201 kb on - strand, within BT4472 at 5884.205 kb on - strand, within BT4472 at 5884.207 kb on - strand, within BT4472 at 5884.260 kb on + strand, within BT4472 at 5884.306 kb on + strand, within BT4472 at 5884.307 kb on - strand, within BT4472 at 5884.307 kb on - strand, within BT4472 at 5884.307 kb on - strand, within BT4472 at 5884.444 kb on - strand, within BT4472 at 5884.488 kb on + strand at 5884.489 kb on - strand at 5884.513 kb on + strand at 5884.513 kb on + strand at 5884.514 kb on - strand at 5884.517 kb on + strand at 5884.585 kb on + strand at 5884.586 kb on - strand at 5884.657 kb on + strand at 5884.705 kb on - strand at 5884.714 kb on + strand at 5884.715 kb on - strand at 5884.727 kb on - strand at 5884.780 kb on + strand, within BT4473 at 5884.781 kb on - strand, within BT4473 at 5884.781 kb on - strand, within BT4473 at 5884.803 kb on + strand, within BT4473 at 5884.803 kb on + strand, within BT4473 at 5884.814 kb on - strand, within BT4473 at 5884.815 kb on + strand, within BT4473 at 5884.816 kb on - strand, within BT4473 at 5884.892 kb on - strand, within BT4473 at 5884.897 kb on - strand, within BT4473 at 5884.942 kb on + strand, within BT4473 at 5884.943 kb on - strand, within BT4473 at 5885.000 kb on - strand, within BT4473 at 5885.087 kb on + strand, within BT4473 at 5885.089 kb on + strand, within BT4473 at 5885.090 kb on - strand, within BT4473 at 5885.149 kb on - strand, within BT4473 at 5885.264 kb on - strand, within BT4473 at 5885.351 kb on - strand, within BT4473 at 5885.509 kb on + strand, within BT4473 at 5885.511 kb on + strand, within BT4473 at 5885.589 kb on + strand, within BT4473 at 5885.589 kb on + strand, within BT4473 at 5885.589 kb on + strand, within BT4473 at 5885.590 kb on - strand, within BT4473 at 5885.597 kb on + strand, within BT4473 at 5885.598 kb on - strand, within BT4473 at 5885.609 kb on - strand, within BT4473 at 5885.640 kb on + strand, within BT4473 at 5885.647 kb on + strand, within BT4473 at 5885.647 kb on + strand, within BT4473 at 5885.766 kb on + strand, within BT4473 at 5885.900 kb on - strand at 5885.911 kb on + strand at 5885.912 kb on - strand at 5885.942 kb on + strand at 5885.943 kb on - strand at 5885.943 kb on - strand at 5885.945 kb on + strand at 5885.945 kb on + strand at 5885.946 kb on - strand at 5885.987 kb on + strand at 5885.990 kb on + strand at 5885.997 kb on + strand at 5886.035 kb on + strand at 5886.104 kb on + strand at 5886.104 kb on + strand at 5886.125 kb on - strand at 5886.125 kb on - strand at 5886.162 kb on + strand at 5886.163 kb on - strand at 5886.196 kb on + strand at 5886.196 kb on + strand at 5886.221 kb on + strand, within BT4474 at 5886.221 kb on + strand, within BT4474 at 5886.221 kb on + strand, within BT4474 at 5886.221 kb on + strand, within BT4474 at 5886.227 kb on + strand, within BT4474 at 5886.288 kb on + strand, within BT4474 at 5886.465 kb on - strand, within BT4474 at 5886.590 kb on + strand, within BT4474 at 5886.592 kb on + strand, within BT4474 at 5886.595 kb on + strand, within BT4474 at 5886.639 kb on - strand, within BT4474 at 5886.639 kb on - strand, within BT4474 at 5886.639 kb on - strand, within BT4474 at 5886.641 kb on + strand, within BT4474 at 5886.642 kb on - strand, within BT4474 at 5886.642 kb on - strand, within BT4474 at 5886.668 kb on + strand, within BT4474 at 5886.669 kb on - strand, within BT4474 at 5886.669 kb on - strand, within BT4474 at 5886.729 kb on - strand, within BT4474 at 5886.729 kb on - strand, within BT4474 at 5886.863 kb on - strand, within BT4474
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 5,883,619 - -0.1 5,883,688 + -0.6 5,883,688 + +0.6 5,883,733 - -1.4 5,883,800 - -4.9 5,883,816 - +1.9 5,883,880 - -4.0 5,883,953 - BT4472 0.15 -2.6 5,884,027 - BT4472 0.25 -1.6 5,884,051 + BT4472 0.29 -0.1 5,884,201 - BT4472 0.50 -0.5 5,884,205 - BT4472 0.51 -2.6 5,884,207 - BT4472 0.51 -0.1 5,884,260 + BT4472 0.58 +2.9 5,884,306 + BT4472 0.65 -1.6 5,884,307 - BT4472 0.65 -2.2 5,884,307 - BT4472 0.65 -2.1 5,884,307 - BT4472 0.65 -1.1 5,884,444 - BT4472 0.84 -0.1 5,884,488 + -1.1 5,884,489 - -0.1 5,884,513 + -1.6 5,884,513 + +2.9 5,884,514 - -3.2 5,884,517 + -0.4 5,884,585 + +0.4 5,884,586 - -2.9 5,884,657 + -4.4 5,884,705 - -2.2 5,884,714 + -2.1 5,884,715 - +1.5 5,884,727 - -0.8 5,884,780 + BT4473 0.13 -0.1 5,884,781 - BT4473 0.13 -1.1 5,884,781 - BT4473 0.13 -1.1 5,884,803 + BT4473 0.14 -2.1 5,884,803 + BT4473 0.14 -1.7 5,884,814 - BT4473 0.15 -1.5 5,884,815 + BT4473 0.15 +0.9 5,884,816 - BT4473 0.15 -2.6 5,884,892 - BT4473 0.21 -3.2 5,884,897 - BT4473 0.22 -2.1 5,884,942 + BT4473 0.25 +0.9 5,884,943 - BT4473 0.25 -1.9 5,885,000 - BT4473 0.30 -3.2 5,885,087 + BT4473 0.36 -2.6 5,885,089 + BT4473 0.36 -1.8 5,885,090 - BT4473 0.36 -2.4 5,885,149 - BT4473 0.41 -0.7 5,885,264 - BT4473 0.50 -1.1 5,885,351 - BT4473 0.56 -0.9 5,885,509 + BT4473 0.68 -1.8 5,885,511 + BT4473 0.68 -3.2 5,885,589 + BT4473 0.74 +0.1 5,885,589 + BT4473 0.74 -3.4 5,885,589 + BT4473 0.74 +2.5 5,885,590 - BT4473 0.75 -3.5 5,885,597 + BT4473 0.75 -0.1 5,885,598 - BT4473 0.75 -1.2 5,885,609 - BT4473 0.76 -3.5 5,885,640 + BT4473 0.78 -1.1 5,885,647 + BT4473 0.79 -2.4 5,885,647 + BT4473 0.79 -0.5 5,885,766 + BT4473 0.88 -1.1 5,885,900 - -3.1 5,885,911 + -1.6 5,885,912 - -0.1 5,885,942 + +1.5 5,885,943 - +2.6 5,885,943 - -0.4 5,885,945 + -0.3 5,885,945 + +2.5 5,885,946 - +0.5 5,885,987 + -2.6 5,885,990 + -1.2 5,885,997 + +1.1 5,886,035 + +2.7 5,886,104 + +2.3 5,886,104 + -2.4 5,886,125 - -1.1 5,886,125 - -2.1 5,886,162 + +4.1 5,886,163 - +1.7 5,886,196 + -4.1 5,886,196 + -1.3 5,886,221 + BT4474 0.10 -3.3 5,886,221 + BT4474 0.10 +1.6 5,886,221 + BT4474 0.10 -2.9 5,886,221 + BT4474 0.10 +3.3 5,886,227 + BT4474 0.10 -0.3 5,886,288 + BT4474 0.13 -0.5 5,886,465 - BT4474 0.21 +1.7 5,886,590 + BT4474 0.27 -3.3 5,886,592 + BT4474 0.27 -1.6 5,886,595 + BT4474 0.28 -2.7 5,886,639 - BT4474 0.30 -2.1 5,886,639 - BT4474 0.30 -2.4 5,886,639 - BT4474 0.30 -3.2 5,886,641 + BT4474 0.30 -2.2 5,886,642 - BT4474 0.30 -2.9 5,886,642 - BT4474 0.30 -0.1 5,886,668 + BT4474 0.31 -0.9 5,886,669 - BT4474 0.31 +2.4 5,886,669 - BT4474 0.31 -3.4 5,886,729 - BT4474 0.34 -1.4 5,886,729 - BT4474 0.34 +1.4 5,886,863 - BT4474 0.40 -0.5
Or see this region's nucleotide sequence