Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4431 and BT4432 are separated by 4 nucleotides BT4432 and BT4433 are separated by 694 nucleotides
BT4431: BT4431 - hypothetical protein (NCBI ptt file), at 5,838,055 to 5,839,854
BT4431
BT4432: BT4432 - putative non-specific DNA-binding protein HU-1 (NCBI ptt file), at 5,839,859 to 5,840,272
BT4432
BT4433: BT4433 - hypothetical protein (NCBI ptt file), at 5,840,967 to 5,841,416
BT4433
Position (kb) 5839
5840
5841 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5838.971 kb on + strand, within BT4431 at 5838.972 kb on - strand, within BT4431 at 5839.015 kb on + strand, within BT4431 at 5839.015 kb on + strand, within BT4431 at 5839.016 kb on - strand, within BT4431 at 5839.016 kb on - strand, within BT4431 at 5839.116 kb on - strand, within BT4431 at 5839.116 kb on - strand, within BT4431 at 5839.154 kb on - strand, within BT4431 at 5839.195 kb on - strand, within BT4431 at 5839.196 kb on - strand, within BT4431 at 5839.209 kb on + strand, within BT4431 at 5839.209 kb on + strand, within BT4431 at 5839.269 kb on + strand, within BT4431 at 5839.493 kb on + strand, within BT4431 at 5839.493 kb on + strand, within BT4431 at 5839.494 kb on - strand, within BT4431 at 5839.497 kb on + strand, within BT4431 at 5839.498 kb on - strand, within BT4431 at 5839.498 kb on - strand, within BT4431 at 5839.498 kb on - strand, within BT4431 at 5839.504 kb on - strand, within BT4431 at 5839.529 kb on - strand, within BT4431 at 5839.624 kb on - strand, within BT4431 at 5839.676 kb on - strand at 5839.693 kb on - strand at 5840.133 kb on - strand, within BT4432 at 5840.160 kb on - strand, within BT4432 at 5840.160 kb on - strand, within BT4432 at 5840.222 kb on - strand, within BT4432 at 5840.222 kb on - strand, within BT4432 at 5840.222 kb on - strand, within BT4432 at 5840.265 kb on - strand at 5840.276 kb on - strand at 5840.346 kb on - strand at 5840.426 kb on - strand at 5840.454 kb on - strand at 5840.509 kb on - strand at 5840.650 kb on - strand at 5840.680 kb on - strand at 5840.680 kb on - strand at 5840.683 kb on - strand at 5840.719 kb on - strand at 5840.871 kb on - strand at 5840.946 kb on + strand at 5840.953 kb on - strand at 5840.967 kb on - strand at 5841.062 kb on - strand, within BT4433 at 5841.080 kb on + strand, within BT4433 at 5841.083 kb on - strand, within BT4433 at 5841.083 kb on - strand, within BT4433 at 5841.139 kb on + strand, within BT4433 at 5841.176 kb on + strand, within BT4433 at 5841.177 kb on - strand, within BT4433 at 5841.224 kb on - strand, within BT4433
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 5,838,971 + BT4431 0.51 -4.7 5,838,972 - BT4431 0.51 -2.0 5,839,015 + BT4431 0.53 -1.6 5,839,015 + BT4431 0.53 +1.0 5,839,016 - BT4431 0.53 -0.0 5,839,016 - BT4431 0.53 +0.8 5,839,116 - BT4431 0.59 +1.3 5,839,116 - BT4431 0.59 -1.8 5,839,154 - BT4431 0.61 -1.8 5,839,195 - BT4431 0.63 -0.5 5,839,196 - BT4431 0.63 -0.3 5,839,209 + BT4431 0.64 +2.0 5,839,209 + BT4431 0.64 +0.7 5,839,269 + BT4431 0.67 +0.4 5,839,493 + BT4431 0.80 +1.0 5,839,493 + BT4431 0.80 -1.8 5,839,494 - BT4431 0.80 +2.0 5,839,497 + BT4431 0.80 -2.6 5,839,498 - BT4431 0.80 +2.6 5,839,498 - BT4431 0.80 -0.8 5,839,498 - BT4431 0.80 +2.0 5,839,504 - BT4431 0.81 -2.0 5,839,529 - BT4431 0.82 -0.4 5,839,624 - BT4431 0.87 +1.0 5,839,676 - -3.3 5,839,693 - -2.5 5,840,133 - BT4432 0.66 -2.3 5,840,160 - BT4432 0.73 -3.6 5,840,160 - BT4432 0.73 -2.5 5,840,222 - BT4432 0.88 -2.8 5,840,222 - BT4432 0.88 +1.0 5,840,222 - BT4432 0.88 -0.4 5,840,265 - -1.6 5,840,276 - -0.6 5,840,346 - -1.3 5,840,426 - -4.0 5,840,454 - -0.6 5,840,509 - -0.0 5,840,650 - -2.0 5,840,680 - -1.3 5,840,680 - +0.2 5,840,683 - +1.6 5,840,719 - +1.2 5,840,871 - -1.6 5,840,946 + -0.6 5,840,953 - -2.7 5,840,967 - -0.0 5,841,062 - BT4433 0.21 -3.8 5,841,080 + BT4433 0.25 -2.8 5,841,083 - BT4433 0.26 +0.9 5,841,083 - BT4433 0.26 -0.8 5,841,139 + BT4433 0.38 -3.7 5,841,176 + BT4433 0.46 -3.3 5,841,177 - BT4433 0.47 -1.8 5,841,224 - BT4433 0.57 -0.8
Or see this region's nucleotide sequence