Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4342 and BT4343 are separated by 36 nucleotides BT4343 and BT4344 are separated by 65 nucleotides
BT4342: BT4342 - RNA-directed DNA polymerase (NCBI ptt file), at 5,720,067 to 5,721,200
BT4342
BT4343: BT4343 - conserved hypothetical protein (NCBI ptt file), at 5,721,237 to 5,722,175
BT4343
BT4344: BT4344 - conserved hypothetical protein, putative DNA helicase (NCBI ptt file), at 5,722,241 to 5,727,058
BT4344
Position (kb) 5721
5722
5723 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5720.245 kb on + strand, within BT4342 at 5720.460 kb on - strand, within BT4342 at 5720.463 kb on + strand, within BT4342 at 5720.464 kb on - strand, within BT4342 at 5720.471 kb on + strand, within BT4342 at 5720.473 kb on + strand, within BT4342 at 5720.473 kb on + strand, within BT4342 at 5720.474 kb on - strand, within BT4342 at 5720.474 kb on - strand, within BT4342 at 5720.474 kb on - strand, within BT4342 at 5720.476 kb on + strand, within BT4342 at 5720.485 kb on + strand, within BT4342 at 5720.565 kb on + strand, within BT4342 at 5720.658 kb on - strand, within BT4342 at 5720.687 kb on + strand, within BT4342 at 5720.799 kb on + strand, within BT4342 at 5720.809 kb on - strand, within BT4342 at 5720.822 kb on + strand, within BT4342 at 5720.934 kb on + strand, within BT4342 at 5720.935 kb on - strand, within BT4342 at 5720.947 kb on + strand, within BT4342 at 5721.107 kb on - strand at 5721.134 kb on - strand at 5721.150 kb on + strand at 5721.278 kb on - strand at 5721.303 kb on - strand at 5721.411 kb on + strand, within BT4343 at 5721.488 kb on + strand, within BT4343 at 5721.490 kb on + strand, within BT4343 at 5721.558 kb on + strand, within BT4343 at 5721.558 kb on + strand, within BT4343 at 5721.559 kb on - strand, within BT4343 at 5721.591 kb on + strand, within BT4343 at 5721.592 kb on - strand, within BT4343 at 5721.592 kb on - strand, within BT4343 at 5721.603 kb on + strand, within BT4343 at 5721.654 kb on - strand, within BT4343 at 5721.749 kb on - strand, within BT4343 at 5721.761 kb on + strand, within BT4343 at 5721.761 kb on + strand, within BT4343 at 5721.805 kb on + strand, within BT4343 at 5721.805 kb on + strand, within BT4343 at 5721.806 kb on - strand, within BT4343 at 5721.903 kb on + strand, within BT4343 at 5721.969 kb on - strand, within BT4343 at 5721.977 kb on + strand, within BT4343 at 5722.048 kb on + strand, within BT4343 at 5722.074 kb on + strand, within BT4343 at 5722.114 kb on - strand at 5722.140 kb on + strand at 5722.149 kb on - strand at 5722.152 kb on + strand at 5722.157 kb on + strand at 5722.157 kb on + strand at 5722.157 kb on + strand at 5722.187 kb on + strand at 5722.201 kb on + strand at 5722.304 kb on - strand at 5722.464 kb on + strand at 5722.465 kb on - strand at 5722.482 kb on - strand at 5722.482 kb on - strand at 5722.482 kb on - strand at 5722.744 kb on - strand, within BT4344 at 5722.813 kb on + strand, within BT4344 at 5722.814 kb on - strand, within BT4344 at 5722.821 kb on + strand, within BT4344 at 5722.824 kb on + strand, within BT4344 at 5722.884 kb on + strand, within BT4344 at 5722.897 kb on + strand, within BT4344 at 5722.924 kb on + strand, within BT4344 at 5723.021 kb on + strand, within BT4344 at 5723.122 kb on + strand, within BT4344 at 5723.124 kb on + strand, within BT4344 at 5723.125 kb on - strand, within BT4344 at 5723.139 kb on - strand, within BT4344
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 5,720,245 + BT4342 0.16 -1.0 5,720,460 - BT4342 0.35 -0.0 5,720,463 + BT4342 0.35 -0.0 5,720,464 - BT4342 0.35 -1.5 5,720,471 + BT4342 0.36 -1.8 5,720,473 + BT4342 0.36 -1.0 5,720,473 + BT4342 0.36 -0.0 5,720,474 - BT4342 0.36 +1.6 5,720,474 - BT4342 0.36 -1.8 5,720,474 - BT4342 0.36 -0.0 5,720,476 + BT4342 0.36 -2.6 5,720,485 + BT4342 0.37 -2.0 5,720,565 + BT4342 0.44 -0.0 5,720,658 - BT4342 0.52 -1.3 5,720,687 + BT4342 0.55 -0.0 5,720,799 + BT4342 0.65 +0.5 5,720,809 - BT4342 0.65 +1.8 5,720,822 + BT4342 0.67 -0.0 5,720,934 + BT4342 0.76 -1.0 5,720,935 - BT4342 0.77 +3.6 5,720,947 + BT4342 0.78 -0.0 5,721,107 - +3.3 5,721,134 - -0.0 5,721,150 + +1.9 5,721,278 - -1.0 5,721,303 - -3.2 5,721,411 + BT4343 0.19 +2.7 5,721,488 + BT4343 0.27 -1.6 5,721,490 + BT4343 0.27 -2.0 5,721,558 + BT4343 0.34 -1.0 5,721,558 + BT4343 0.34 +2.8 5,721,559 - BT4343 0.34 +2.2 5,721,591 + BT4343 0.38 +2.0 5,721,592 - BT4343 0.38 +1.3 5,721,592 - BT4343 0.38 -2.3 5,721,603 + BT4343 0.39 -1.0 5,721,654 - BT4343 0.44 -0.0 5,721,749 - BT4343 0.55 -2.0 5,721,761 + BT4343 0.56 -2.6 5,721,761 + BT4343 0.56 +3.4 5,721,805 + BT4343 0.60 -2.6 5,721,805 + BT4343 0.60 +2.2 5,721,806 - BT4343 0.61 -2.0 5,721,903 + BT4343 0.71 +0.6 5,721,969 - BT4343 0.78 +0.4 5,721,977 + BT4343 0.79 +0.9 5,722,048 + BT4343 0.86 +2.0 5,722,074 + BT4343 0.89 +2.6 5,722,114 - -1.0 5,722,140 + +3.3 5,722,149 - -2.3 5,722,152 + -0.0 5,722,157 + -2.3 5,722,157 + -1.5 5,722,157 + -1.6 5,722,187 + -1.2 5,722,201 + -0.9 5,722,304 - +0.3 5,722,464 + -0.5 5,722,465 - -2.1 5,722,482 - +2.0 5,722,482 - -2.3 5,722,482 - +2.1 5,722,744 - BT4344 0.10 -0.0 5,722,813 + BT4344 0.12 +3.7 5,722,814 - BT4344 0.12 -1.7 5,722,821 + BT4344 0.12 +0.8 5,722,824 + BT4344 0.12 -1.2 5,722,884 + BT4344 0.13 +1.5 5,722,897 + BT4344 0.14 -0.7 5,722,924 + BT4344 0.14 -2.2 5,723,021 + BT4344 0.16 -1.3 5,723,122 + BT4344 0.18 -1.4 5,723,124 + BT4344 0.18 -1.8 5,723,125 - BT4344 0.18 -2.0 5,723,139 - BT4344 0.19 +1.5
Or see this region's nucleotide sequence