Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4312 and BT4313 are separated by 149 nucleotides BT4313 and BT4314 are separated by 21 nucleotides BT4314 and BT4315 are separated by 159 nucleotides BT4315 and BT4316 overlap by 1 nucleotides BT4316 and BT4317 are separated by 26 nucleotides
BT4312: BT4312 - seryl-tRNA synthetase (NCBI ptt file), at 5,682,813 to 5,684,087
BT4312
BT4313: BT4313 - 50S ribosomal protein L27 (NCBI ptt file), at 5,684,237 to 5,684,506
BT4313
BT4314: BT4314 - 50S ribosomal protein L21 (NCBI ptt file), at 5,684,528 to 5,684,845
BT4314
BT4315: BT4315 - HPr(Ser) phosphatase (NCBI ptt file), at 5,685,005 to 5,685,646
BT4315
BT4316: BT4316 - conserved hypothetical protein (NCBI ptt file), at 5,685,646 to 5,686,530
BT4316
BT4317: BT4317 - putative lipoprotein protein (NCBI ptt file), at 5,686,557 to 5,687,069
BT4317
Position (kb) 5685
5686 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5684.201 kb on - strand at 5684.232 kb on - strand at 5685.010 kb on + strand at 5685.012 kb on + strand at 5685.012 kb on + strand at 5685.037 kb on + strand at 5685.107 kb on + strand, within BT4315 at 5685.107 kb on + strand, within BT4315 at 5685.142 kb on + strand, within BT4315 at 5685.157 kb on - strand, within BT4315 at 5685.158 kb on + strand, within BT4315 at 5685.233 kb on + strand, within BT4315 at 5685.233 kb on + strand, within BT4315 at 5685.233 kb on + strand, within BT4315 at 5685.233 kb on + strand, within BT4315 at 5685.233 kb on + strand, within BT4315 at 5685.234 kb on - strand, within BT4315 at 5685.254 kb on + strand, within BT4315 at 5685.255 kb on - strand, within BT4315 at 5685.255 kb on - strand, within BT4315 at 5685.279 kb on + strand, within BT4315 at 5685.280 kb on - strand, within BT4315 at 5685.295 kb on + strand, within BT4315 at 5685.354 kb on - strand, within BT4315 at 5685.429 kb on + strand, within BT4315 at 5685.430 kb on - strand, within BT4315 at 5685.430 kb on - strand, within BT4315 at 5685.505 kb on + strand, within BT4315 at 5685.514 kb on + strand, within BT4315 at 5685.515 kb on - strand, within BT4315 at 5685.544 kb on + strand, within BT4315 at 5685.544 kb on + strand, within BT4315 at 5685.545 kb on - strand, within BT4315 at 5685.581 kb on - strand, within BT4315 at 5685.610 kb on + strand at 5685.662 kb on - strand at 5685.666 kb on + strand at 5685.666 kb on + strand at 5685.666 kb on + strand at 5685.667 kb on - strand at 5685.787 kb on - strand, within BT4316 at 5685.993 kb on - strand at 5685.995 kb on - strand, within BT4316 at 5686.070 kb on + strand, within BT4316 at 5686.070 kb on + strand, within BT4316 at 5686.075 kb on - strand, within BT4316 at 5686.119 kb on - strand, within BT4316 at 5686.246 kb on - strand, within BT4316 at 5686.268 kb on - strand, within BT4316 at 5686.337 kb on + strand, within BT4316 at 5686.427 kb on + strand, within BT4316 at 5686.428 kb on - strand, within BT4316 at 5686.428 kb on - strand, within BT4316 at 5686.441 kb on - strand, within BT4316 at 5686.456 kb on + strand at 5686.492 kb on + strand at 5686.492 kb on + strand at 5686.501 kb on - strand at 5686.537 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 5,684,201 - -2.3 5,684,232 - -1.5 5,685,010 + -3.3 5,685,012 + -2.0 5,685,012 + +2.0 5,685,037 + -3.3 5,685,107 + BT4315 0.16 -3.0 5,685,107 + BT4315 0.16 -1.5 5,685,142 + BT4315 0.21 -2.8 5,685,157 - BT4315 0.24 -0.0 5,685,158 + BT4315 0.24 -1.7 5,685,233 + BT4315 0.36 -2.6 5,685,233 + BT4315 0.36 -2.0 5,685,233 + BT4315 0.36 -0.6 5,685,233 + BT4315 0.36 -1.0 5,685,233 + BT4315 0.36 -3.6 5,685,234 - BT4315 0.36 -0.0 5,685,254 + BT4315 0.39 -0.0 5,685,255 - BT4315 0.39 -4.2 5,685,255 - BT4315 0.39 -2.5 5,685,279 + BT4315 0.43 -0.0 5,685,280 - BT4315 0.43 -0.6 5,685,295 + BT4315 0.45 -3.0 5,685,354 - BT4315 0.54 -1.0 5,685,429 + BT4315 0.66 -1.4 5,685,430 - BT4315 0.66 -2.1 5,685,430 - BT4315 0.66 -1.0 5,685,505 + BT4315 0.78 -1.6 5,685,514 + BT4315 0.79 +2.0 5,685,515 - BT4315 0.79 -1.0 5,685,544 + BT4315 0.84 -0.0 5,685,544 + BT4315 0.84 -3.6 5,685,545 - BT4315 0.84 +1.6 5,685,581 - BT4315 0.90 -0.0 5,685,610 + -2.8 5,685,662 - -1.3 5,685,666 + -1.6 5,685,666 + -2.0 5,685,666 + -0.5 5,685,667 - +2.3 5,685,787 - BT4316 0.16 -0.5 5,685,993 - -0.0 5,685,995 - BT4316 0.39 -2.0 5,686,070 + BT4316 0.48 -1.7 5,686,070 + BT4316 0.48 -3.3 5,686,075 - BT4316 0.48 -1.5 5,686,119 - BT4316 0.53 +0.5 5,686,246 - BT4316 0.68 +2.6 5,686,268 - BT4316 0.70 -1.0 5,686,337 + BT4316 0.78 -2.0 5,686,427 + BT4316 0.88 -1.5 5,686,428 - BT4316 0.88 -0.0 5,686,428 - BT4316 0.88 +0.7 5,686,441 - BT4316 0.90 +2.5 5,686,456 + -1.2 5,686,492 + +1.0 5,686,492 + -2.0 5,686,501 - -0.0 5,686,537 - -0.5
Or see this region's nucleotide sequence