Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4311 and BT4312 are separated by 133 nucleotides BT4312 and BT4313 are separated by 149 nucleotides BT4313 and BT4314 are separated by 21 nucleotides BT4314 and BT4315 are separated by 159 nucleotides
BT4311: BT4311 - glucose/galactose transporter (NCBI ptt file), at 5,681,519 to 5,682,679
BT4311
BT4312: BT4312 - seryl-tRNA synthetase (NCBI ptt file), at 5,682,813 to 5,684,087
BT4312
BT4313: BT4313 - 50S ribosomal protein L27 (NCBI ptt file), at 5,684,237 to 5,684,506
BT4313
BT4314: BT4314 - 50S ribosomal protein L21 (NCBI ptt file), at 5,684,528 to 5,684,845
BT4314
BT4315: BT4315 - HPr(Ser) phosphatase (NCBI ptt file), at 5,685,005 to 5,685,646
BT4315
Position (kb)
5682
5683
5684
5685 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 5681.870 kb on - strand, within BT4311 at 5681.872 kb on - strand, within BT4311 at 5681.901 kb on + strand, within BT4311 at 5681.938 kb on - strand, within BT4311 at 5681.982 kb on - strand, within BT4311 at 5681.987 kb on - strand, within BT4311 at 5681.996 kb on + strand, within BT4311 at 5682.005 kb on + strand, within BT4311 at 5682.012 kb on - strand, within BT4311 at 5682.012 kb on - strand, within BT4311 at 5682.015 kb on + strand, within BT4311 at 5682.016 kb on - strand, within BT4311 at 5682.018 kb on - strand, within BT4311 at 5682.038 kb on + strand, within BT4311 at 5682.039 kb on - strand, within BT4311 at 5682.041 kb on + strand, within BT4311 at 5682.042 kb on - strand, within BT4311 at 5682.044 kb on + strand, within BT4311 at 5682.084 kb on + strand, within BT4311 at 5682.157 kb on - strand, within BT4311 at 5682.166 kb on + strand, within BT4311 at 5682.199 kb on - strand, within BT4311 at 5682.199 kb on - strand, within BT4311 at 5682.221 kb on + strand, within BT4311 at 5682.230 kb on - strand, within BT4311 at 5682.299 kb on + strand, within BT4311 at 5682.299 kb on + strand, within BT4311 at 5682.300 kb on - strand, within BT4311 at 5682.358 kb on - strand, within BT4311 at 5682.438 kb on + strand, within BT4311 at 5682.452 kb on + strand, within BT4311 at 5682.453 kb on + strand, within BT4311 at 5682.481 kb on + strand, within BT4311 at 5682.481 kb on + strand, within BT4311 at 5682.482 kb on - strand, within BT4311 at 5682.530 kb on - strand, within BT4311 at 5682.636 kb on - strand at 5682.638 kb on + strand at 5682.650 kb on - strand at 5682.755 kb on + strand at 5682.784 kb on - strand at 5682.803 kb on - strand at 5682.815 kb on - strand at 5682.820 kb on - strand at 5682.820 kb on - strand at 5684.201 kb on - strand at 5684.232 kb on - strand at 5685.010 kb on + strand at 5685.012 kb on + strand at 5685.012 kb on + strand at 5685.037 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 5,681,870 - BT4311 0.30 +0.4 5,681,872 - BT4311 0.30 -0.0 5,681,901 + BT4311 0.33 +1.8 5,681,938 - BT4311 0.36 -1.0 5,681,982 - BT4311 0.40 -1.0 5,681,987 - BT4311 0.40 -3.4 5,681,996 + BT4311 0.41 -3.2 5,682,005 + BT4311 0.42 +0.3 5,682,012 - BT4311 0.42 -1.2 5,682,012 - BT4311 0.42 +1.0 5,682,015 + BT4311 0.43 -0.0 5,682,016 - BT4311 0.43 -0.0 5,682,018 - BT4311 0.43 +3.9 5,682,038 + BT4311 0.45 -0.0 5,682,039 - BT4311 0.45 -1.6 5,682,041 + BT4311 0.45 -0.3 5,682,042 - BT4311 0.45 -2.6 5,682,044 + BT4311 0.45 +0.2 5,682,084 + BT4311 0.49 -1.2 5,682,157 - BT4311 0.55 -0.6 5,682,166 + BT4311 0.56 -1.5 5,682,199 - BT4311 0.59 -0.4 5,682,199 - BT4311 0.59 -0.0 5,682,221 + BT4311 0.60 -1.7 5,682,230 - BT4311 0.61 +0.6 5,682,299 + BT4311 0.67 -1.5 5,682,299 + BT4311 0.67 -1.6 5,682,300 - BT4311 0.67 -0.0 5,682,358 - BT4311 0.72 -1.4 5,682,438 + BT4311 0.79 +1.9 5,682,452 + BT4311 0.80 +1.0 5,682,453 + BT4311 0.80 -0.6 5,682,481 + BT4311 0.83 +2.5 5,682,481 + BT4311 0.83 -2.6 5,682,482 - BT4311 0.83 +2.3 5,682,530 - BT4311 0.87 +2.3 5,682,636 - -0.0 5,682,638 + -2.7 5,682,650 - +2.5 5,682,755 + +1.0 5,682,784 - -0.4 5,682,803 - +3.4 5,682,815 - -0.9 5,682,820 - -0.0 5,682,820 - -1.1 5,684,201 - -2.3 5,684,232 - -1.5 5,685,010 + -3.3 5,685,012 + -2.0 5,685,012 + +2.0 5,685,037 + -3.3
Or see this region's nucleotide sequence