Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4312

Experiment: BHIS with Trimethoprim 0.00625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4311 and BT4312 are separated by 133 nucleotidesBT4312 and BT4313 are separated by 149 nucleotidesBT4313 and BT4314 are separated by 21 nucleotidesBT4314 and BT4315 are separated by 159 nucleotides BT4311: BT4311 - glucose/galactose transporter (NCBI ptt file), at 5,681,519 to 5,682,679 BT4311 BT4312: BT4312 - seryl-tRNA synthetase (NCBI ptt file), at 5,682,813 to 5,684,087 BT4312 BT4313: BT4313 - 50S ribosomal protein L27 (NCBI ptt file), at 5,684,237 to 5,684,506 BT4313 BT4314: BT4314 - 50S ribosomal protein L21 (NCBI ptt file), at 5,684,528 to 5,684,845 BT4314 BT4315: BT4315 - HPr(Ser) phosphatase (NCBI ptt file), at 5,685,005 to 5,685,646 BT4315 Position (kb) 5682 5683 5684 5685Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 5681.870 kb on - strand, within BT4311at 5681.872 kb on - strand, within BT4311at 5681.901 kb on + strand, within BT4311at 5681.938 kb on - strand, within BT4311at 5681.982 kb on - strand, within BT4311at 5681.987 kb on - strand, within BT4311at 5681.996 kb on + strand, within BT4311at 5682.005 kb on + strand, within BT4311at 5682.012 kb on - strand, within BT4311at 5682.012 kb on - strand, within BT4311at 5682.015 kb on + strand, within BT4311at 5682.016 kb on - strand, within BT4311at 5682.018 kb on - strand, within BT4311at 5682.038 kb on + strand, within BT4311at 5682.039 kb on - strand, within BT4311at 5682.041 kb on + strand, within BT4311at 5682.042 kb on - strand, within BT4311at 5682.044 kb on + strand, within BT4311at 5682.084 kb on + strand, within BT4311at 5682.157 kb on - strand, within BT4311at 5682.166 kb on + strand, within BT4311at 5682.199 kb on - strand, within BT4311at 5682.199 kb on - strand, within BT4311at 5682.221 kb on + strand, within BT4311at 5682.230 kb on - strand, within BT4311at 5682.299 kb on + strand, within BT4311at 5682.299 kb on + strand, within BT4311at 5682.300 kb on - strand, within BT4311at 5682.358 kb on - strand, within BT4311at 5682.438 kb on + strand, within BT4311at 5682.452 kb on + strand, within BT4311at 5682.453 kb on + strand, within BT4311at 5682.481 kb on + strand, within BT4311at 5682.481 kb on + strand, within BT4311at 5682.482 kb on - strand, within BT4311at 5682.530 kb on - strand, within BT4311at 5682.636 kb on - strandat 5682.638 kb on + strandat 5682.650 kb on - strandat 5682.755 kb on + strandat 5682.784 kb on - strandat 5682.803 kb on - strandat 5682.815 kb on - strandat 5682.820 kb on - strandat 5682.820 kb on - strandat 5684.201 kb on - strandat 5684.232 kb on - strandat 5685.010 kb on + strandat 5685.012 kb on + strandat 5685.012 kb on + strandat 5685.037 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM
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5,681,870 - BT4311 0.30 +0.4
5,681,872 - BT4311 0.30 -0.0
5,681,901 + BT4311 0.33 +1.8
5,681,938 - BT4311 0.36 -1.0
5,681,982 - BT4311 0.40 -1.0
5,681,987 - BT4311 0.40 -3.4
5,681,996 + BT4311 0.41 -3.2
5,682,005 + BT4311 0.42 +0.3
5,682,012 - BT4311 0.42 -1.2
5,682,012 - BT4311 0.42 +1.0
5,682,015 + BT4311 0.43 -0.0
5,682,016 - BT4311 0.43 -0.0
5,682,018 - BT4311 0.43 +3.9
5,682,038 + BT4311 0.45 -0.0
5,682,039 - BT4311 0.45 -1.6
5,682,041 + BT4311 0.45 -0.3
5,682,042 - BT4311 0.45 -2.6
5,682,044 + BT4311 0.45 +0.2
5,682,084 + BT4311 0.49 -1.2
5,682,157 - BT4311 0.55 -0.6
5,682,166 + BT4311 0.56 -1.5
5,682,199 - BT4311 0.59 -0.4
5,682,199 - BT4311 0.59 -0.0
5,682,221 + BT4311 0.60 -1.7
5,682,230 - BT4311 0.61 +0.6
5,682,299 + BT4311 0.67 -1.5
5,682,299 + BT4311 0.67 -1.6
5,682,300 - BT4311 0.67 -0.0
5,682,358 - BT4311 0.72 -1.4
5,682,438 + BT4311 0.79 +1.9
5,682,452 + BT4311 0.80 +1.0
5,682,453 + BT4311 0.80 -0.6
5,682,481 + BT4311 0.83 +2.5
5,682,481 + BT4311 0.83 -2.6
5,682,482 - BT4311 0.83 +2.3
5,682,530 - BT4311 0.87 +2.3
5,682,636 - -0.0
5,682,638 + -2.7
5,682,650 - +2.5
5,682,755 + +1.0
5,682,784 - -0.4
5,682,803 - +3.4
5,682,815 - -0.9
5,682,820 - -0.0
5,682,820 - -1.1
5,684,201 - -2.3
5,684,232 - -1.5
5,685,010 + -3.3
5,685,012 + -2.0
5,685,012 + +2.0
5,685,037 + -3.3

Or see this region's nucleotide sequence