Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4102 and BT4103 are separated by 15 nucleotides BT4103 and BT4104 are separated by 114 nucleotides BT4104 and BT4105 are separated by 370 nucleotides
BT4102: BT4102 - putative Sec-independent protein translocase (NCBI ptt file), at 5,362,366 to 5,362,587
BT4102
BT4103: BT4103 - putative Sec-independent protein translocase (NCBI ptt file), at 5,362,603 to 5,363,424
BT4103
BT4104: BT4104 - DNA helicase (NCBI ptt file), at 5,363,539 to 5,366,913
BT4104
BT4105: BT4105 - D-galacturonate transporter ExuT (from data) , at 5,367,284 to 5,368,771
BT4105
Position (kb)
5363
5364
5365
5366
5367 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5362.718 kb on + strand, within BT4103 at 5362.760 kb on + strand, within BT4103 at 5362.761 kb on - strand, within BT4103 at 5362.763 kb on + strand, within BT4103 at 5362.764 kb on - strand, within BT4103 at 5362.863 kb on - strand, within BT4103 at 5362.866 kb on + strand, within BT4103 at 5363.040 kb on - strand, within BT4103 at 5363.119 kb on - strand, within BT4103 at 5363.125 kb on - strand, within BT4103 at 5363.131 kb on - strand, within BT4103 at 5363.231 kb on - strand, within BT4103 at 5363.326 kb on + strand, within BT4103 at 5363.347 kb on - strand at 5363.439 kb on - strand at 5363.477 kb on + strand at 5363.478 kb on - strand at 5363.661 kb on + strand at 5363.661 kb on + strand at 5363.760 kb on + strand at 5363.761 kb on - strand at 5363.924 kb on - strand, within BT4104 at 5363.924 kb on - strand, within BT4104 at 5363.925 kb on + strand, within BT4104 at 5363.995 kb on + strand, within BT4104 at 5364.001 kb on + strand, within BT4104 at 5364.054 kb on + strand, within BT4104 at 5364.198 kb on + strand, within BT4104 at 5364.250 kb on + strand, within BT4104 at 5364.335 kb on + strand, within BT4104 at 5364.336 kb on - strand, within BT4104 at 5364.369 kb on + strand, within BT4104 at 5364.560 kb on - strand, within BT4104 at 5364.561 kb on + strand, within BT4104 at 5364.561 kb on + strand, within BT4104 at 5364.561 kb on + strand, within BT4104 at 5364.561 kb on + strand, within BT4104 at 5364.562 kb on - strand, within BT4104 at 5364.562 kb on - strand, within BT4104 at 5364.599 kb on - strand, within BT4104 at 5364.599 kb on - strand, within BT4104 at 5364.600 kb on + strand, within BT4104 at 5364.601 kb on - strand, within BT4104 at 5364.601 kb on - strand, within BT4104 at 5364.766 kb on + strand, within BT4104 at 5364.776 kb on - strand, within BT4104 at 5364.776 kb on - strand, within BT4104 at 5364.776 kb on - strand, within BT4104 at 5364.900 kb on + strand, within BT4104 at 5364.900 kb on + strand, within BT4104 at 5364.900 kb on + strand, within BT4104 at 5364.901 kb on - strand, within BT4104 at 5364.977 kb on - strand, within BT4104 at 5364.990 kb on + strand, within BT4104 at 5364.990 kb on + strand, within BT4104 at 5365.048 kb on + strand, within BT4104 at 5365.057 kb on + strand, within BT4104 at 5365.059 kb on + strand, within BT4104 at 5365.119 kb on + strand, within BT4104 at 5365.119 kb on + strand, within BT4104 at 5365.136 kb on + strand, within BT4104 at 5365.184 kb on - strand, within BT4104 at 5365.224 kb on + strand, within BT4104 at 5365.452 kb on - strand, within BT4104 at 5365.477 kb on - strand, within BT4104 at 5365.477 kb on - strand, within BT4104 at 5365.498 kb on - strand, within BT4104 at 5365.500 kb on + strand, within BT4104 at 5365.528 kb on + strand, within BT4104 at 5365.529 kb on - strand, within BT4104 at 5365.750 kb on - strand, within BT4104 at 5365.783 kb on + strand, within BT4104 at 5365.789 kb on - strand, within BT4104 at 5365.813 kb on - strand, within BT4104 at 5365.923 kb on + strand, within BT4104 at 5365.965 kb on + strand, within BT4104 at 5365.966 kb on - strand, within BT4104 at 5365.967 kb on + strand, within BT4104 at 5365.989 kb on + strand, within BT4104 at 5366.148 kb on + strand, within BT4104 at 5366.195 kb on + strand, within BT4104 at 5366.196 kb on - strand, within BT4104 at 5366.196 kb on - strand, within BT4104 at 5366.295 kb on + strand, within BT4104 at 5366.299 kb on + strand, within BT4104 at 5366.300 kb on - strand, within BT4104 at 5366.304 kb on + strand, within BT4104 at 5366.325 kb on + strand, within BT4104 at 5366.326 kb on - strand, within BT4104 at 5366.326 kb on - strand, within BT4104 at 5366.366 kb on - strand, within BT4104 at 5366.530 kb on - strand, within BT4104 at 5366.577 kb on + strand at 5366.578 kb on - strand at 5366.619 kb on - strand at 5366.651 kb on + strand at 5366.652 kb on - strand at 5366.722 kb on + strand at 5366.729 kb on - strand at 5367.071 kb on - strand at 5367.078 kb on - strand at 5367.081 kb on - strand at 5367.208 kb on + strand at 5367.209 kb on - strand at 5367.271 kb on + strand at 5367.276 kb on - strand at 5367.277 kb on + strand at 5367.374 kb on + strand at 5367.507 kb on + strand, within BT4105 at 5367.507 kb on + strand, within BT4105 at 5367.508 kb on - strand, within BT4105 at 5367.510 kb on - strand, within BT4105 at 5367.510 kb on - strand, within BT4105 at 5367.521 kb on - strand, within BT4105 at 5367.583 kb on + strand, within BT4105 at 5367.584 kb on - strand, within BT4105 at 5367.610 kb on + strand, within BT4105 at 5367.610 kb on + strand, within BT4105 at 5367.617 kb on + strand, within BT4105 at 5367.632 kb on - strand, within BT4105 at 5367.725 kb on + strand, within BT4105 at 5367.766 kb on + strand, within BT4105 at 5367.766 kb on + strand, within BT4105 at 5367.808 kb on + strand, within BT4105 at 5367.808 kb on + strand, within BT4105 at 5367.829 kb on - strand, within BT4105 at 5367.829 kb on - strand, within BT4105
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 5,362,718 + BT4103 0.14 -0.2 5,362,760 + BT4103 0.19 -4.0 5,362,761 - BT4103 0.19 -4.0 5,362,763 + BT4103 0.19 +1.6 5,362,764 - BT4103 0.20 -1.5 5,362,863 - BT4103 0.32 +0.0 5,362,866 + BT4103 0.32 +0.0 5,363,040 - BT4103 0.53 -3.3 5,363,119 - BT4103 0.63 +0.0 5,363,125 - BT4103 0.64 +1.0 5,363,131 - BT4103 0.64 +0.0 5,363,231 - BT4103 0.76 -1.0 5,363,326 + BT4103 0.88 +0.0 5,363,347 - +0.0 5,363,439 - -3.0 5,363,477 + -1.3 5,363,478 - -1.0 5,363,661 + +2.5 5,363,661 + -1.5 5,363,760 + -2.0 5,363,761 - +2.6 5,363,924 - BT4104 0.11 -3.1 5,363,924 - BT4104 0.11 +1.3 5,363,925 + BT4104 0.11 -1.9 5,363,995 + BT4104 0.14 -3.1 5,364,001 + BT4104 0.14 -4.4 5,364,054 + BT4104 0.15 -1.0 5,364,198 + BT4104 0.20 -2.5 5,364,250 + BT4104 0.21 +0.6 5,364,335 + BT4104 0.24 +0.0 5,364,336 - BT4104 0.24 +0.0 5,364,369 + BT4104 0.25 +1.2 5,364,560 - BT4104 0.30 -3.4 5,364,561 + BT4104 0.30 +1.0 5,364,561 + BT4104 0.30 -1.3 5,364,561 + BT4104 0.30 -2.4 5,364,561 + BT4104 0.30 +0.0 5,364,562 - BT4104 0.30 -3.5 5,364,562 - BT4104 0.30 +1.9 5,364,599 - BT4104 0.31 -1.0 5,364,599 - BT4104 0.31 +1.0 5,364,600 + BT4104 0.31 +0.0 5,364,601 - BT4104 0.31 -3.1 5,364,601 - BT4104 0.31 +0.0 5,364,766 + BT4104 0.36 -1.9 5,364,776 - BT4104 0.37 -1.4 5,364,776 - BT4104 0.37 -2.5 5,364,776 - BT4104 0.37 -4.5 5,364,900 + BT4104 0.40 -1.4 5,364,900 + BT4104 0.40 +3.0 5,364,900 + BT4104 0.40 +0.0 5,364,901 - BT4104 0.40 -3.1 5,364,977 - BT4104 0.43 -3.0 5,364,990 + BT4104 0.43 -0.4 5,364,990 + BT4104 0.43 -1.9 5,365,048 + BT4104 0.45 +0.0 5,365,057 + BT4104 0.45 -4.2 5,365,059 + BT4104 0.45 -2.5 5,365,119 + BT4104 0.47 -3.3 5,365,119 + BT4104 0.47 -4.5 5,365,136 + BT4104 0.47 -1.5 5,365,184 - BT4104 0.49 -3.8 5,365,224 + BT4104 0.50 -2.3 5,365,452 - BT4104 0.57 -2.9 5,365,477 - BT4104 0.57 -3.1 5,365,477 - BT4104 0.57 -0.6 5,365,498 - BT4104 0.58 +0.5 5,365,500 + BT4104 0.58 -0.6 5,365,528 + BT4104 0.59 -3.0 5,365,529 - BT4104 0.59 -2.5 5,365,750 - BT4104 0.66 -2.3 5,365,783 + BT4104 0.66 -4.2 5,365,789 - BT4104 0.67 +1.8 5,365,813 - BT4104 0.67 -3.5 5,365,923 + BT4104 0.71 +0.0 5,365,965 + BT4104 0.72 +0.6 5,365,966 - BT4104 0.72 -2.4 5,365,967 + BT4104 0.72 -3.8 5,365,989 + BT4104 0.73 -1.5 5,366,148 + BT4104 0.77 -2.5 5,366,195 + BT4104 0.79 -2.3 5,366,196 - BT4104 0.79 -2.3 5,366,196 - BT4104 0.79 -4.3 5,366,295 + BT4104 0.82 -0.6 5,366,299 + BT4104 0.82 +0.0 5,366,300 - BT4104 0.82 +1.5 5,366,304 + BT4104 0.82 -2.3 5,366,325 + BT4104 0.83 +0.5 5,366,326 - BT4104 0.83 -1.3 5,366,326 - BT4104 0.83 +0.6 5,366,366 - BT4104 0.84 +0.4 5,366,530 - BT4104 0.89 -3.4 5,366,577 + -1.0 5,366,578 - -3.5 5,366,619 - -0.5 5,366,651 + +1.5 5,366,652 - +2.8 5,366,722 + +2.4 5,366,729 - +0.0 5,367,071 - +0.4 5,367,078 - -1.0 5,367,081 - +2.6 5,367,208 + -1.8 5,367,209 - +0.9 5,367,271 + -1.3 5,367,276 - -2.1 5,367,277 + +0.6 5,367,374 + +0.6 5,367,507 + BT4105 0.15 -1.5 5,367,507 + BT4105 0.15 +3.0 5,367,508 - BT4105 0.15 -2.0 5,367,510 - BT4105 0.15 -2.0 5,367,510 - BT4105 0.15 -1.8 5,367,521 - BT4105 0.16 -1.0 5,367,583 + BT4105 0.20 +1.0 5,367,584 - BT4105 0.20 -2.5 5,367,610 + BT4105 0.22 -1.0 5,367,610 + BT4105 0.22 -0.5 5,367,617 + BT4105 0.22 -1.0 5,367,632 - BT4105 0.23 +2.4 5,367,725 + BT4105 0.30 -2.3 5,367,766 + BT4105 0.32 +1.6 5,367,766 + BT4105 0.32 +2.6 5,367,808 + BT4105 0.35 -3.8 5,367,808 + BT4105 0.35 -1.2 5,367,829 - BT4105 0.37 +2.4 5,367,829 - BT4105 0.37 +0.0
Or see this region's nucleotide sequence