Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT_4101

Experiment: BHIS with Trimethoprim 0.00625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4100 and BT_4101 overlap by 25 nucleotidesBT_4101 and BT4102 are separated by 208 nucleotidesBT4102 and BT4103 are separated by 15 nucleotides BT4100: BT4100 - hypothetical protein (NCBI ptt file), at 5,358,643 to 5,359,632 BT4100 BT_4101: BT_4101 - putative bifunctional UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase/alanine racemase (RefSeq), at 5,359,608 to 5,362,157 _4101 BT4102: BT4102 - putative Sec-independent protein translocase (NCBI ptt file), at 5,362,366 to 5,362,587 BT4102 BT4103: BT4103 - putative Sec-independent protein translocase (NCBI ptt file), at 5,362,603 to 5,363,424 BT4103 Position (kb) 5359 5360 5361 5362 5363Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 5358.640 kb on + strandat 5358.640 kb on + strandat 5358.641 kb on - strandat 5358.642 kb on + strandat 5358.643 kb on - strandat 5358.662 kb on + strandat 5358.668 kb on + strandat 5358.853 kb on + strand, within BT4100at 5358.869 kb on + strand, within BT4100at 5358.875 kb on + strand, within BT4100at 5358.875 kb on + strand, within BT4100at 5358.876 kb on - strand, within BT4100at 5358.877 kb on + strand, within BT4100at 5358.878 kb on - strand, within BT4100at 5358.880 kb on - strand, within BT4100at 5358.941 kb on + strand, within BT4100at 5358.942 kb on - strand, within BT4100at 5359.027 kb on + strand, within BT4100at 5359.027 kb on + strand, within BT4100at 5359.027 kb on + strand, within BT4100at 5359.028 kb on - strand, within BT4100at 5359.031 kb on + strand, within BT4100at 5359.032 kb on - strand, within BT4100at 5359.075 kb on - strand, within BT4100at 5359.138 kb on - strand, within BT4100at 5359.155 kb on + strand, within BT4100at 5359.215 kb on - strand, within BT4100at 5359.256 kb on + strand, within BT4100at 5359.256 kb on + strand, within BT4100at 5359.306 kb on + strand, within BT4100at 5359.318 kb on + strand, within BT4100at 5359.471 kb on - strand, within BT4100at 5359.473 kb on + strand, within BT4100at 5359.477 kb on - strand, within BT4100at 5359.520 kb on - strand, within BT4100at 5362.182 kb on - strandat 5362.184 kb on - strandat 5362.184 kb on - strandat 5362.212 kb on + strandat 5362.213 kb on - strandat 5362.229 kb on + strandat 5362.230 kb on - strandat 5362.230 kb on - strandat 5362.353 kb on - strandat 5362.353 kb on - strandat 5362.365 kb on + strandat 5362.366 kb on - strandat 5362.376 kb on + strandat 5362.420 kb on - strand, within BT4102at 5362.481 kb on + strand, within BT4102at 5362.486 kb on - strand, within BT4102at 5362.493 kb on + strand, within BT4102at 5362.718 kb on + strand, within BT4103at 5362.760 kb on + strand, within BT4103at 5362.761 kb on - strand, within BT4103at 5362.763 kb on + strand, within BT4103at 5362.764 kb on - strand, within BT4103at 5362.863 kb on - strand, within BT4103at 5362.866 kb on + strand, within BT4103at 5363.040 kb on - strand, within BT4103at 5363.119 kb on - strand, within BT4103at 5363.125 kb on - strand, within BT4103at 5363.131 kb on - strand, within BT4103

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM
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5,358,640 + -2.3
5,358,640 + -0.1
5,358,641 - -3.5
5,358,642 + +1.7
5,358,643 - +1.3
5,358,662 + -2.0
5,358,668 + -4.2
5,358,853 + BT4100 0.21 -2.1
5,358,869 + BT4100 0.23 -3.0
5,358,875 + BT4100 0.23 +0.0
5,358,875 + BT4100 0.23 +0.6
5,358,876 - BT4100 0.24 +2.9
5,358,877 + BT4100 0.24 -2.4
5,358,878 - BT4100 0.24 -1.7
5,358,880 - BT4100 0.24 -3.0
5,358,941 + BT4100 0.30 -1.3
5,358,942 - BT4100 0.30 -1.7
5,359,027 + BT4100 0.39 -2.8
5,359,027 + BT4100 0.39 +1.2
5,359,027 + BT4100 0.39 -2.0
5,359,028 - BT4100 0.39 -0.9
5,359,031 + BT4100 0.39 -2.1
5,359,032 - BT4100 0.39 +0.5
5,359,075 - BT4100 0.44 -1.5
5,359,138 - BT4100 0.50 +2.5
5,359,155 + BT4100 0.52 -1.5
5,359,215 - BT4100 0.58 +1.0
5,359,256 + BT4100 0.62 -1.5
5,359,256 + BT4100 0.62 -1.3
5,359,306 + BT4100 0.67 -3.5
5,359,318 + BT4100 0.68 +0.7
5,359,471 - BT4100 0.84 +0.0
5,359,473 + BT4100 0.84 -1.8
5,359,477 - BT4100 0.84 -2.5
5,359,520 - BT4100 0.89 +2.0
5,362,182 - -1.0
5,362,184 - -2.2
5,362,184 - +1.2
5,362,212 + -2.8
5,362,213 - -3.9
5,362,229 + -1.2
5,362,230 - +2.9
5,362,230 - -1.7
5,362,353 - -3.5
5,362,353 - +1.0
5,362,365 + +3.2
5,362,366 - -2.0
5,362,376 + -1.0
5,362,420 - BT4102 0.24 +1.0
5,362,481 + BT4102 0.52 +0.6
5,362,486 - BT4102 0.54 -1.5
5,362,493 + BT4102 0.57 +0.3
5,362,718 + BT4103 0.14 -0.2
5,362,760 + BT4103 0.19 -4.0
5,362,761 - BT4103 0.19 -4.0
5,362,763 + BT4103 0.19 +1.6
5,362,764 - BT4103 0.20 -1.5
5,362,863 - BT4103 0.32 +0.0
5,362,866 + BT4103 0.32 +0.0
5,363,040 - BT4103 0.53 -3.3
5,363,119 - BT4103 0.63 +0.0
5,363,125 - BT4103 0.64 +1.0
5,363,131 - BT4103 0.64 +0.0

Or see this region's nucleotide sequence