Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4100 and BT_4101 overlap by 25 nucleotides BT_4101 and BT4102 are separated by 208 nucleotides BT4102 and BT4103 are separated by 15 nucleotides
BT4100: BT4100 - hypothetical protein (NCBI ptt file), at 5,358,643 to 5,359,632
BT4100
BT_4101: BT_4101 - putative bifunctional UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase/alanine racemase (RefSeq), at 5,359,608 to 5,362,157
_4101
BT4102: BT4102 - putative Sec-independent protein translocase (NCBI ptt file), at 5,362,366 to 5,362,587
BT4102
BT4103: BT4103 - putative Sec-independent protein translocase (NCBI ptt file), at 5,362,603 to 5,363,424
BT4103
Position (kb)
5359
5360
5361
5362
5363 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5358.640 kb on + strand at 5358.640 kb on + strand at 5358.641 kb on - strand at 5358.642 kb on + strand at 5358.643 kb on - strand at 5358.662 kb on + strand at 5358.668 kb on + strand at 5358.853 kb on + strand, within BT4100 at 5358.869 kb on + strand, within BT4100 at 5358.875 kb on + strand, within BT4100 at 5358.875 kb on + strand, within BT4100 at 5358.876 kb on - strand, within BT4100 at 5358.877 kb on + strand, within BT4100 at 5358.878 kb on - strand, within BT4100 at 5358.880 kb on - strand, within BT4100 at 5358.941 kb on + strand, within BT4100 at 5358.942 kb on - strand, within BT4100 at 5359.027 kb on + strand, within BT4100 at 5359.027 kb on + strand, within BT4100 at 5359.027 kb on + strand, within BT4100 at 5359.028 kb on - strand, within BT4100 at 5359.031 kb on + strand, within BT4100 at 5359.032 kb on - strand, within BT4100 at 5359.075 kb on - strand, within BT4100 at 5359.138 kb on - strand, within BT4100 at 5359.155 kb on + strand, within BT4100 at 5359.215 kb on - strand, within BT4100 at 5359.256 kb on + strand, within BT4100 at 5359.256 kb on + strand, within BT4100 at 5359.306 kb on + strand, within BT4100 at 5359.318 kb on + strand, within BT4100 at 5359.471 kb on - strand, within BT4100 at 5359.473 kb on + strand, within BT4100 at 5359.477 kb on - strand, within BT4100 at 5359.520 kb on - strand, within BT4100 at 5362.182 kb on - strand at 5362.184 kb on - strand at 5362.184 kb on - strand at 5362.212 kb on + strand at 5362.213 kb on - strand at 5362.229 kb on + strand at 5362.230 kb on - strand at 5362.230 kb on - strand at 5362.353 kb on - strand at 5362.353 kb on - strand at 5362.365 kb on + strand at 5362.366 kb on - strand at 5362.376 kb on + strand at 5362.420 kb on - strand, within BT4102 at 5362.481 kb on + strand, within BT4102 at 5362.486 kb on - strand, within BT4102 at 5362.493 kb on + strand, within BT4102 at 5362.718 kb on + strand, within BT4103 at 5362.760 kb on + strand, within BT4103 at 5362.761 kb on - strand, within BT4103 at 5362.763 kb on + strand, within BT4103 at 5362.764 kb on - strand, within BT4103 at 5362.863 kb on - strand, within BT4103 at 5362.866 kb on + strand, within BT4103 at 5363.040 kb on - strand, within BT4103 at 5363.119 kb on - strand, within BT4103 at 5363.125 kb on - strand, within BT4103 at 5363.131 kb on - strand, within BT4103
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 5,358,640 + -2.3 5,358,640 + -0.1 5,358,641 - -3.5 5,358,642 + +1.7 5,358,643 - +1.3 5,358,662 + -2.0 5,358,668 + -4.2 5,358,853 + BT4100 0.21 -2.1 5,358,869 + BT4100 0.23 -3.0 5,358,875 + BT4100 0.23 +0.0 5,358,875 + BT4100 0.23 +0.6 5,358,876 - BT4100 0.24 +2.9 5,358,877 + BT4100 0.24 -2.4 5,358,878 - BT4100 0.24 -1.7 5,358,880 - BT4100 0.24 -3.0 5,358,941 + BT4100 0.30 -1.3 5,358,942 - BT4100 0.30 -1.7 5,359,027 + BT4100 0.39 -2.8 5,359,027 + BT4100 0.39 +1.2 5,359,027 + BT4100 0.39 -2.0 5,359,028 - BT4100 0.39 -0.9 5,359,031 + BT4100 0.39 -2.1 5,359,032 - BT4100 0.39 +0.5 5,359,075 - BT4100 0.44 -1.5 5,359,138 - BT4100 0.50 +2.5 5,359,155 + BT4100 0.52 -1.5 5,359,215 - BT4100 0.58 +1.0 5,359,256 + BT4100 0.62 -1.5 5,359,256 + BT4100 0.62 -1.3 5,359,306 + BT4100 0.67 -3.5 5,359,318 + BT4100 0.68 +0.7 5,359,471 - BT4100 0.84 +0.0 5,359,473 + BT4100 0.84 -1.8 5,359,477 - BT4100 0.84 -2.5 5,359,520 - BT4100 0.89 +2.0 5,362,182 - -1.0 5,362,184 - -2.2 5,362,184 - +1.2 5,362,212 + -2.8 5,362,213 - -3.9 5,362,229 + -1.2 5,362,230 - +2.9 5,362,230 - -1.7 5,362,353 - -3.5 5,362,353 - +1.0 5,362,365 + +3.2 5,362,366 - -2.0 5,362,376 + -1.0 5,362,420 - BT4102 0.24 +1.0 5,362,481 + BT4102 0.52 +0.6 5,362,486 - BT4102 0.54 -1.5 5,362,493 + BT4102 0.57 +0.3 5,362,718 + BT4103 0.14 -0.2 5,362,760 + BT4103 0.19 -4.0 5,362,761 - BT4103 0.19 -4.0 5,362,763 + BT4103 0.19 +1.6 5,362,764 - BT4103 0.20 -1.5 5,362,863 - BT4103 0.32 +0.0 5,362,866 + BT4103 0.32 +0.0 5,363,040 - BT4103 0.53 -3.3 5,363,119 - BT4103 0.63 +0.0 5,363,125 - BT4103 0.64 +1.0 5,363,131 - BT4103 0.64 +0.0
Or see this region's nucleotide sequence