Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3890 and BT3891 are separated by 11 nucleotides BT3891 and BT3892 are separated by 51 nucleotides BT3892 and BT3893 are separated by 40 nucleotides BT3893 and BT3894 are separated by 26 nucleotides
BT3890: BT3890 - putative exodeoxyribonuclease VII large subunit (NCBI ptt file), at 5,061,369 to 5,062,622
BT3890
BT3891: BT3891 - hypothetical protein (NCBI ptt file), at 5,062,634 to 5,062,840
BT3891
BT3892: BT3892 - branched-chain amino acid aminotransferase (NCBI ptt file), at 5,062,892 to 5,063,911
BT3892
BT3893: BT3893 - conserved hypothetical protein (NCBI ptt file), at 5,063,952 to 5,064,575
BT3893
BT3894: BT3894 - putative methyltransferase (NCBI ptt file), at 5,064,602 to 5,065,444
BT3894
Position (kb)
5062
5063
5064 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 5061.906 kb on + strand, within BT3890 at 5062.006 kb on + strand, within BT3890 at 5062.059 kb on - strand, within BT3890 at 5062.063 kb on - strand, within BT3890 at 5062.063 kb on - strand, within BT3890 at 5062.264 kb on - strand, within BT3890 at 5062.488 kb on - strand, within BT3890 at 5062.488 kb on - strand, within BT3890 at 5062.553 kb on - strand at 5062.608 kb on + strand at 5062.609 kb on - strand at 5062.609 kb on - strand at 5062.609 kb on - strand at 5062.647 kb on + strand at 5062.655 kb on + strand, within BT3891 at 5062.656 kb on - strand, within BT3891 at 5062.686 kb on + strand at 5062.878 kb on + strand at 5063.909 kb on + strand at 5063.921 kb on - strand at 5063.924 kb on + strand at 5063.924 kb on + strand at 5063.926 kb on + strand at 5063.927 kb on - strand at 5063.936 kb on + strand at 5063.948 kb on + strand at 5063.952 kb on - strand at 5064.006 kb on - strand at 5064.006 kb on - strand at 5064.015 kb on - strand, within BT3893 at 5064.040 kb on - strand, within BT3893 at 5064.244 kb on - strand, within BT3893 at 5064.262 kb on + strand, within BT3893 at 5064.288 kb on - strand, within BT3893 at 5064.294 kb on + strand, within BT3893 at 5064.294 kb on + strand, within BT3893 at 5064.295 kb on - strand, within BT3893 at 5064.318 kb on - strand, within BT3893 at 5064.493 kb on + strand, within BT3893 at 5064.511 kb on - strand, within BT3893 at 5064.543 kb on - strand at 5064.553 kb on + strand at 5064.573 kb on + strand at 5064.574 kb on - strand at 5064.577 kb on - strand at 5064.577 kb on - strand at 5064.585 kb on - strand at 5064.596 kb on + strand at 5064.631 kb on + strand at 5064.656 kb on - strand at 5064.697 kb on + strand, within BT3894 at 5064.766 kb on + strand, within BT3894 at 5064.860 kb on + strand, within BT3894 at 5064.860 kb on + strand, within BT3894 at 5064.901 kb on + strand, within BT3894 at 5064.901 kb on + strand, within BT3894 at 5064.903 kb on + strand, within BT3894 at 5064.904 kb on - strand, within BT3894
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 5,061,906 + BT3890 0.43 -3.4 5,062,006 + BT3890 0.51 +1.6 5,062,059 - BT3890 0.55 -1.6 5,062,063 - BT3890 0.55 -1.0 5,062,063 - BT3890 0.55 -1.2 5,062,264 - BT3890 0.71 +2.4 5,062,488 - BT3890 0.89 +0.5 5,062,488 - BT3890 0.89 -1.2 5,062,553 - -2.3 5,062,608 + -0.3 5,062,609 - +0.5 5,062,609 - -2.0 5,062,609 - +1.6 5,062,647 + -1.7 5,062,655 + BT3891 0.10 -1.2 5,062,656 - BT3891 0.11 -2.8 5,062,686 + +0.0 5,062,878 + -0.1 5,063,909 + +1.5 5,063,921 - -0.1 5,063,924 + -1.5 5,063,924 + +0.6 5,063,926 + -0.5 5,063,927 - -0.2 5,063,936 + -1.5 5,063,948 + +3.2 5,063,952 - -0.6 5,064,006 - -0.3 5,064,006 - +1.0 5,064,015 - BT3893 0.10 +0.0 5,064,040 - BT3893 0.14 +1.3 5,064,244 - BT3893 0.47 -1.6 5,064,262 + BT3893 0.50 -1.1 5,064,288 - BT3893 0.54 -0.8 5,064,294 + BT3893 0.55 -0.3 5,064,294 + BT3893 0.55 -0.7 5,064,295 - BT3893 0.55 -0.7 5,064,318 - BT3893 0.59 +1.3 5,064,493 + BT3893 0.87 -0.5 5,064,511 - BT3893 0.90 -3.3 5,064,543 - +1.6 5,064,553 + -3.1 5,064,573 + +0.8 5,064,574 - -0.5 5,064,577 - -1.0 5,064,577 - -2.5 5,064,585 - +1.0 5,064,596 + +0.5 5,064,631 + -1.6 5,064,656 - +0.0 5,064,697 + BT3894 0.11 -1.0 5,064,766 + BT3894 0.19 +4.1 5,064,860 + BT3894 0.31 -2.3 5,064,860 + BT3894 0.31 -2.1 5,064,901 + BT3894 0.35 +2.4 5,064,901 + BT3894 0.35 +1.0 5,064,903 + BT3894 0.36 -3.8 5,064,904 - BT3894 0.36 -1.5
Or see this region's nucleotide sequence