Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3755 and BT3756 are separated by 7 nucleotides BT3756 and BT3757 are separated by 39 nucleotides BT3757 and BT3758 are separated by 135 nucleotides
BT3755: BT3755 - acetyl-coenzyme A synthetase (NCBI ptt file), at 4,875,308 to 4,876,963
BT3755
BT3756: BT3756 - transcriptional regulator (NCBI ptt file), at 4,876,971 to 4,877,525
BT3756
BT3757: BT3757 - pyrroline-5-carboxylate reductase (EC 1.5.1.2) (from data) , at 4,877,565 to 4,878,338
BT3757
BT3758: BT3758 - N-succinylornithine aminotransferase (EC 2.6.1.81) (from data) , at 4,878,474 to 4,879,595
BT3758
Position (kb)
4876
4877
4878 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4876.041 kb on + strand, within BT3755 at 4876.041 kb on + strand, within BT3755 at 4876.042 kb on - strand, within BT3755 at 4876.042 kb on - strand, within BT3755 at 4876.042 kb on - strand, within BT3755 at 4876.042 kb on - strand, within BT3755 at 4876.045 kb on + strand, within BT3755 at 4876.045 kb on + strand, within BT3755 at 4876.045 kb on + strand, within BT3755 at 4876.046 kb on - strand, within BT3755 at 4876.162 kb on - strand, within BT3755 at 4876.312 kb on + strand, within BT3755 at 4876.312 kb on + strand, within BT3755 at 4876.313 kb on - strand, within BT3755 at 4876.326 kb on - strand, within BT3755 at 4876.333 kb on + strand, within BT3755 at 4876.334 kb on - strand, within BT3755 at 4876.334 kb on - strand, within BT3755 at 4876.387 kb on + strand, within BT3755 at 4876.588 kb on - strand, within BT3755 at 4876.588 kb on - strand, within BT3755 at 4876.595 kb on - strand, within BT3755 at 4876.646 kb on - strand, within BT3755 at 4876.737 kb on + strand, within BT3755 at 4876.856 kb on + strand at 4876.857 kb on - strand at 4876.859 kb on + strand at 4876.896 kb on - strand at 4876.937 kb on - strand at 4876.937 kb on - strand at 4876.937 kb on - strand at 4876.958 kb on + strand at 4876.958 kb on + strand at 4876.959 kb on - strand at 4876.977 kb on - strand at 4877.047 kb on + strand, within BT3756 at 4877.102 kb on + strand, within BT3756 at 4877.149 kb on + strand at 4877.355 kb on - strand, within BT3756 at 4877.362 kb on - strand, within BT3756 at 4877.375 kb on + strand, within BT3756 at 4877.375 kb on + strand, within BT3756 at 4877.376 kb on - strand, within BT3756 at 4877.417 kb on - strand, within BT3756 at 4877.430 kb on + strand, within BT3756 at 4877.463 kb on + strand, within BT3756 at 4877.463 kb on + strand, within BT3756 at 4877.473 kb on - strand at 4877.510 kb on + strand at 4877.566 kb on + strand at 4877.643 kb on - strand, within BT3757 at 4877.659 kb on + strand, within BT3757 at 4877.706 kb on + strand, within BT3757 at 4877.737 kb on - strand, within BT3757 at 4877.737 kb on - strand, within BT3757 at 4877.803 kb on + strand, within BT3757 at 4877.816 kb on - strand, within BT3757 at 4877.909 kb on + strand, within BT3757 at 4878.244 kb on - strand, within BT3757 at 4878.244 kb on - strand, within BT3757 at 4878.251 kb on + strand, within BT3757 at 4878.251 kb on + strand, within BT3757 at 4878.296 kb on + strand at 4878.326 kb on + strand at 4878.330 kb on + strand at 4878.358 kb on + strand at 4878.359 kb on - strand at 4878.365 kb on - strand at 4878.383 kb on - strand at 4878.414 kb on - strand at 4878.458 kb on - strand at 4878.476 kb on - strand at 4878.481 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 4,876,041 + BT3755 0.44 +2.9 4,876,041 + BT3755 0.44 -1.3 4,876,042 - BT3755 0.44 +0.3 4,876,042 - BT3755 0.44 -0.4 4,876,042 - BT3755 0.44 -1.3 4,876,042 - BT3755 0.44 -2.1 4,876,045 + BT3755 0.45 +0.4 4,876,045 + BT3755 0.45 +0.3 4,876,045 + BT3755 0.45 -0.0 4,876,046 - BT3755 0.45 -1.5 4,876,162 - BT3755 0.52 +1.5 4,876,312 + BT3755 0.61 -0.1 4,876,312 + BT3755 0.61 -1.7 4,876,313 - BT3755 0.61 -1.2 4,876,326 - BT3755 0.61 +1.1 4,876,333 + BT3755 0.62 -1.9 4,876,334 - BT3755 0.62 -1.3 4,876,334 - BT3755 0.62 -1.0 4,876,387 + BT3755 0.65 +0.5 4,876,588 - BT3755 0.77 +2.3 4,876,588 - BT3755 0.77 +0.5 4,876,595 - BT3755 0.78 -0.4 4,876,646 - BT3755 0.81 +1.3 4,876,737 + BT3755 0.86 +0.5 4,876,856 + -1.5 4,876,857 - +2.1 4,876,859 + -2.2 4,876,896 - +2.0 4,876,937 - +1.9 4,876,937 - +1.8 4,876,937 - -1.2 4,876,958 + -1.9 4,876,958 + +1.3 4,876,959 - -0.5 4,876,977 - -2.3 4,877,047 + BT3756 0.14 -3.3 4,877,102 + BT3756 0.24 -1.5 4,877,149 + -0.9 4,877,355 - BT3756 0.69 +1.1 4,877,362 - BT3756 0.70 -2.8 4,877,375 + BT3756 0.73 +1.4 4,877,375 + BT3756 0.73 -3.1 4,877,376 - BT3756 0.73 -1.5 4,877,417 - BT3756 0.80 -3.6 4,877,430 + BT3756 0.83 -4.6 4,877,463 + BT3756 0.89 -0.9 4,877,463 + BT3756 0.89 +0.7 4,877,473 - -3.1 4,877,510 + -1.4 4,877,566 + -1.9 4,877,643 - BT3757 0.10 -1.8 4,877,659 + BT3757 0.12 -2.5 4,877,706 + BT3757 0.18 -0.5 4,877,737 - BT3757 0.22 -1.2 4,877,737 - BT3757 0.22 -0.8 4,877,803 + BT3757 0.31 -2.8 4,877,816 - BT3757 0.32 -3.4 4,877,909 + BT3757 0.44 -2.4 4,878,244 - BT3757 0.88 -3.5 4,878,244 - BT3757 0.88 +1.1 4,878,251 + BT3757 0.89 -0.1 4,878,251 + BT3757 0.89 -2.3 4,878,296 + +0.4 4,878,326 + -1.8 4,878,330 + +0.8 4,878,358 + +3.0 4,878,359 - -1.5 4,878,365 - -0.6 4,878,383 - +1.5 4,878,414 - -1.8 4,878,458 - +2.2 4,878,476 - +3.4 4,878,481 - +1.4
Or see this region's nucleotide sequence