Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT3756

Experiment: BHIS with Trimethoprim 0.00625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT3755 and BT3756 are separated by 7 nucleotidesBT3756 and BT3757 are separated by 39 nucleotidesBT3757 and BT3758 are separated by 135 nucleotides BT3755: BT3755 - acetyl-coenzyme A synthetase (NCBI ptt file), at 4,875,308 to 4,876,963 BT3755 BT3756: BT3756 - transcriptional regulator (NCBI ptt file), at 4,876,971 to 4,877,525 BT3756 BT3757: BT3757 - pyrroline-5-carboxylate reductase (EC 1.5.1.2) (from data), at 4,877,565 to 4,878,338 BT3757 BT3758: BT3758 - N-succinylornithine aminotransferase (EC 2.6.1.81) (from data), at 4,878,474 to 4,879,595 BT3758 Position (kb) 4876 4877 4878Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 4876.041 kb on + strand, within BT3755at 4876.041 kb on + strand, within BT3755at 4876.042 kb on - strand, within BT3755at 4876.042 kb on - strand, within BT3755at 4876.042 kb on - strand, within BT3755at 4876.042 kb on - strand, within BT3755at 4876.045 kb on + strand, within BT3755at 4876.045 kb on + strand, within BT3755at 4876.045 kb on + strand, within BT3755at 4876.046 kb on - strand, within BT3755at 4876.162 kb on - strand, within BT3755at 4876.312 kb on + strand, within BT3755at 4876.312 kb on + strand, within BT3755at 4876.313 kb on - strand, within BT3755at 4876.326 kb on - strand, within BT3755at 4876.333 kb on + strand, within BT3755at 4876.334 kb on - strand, within BT3755at 4876.334 kb on - strand, within BT3755at 4876.387 kb on + strand, within BT3755at 4876.588 kb on - strand, within BT3755at 4876.588 kb on - strand, within BT3755at 4876.595 kb on - strand, within BT3755at 4876.646 kb on - strand, within BT3755at 4876.737 kb on + strand, within BT3755at 4876.856 kb on + strandat 4876.857 kb on - strandat 4876.859 kb on + strandat 4876.896 kb on - strandat 4876.937 kb on - strandat 4876.937 kb on - strandat 4876.937 kb on - strandat 4876.958 kb on + strandat 4876.958 kb on + strandat 4876.959 kb on - strandat 4876.977 kb on - strandat 4877.047 kb on + strand, within BT3756at 4877.102 kb on + strand, within BT3756at 4877.149 kb on + strandat 4877.355 kb on - strand, within BT3756at 4877.362 kb on - strand, within BT3756at 4877.375 kb on + strand, within BT3756at 4877.375 kb on + strand, within BT3756at 4877.376 kb on - strand, within BT3756at 4877.417 kb on - strand, within BT3756at 4877.430 kb on + strand, within BT3756at 4877.463 kb on + strand, within BT3756at 4877.463 kb on + strand, within BT3756at 4877.473 kb on - strandat 4877.510 kb on + strandat 4877.566 kb on + strandat 4877.643 kb on - strand, within BT3757at 4877.659 kb on + strand, within BT3757at 4877.706 kb on + strand, within BT3757at 4877.737 kb on - strand, within BT3757at 4877.737 kb on - strand, within BT3757at 4877.803 kb on + strand, within BT3757at 4877.816 kb on - strand, within BT3757at 4877.909 kb on + strand, within BT3757at 4878.244 kb on - strand, within BT3757at 4878.244 kb on - strand, within BT3757at 4878.251 kb on + strand, within BT3757at 4878.251 kb on + strand, within BT3757at 4878.296 kb on + strandat 4878.326 kb on + strandat 4878.330 kb on + strandat 4878.358 kb on + strandat 4878.359 kb on - strandat 4878.365 kb on - strandat 4878.383 kb on - strandat 4878.414 kb on - strandat 4878.458 kb on - strandat 4878.476 kb on - strandat 4878.481 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM
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4,876,041 + BT3755 0.44 +2.9
4,876,041 + BT3755 0.44 -1.3
4,876,042 - BT3755 0.44 +0.3
4,876,042 - BT3755 0.44 -0.4
4,876,042 - BT3755 0.44 -1.3
4,876,042 - BT3755 0.44 -2.1
4,876,045 + BT3755 0.45 +0.4
4,876,045 + BT3755 0.45 +0.3
4,876,045 + BT3755 0.45 -0.0
4,876,046 - BT3755 0.45 -1.5
4,876,162 - BT3755 0.52 +1.5
4,876,312 + BT3755 0.61 -0.1
4,876,312 + BT3755 0.61 -1.7
4,876,313 - BT3755 0.61 -1.2
4,876,326 - BT3755 0.61 +1.1
4,876,333 + BT3755 0.62 -1.9
4,876,334 - BT3755 0.62 -1.3
4,876,334 - BT3755 0.62 -1.0
4,876,387 + BT3755 0.65 +0.5
4,876,588 - BT3755 0.77 +2.3
4,876,588 - BT3755 0.77 +0.5
4,876,595 - BT3755 0.78 -0.4
4,876,646 - BT3755 0.81 +1.3
4,876,737 + BT3755 0.86 +0.5
4,876,856 + -1.5
4,876,857 - +2.1
4,876,859 + -2.2
4,876,896 - +2.0
4,876,937 - +1.9
4,876,937 - +1.8
4,876,937 - -1.2
4,876,958 + -1.9
4,876,958 + +1.3
4,876,959 - -0.5
4,876,977 - -2.3
4,877,047 + BT3756 0.14 -3.3
4,877,102 + BT3756 0.24 -1.5
4,877,149 + -0.9
4,877,355 - BT3756 0.69 +1.1
4,877,362 - BT3756 0.70 -2.8
4,877,375 + BT3756 0.73 +1.4
4,877,375 + BT3756 0.73 -3.1
4,877,376 - BT3756 0.73 -1.5
4,877,417 - BT3756 0.80 -3.6
4,877,430 + BT3756 0.83 -4.6
4,877,463 + BT3756 0.89 -0.9
4,877,463 + BT3756 0.89 +0.7
4,877,473 - -3.1
4,877,510 + -1.4
4,877,566 + -1.9
4,877,643 - BT3757 0.10 -1.8
4,877,659 + BT3757 0.12 -2.5
4,877,706 + BT3757 0.18 -0.5
4,877,737 - BT3757 0.22 -1.2
4,877,737 - BT3757 0.22 -0.8
4,877,803 + BT3757 0.31 -2.8
4,877,816 - BT3757 0.32 -3.4
4,877,909 + BT3757 0.44 -2.4
4,878,244 - BT3757 0.88 -3.5
4,878,244 - BT3757 0.88 +1.1
4,878,251 + BT3757 0.89 -0.1
4,878,251 + BT3757 0.89 -2.3
4,878,296 + +0.4
4,878,326 + -1.8
4,878,330 + +0.8
4,878,358 + +3.0
4,878,359 - -1.5
4,878,365 - -0.6
4,878,383 - +1.5
4,878,414 - -1.8
4,878,458 - +2.2
4,878,476 - +3.4
4,878,481 - +1.4

Or see this region's nucleotide sequence