Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT3729

Experiment: BHIS with Trimethoprim 0.00625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT3728 and BT3729 are separated by 71 nucleotidesBT3729 and BT3730 overlap by 4 nucleotidesBT3730 and BT3731 are separated by 77 nucleotides BT3728: BT3728 - riboflavin biosynthesis protein ribD (NCBI ptt file), at 4,838,863 to 4,839,882 BT3728 BT3729: BT3729 - putative protoporphyrinogen oxidase (NCBI ptt file), at 4,839,954 to 4,840,790 BT3729 BT3730: BT3730 - putative regulatory protein (NCBI ptt file), at 4,840,787 to 4,841,269 BT3730 BT3731: BT3731 - orotate phosphoribosyltransferase (NCBI ptt file), at 4,841,347 to 4,841,985 BT3731 Position (kb) 4839 4840 4841Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4839.919 kb on - strandat 4839.922 kb on - strandat 4840.075 kb on - strand, within BT3729at 4840.295 kb on - strand, within BT3729at 4840.295 kb on - strand, within BT3729at 4840.298 kb on + strand, within BT3729at 4840.300 kb on + strand, within BT3729at 4840.300 kb on + strand, within BT3729at 4840.301 kb on - strand, within BT3729at 4840.373 kb on + strand, within BT3729at 4840.395 kb on - strand, within BT3729at 4840.485 kb on + strand, within BT3729at 4840.493 kb on - strand, within BT3729at 4840.687 kb on - strand, within BT3729at 4840.702 kb on + strand, within BT3729at 4840.896 kb on + strand, within BT3730at 4840.987 kb on + strand, within BT3730at 4841.023 kb on - strand, within BT3730

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM
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4,839,919 - -4.1
4,839,922 - -3.6
4,840,075 - BT3729 0.14 -4.2
4,840,295 - BT3729 0.41 -0.9
4,840,295 - BT3729 0.41 -2.7
4,840,298 + BT3729 0.41 +0.1
4,840,300 + BT3729 0.41 -4.5
4,840,300 + BT3729 0.41 -1.2
4,840,301 - BT3729 0.41 +0.1
4,840,373 + BT3729 0.50 -2.2
4,840,395 - BT3729 0.53 -1.5
4,840,485 + BT3729 0.63 -3.2
4,840,493 - BT3729 0.64 -2.5
4,840,687 - BT3729 0.88 +0.1
4,840,702 + BT3729 0.89 -0.9
4,840,896 + BT3730 0.23 +1.1
4,840,987 + BT3730 0.41 -0.9
4,841,023 - BT3730 0.49 +2.1

Or see this region's nucleotide sequence