Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3639 and BT3640 are separated by 9 nucleotides BT3640 and BT3641 are separated by 202 nucleotides BT3641 and BT3642 overlap by 1 nucleotides
BT3639: BT3639 - ThiF family protein, putative dinucleotide-utilizing enzyme involved in molybdopterin and thiamine biosynthesis (NCBI ptt file), at 4,716,495 to 4,717,211
BT3639
BT3640: BT3640 - lipoprotein releasing system ATP-binding protein (NCBI ptt file), at 4,717,221 to 4,717,877
BT3640
BT3641: BT3641 - conserved hypothetical protein (NCBI ptt file), at 4,718,080 to 4,719,009
BT3641
BT3642: BT3642 - Na+-dependent transporter (NCBI ptt file), at 4,719,009 to 4,720,379
BT3642
Position (kb)
4718
4719
4720 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4717.157 kb on + strand at 4717.199 kb on + strand at 4717.217 kb on + strand at 4717.947 kb on - strand at 4717.950 kb on + strand at 4717.955 kb on - strand at 4717.973 kb on - strand at 4717.973 kb on - strand at 4718.060 kb on - strand at 4718.164 kb on + strand at 4718.222 kb on + strand, within BT3641 at 4718.222 kb on + strand, within BT3641 at 4718.223 kb on - strand, within BT3641 at 4718.223 kb on - strand at 4718.223 kb on - strand, within BT3641 at 4718.354 kb on + strand, within BT3641 at 4718.427 kb on + strand, within BT3641 at 4718.428 kb on - strand at 4718.439 kb on + strand, within BT3641 at 4718.463 kb on + strand, within BT3641 at 4718.486 kb on - strand, within BT3641 at 4718.495 kb on + strand, within BT3641 at 4718.512 kb on + strand, within BT3641 at 4718.516 kb on - strand, within BT3641 at 4718.532 kb on + strand, within BT3641 at 4718.563 kb on + strand, within BT3641 at 4718.563 kb on + strand, within BT3641 at 4718.563 kb on + strand, within BT3641 at 4718.563 kb on + strand, within BT3641 at 4718.563 kb on + strand, within BT3641 at 4718.567 kb on + strand, within BT3641 at 4718.608 kb on + strand, within BT3641 at 4718.780 kb on + strand, within BT3641 at 4718.781 kb on - strand, within BT3641 at 4718.844 kb on - strand, within BT3641 at 4718.850 kb on - strand, within BT3641 at 4718.930 kb on + strand at 4718.930 kb on + strand at 4718.930 kb on + strand at 4718.930 kb on + strand at 4718.931 kb on - strand at 4718.932 kb on + strand at 4718.932 kb on + strand at 4718.933 kb on - strand at 4718.938 kb on + strand at 4718.985 kb on + strand at 4719.011 kb on - strand at 4719.021 kb on - strand at 4719.039 kb on - strand at 4719.039 kb on - strand at 4719.070 kb on + strand at 4719.076 kb on - strand at 4719.078 kb on + strand at 4719.080 kb on + strand at 4719.080 kb on + strand at 4719.232 kb on - strand, within BT3642 at 4719.303 kb on + strand, within BT3642 at 4719.304 kb on - strand, within BT3642 at 4719.311 kb on + strand, within BT3642 at 4719.311 kb on + strand, within BT3642 at 4719.312 kb on - strand, within BT3642 at 4719.312 kb on - strand, within BT3642 at 4719.361 kb on - strand, within BT3642 at 4719.433 kb on - strand, within BT3642 at 4719.436 kb on + strand, within BT3642 at 4719.437 kb on - strand, within BT3642 at 4719.455 kb on + strand, within BT3642 at 4719.456 kb on - strand, within BT3642 at 4719.456 kb on - strand, within BT3642 at 4719.496 kb on - strand, within BT3642 at 4719.518 kb on + strand, within BT3642 at 4719.543 kb on + strand, within BT3642 at 4719.544 kb on - strand, within BT3642 at 4719.544 kb on - strand, within BT3642 at 4719.544 kb on - strand, within BT3642 at 4719.544 kb on - strand, within BT3642 at 4719.573 kb on - strand, within BT3642 at 4719.603 kb on - strand, within BT3642 at 4719.603 kb on - strand, within BT3642 at 4719.606 kb on + strand, within BT3642 at 4719.607 kb on - strand, within BT3642 at 4719.607 kb on - strand, within BT3642 at 4719.608 kb on + strand, within BT3642 at 4719.668 kb on - strand, within BT3642 at 4719.696 kb on - strand, within BT3642 at 4719.696 kb on - strand, within BT3642 at 4719.814 kb on - strand, within BT3642 at 4719.816 kb on + strand, within BT3642 at 4719.817 kb on - strand, within BT3642 at 4719.817 kb on - strand, within BT3642 at 4719.828 kb on + strand, within BT3642 at 4719.902 kb on + strand, within BT3642 at 4719.903 kb on - strand, within BT3642
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 4,717,157 + -1.5 4,717,199 + -1.9 4,717,217 + -2.2 4,717,947 - -0.1 4,717,950 + -1.5 4,717,955 - -0.9 4,717,973 - -0.5 4,717,973 - -1.2 4,718,060 - +1.8 4,718,164 + -2.0 4,718,222 + BT3641 0.15 +1.1 4,718,222 + BT3641 0.15 +1.1 4,718,223 - BT3641 0.15 +4.3 4,718,223 - +2.1 4,718,223 - BT3641 0.15 +2.6 4,718,354 + BT3641 0.29 +0.0 4,718,427 + BT3641 0.37 +3.6 4,718,428 - +0.1 4,718,439 + BT3641 0.39 +1.1 4,718,463 + BT3641 0.41 -0.9 4,718,486 - BT3641 0.44 +0.2 4,718,495 + BT3641 0.45 +0.1 4,718,512 + BT3641 0.46 +2.7 4,718,516 - BT3641 0.47 +0.1 4,718,532 + BT3641 0.49 +0.1 4,718,563 + BT3641 0.52 +0.1 4,718,563 + BT3641 0.52 +0.1 4,718,563 + BT3641 0.52 -0.7 4,718,563 + BT3641 0.52 -2.5 4,718,563 + BT3641 0.52 -1.1 4,718,567 + BT3641 0.52 +0.1 4,718,608 + BT3641 0.57 +1.1 4,718,780 + BT3641 0.75 -0.1 4,718,781 - BT3641 0.75 +0.1 4,718,844 - BT3641 0.82 +3.4 4,718,850 - BT3641 0.83 +3.1 4,718,930 + +0.1 4,718,930 + -1.1 4,718,930 + +4.4 4,718,930 + +2.0 4,718,931 - -0.9 4,718,932 + -0.3 4,718,932 + -1.1 4,718,933 - +0.1 4,718,938 + -2.9 4,718,985 + -2.1 4,719,011 - -1.1 4,719,021 - -0.5 4,719,039 - -0.8 4,719,039 - +1.4 4,719,070 + -2.5 4,719,076 - -1.0 4,719,078 + +2.9 4,719,080 + -2.7 4,719,080 + -1.8 4,719,232 - BT3642 0.16 -1.2 4,719,303 + BT3642 0.21 -0.2 4,719,304 - BT3642 0.22 +1.1 4,719,311 + BT3642 0.22 -0.9 4,719,311 + BT3642 0.22 -0.9 4,719,312 - BT3642 0.22 -3.4 4,719,312 - BT3642 0.22 +0.4 4,719,361 - BT3642 0.26 -1.2 4,719,433 - BT3642 0.31 -1.7 4,719,436 + BT3642 0.31 -2.5 4,719,437 - BT3642 0.31 +1.1 4,719,455 + BT3642 0.33 -0.9 4,719,456 - BT3642 0.33 +0.1 4,719,456 - BT3642 0.33 -4.1 4,719,496 - BT3642 0.36 +0.8 4,719,518 + BT3642 0.37 +1.1 4,719,543 + BT3642 0.39 +4.0 4,719,544 - BT3642 0.39 +0.1 4,719,544 - BT3642 0.39 -1.6 4,719,544 - BT3642 0.39 -0.5 4,719,544 - BT3642 0.39 +0.1 4,719,573 - BT3642 0.41 -1.5 4,719,603 - BT3642 0.43 -1.5 4,719,603 - BT3642 0.43 -1.1 4,719,606 + BT3642 0.44 -1.4 4,719,607 - BT3642 0.44 -0.7 4,719,607 - BT3642 0.44 +2.7 4,719,608 + BT3642 0.44 -1.3 4,719,668 - BT3642 0.48 -0.4 4,719,696 - BT3642 0.50 -0.6 4,719,696 - BT3642 0.50 -1.2 4,719,814 - BT3642 0.59 +2.1 4,719,816 + BT3642 0.59 -3.1 4,719,817 - BT3642 0.59 -0.8 4,719,817 - BT3642 0.59 -1.7 4,719,828 + BT3642 0.60 -2.5 4,719,902 + BT3642 0.65 +1.3 4,719,903 - BT3642 0.65 +0.1
Or see this region's nucleotide sequence