Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3324 and BT3325 are separated by 794 nucleotides BT3325 and BT3326 are separated by 86 nucleotides
BT3324: BT3324 - chondroitinase (chondroitin lyase) precursor (NCBI ptt file), at 4,269,304 to 4,272,348
BT3324
BT3325: BT3325 - hypothetical protein (NCBI ptt file), at 4,273,143 to 4,274,372
BT3325
BT3326: BT3326 - putative nifR3 family TIM-barrel enzyme with dihydrouridine synthase domain (NCBI ptt file), at 4,274,459 to 4,275,403
BT3326
Position (kb)
4273
4274
4275 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4272.154 kb on - strand at 4272.365 kb on - strand at 4272.401 kb on - strand at 4272.422 kb on - strand at 4272.425 kb on - strand at 4272.449 kb on + strand at 4272.450 kb on - strand at 4272.450 kb on - strand at 4272.526 kb on - strand at 4272.568 kb on + strand at 4272.695 kb on + strand at 4272.714 kb on + strand at 4272.739 kb on + strand at 4272.747 kb on - strand at 4272.747 kb on - strand at 4272.749 kb on + strand at 4272.749 kb on + strand at 4272.750 kb on - strand at 4272.903 kb on - strand at 4272.903 kb on - strand at 4272.910 kb on + strand at 4272.910 kb on + strand at 4272.920 kb on - strand at 4272.996 kb on - strand at 4273.023 kb on - strand at 4273.108 kb on - strand at 4273.172 kb on + strand at 4273.173 kb on - strand at 4273.209 kb on - strand at 4273.343 kb on - strand, within BT3325 at 4273.359 kb on + strand, within BT3325 at 4273.359 kb on + strand, within BT3325 at 4273.469 kb on - strand, within BT3325 at 4273.495 kb on - strand, within BT3325 at 4273.503 kb on - strand, within BT3325 at 4273.588 kb on + strand, within BT3325 at 4273.589 kb on - strand, within BT3325 at 4273.617 kb on - strand, within BT3325 at 4273.623 kb on - strand, within BT3325 at 4273.639 kb on + strand, within BT3325 at 4273.640 kb on - strand, within BT3325 at 4273.643 kb on + strand, within BT3325 at 4273.658 kb on + strand, within BT3325 at 4273.661 kb on + strand, within BT3325 at 4273.662 kb on - strand, within BT3325 at 4273.783 kb on + strand, within BT3325 at 4273.810 kb on - strand, within BT3325 at 4274.011 kb on - strand, within BT3325 at 4274.048 kb on - strand, within BT3325 at 4274.170 kb on + strand, within BT3325 at 4274.171 kb on - strand, within BT3325 at 4274.172 kb on + strand, within BT3325 at 4274.173 kb on - strand, within BT3325 at 4274.173 kb on - strand, within BT3325 at 4274.252 kb on - strand at 4274.252 kb on - strand at 4274.294 kb on - strand at 4274.296 kb on + strand at 4274.358 kb on - strand at 4274.460 kb on + strand at 4274.461 kb on - strand at 4274.461 kb on - strand at 4274.518 kb on + strand at 4274.562 kb on + strand, within BT3326 at 4274.592 kb on - strand, within BT3326 at 4274.615 kb on + strand, within BT3326 at 4274.619 kb on - strand, within BT3326 at 4274.619 kb on - strand, within BT3326 at 4274.821 kb on - strand, within BT3326 at 4275.165 kb on - strand, within BT3326 at 4275.288 kb on - strand, within BT3326 at 4275.300 kb on + strand, within BT3326 at 4275.301 kb on - strand, within BT3326 at 4275.304 kb on - strand, within BT3326
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 4,272,154 - -2.4 4,272,365 - -2.0 4,272,401 - +2.1 4,272,422 - +0.2 4,272,425 - +1.8 4,272,449 + +1.6 4,272,450 - -1.5 4,272,450 - -0.8 4,272,526 - +0.5 4,272,568 + +1.0 4,272,695 + -1.6 4,272,714 + +0.9 4,272,739 + +0.2 4,272,747 - +0.2 4,272,747 - -1.4 4,272,749 + +3.0 4,272,749 + -1.0 4,272,750 - -1.1 4,272,903 - -3.0 4,272,903 - +0.5 4,272,910 + +0.9 4,272,910 + -0.6 4,272,920 - -2.6 4,272,996 - -1.1 4,273,023 - -1.8 4,273,108 - -0.4 4,273,172 + -2.6 4,273,173 - -2.3 4,273,209 - +0.4 4,273,343 - BT3325 0.16 -4.5 4,273,359 + BT3325 0.18 -1.8 4,273,359 + BT3325 0.18 -2.1 4,273,469 - BT3325 0.27 +0.5 4,273,495 - BT3325 0.29 -3.7 4,273,503 - BT3325 0.29 +0.2 4,273,588 + BT3325 0.36 -1.8 4,273,589 - BT3325 0.36 -1.2 4,273,617 - BT3325 0.39 -1.5 4,273,623 - BT3325 0.39 -1.7 4,273,639 + BT3325 0.40 -3.8 4,273,640 - BT3325 0.40 -1.6 4,273,643 + BT3325 0.41 -0.8 4,273,658 + BT3325 0.42 -1.7 4,273,661 + BT3325 0.42 +0.2 4,273,662 - BT3325 0.42 +1.2 4,273,783 + BT3325 0.52 -3.1 4,273,810 - BT3325 0.54 -1.9 4,274,011 - BT3325 0.71 -1.4 4,274,048 - BT3325 0.74 -2.4 4,274,170 + BT3325 0.83 -3.6 4,274,171 - BT3325 0.84 -2.8 4,274,172 + BT3325 0.84 -1.7 4,274,173 - BT3325 0.84 -3.3 4,274,173 - BT3325 0.84 -3.4 4,274,252 - -0.3 4,274,252 - -1.5 4,274,294 - +0.2 4,274,296 + -0.4 4,274,358 - -2.4 4,274,460 + -2.5 4,274,461 - -1.4 4,274,461 - +0.2 4,274,518 + -0.2 4,274,562 + BT3326 0.11 -1.2 4,274,592 - BT3326 0.14 -0.8 4,274,615 + BT3326 0.17 +0.3 4,274,619 - BT3326 0.17 -1.1 4,274,619 - BT3326 0.17 -2.6 4,274,821 - BT3326 0.38 -0.3 4,275,165 - BT3326 0.75 -1.8 4,275,288 - BT3326 0.88 +0.2 4,275,300 + BT3326 0.89 -1.1 4,275,301 - BT3326 0.89 +3.5 4,275,304 - BT3326 0.89 +2.2
Or see this region's nucleotide sequence