Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT3231

Experiment: BHIS with Trimethoprim 0.00625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT3230 and tRNA-Gln are separated by 108 nucleotidestRNA-Gln and BT3231 are separated by 264 nucleotidesBT3231 and BT3232 are separated by 39 nucleotidesBT3232 and BT3233 are separated by 152 nucleotides BT3230: BT3230 - tyrosyl-tRNA synthetase (NCBI ptt file), at 4,119,353 to 4,120,645 BT3230 BT_t54: tRNA-Gln - tRNA-Gln (RefSeq), at 4,120,754 to 4,120,827 tRNA-Gln BT3231: BT3231 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (NCBI ptt file), at 4,121,092 to 4,121,934 BT3231 BT3232: BT3232 - gluconate 5-dehydrogenase (NCBI ptt file), at 4,121,974 to 4,122,777 BT3232 BT3233: BT3233 - predicted glycosaminoglycan binding protein (DUF4861) (from data), at 4,122,930 to 4,124,174 BT3233 Position (kb) 4121 4122Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 4120.842 kb on + strandat 4120.874 kb on + strandat 4120.885 kb on - strandat 4120.896 kb on + strandat 4120.897 kb on - strandat 4120.923 kb on + strandat 4120.926 kb on - strandat 4120.926 kb on - strandat 4121.037 kb on - strandat 4121.065 kb on + strandat 4121.065 kb on + strandat 4121.072 kb on + strandat 4121.082 kb on + strandat 4121.211 kb on + strand, within BT3231at 4121.219 kb on - strand, within BT3231at 4121.219 kb on - strand, within BT3231at 4121.339 kb on - strand, within BT3231at 4121.397 kb on + strand, within BT3231at 4121.506 kb on + strand, within BT3231at 4121.507 kb on - strand, within BT3231at 4121.540 kb on + strand, within BT3231at 4121.590 kb on - strand, within BT3231at 4121.609 kb on + strand, within BT3231at 4121.610 kb on - strand, within BT3231at 4121.616 kb on + strand, within BT3231at 4121.663 kb on + strand, within BT3231at 4121.710 kb on + strand, within BT3231at 4121.711 kb on - strand, within BT3231at 4121.743 kb on - strand, within BT3231at 4121.755 kb on - strand, within BT3231at 4121.783 kb on + strand, within BT3231at 4121.784 kb on - strand, within BT3231at 4121.920 kb on - strandat 4121.941 kb on - strandat 4121.945 kb on + strandat 4121.959 kb on + strandat 4122.170 kb on + strand, within BT3232at 4122.283 kb on - strand, within BT3232at 4122.415 kb on - strand, within BT3232at 4122.706 kb on - strandat 4122.706 kb on - strandat 4122.795 kb on + strandat 4122.796 kb on - strandat 4122.924 kb on + strandat 4122.927 kb on - strandat 4122.929 kb on + strandat 4122.929 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM
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4,120,842 + -0.6
4,120,874 + -1.5
4,120,885 - -1.5
4,120,896 + +2.4
4,120,897 - -1.8
4,120,923 + +3.3
4,120,926 - -2.6
4,120,926 - +1.1
4,121,037 - +0.4
4,121,065 + +0.1
4,121,065 + -1.7
4,121,072 + -0.5
4,121,082 + +1.6
4,121,211 + BT3231 0.14 -2.8
4,121,219 - BT3231 0.15 +1.1
4,121,219 - BT3231 0.15 -1.2
4,121,339 - BT3231 0.29 -0.7
4,121,397 + BT3231 0.36 -2.1
4,121,506 + BT3231 0.49 -2.3
4,121,507 - BT3231 0.49 +0.3
4,121,540 + BT3231 0.53 -1.5
4,121,590 - BT3231 0.59 -0.9
4,121,609 + BT3231 0.61 -1.9
4,121,610 - BT3231 0.61 -1.2
4,121,616 + BT3231 0.62 -0.3
4,121,663 + BT3231 0.68 -4.9
4,121,710 + BT3231 0.73 +0.1
4,121,711 - BT3231 0.73 -2.1
4,121,743 - BT3231 0.77 -2.5
4,121,755 - BT3231 0.79 -1.8
4,121,783 + BT3231 0.82 -2.0
4,121,784 - BT3231 0.82 -2.0
4,121,920 - -2.0
4,121,941 - -0.6
4,121,945 + +0.5
4,121,959 + +3.1
4,122,170 + BT3232 0.24 -3.5
4,122,283 - BT3232 0.38 +1.7
4,122,415 - BT3232 0.55 -1.3
4,122,706 - -1.5
4,122,706 - +0.7
4,122,795 + -1.7
4,122,796 - -2.2
4,122,924 + -0.5
4,122,927 - -2.4
4,122,929 + +0.1
4,122,929 + +2.1

Or see this region's nucleotide sequence