Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3230 and tRNA-Gln are separated by 108 nucleotides tRNA-Gln and BT3231 are separated by 264 nucleotides BT3231 and BT3232 are separated by 39 nucleotides BT3232 and BT3233 are separated by 152 nucleotides
BT3230: BT3230 - tyrosyl-tRNA synthetase (NCBI ptt file), at 4,119,353 to 4,120,645
BT3230
BT_t54: tRNA-Gln - tRNA-Gln (RefSeq), at 4,120,754 to 4,120,827
tRNA-Gln
BT3231: BT3231 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (NCBI ptt file), at 4,121,092 to 4,121,934
BT3231
BT3232: BT3232 - gluconate 5-dehydrogenase (NCBI ptt file), at 4,121,974 to 4,122,777
BT3232
BT3233: BT3233 - predicted glycosaminoglycan binding protein (DUF4861) (from data) , at 4,122,930 to 4,124,174
BT3233
Position (kb)
4121
4122 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4120.842 kb on + strand at 4120.874 kb on + strand at 4120.885 kb on - strand at 4120.896 kb on + strand at 4120.897 kb on - strand at 4120.923 kb on + strand at 4120.926 kb on - strand at 4120.926 kb on - strand at 4121.037 kb on - strand at 4121.065 kb on + strand at 4121.065 kb on + strand at 4121.072 kb on + strand at 4121.082 kb on + strand at 4121.211 kb on + strand, within BT3231 at 4121.219 kb on - strand, within BT3231 at 4121.219 kb on - strand, within BT3231 at 4121.339 kb on - strand, within BT3231 at 4121.397 kb on + strand, within BT3231 at 4121.506 kb on + strand, within BT3231 at 4121.507 kb on - strand, within BT3231 at 4121.540 kb on + strand, within BT3231 at 4121.590 kb on - strand, within BT3231 at 4121.609 kb on + strand, within BT3231 at 4121.610 kb on - strand, within BT3231 at 4121.616 kb on + strand, within BT3231 at 4121.663 kb on + strand, within BT3231 at 4121.710 kb on + strand, within BT3231 at 4121.711 kb on - strand, within BT3231 at 4121.743 kb on - strand, within BT3231 at 4121.755 kb on - strand, within BT3231 at 4121.783 kb on + strand, within BT3231 at 4121.784 kb on - strand, within BT3231 at 4121.920 kb on - strand at 4121.941 kb on - strand at 4121.945 kb on + strand at 4121.959 kb on + strand at 4122.170 kb on + strand, within BT3232 at 4122.283 kb on - strand, within BT3232 at 4122.415 kb on - strand, within BT3232 at 4122.706 kb on - strand at 4122.706 kb on - strand at 4122.795 kb on + strand at 4122.796 kb on - strand at 4122.924 kb on + strand at 4122.927 kb on - strand at 4122.929 kb on + strand at 4122.929 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 4,120,842 + -0.6 4,120,874 + -1.5 4,120,885 - -1.5 4,120,896 + +2.4 4,120,897 - -1.8 4,120,923 + +3.3 4,120,926 - -2.6 4,120,926 - +1.1 4,121,037 - +0.4 4,121,065 + +0.1 4,121,065 + -1.7 4,121,072 + -0.5 4,121,082 + +1.6 4,121,211 + BT3231 0.14 -2.8 4,121,219 - BT3231 0.15 +1.1 4,121,219 - BT3231 0.15 -1.2 4,121,339 - BT3231 0.29 -0.7 4,121,397 + BT3231 0.36 -2.1 4,121,506 + BT3231 0.49 -2.3 4,121,507 - BT3231 0.49 +0.3 4,121,540 + BT3231 0.53 -1.5 4,121,590 - BT3231 0.59 -0.9 4,121,609 + BT3231 0.61 -1.9 4,121,610 - BT3231 0.61 -1.2 4,121,616 + BT3231 0.62 -0.3 4,121,663 + BT3231 0.68 -4.9 4,121,710 + BT3231 0.73 +0.1 4,121,711 - BT3231 0.73 -2.1 4,121,743 - BT3231 0.77 -2.5 4,121,755 - BT3231 0.79 -1.8 4,121,783 + BT3231 0.82 -2.0 4,121,784 - BT3231 0.82 -2.0 4,121,920 - -2.0 4,121,941 - -0.6 4,121,945 + +0.5 4,121,959 + +3.1 4,122,170 + BT3232 0.24 -3.5 4,122,283 - BT3232 0.38 +1.7 4,122,415 - BT3232 0.55 -1.3 4,122,706 - -1.5 4,122,706 - +0.7 4,122,795 + -1.7 4,122,796 - -2.2 4,122,924 + -0.5 4,122,927 - -2.4 4,122,929 + +0.1 4,122,929 + +2.1
Or see this region's nucleotide sequence