Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3222 and BT3223 are separated by 87 nucleotides BT3223 and BT3224 are separated by 180 nucleotides BT3224 and BT3225 overlap by 4 nucleotides BT3225 and BT3226 are separated by 259 nucleotides
BT3222: BT3222 - hypothetical protein (NCBI ptt file), at 4,114,044 to 4,114,976
BT3222
BT3223: BT3223 - hypothetical protein (NCBI ptt file), at 4,115,064 to 4,115,645
BT3223
BT3224: BT3224 - putative lysine decarboxylase (NCBI ptt file), at 4,115,826 to 4,116,413
BT3224
BT3225: BT3225 - hypothetical protein (NCBI ptt file), at 4,116,410 to 4,117,132
BT3225
BT3226: BT3226 - hypothetical protein (NCBI ptt file), at 4,117,392 to 4,117,883
BT3226
Position (kb)
4115
4116
4117 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 4114.826 kb on + strand, within BT3222 at 4114.915 kb on + strand at 4114.974 kb on + strand at 4114.998 kb on + strand at 4114.998 kb on + strand at 4114.999 kb on - strand at 4115.040 kb on - strand at 4115.084 kb on - strand at 4115.087 kb on - strand at 4115.208 kb on + strand, within BT3223 at 4115.211 kb on - strand, within BT3223 at 4115.220 kb on + strand, within BT3223 at 4115.221 kb on - strand, within BT3223 at 4115.232 kb on + strand, within BT3223 at 4115.247 kb on + strand, within BT3223 at 4115.340 kb on + strand, within BT3223 at 4115.340 kb on + strand, within BT3223 at 4115.341 kb on - strand, within BT3223 at 4115.341 kb on - strand, within BT3223 at 4115.359 kb on - strand, within BT3223 at 4115.460 kb on - strand, within BT3223 at 4115.490 kb on - strand, within BT3223 at 4115.496 kb on - strand, within BT3223 at 4115.536 kb on + strand, within BT3223 at 4115.542 kb on + strand, within BT3223 at 4115.548 kb on - strand, within BT3223 at 4115.643 kb on + strand at 4115.668 kb on + strand at 4115.668 kb on + strand at 4115.669 kb on - strand at 4115.683 kb on + strand at 4115.683 kb on + strand at 4115.746 kb on + strand at 4115.771 kb on + strand at 4115.771 kb on + strand at 4115.836 kb on + strand at 4115.851 kb on + strand at 4115.875 kb on + strand at 4115.933 kb on + strand, within BT3224 at 4115.933 kb on + strand, within BT3224 at 4116.238 kb on + strand, within BT3224 at 4116.238 kb on + strand, within BT3224 at 4116.283 kb on + strand, within BT3224 at 4116.317 kb on + strand, within BT3224 at 4116.317 kb on + strand, within BT3224 at 4116.319 kb on + strand, within BT3224
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 4,114,826 + BT3222 0.84 +0.0 4,114,915 + +0.8 4,114,974 + +3.5 4,114,998 + +0.3 4,114,998 + +1.3 4,114,999 - -2.7 4,115,040 - -2.2 4,115,084 - -3.6 4,115,087 - -2.5 4,115,208 + BT3223 0.25 -2.5 4,115,211 - BT3223 0.25 -3.4 4,115,220 + BT3223 0.27 -4.6 4,115,221 - BT3223 0.27 -2.9 4,115,232 + BT3223 0.29 -1.9 4,115,247 + BT3223 0.31 -0.9 4,115,340 + BT3223 0.47 -3.7 4,115,340 + BT3223 0.47 +0.5 4,115,341 - BT3223 0.48 -2.9 4,115,341 - BT3223 0.48 -0.9 4,115,359 - BT3223 0.51 +1.1 4,115,460 - BT3223 0.68 -0.9 4,115,490 - BT3223 0.73 -1.5 4,115,496 - BT3223 0.74 +0.1 4,115,536 + BT3223 0.81 -0.9 4,115,542 + BT3223 0.82 +1.1 4,115,548 - BT3223 0.83 -1.7 4,115,643 + -0.9 4,115,668 + -0.9 4,115,668 + -2.6 4,115,669 - +0.8 4,115,683 + +4.4 4,115,683 + -0.6 4,115,746 + -3.2 4,115,771 + -1.3 4,115,771 + -2.0 4,115,836 + -3.4 4,115,851 + +0.1 4,115,875 + +0.7 4,115,933 + BT3224 0.18 -4.3 4,115,933 + BT3224 0.18 -2.5 4,116,238 + BT3224 0.70 -2.1 4,116,238 + BT3224 0.70 -5.3 4,116,283 + BT3224 0.78 -0.8 4,116,317 + BT3224 0.84 -3.9 4,116,317 + BT3224 0.84 -0.5 4,116,319 + BT3224 0.84 -2.7
Or see this region's nucleotide sequence