Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2461 and BT2462 are separated by 160 nucleotides BT2462 and BT2463 are separated by 102 nucleotides BT2463 and BT2464 are separated by 137 nucleotides
BT2461: BT2461 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 3,080,348 to 3,083,752
BT2461
BT2462: BT2462 - putative anti-sigma factor (NCBI ptt file), at 3,083,913 to 3,084,848
BT2462
BT2463: BT2463 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 3,084,951 to 3,085,520
BT2463
BT2464: BT2464 - hypothetical protein (NCBI ptt file), at 3,085,658 to 3,085,924
BT2464
Position (kb)
3083
3084
3085 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3082.922 kb on - strand, within BT2461 at 3082.922 kb on - strand, within BT2461 at 3082.937 kb on + strand, within BT2461 at 3082.937 kb on + strand, within BT2461 at 3082.937 kb on + strand, within BT2461 at 3082.937 kb on + strand, within BT2461 at 3082.955 kb on - strand, within BT2461 at 3082.955 kb on - strand, within BT2461 at 3083.032 kb on + strand, within BT2461 at 3083.101 kb on + strand, within BT2461 at 3083.210 kb on - strand, within BT2461 at 3083.210 kb on - strand, within BT2461 at 3083.212 kb on - strand, within BT2461 at 3083.212 kb on - strand, within BT2461 at 3083.212 kb on - strand, within BT2461 at 3083.212 kb on - strand, within BT2461 at 3083.227 kb on + strand, within BT2461 at 3083.272 kb on + strand, within BT2461 at 3083.305 kb on + strand, within BT2461 at 3083.321 kb on - strand, within BT2461 at 3083.342 kb on + strand, within BT2461 at 3083.396 kb on - strand, within BT2461 at 3083.426 kb on + strand at 3083.426 kb on + strand at 3083.427 kb on - strand at 3083.427 kb on - strand at 3083.427 kb on - strand at 3083.429 kb on - strand at 3083.429 kb on - strand at 3083.456 kb on + strand at 3083.458 kb on + strand at 3083.485 kb on + strand at 3083.485 kb on + strand at 3083.509 kb on + strand at 3083.519 kb on - strand at 3083.524 kb on + strand at 3083.525 kb on - strand at 3083.551 kb on - strand at 3083.594 kb on + strand at 3083.594 kb on + strand at 3083.595 kb on - strand at 3083.643 kb on - strand at 3083.645 kb on - strand at 3083.680 kb on - strand at 3083.699 kb on - strand at 3083.736 kb on + strand at 3083.768 kb on + strand at 3083.768 kb on + strand at 3083.782 kb on + strand at 3083.792 kb on + strand at 3083.793 kb on - strand at 3083.798 kb on + strand at 3083.813 kb on + strand at 3083.897 kb on + strand at 3083.930 kb on + strand at 3083.985 kb on + strand at 3083.988 kb on + strand at 3084.057 kb on - strand, within BT2462 at 3084.076 kb on + strand, within BT2462 at 3084.077 kb on - strand, within BT2462 at 3084.077 kb on - strand, within BT2462 at 3084.077 kb on - strand, within BT2462 at 3084.087 kb on + strand, within BT2462 at 3084.131 kb on + strand, within BT2462 at 3084.132 kb on - strand, within BT2462 at 3084.155 kb on - strand, within BT2462 at 3084.164 kb on + strand, within BT2462 at 3084.164 kb on + strand, within BT2462 at 3084.199 kb on - strand, within BT2462 at 3084.237 kb on + strand, within BT2462 at 3084.294 kb on + strand, within BT2462 at 3084.294 kb on + strand, within BT2462 at 3084.296 kb on + strand, within BT2462 at 3084.296 kb on + strand, within BT2462 at 3084.297 kb on - strand, within BT2462 at 3084.297 kb on - strand, within BT2462 at 3084.300 kb on - strand, within BT2462 at 3084.300 kb on - strand, within BT2462 at 3084.389 kb on + strand, within BT2462 at 3084.426 kb on + strand, within BT2462 at 3084.489 kb on + strand, within BT2462 at 3084.489 kb on + strand, within BT2462 at 3084.513 kb on + strand, within BT2462 at 3084.513 kb on + strand, within BT2462 at 3084.513 kb on + strand, within BT2462 at 3084.514 kb on - strand, within BT2462 at 3084.612 kb on - strand, within BT2462 at 3084.637 kb on - strand, within BT2462 at 3084.637 kb on - strand, within BT2462 at 3084.642 kb on - strand, within BT2462 at 3084.684 kb on + strand, within BT2462 at 3084.702 kb on - strand, within BT2462 at 3084.724 kb on + strand, within BT2462 at 3084.731 kb on + strand, within BT2462 at 3084.745 kb on + strand, within BT2462 at 3084.838 kb on - strand at 3084.869 kb on + strand at 3084.870 kb on - strand at 3084.872 kb on - strand at 3084.952 kb on + strand at 3084.953 kb on - strand at 3084.973 kb on + strand at 3085.016 kb on + strand, within BT2463 at 3085.016 kb on + strand, within BT2463 at 3085.017 kb on - strand, within BT2463 at 3085.029 kb on + strand, within BT2463 at 3085.030 kb on - strand, within BT2463 at 3085.031 kb on + strand, within BT2463 at 3085.032 kb on - strand, within BT2463 at 3085.112 kb on + strand, within BT2463 at 3085.113 kb on - strand, within BT2463 at 3085.117 kb on - strand, within BT2463 at 3085.137 kb on + strand, within BT2463 at 3085.138 kb on - strand, within BT2463 at 3085.138 kb on - strand, within BT2463 at 3085.185 kb on + strand, within BT2463 at 3085.186 kb on - strand, within BT2463 at 3085.199 kb on + strand, within BT2463 at 3085.200 kb on - strand, within BT2463 at 3085.230 kb on + strand, within BT2463 at 3085.230 kb on + strand, within BT2463 at 3085.294 kb on - strand, within BT2463 at 3085.294 kb on - strand, within BT2463 at 3085.298 kb on - strand, within BT2463 at 3085.311 kb on - strand, within BT2463 at 3085.403 kb on + strand, within BT2463 at 3085.403 kb on + strand, within BT2463 at 3085.430 kb on + strand, within BT2463 at 3085.433 kb on - strand, within BT2463 at 3085.433 kb on - strand, within BT2463 at 3085.433 kb on - strand, within BT2463 at 3085.474 kb on + strand at 3085.475 kb on - strand at 3085.475 kb on - strand at 3085.475 kb on - strand at 3085.485 kb on - strand at 3085.534 kb on - strand at 3085.563 kb on + strand at 3085.573 kb on + strand at 3085.602 kb on - strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.623 kb on + strand at 3085.749 kb on - strand, within BT2464 at 3085.758 kb on - strand, within BT2464 at 3085.763 kb on - strand, within BT2464 at 3085.765 kb on + strand, within BT2464 at 3085.790 kb on - strand, within BT2464 at 3085.793 kb on + strand, within BT2464 at 3085.793 kb on + strand, within BT2464 at 3085.793 kb on + strand, within BT2464 at 3085.794 kb on - strand, within BT2464 at 3085.794 kb on - strand, within BT2464
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 3,082,922 - BT2461 0.76 +2.6 3,082,922 - BT2461 0.76 +0.0 3,082,937 + BT2461 0.76 -1.1 3,082,937 + BT2461 0.76 +4.4 3,082,937 + BT2461 0.76 -2.5 3,082,937 + BT2461 0.76 +0.8 3,082,955 - BT2461 0.77 +2.6 3,082,955 - BT2461 0.77 -2.1 3,083,032 + BT2461 0.79 +2.9 3,083,101 + BT2461 0.81 +0.0 3,083,210 - BT2461 0.84 -2.1 3,083,210 - BT2461 0.84 -1.4 3,083,212 - BT2461 0.84 +3.3 3,083,212 - BT2461 0.84 +0.0 3,083,212 - BT2461 0.84 -1.7 3,083,212 - BT2461 0.84 +0.2 3,083,227 + BT2461 0.85 +1.3 3,083,272 + BT2461 0.86 -2.4 3,083,305 + BT2461 0.87 -2.5 3,083,321 - BT2461 0.87 +0.8 3,083,342 + BT2461 0.88 +0.6 3,083,396 - BT2461 0.90 -5.0 3,083,426 + -0.2 3,083,426 + -2.5 3,083,427 - -2.5 3,083,427 - -0.9 3,083,427 - -5.0 3,083,429 - +2.2 3,083,429 - +3.4 3,083,456 + -1.5 3,083,458 + +0.6 3,083,485 + -0.5 3,083,485 + -3.0 3,083,509 + +1.0 3,083,519 - +1.0 3,083,524 + -1.5 3,083,525 - +2.9 3,083,551 - +0.7 3,083,594 + -0.5 3,083,594 + -4.3 3,083,595 - -3.7 3,083,643 - +0.0 3,083,645 - -1.7 3,083,680 - -2.0 3,083,699 - +3.0 3,083,736 + -2.3 3,083,768 + -2.0 3,083,768 + -1.0 3,083,782 + -1.2 3,083,792 + +1.0 3,083,793 - -1.0 3,083,798 + +0.5 3,083,813 + -1.8 3,083,897 + -1.0 3,083,930 + -0.3 3,083,985 + -0.5 3,083,988 + +1.0 3,084,057 - BT2462 0.15 +2.6 3,084,076 + BT2462 0.17 -3.4 3,084,077 - BT2462 0.18 -2.5 3,084,077 - BT2462 0.18 +0.2 3,084,077 - BT2462 0.18 -2.8 3,084,087 + BT2462 0.19 +0.6 3,084,131 + BT2462 0.23 -1.7 3,084,132 - BT2462 0.23 -2.3 3,084,155 - BT2462 0.26 +2.9 3,084,164 + BT2462 0.27 -2.5 3,084,164 + BT2462 0.27 -0.9 3,084,199 - BT2462 0.31 -1.3 3,084,237 + BT2462 0.35 +1.9 3,084,294 + BT2462 0.41 -3.3 3,084,294 + BT2462 0.41 -2.5 3,084,296 + BT2462 0.41 +0.0 3,084,296 + BT2462 0.41 -1.6 3,084,297 - BT2462 0.41 -0.2 3,084,297 - BT2462 0.41 -3.1 3,084,300 - BT2462 0.41 -0.4 3,084,300 - BT2462 0.41 -1.4 3,084,389 + BT2462 0.51 +0.0 3,084,426 + BT2462 0.55 -1.4 3,084,489 + BT2462 0.62 -1.0 3,084,489 + BT2462 0.62 -4.3 3,084,513 + BT2462 0.64 -0.8 3,084,513 + BT2462 0.64 -2.8 3,084,513 + BT2462 0.64 +0.3 3,084,514 - BT2462 0.64 -3.0 3,084,612 - BT2462 0.75 +1.0 3,084,637 - BT2462 0.77 +1.6 3,084,637 - BT2462 0.77 +0.5 3,084,642 - BT2462 0.78 -0.5 3,084,684 + BT2462 0.82 -1.8 3,084,702 - BT2462 0.84 +2.9 3,084,724 + BT2462 0.87 -1.0 3,084,731 + BT2462 0.87 -4.0 3,084,745 + BT2462 0.89 -2.0 3,084,838 - -2.1 3,084,869 + +2.0 3,084,870 - -2.0 3,084,872 - +1.0 3,084,952 + -0.9 3,084,953 - -1.0 3,084,973 + -1.0 3,085,016 + BT2463 0.11 -2.2 3,085,016 + BT2463 0.11 -1.5 3,085,017 - BT2463 0.12 +1.0 3,085,029 + BT2463 0.14 -3.5 3,085,030 - BT2463 0.14 +0.7 3,085,031 + BT2463 0.14 +0.9 3,085,032 - BT2463 0.14 +1.0 3,085,112 + BT2463 0.28 -4.1 3,085,113 - BT2463 0.28 -0.4 3,085,117 - BT2463 0.29 +1.5 3,085,137 + BT2463 0.33 -1.3 3,085,138 - BT2463 0.33 +1.6 3,085,138 - BT2463 0.33 -0.4 3,085,185 + BT2463 0.41 +1.3 3,085,186 - BT2463 0.41 -3.0 3,085,199 + BT2463 0.44 +2.6 3,085,200 - BT2463 0.44 +0.6 3,085,230 + BT2463 0.49 +1.4 3,085,230 + BT2463 0.49 +0.5 3,085,294 - BT2463 0.60 +2.7 3,085,294 - BT2463 0.60 -1.3 3,085,298 - BT2463 0.61 +1.6 3,085,311 - BT2463 0.63 +2.9 3,085,403 + BT2463 0.79 -2.3 3,085,403 + BT2463 0.79 -0.8 3,085,430 + BT2463 0.84 +2.2 3,085,433 - BT2463 0.85 -0.9 3,085,433 - BT2463 0.85 +2.1 3,085,433 - BT2463 0.85 -2.8 3,085,474 + -1.0 3,085,475 - -3.0 3,085,475 - -2.3 3,085,475 - +0.5 3,085,485 - -2.1 3,085,534 - +1.3 3,085,563 + -2.0 3,085,573 + +0.0 3,085,602 - -2.5 3,085,617 + -1.4 3,085,617 + -0.5 3,085,623 + +1.6 3,085,749 - BT2464 0.34 +1.6 3,085,758 - BT2464 0.37 +0.3 3,085,763 - BT2464 0.39 +2.4 3,085,765 + BT2464 0.40 -4.5 3,085,790 - BT2464 0.49 -1.0 3,085,793 + BT2464 0.51 -0.4 3,085,793 + BT2464 0.51 -1.4 3,085,793 + BT2464 0.51 -0.5 3,085,794 - BT2464 0.51 -1.5 3,085,794 - BT2464 0.51 -2.0
Or see this region's nucleotide sequence