Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2439 and BT2440 overlap by 4 nucleotides BT2440 and BT2441 are separated by 35 nucleotides BT2441 and BT2442 are separated by 441 nucleotides
BT2439: BT2439 - beta-N-acetylglucosaminidase (NCBI ptt file), at 3,053,404 to 3,056,409
BT2439
BT2440: BT2440 - 5'-nucleotidase precursor (NCBI ptt file), at 3,056,406 to 3,057,293
BT2440
BT2441: BT2441 - putative 5'-nucleotidase/2',3'-cyclic phosphodiesterase (NCBI ptt file), at 3,057,329 to 3,058,165
BT2441
BT2442: BT2442 - major outer membrane protein OmpA (NCBI ptt file), at 3,058,607 to 3,059,701
BT2442
Position (kb)
3057
3058
3059 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3056.337 kb on + strand at 3056.337 kb on + strand at 3056.337 kb on + strand at 3056.374 kb on + strand at 3056.389 kb on + strand at 3056.390 kb on - strand at 3056.390 kb on - strand at 3056.391 kb on + strand at 3056.391 kb on + strand at 3056.392 kb on - strand at 3056.392 kb on - strand at 3056.393 kb on + strand at 3056.393 kb on + strand at 3056.393 kb on + strand at 3056.394 kb on - strand at 3056.398 kb on + strand at 3056.401 kb on + strand at 3056.402 kb on - strand at 3056.430 kb on + strand at 3056.547 kb on - strand, within BT2440 at 3056.559 kb on - strand, within BT2440 at 3056.572 kb on - strand, within BT2440 at 3056.613 kb on + strand, within BT2440 at 3056.613 kb on + strand, within BT2440 at 3056.613 kb on + strand, within BT2440 at 3056.613 kb on + strand, within BT2440 at 3056.614 kb on - strand, within BT2440 at 3056.614 kb on - strand, within BT2440 at 3056.683 kb on + strand, within BT2440 at 3056.683 kb on + strand, within BT2440 at 3056.782 kb on + strand, within BT2440 at 3056.824 kb on + strand, within BT2440 at 3056.835 kb on + strand, within BT2440 at 3056.835 kb on + strand, within BT2440 at 3056.835 kb on + strand, within BT2440 at 3056.835 kb on + strand, within BT2440 at 3056.836 kb on - strand, within BT2440 at 3056.836 kb on - strand, within BT2440 at 3056.924 kb on - strand, within BT2440 at 3056.952 kb on + strand, within BT2440 at 3056.963 kb on + strand, within BT2440 at 3056.972 kb on + strand, within BT2440 at 3056.972 kb on + strand, within BT2440 at 3056.973 kb on - strand, within BT2440 at 3056.977 kb on + strand, within BT2440 at 3056.997 kb on - strand, within BT2440 at 3057.129 kb on + strand, within BT2440 at 3057.130 kb on - strand, within BT2440 at 3057.189 kb on + strand, within BT2440 at 3057.192 kb on + strand, within BT2440 at 3057.237 kb on + strand at 3057.238 kb on - strand at 3057.257 kb on + strand at 3057.264 kb on - strand at 3057.264 kb on - strand at 3057.264 kb on - strand at 3057.276 kb on - strand at 3057.281 kb on - strand at 3057.307 kb on + strand at 3057.307 kb on + strand at 3057.308 kb on - strand at 3057.312 kb on - strand at 3057.362 kb on - strand at 3057.362 kb on - strand at 3057.414 kb on - strand, within BT2441 at 3057.524 kb on - strand, within BT2441 at 3057.534 kb on + strand, within BT2441 at 3057.566 kb on + strand, within BT2441 at 3057.645 kb on - strand, within BT2441 at 3057.742 kb on + strand, within BT2441 at 3057.743 kb on - strand, within BT2441 at 3057.745 kb on + strand, within BT2441 at 3057.745 kb on + strand, within BT2441 at 3057.759 kb on - strand, within BT2441 at 3057.778 kb on + strand, within BT2441 at 3057.915 kb on + strand, within BT2441 at 3057.925 kb on + strand, within BT2441 at 3057.926 kb on - strand, within BT2441 at 3057.943 kb on - strand, within BT2441 at 3058.057 kb on + strand, within BT2441 at 3058.083 kb on - strand at 3058.096 kb on + strand at 3058.115 kb on + strand at 3058.148 kb on + strand at 3058.149 kb on - strand at 3058.235 kb on - strand at 3058.235 kb on - strand at 3058.420 kb on + strand at 3058.420 kb on + strand at 3058.452 kb on + strand at 3058.476 kb on + strand at 3058.510 kb on - strand at 3058.518 kb on + strand at 3058.519 kb on - strand at 3058.582 kb on + strand at 3058.588 kb on - strand at 3058.588 kb on - strand at 3058.625 kb on - strand at 3058.687 kb on + strand at 3058.688 kb on - strand at 3058.688 kb on - strand at 3058.771 kb on + strand, within BT2442 at 3058.788 kb on - strand, within BT2442 at 3058.788 kb on - strand, within BT2442 at 3058.797 kb on + strand, within BT2442 at 3058.840 kb on - strand, within BT2442 at 3058.845 kb on - strand, within BT2442 at 3058.852 kb on + strand, within BT2442 at 3058.852 kb on + strand, within BT2442 at 3058.852 kb on + strand, within BT2442 at 3058.852 kb on + strand, within BT2442 at 3058.852 kb on + strand, within BT2442 at 3058.853 kb on - strand, within BT2442 at 3058.932 kb on - strand, within BT2442 at 3059.013 kb on + strand, within BT2442 at 3059.013 kb on + strand, within BT2442 at 3059.016 kb on - strand, within BT2442
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 3,056,337 + -2.0 3,056,337 + -2.2 3,056,337 + -2.2 3,056,374 + +2.9 3,056,389 + -0.7 3,056,390 - -2.1 3,056,390 - +2.6 3,056,391 + -0.2 3,056,391 + -1.5 3,056,392 - -1.4 3,056,392 - -0.4 3,056,393 + -1.3 3,056,393 + -0.5 3,056,393 + -2.8 3,056,394 - -3.9 3,056,398 + +0.4 3,056,401 + -3.9 3,056,402 - +0.1 3,056,430 + +2.4 3,056,547 - BT2440 0.16 -2.3 3,056,559 - BT2440 0.17 -2.4 3,056,572 - BT2440 0.19 -1.1 3,056,613 + BT2440 0.23 -1.4 3,056,613 + BT2440 0.23 -5.0 3,056,613 + BT2440 0.23 -1.7 3,056,613 + BT2440 0.23 -2.1 3,056,614 - BT2440 0.23 +0.7 3,056,614 - BT2440 0.23 -3.1 3,056,683 + BT2440 0.31 -1.9 3,056,683 + BT2440 0.31 +3.2 3,056,782 + BT2440 0.42 +0.1 3,056,824 + BT2440 0.47 -0.9 3,056,835 + BT2440 0.48 -4.6 3,056,835 + BT2440 0.48 -2.5 3,056,835 + BT2440 0.48 +0.6 3,056,835 + BT2440 0.48 -0.2 3,056,836 - BT2440 0.48 -0.9 3,056,836 - BT2440 0.48 +3.5 3,056,924 - BT2440 0.58 -0.7 3,056,952 + BT2440 0.61 -1.7 3,056,963 + BT2440 0.63 +0.8 3,056,972 + BT2440 0.64 -0.9 3,056,972 + BT2440 0.64 -2.1 3,056,973 - BT2440 0.64 -2.5 3,056,977 + BT2440 0.64 -1.5 3,056,997 - BT2440 0.67 -3.0 3,057,129 + BT2440 0.81 +0.9 3,057,130 - BT2440 0.82 -2.5 3,057,189 + BT2440 0.88 -2.2 3,057,192 + BT2440 0.89 +1.6 3,057,237 + +0.8 3,057,238 - -2.7 3,057,257 + -0.9 3,057,264 - +0.1 3,057,264 - +0.1 3,057,264 - +1.1 3,057,276 - -1.1 3,057,281 - -1.5 3,057,307 + -2.7 3,057,307 + -1.5 3,057,308 - -1.9 3,057,312 - +0.8 3,057,362 - +2.5 3,057,362 - -0.5 3,057,414 - BT2441 0.10 +1.6 3,057,524 - BT2441 0.23 +1.1 3,057,534 + BT2441 0.24 -1.7 3,057,566 + BT2441 0.28 +1.1 3,057,645 - BT2441 0.38 -3.4 3,057,742 + BT2441 0.49 +2.4 3,057,743 - BT2441 0.49 +0.1 3,057,745 + BT2441 0.50 -1.9 3,057,745 + BT2441 0.50 -0.9 3,057,759 - BT2441 0.51 -0.7 3,057,778 + BT2441 0.54 -3.6 3,057,915 + BT2441 0.70 -2.2 3,057,925 + BT2441 0.71 -3.8 3,057,926 - BT2441 0.71 -2.5 3,057,943 - BT2441 0.73 -1.5 3,058,057 + BT2441 0.87 -2.0 3,058,083 - +0.9 3,058,096 + -2.8 3,058,115 + -3.7 3,058,148 + -2.5 3,058,149 - +2.4 3,058,235 - +3.5 3,058,235 - +2.6 3,058,420 + +2.8 3,058,420 + -1.5 3,058,452 + +0.1 3,058,476 + -0.9 3,058,510 - +0.6 3,058,518 + -1.5 3,058,519 - +0.1 3,058,582 + -2.5 3,058,588 - -0.8 3,058,588 - +0.1 3,058,625 - -1.8 3,058,687 + -1.8 3,058,688 - +0.1 3,058,688 - +0.1 3,058,771 + BT2442 0.15 -1.5 3,058,788 - BT2442 0.17 -1.4 3,058,788 - BT2442 0.17 -1.6 3,058,797 + BT2442 0.17 -0.7 3,058,840 - BT2442 0.21 -3.2 3,058,845 - BT2442 0.22 -1.8 3,058,852 + BT2442 0.22 +1.1 3,058,852 + BT2442 0.22 +0.9 3,058,852 + BT2442 0.22 -1.1 3,058,852 + BT2442 0.22 -3.9 3,058,852 + BT2442 0.22 +0.1 3,058,853 - BT2442 0.22 -1.6 3,058,932 - BT2442 0.30 +0.3 3,059,013 + BT2442 0.37 +2.1 3,059,013 + BT2442 0.37 -3.4 3,059,016 - BT2442 0.37 -0.3
Or see this region's nucleotide sequence