Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2342 and BT2343 are separated by 52 nucleotides BT2343 and BT2344 are separated by 152 nucleotides BT2344 and BT2345 are separated by 14 nucleotides BT2345 and BT2346 are separated by 19 nucleotides
BT2342: BT2342 - transcription regulator (NCBI ptt file), at 2,922,778 to 2,923,575
BT2342
BT2343: BT2343 - hypothetical protein (NCBI ptt file), at 2,923,628 to 2,924,014
BT2343
BT2344: BT2344 - hypothetical protein (NCBI ptt file), at 2,924,167 to 2,924,409
BT2344
BT2345: BT2345 - hypothetical protein (NCBI ptt file), at 2,924,424 to 2,925,062
BT2345
BT2346: BT2346 - iron-sulfur cluster-binding protein (NCBI ptt file), at 2,925,082 to 2,925,744
BT2346
Position (kb)
2924
2925 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2923.196 kb on + strand, within BT2342 at 2923.251 kb on - strand, within BT2342 at 2923.258 kb on - strand, within BT2342 at 2923.350 kb on - strand, within BT2342 at 2923.410 kb on + strand, within BT2342 at 2923.411 kb on - strand, within BT2342 at 2923.411 kb on - strand, within BT2342 at 2923.411 kb on - strand, within BT2342 at 2923.411 kb on - strand, within BT2342 at 2923.447 kb on - strand, within BT2342 at 2923.491 kb on - strand, within BT2342 at 2923.510 kb on - strand at 2923.572 kb on - strand at 2923.572 kb on - strand at 2923.572 kb on - strand at 2923.662 kb on - strand at 2923.682 kb on - strand, within BT2343 at 2923.684 kb on - strand, within BT2343 at 2923.684 kb on - strand, within BT2343 at 2923.704 kb on + strand, within BT2343 at 2923.704 kb on + strand, within BT2343 at 2923.705 kb on - strand, within BT2343 at 2923.705 kb on - strand, within BT2343 at 2923.706 kb on + strand, within BT2343 at 2923.707 kb on - strand, within BT2343 at 2923.713 kb on + strand, within BT2343 at 2923.713 kb on + strand, within BT2343 at 2923.747 kb on - strand, within BT2343 at 2923.759 kb on - strand, within BT2343 at 2923.812 kb on + strand, within BT2343 at 2923.823 kb on - strand, within BT2343 at 2923.828 kb on + strand, within BT2343 at 2923.853 kb on + strand, within BT2343 at 2923.854 kb on - strand, within BT2343 at 2923.854 kb on - strand, within BT2343 at 2923.859 kb on - strand, within BT2343 at 2923.898 kb on + strand, within BT2343 at 2923.904 kb on - strand, within BT2343 at 2923.935 kb on + strand, within BT2343 at 2923.936 kb on - strand, within BT2343 at 2923.936 kb on - strand, within BT2343 at 2923.982 kb on - strand at 2923.982 kb on - strand at 2923.983 kb on + strand at 2924.010 kb on + strand at 2924.010 kb on + strand at 2924.012 kb on + strand at 2924.013 kb on - strand at 2924.013 kb on - strand at 2924.013 kb on - strand at 2924.016 kb on - strand at 2924.025 kb on + strand at 2924.031 kb on + strand at 2924.037 kb on - strand at 2924.043 kb on - strand at 2924.059 kb on + strand at 2924.073 kb on + strand at 2924.080 kb on + strand at 2924.080 kb on + strand at 2924.146 kb on - strand at 2924.146 kb on - strand at 2924.148 kb on + strand at 2924.149 kb on - strand at 2924.150 kb on + strand at 2924.151 kb on - strand at 2924.164 kb on + strand at 2924.245 kb on + strand, within BT2344 at 2924.245 kb on + strand, within BT2344 at 2924.246 kb on - strand, within BT2344 at 2924.289 kb on + strand, within BT2344 at 2924.348 kb on - strand, within BT2344 at 2924.391 kb on + strand at 2924.392 kb on - strand at 2924.435 kb on - strand at 2924.435 kb on - strand at 2924.522 kb on + strand, within BT2345 at 2924.523 kb on - strand, within BT2345 at 2924.606 kb on + strand, within BT2345 at 2924.606 kb on + strand, within BT2345 at 2924.607 kb on - strand, within BT2345 at 2924.607 kb on - strand, within BT2345 at 2924.607 kb on - strand, within BT2345 at 2924.676 kb on - strand, within BT2345 at 2924.676 kb on - strand, within BT2345 at 2924.757 kb on + strand, within BT2345 at 2924.878 kb on + strand, within BT2345 at 2924.878 kb on + strand, within BT2345 at 2924.878 kb on + strand, within BT2345 at 2924.878 kb on + strand, within BT2345 at 2924.878 kb on + strand, within BT2345 at 2924.879 kb on - strand, within BT2345 at 2924.908 kb on + strand, within BT2345 at 2924.934 kb on - strand, within BT2345 at 2924.934 kb on - strand, within BT2345 at 2924.939 kb on - strand, within BT2345 at 2924.953 kb on - strand, within BT2345 at 2924.953 kb on - strand, within BT2345 at 2924.953 kb on - strand, within BT2345 at 2924.956 kb on + strand, within BT2345 at 2924.957 kb on - strand, within BT2345 at 2924.957 kb on - strand, within BT2345 at 2924.964 kb on + strand, within BT2345 at 2924.964 kb on + strand, within BT2345 at 2924.965 kb on - strand, within BT2345 at 2924.965 kb on - strand, within BT2345 at 2925.001 kb on + strand at 2925.002 kb on - strand at 2925.007 kb on + strand at 2925.030 kb on - strand at 2925.063 kb on + strand at 2925.068 kb on + strand at 2925.068 kb on + strand at 2925.108 kb on - strand at 2925.162 kb on - strand, within BT2346 at 2925.162 kb on - strand, within BT2346 at 2925.195 kb on + strand, within BT2346 at 2925.207 kb on + strand, within BT2346 at 2925.246 kb on + strand, within BT2346 at 2925.262 kb on - strand, within BT2346 at 2925.264 kb on - strand, within BT2346 at 2925.264 kb on - strand, within BT2346 at 2925.274 kb on - strand, within BT2346 at 2925.297 kb on + strand, within BT2346 at 2925.297 kb on + strand, within BT2346 at 2925.353 kb on - strand, within BT2346
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 2,923,196 + BT2342 0.52 -1.4 2,923,251 - BT2342 0.59 -3.1 2,923,258 - BT2342 0.60 +1.6 2,923,350 - BT2342 0.72 -0.9 2,923,410 + BT2342 0.79 -1.5 2,923,411 - BT2342 0.79 +2.4 2,923,411 - BT2342 0.79 -0.4 2,923,411 - BT2342 0.79 +1.2 2,923,411 - BT2342 0.79 +1.8 2,923,447 - BT2342 0.84 +3.0 2,923,491 - BT2342 0.89 -2.7 2,923,510 - -3.0 2,923,572 - -2.5 2,923,572 - -0.7 2,923,572 - -1.0 2,923,662 - -2.5 2,923,682 - BT2343 0.14 -0.8 2,923,684 - BT2343 0.14 +3.2 2,923,684 - BT2343 0.14 -1.5 2,923,704 + BT2343 0.20 +1.9 2,923,704 + BT2343 0.20 -2.3 2,923,705 - BT2343 0.20 -0.1 2,923,705 - BT2343 0.20 +0.8 2,923,706 + BT2343 0.20 -2.3 2,923,707 - BT2343 0.20 -2.8 2,923,713 + BT2343 0.22 +0.4 2,923,713 + BT2343 0.22 -1.5 2,923,747 - BT2343 0.31 +1.9 2,923,759 - BT2343 0.34 -2.0 2,923,812 + BT2343 0.48 +2.6 2,923,823 - BT2343 0.50 -1.2 2,923,828 + BT2343 0.52 -1.5 2,923,853 + BT2343 0.58 -0.1 2,923,854 - BT2343 0.58 +0.3 2,923,854 - BT2343 0.58 -2.5 2,923,859 - BT2343 0.60 +0.0 2,923,898 + BT2343 0.70 -0.5 2,923,904 - BT2343 0.71 -1.0 2,923,935 + BT2343 0.79 +0.3 2,923,936 - BT2343 0.80 -2.3 2,923,936 - BT2343 0.80 +0.0 2,923,982 - -1.9 2,923,982 - +3.4 2,923,983 + -3.7 2,924,010 + -1.0 2,924,010 + -2.0 2,924,012 + -1.5 2,924,013 - +3.0 2,924,013 - -2.5 2,924,013 - -1.2 2,924,016 - -1.8 2,924,025 + -2.8 2,924,031 + -3.7 2,924,037 - -1.0 2,924,043 - +0.0 2,924,059 + -0.2 2,924,073 + +1.0 2,924,080 + +0.5 2,924,080 + -3.4 2,924,146 - +2.2 2,924,146 - -3.8 2,924,148 + -1.3 2,924,149 - -1.3 2,924,150 + -2.5 2,924,151 - -2.9 2,924,164 + -2.2 2,924,245 + BT2344 0.32 +2.6 2,924,245 + BT2344 0.32 -2.0 2,924,246 - BT2344 0.33 +0.0 2,924,289 + BT2344 0.50 +4.2 2,924,348 - BT2344 0.74 +2.5 2,924,391 + -1.0 2,924,392 - -1.5 2,924,435 - -2.4 2,924,435 - +0.9 2,924,522 + BT2345 0.15 +0.6 2,924,523 - BT2345 0.15 -0.4 2,924,606 + BT2345 0.28 -0.5 2,924,606 + BT2345 0.28 +0.0 2,924,607 - BT2345 0.29 -1.7 2,924,607 - BT2345 0.29 -0.8 2,924,607 - BT2345 0.29 -1.8 2,924,676 - BT2345 0.39 -0.7 2,924,676 - BT2345 0.39 +3.0 2,924,757 + BT2345 0.52 +3.0 2,924,878 + BT2345 0.71 -1.5 2,924,878 + BT2345 0.71 +0.6 2,924,878 + BT2345 0.71 -4.0 2,924,878 + BT2345 0.71 -2.8 2,924,878 + BT2345 0.71 +0.0 2,924,879 - BT2345 0.71 -2.8 2,924,908 + BT2345 0.76 -0.9 2,924,934 - BT2345 0.80 -2.3 2,924,934 - BT2345 0.80 -0.3 2,924,939 - BT2345 0.81 -2.7 2,924,953 - BT2345 0.83 +2.0 2,924,953 - BT2345 0.83 -1.8 2,924,953 - BT2345 0.83 +1.2 2,924,956 + BT2345 0.83 -1.5 2,924,957 - BT2345 0.83 -0.5 2,924,957 - BT2345 0.83 -2.5 2,924,964 + BT2345 0.85 -0.8 2,924,964 + BT2345 0.85 +0.0 2,924,965 - BT2345 0.85 -2.0 2,924,965 - BT2345 0.85 +2.5 2,925,001 + +0.0 2,925,002 - -1.0 2,925,007 + +1.0 2,925,030 - -1.4 2,925,063 + -1.8 2,925,068 + +0.0 2,925,068 + +0.0 2,925,108 - +1.6 2,925,162 - BT2346 0.12 +0.0 2,925,162 - BT2346 0.12 -1.3 2,925,195 + BT2346 0.17 -3.0 2,925,207 + BT2346 0.19 -1.0 2,925,246 + BT2346 0.25 -0.7 2,925,262 - BT2346 0.27 -1.5 2,925,264 - BT2346 0.27 -1.5 2,925,264 - BT2346 0.27 -2.8 2,925,274 - BT2346 0.29 +1.6 2,925,297 + BT2346 0.32 -0.7 2,925,297 + BT2346 0.32 -2.1 2,925,353 - BT2346 0.41 -1.9
Or see this region's nucleotide sequence