Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2273 and BT2274 are separated by 6 nucleotides BT2274 and BT2275 are separated by 135 nucleotides BT2275 and BT2276 are separated by 18 nucleotides
BT2273: BT2273 - methyltransferase (NCBI ptt file), at 2,854,117 to 2,854,791
BT2273
BT2274: BT2274 - hypothetical protein (NCBI ptt file), at 2,854,798 to 2,855,523
BT2274
BT2275: BT2275 - thymidine kinase (NCBI ptt file), at 2,855,659 to 2,856,258
BT2275
BT2276: BT2276 - putative permease (NCBI ptt file), at 2,856,277 to 2,857,410
BT2276
Position (kb)
2855
2856
2857 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2854.868 kb on - strand at 2854.906 kb on - strand, within BT2274 at 2854.970 kb on - strand, within BT2274 at 2855.018 kb on - strand, within BT2274 at 2855.036 kb on - strand, within BT2274 at 2855.072 kb on - strand, within BT2274 at 2855.249 kb on - strand, within BT2274 at 2855.276 kb on - strand, within BT2274 at 2855.360 kb on - strand, within BT2274 at 2855.399 kb on - strand, within BT2274 at 2855.455 kb on - strand at 2855.494 kb on - strand at 2855.532 kb on - strand at 2855.532 kb on - strand at 2855.535 kb on - strand at 2855.566 kb on - strand at 2855.599 kb on + strand at 2855.632 kb on - strand at 2855.632 kb on - strand at 2855.663 kb on + strand at 2855.685 kb on - strand at 2855.799 kb on - strand, within BT2275 at 2855.805 kb on - strand, within BT2275 at 2855.979 kb on - strand, within BT2275 at 2856.071 kb on + strand, within BT2275 at 2856.071 kb on + strand, within BT2275 at 2856.072 kb on - strand, within BT2275 at 2856.072 kb on - strand, within BT2275 at 2856.174 kb on - strand, within BT2275 at 2856.180 kb on - strand, within BT2275 at 2856.254 kb on + strand at 2856.254 kb on + strand at 2856.255 kb on - strand at 2856.256 kb on + strand at 2856.268 kb on - strand at 2856.275 kb on - strand at 2856.277 kb on - strand at 2856.293 kb on + strand at 2856.309 kb on + strand at 2856.310 kb on - strand at 2856.348 kb on - strand at 2856.350 kb on + strand at 2856.350 kb on + strand at 2856.351 kb on - strand at 2856.351 kb on - strand at 2856.380 kb on + strand at 2856.383 kb on + strand at 2856.384 kb on - strand at 2856.393 kb on + strand, within BT2276 at 2856.428 kb on + strand, within BT2276 at 2856.452 kb on + strand, within BT2276 at 2856.453 kb on - strand, within BT2276 at 2856.469 kb on - strand, within BT2276 at 2856.515 kb on + strand, within BT2276 at 2856.515 kb on + strand, within BT2276 at 2856.522 kb on - strand, within BT2276 at 2856.536 kb on + strand, within BT2276 at 2856.576 kb on - strand, within BT2276 at 2856.582 kb on - strand, within BT2276 at 2856.586 kb on + strand, within BT2276 at 2856.586 kb on + strand, within BT2276 at 2856.648 kb on + strand, within BT2276 at 2856.672 kb on + strand, within BT2276 at 2856.674 kb on + strand, within BT2276 at 2856.675 kb on - strand, within BT2276 at 2856.786 kb on - strand, within BT2276 at 2856.788 kb on + strand, within BT2276 at 2856.791 kb on + strand, within BT2276 at 2856.792 kb on - strand, within BT2276 at 2856.792 kb on - strand, within BT2276 at 2856.792 kb on - strand, within BT2276 at 2856.792 kb on - strand, within BT2276 at 2856.793 kb on + strand, within BT2276 at 2856.805 kb on - strand, within BT2276 at 2856.806 kb on + strand, within BT2276 at 2856.817 kb on + strand, within BT2276 at 2856.827 kb on + strand, within BT2276 at 2856.828 kb on - strand, within BT2276 at 2856.862 kb on + strand, within BT2276 at 2856.867 kb on + strand, within BT2276 at 2856.888 kb on - strand, within BT2276 at 2856.888 kb on - strand, within BT2276 at 2856.896 kb on + strand, within BT2276 at 2856.897 kb on - strand, within BT2276 at 2856.916 kb on + strand, within BT2276 at 2856.916 kb on + strand, within BT2276 at 2856.968 kb on + strand, within BT2276 at 2856.968 kb on + strand, within BT2276 at 2856.978 kb on + strand, within BT2276 at 2856.978 kb on + strand, within BT2276 at 2857.032 kb on + strand, within BT2276 at 2857.033 kb on - strand, within BT2276 at 2857.033 kb on - strand, within BT2276 at 2857.090 kb on - strand, within BT2276 at 2857.166 kb on + strand, within BT2276 at 2857.167 kb on - strand, within BT2276 at 2857.245 kb on + strand, within BT2276 at 2857.246 kb on - strand, within BT2276 at 2857.246 kb on - strand, within BT2276
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 2,854,868 - +0.5 2,854,906 - BT2274 0.15 -3.1 2,854,970 - BT2274 0.24 -3.1 2,855,018 - BT2274 0.30 -4.0 2,855,036 - BT2274 0.33 -1.7 2,855,072 - BT2274 0.38 +1.9 2,855,249 - BT2274 0.62 -0.1 2,855,276 - BT2274 0.66 -0.7 2,855,360 - BT2274 0.77 +0.1 2,855,399 - BT2274 0.83 -0.9 2,855,455 - -1.3 2,855,494 - -2.4 2,855,532 - +0.1 2,855,532 - +1.1 2,855,535 - -0.7 2,855,566 - +0.6 2,855,599 + -0.9 2,855,632 - -2.9 2,855,632 - +0.3 2,855,663 + -1.5 2,855,685 - +2.4 2,855,799 - BT2275 0.23 -2.1 2,855,805 - BT2275 0.24 -0.5 2,855,979 - BT2275 0.53 -0.3 2,856,071 + BT2275 0.69 -0.9 2,856,071 + BT2275 0.69 -3.1 2,856,072 - BT2275 0.69 -0.9 2,856,072 - BT2275 0.69 -1.5 2,856,174 - BT2275 0.86 -2.5 2,856,180 - BT2275 0.87 -2.3 2,856,254 + +0.6 2,856,254 + -0.9 2,856,255 - -1.5 2,856,256 + +0.1 2,856,268 - -2.1 2,856,275 - -1.9 2,856,277 - -1.5 2,856,293 + -0.4 2,856,309 + -0.9 2,856,310 - -1.7 2,856,348 - +2.1 2,856,350 + -3.4 2,856,350 + -1.9 2,856,351 - +0.1 2,856,351 - -4.7 2,856,380 + -0.9 2,856,383 + -0.2 2,856,384 - +1.1 2,856,393 + BT2276 0.10 -1.9 2,856,428 + BT2276 0.13 -1.1 2,856,452 + BT2276 0.15 -1.9 2,856,453 - BT2276 0.16 -0.3 2,856,469 - BT2276 0.17 -0.9 2,856,515 + BT2276 0.21 -4.1 2,856,515 + BT2276 0.21 +1.1 2,856,522 - BT2276 0.22 +0.2 2,856,536 + BT2276 0.23 -2.6 2,856,576 - BT2276 0.26 +2.6 2,856,582 - BT2276 0.27 -0.9 2,856,586 + BT2276 0.27 -2.8 2,856,586 + BT2276 0.27 +1.4 2,856,648 + BT2276 0.33 -2.9 2,856,672 + BT2276 0.35 +1.5 2,856,674 + BT2276 0.35 +2.4 2,856,675 - BT2276 0.35 -0.3 2,856,786 - BT2276 0.45 +0.1 2,856,788 + BT2276 0.45 +1.6 2,856,791 + BT2276 0.45 +1.5 2,856,792 - BT2276 0.45 -0.1 2,856,792 - BT2276 0.45 -1.5 2,856,792 - BT2276 0.45 -2.3 2,856,792 - BT2276 0.45 -3.5 2,856,793 + BT2276 0.46 +2.9 2,856,805 - BT2276 0.47 -1.9 2,856,806 + BT2276 0.47 -1.4 2,856,817 + BT2276 0.48 -0.3 2,856,827 + BT2276 0.49 -2.0 2,856,828 - BT2276 0.49 +3.5 2,856,862 + BT2276 0.52 -1.6 2,856,867 + BT2276 0.52 +0.1 2,856,888 - BT2276 0.54 +0.6 2,856,888 - BT2276 0.54 -1.5 2,856,896 + BT2276 0.55 +2.4 2,856,897 - BT2276 0.55 +2.4 2,856,916 + BT2276 0.56 +2.1 2,856,916 + BT2276 0.56 -1.6 2,856,968 + BT2276 0.61 -2.3 2,856,968 + BT2276 0.61 -0.9 2,856,978 + BT2276 0.62 -4.3 2,856,978 + BT2276 0.62 +0.8 2,857,032 + BT2276 0.67 +1.5 2,857,033 - BT2276 0.67 -0.9 2,857,033 - BT2276 0.67 -0.5 2,857,090 - BT2276 0.72 -3.1 2,857,166 + BT2276 0.78 +1.6 2,857,167 - BT2276 0.78 -1.9 2,857,245 + BT2276 0.85 -3.0 2,857,246 - BT2276 0.85 -0.5 2,857,246 - BT2276 0.85 -2.1
Or see this region's nucleotide sequence