Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2129 and BT2130 are separated by 5 nucleotides BT2130 and BT2131 are separated by 431 nucleotides
BT2129: BT2129 - aspartate--ammonia ligase (NCBI ptt file), at 2,680,909 to 2,681,946
BT2129
BT2130: BT2130 - uracil-DNA glycosylase (NCBI ptt file), at 2,681,952 to 2,682,614
BT2130
BT2131: BT2131 - hypothetical protein (NCBI ptt file), at 2,683,046 to 2,683,396
BT2131
Position (kb)
2681
2682
2683 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2681.091 kb on + strand, within BT2129 at 2681.093 kb on + strand, within BT2129 at 2681.214 kb on + strand, within BT2129 at 2681.218 kb on + strand, within BT2129 at 2681.219 kb on - strand, within BT2129 at 2681.327 kb on + strand, within BT2129 at 2681.349 kb on - strand, within BT2129 at 2681.355 kb on + strand, within BT2129 at 2681.356 kb on - strand, within BT2129 at 2681.356 kb on - strand, within BT2129 at 2681.359 kb on + strand, within BT2129 at 2681.360 kb on - strand, within BT2129 at 2681.383 kb on + strand, within BT2129 at 2681.391 kb on - strand, within BT2129 at 2681.391 kb on - strand, within BT2129 at 2681.401 kb on + strand, within BT2129 at 2681.518 kb on + strand, within BT2129 at 2681.533 kb on - strand, within BT2129 at 2681.594 kb on - strand, within BT2129 at 2681.667 kb on - strand, within BT2129 at 2681.811 kb on + strand, within BT2129 at 2681.811 kb on + strand, within BT2129 at 2681.811 kb on + strand, within BT2129 at 2681.811 kb on + strand, within BT2129 at 2681.844 kb on + strand at 2681.845 kb on - strand at 2681.845 kb on - strand at 2681.845 kb on - strand at 2681.851 kb on + strand at 2681.934 kb on - strand at 2681.934 kb on - strand at 2681.940 kb on + strand at 2681.944 kb on + strand at 2681.951 kb on + strand at 2681.951 kb on + strand at 2681.952 kb on - strand at 2681.966 kb on - strand at 2682.083 kb on + strand, within BT2130 at 2682.131 kb on - strand, within BT2130 at 2682.253 kb on + strand, within BT2130 at 2682.253 kb on + strand, within BT2130 at 2682.254 kb on - strand, within BT2130 at 2682.257 kb on + strand, within BT2130 at 2682.257 kb on + strand, within BT2130 at 2682.258 kb on - strand, within BT2130 at 2682.308 kb on - strand, within BT2130 at 2682.311 kb on - strand, within BT2130 at 2682.450 kb on + strand, within BT2130 at 2682.450 kb on + strand, within BT2130 at 2682.500 kb on + strand, within BT2130 at 2682.500 kb on + strand, within BT2130 at 2682.531 kb on + strand, within BT2130 at 2682.532 kb on - strand, within BT2130 at 2682.532 kb on - strand, within BT2130 at 2682.604 kb on + strand at 2682.605 kb on - strand at 2682.676 kb on + strand at 2682.677 kb on - strand at 2682.679 kb on + strand at 2682.717 kb on - strand at 2682.724 kb on + strand at 2682.725 kb on - strand at 2682.725 kb on - strand at 2682.725 kb on - strand at 2682.727 kb on + strand at 2682.754 kb on + strand at 2682.783 kb on + strand at 2682.783 kb on + strand at 2682.784 kb on - strand at 2682.827 kb on - strand at 2682.874 kb on + strand at 2682.875 kb on - strand at 2682.875 kb on - strand at 2682.890 kb on + strand at 2682.911 kb on - strand at 2682.921 kb on - strand at 2682.990 kb on - strand at 2683.106 kb on + strand, within BT2131 at 2683.132 kb on - strand, within BT2131 at 2683.132 kb on - strand, within BT2131 at 2683.136 kb on - strand, within BT2131 at 2683.144 kb on + strand, within BT2131 at 2683.192 kb on + strand, within BT2131 at 2683.192 kb on + strand, within BT2131 at 2683.214 kb on + strand, within BT2131 at 2683.215 kb on - strand, within BT2131 at 2683.215 kb on - strand, within BT2131 at 2683.274 kb on - strand, within BT2131 at 2683.290 kb on - strand, within BT2131 at 2683.301 kb on + strand, within BT2131 at 2683.304 kb on + strand, within BT2131 at 2683.304 kb on + strand, within BT2131 at 2683.304 kb on + strand, within BT2131 at 2683.305 kb on - strand, within BT2131 at 2683.306 kb on + strand, within BT2131 at 2683.361 kb on - strand, within BT2131 at 2683.394 kb on + strand at 2683.500 kb on - strand at 2683.508 kb on + strand at 2683.557 kb on - strand at 2683.557 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 2,681,091 + BT2129 0.18 +0.0 2,681,093 + BT2129 0.18 -0.8 2,681,214 + BT2129 0.29 -0.5 2,681,218 + BT2129 0.30 -2.0 2,681,219 - BT2129 0.30 -1.5 2,681,327 + BT2129 0.40 +1.4 2,681,349 - BT2129 0.42 -2.2 2,681,355 + BT2129 0.43 +0.0 2,681,356 - BT2129 0.43 -4.0 2,681,356 - BT2129 0.43 -1.0 2,681,359 + BT2129 0.43 -4.5 2,681,360 - BT2129 0.43 -2.0 2,681,383 + BT2129 0.46 -1.5 2,681,391 - BT2129 0.46 +2.6 2,681,391 - BT2129 0.46 -1.7 2,681,401 + BT2129 0.47 +0.0 2,681,518 + BT2129 0.59 -0.9 2,681,533 - BT2129 0.60 -0.3 2,681,594 - BT2129 0.66 -1.0 2,681,667 - BT2129 0.73 -0.1 2,681,811 + BT2129 0.87 +0.0 2,681,811 + BT2129 0.87 -1.7 2,681,811 + BT2129 0.87 -1.0 2,681,811 + BT2129 0.87 -1.0 2,681,844 + -0.6 2,681,845 - +2.4 2,681,845 - -1.3 2,681,845 - -3.0 2,681,851 + +0.4 2,681,934 - -1.2 2,681,934 - -1.2 2,681,940 + -0.5 2,681,944 + -2.0 2,681,951 + -2.5 2,681,951 + +0.8 2,681,952 - -1.0 2,681,966 - +0.0 2,682,083 + BT2130 0.20 -4.4 2,682,131 - BT2130 0.27 -1.2 2,682,253 + BT2130 0.45 -3.3 2,682,253 + BT2130 0.45 -2.3 2,682,254 - BT2130 0.46 -3.8 2,682,257 + BT2130 0.46 -2.0 2,682,257 + BT2130 0.46 -3.8 2,682,258 - BT2130 0.46 -2.5 2,682,308 - BT2130 0.54 +0.0 2,682,311 - BT2130 0.54 +0.0 2,682,450 + BT2130 0.75 +0.0 2,682,450 + BT2130 0.75 -2.9 2,682,500 + BT2130 0.83 +1.8 2,682,500 + BT2130 0.83 -3.4 2,682,531 + BT2130 0.87 -1.4 2,682,532 - BT2130 0.87 -2.5 2,682,532 - BT2130 0.87 +1.6 2,682,604 + -4.3 2,682,605 - +0.0 2,682,676 + -2.0 2,682,677 - -2.0 2,682,679 + +0.7 2,682,717 - +0.6 2,682,724 + -1.2 2,682,725 - +2.6 2,682,725 - -2.3 2,682,725 - +1.6 2,682,727 + +3.0 2,682,754 + -0.4 2,682,783 + -2.4 2,682,783 + -3.5 2,682,784 - -2.7 2,682,827 - -2.3 2,682,874 + +0.0 2,682,875 - -1.9 2,682,875 - -2.8 2,682,890 + +0.4 2,682,911 - +3.2 2,682,921 - -1.9 2,682,990 - -1.5 2,683,106 + BT2131 0.17 +1.0 2,683,132 - BT2131 0.25 -2.8 2,683,132 - BT2131 0.25 +0.0 2,683,136 - BT2131 0.26 -2.3 2,683,144 + BT2131 0.28 +2.0 2,683,192 + BT2131 0.42 -1.0 2,683,192 + BT2131 0.42 -2.5 2,683,214 + BT2131 0.48 +0.3 2,683,215 - BT2131 0.48 -1.0 2,683,215 - BT2131 0.48 +0.5 2,683,274 - BT2131 0.65 -1.5 2,683,290 - BT2131 0.70 -1.5 2,683,301 + BT2131 0.73 +1.0 2,683,304 + BT2131 0.74 +4.4 2,683,304 + BT2131 0.74 -4.0 2,683,304 + BT2131 0.74 -0.8 2,683,305 - BT2131 0.74 +4.1 2,683,306 + BT2131 0.74 +0.8 2,683,361 - BT2131 0.90 +1.0 2,683,394 + -2.5 2,683,500 - +0.4 2,683,508 + +1.0 2,683,557 - -1.5 2,683,557 - -3.4
Or see this region's nucleotide sequence