Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1823 and BT1824 overlap by 17 nucleotides BT1824 and BT1825 overlap by 14 nucleotides BT1825 and BT1826 are separated by 399 nucleotides
BT1823: BT1823 - conserved hypothetical protein, putative membrane protein (NCBI ptt file), at 2,284,322 to 2,285,239
BT1823
BT1824: BT1824 - conserved hypothetical protein, putative permease (NCBI ptt file), at 2,285,223 to 2,286,179
BT1824
BT1825: BT1825 - conserved hypothetical protein (NCBI ptt file), at 2,286,166 to 2,286,672
BT1825
BT1826: BT1826 - conserved hypothetical protein (NCBI ptt file), at 2,287,072 to 2,290,188
BT1826
Position (kb)
2286
2287 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2285.193 kb on - strand at 2285.237 kb on + strand at 2285.357 kb on + strand, within BT1824 at 2285.369 kb on + strand, within BT1824 at 2285.370 kb on - strand, within BT1824 at 2285.378 kb on - strand, within BT1824 at 2285.448 kb on - strand, within BT1824 at 2285.515 kb on + strand, within BT1824 at 2285.516 kb on - strand, within BT1824 at 2285.574 kb on + strand, within BT1824 at 2285.649 kb on - strand, within BT1824 at 2285.651 kb on - strand, within BT1824 at 2285.690 kb on - strand, within BT1824 at 2285.700 kb on - strand, within BT1824 at 2285.730 kb on - strand, within BT1824 at 2285.818 kb on + strand, within BT1824 at 2285.911 kb on - strand, within BT1824 at 2285.955 kb on + strand, within BT1824 at 2286.004 kb on - strand, within BT1824 at 2286.178 kb on - strand at 2286.278 kb on + strand, within BT1825 at 2286.326 kb on - strand, within BT1825 at 2286.326 kb on - strand, within BT1825 at 2286.327 kb on + strand, within BT1825 at 2286.341 kb on - strand, within BT1825 at 2286.434 kb on + strand, within BT1825 at 2286.575 kb on - strand, within BT1825 at 2286.575 kb on - strand, within BT1825 at 2286.590 kb on + strand, within BT1825 at 2286.594 kb on + strand, within BT1825 at 2286.670 kb on + strand at 2286.671 kb on - strand at 2286.671 kb on - strand at 2286.671 kb on - strand at 2286.673 kb on + strand at 2286.718 kb on - strand at 2286.718 kb on - strand at 2286.752 kb on - strand at 2286.757 kb on - strand at 2286.789 kb on - strand at 2286.793 kb on + strand at 2286.799 kb on + strand at 2286.800 kb on - strand at 2286.800 kb on - strand at 2286.810 kb on + strand at 2286.853 kb on + strand at 2286.853 kb on + strand at 2286.853 kb on + strand at 2286.854 kb on - strand at 2286.857 kb on + strand at 2286.858 kb on - strand at 2286.858 kb on - strand at 2286.883 kb on + strand at 2286.889 kb on + strand at 2286.889 kb on + strand at 2286.892 kb on + strand at 2286.988 kb on + strand at 2286.989 kb on - strand at 2287.042 kb on - strand at 2287.067 kb on + strand at 2287.068 kb on - strand at 2287.114 kb on - strand at 2287.125 kb on + strand at 2287.155 kb on + strand at 2287.156 kb on - strand at 2287.168 kb on + strand at 2287.245 kb on + strand at 2287.257 kb on + strand at 2287.257 kb on + strand at 2287.258 kb on - strand at 2287.278 kb on + strand at 2287.278 kb on + strand at 2287.279 kb on - strand at 2287.283 kb on - strand at 2287.288 kb on - strand at 2287.291 kb on + strand at 2287.311 kb on - strand at 2287.312 kb on + strand at 2287.313 kb on - strand at 2287.313 kb on - strand at 2287.317 kb on + strand at 2287.326 kb on + strand at 2287.342 kb on - strand at 2287.384 kb on - strand, within BT1826 at 2287.389 kb on + strand, within BT1826 at 2287.389 kb on + strand, within BT1826 at 2287.392 kb on - strand, within BT1826 at 2287.435 kb on + strand, within BT1826 at 2287.436 kb on - strand, within BT1826 at 2287.525 kb on - strand, within BT1826 at 2287.525 kb on - strand, within BT1826 at 2287.525 kb on - strand, within BT1826 at 2287.532 kb on - strand, within BT1826 at 2287.537 kb on - strand, within BT1826 at 2287.537 kb on - strand, within BT1826 at 2287.550 kb on - strand, within BT1826 at 2287.550 kb on - strand, within BT1826 at 2287.568 kb on - strand, within BT1826 at 2287.581 kb on + strand, within BT1826 at 2287.582 kb on - strand, within BT1826 at 2287.626 kb on + strand, within BT1826 at 2287.635 kb on + strand, within BT1826 at 2287.636 kb on - strand, within BT1826 at 2287.636 kb on - strand at 2287.671 kb on + strand, within BT1826 at 2287.671 kb on + strand, within BT1826
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 2,285,193 - -2.1 2,285,237 + -3.0 2,285,357 + BT1824 0.14 -0.8 2,285,369 + BT1824 0.15 +0.8 2,285,370 - BT1824 0.15 -2.5 2,285,378 - BT1824 0.16 -2.9 2,285,448 - BT1824 0.24 -1.1 2,285,515 + BT1824 0.31 -0.5 2,285,516 - BT1824 0.31 -2.9 2,285,574 + BT1824 0.37 +1.0 2,285,649 - BT1824 0.45 -4.2 2,285,651 - BT1824 0.45 -1.0 2,285,690 - BT1824 0.49 -0.7 2,285,700 - BT1824 0.50 -0.5 2,285,730 - BT1824 0.53 -2.9 2,285,818 + BT1824 0.62 +2.7 2,285,911 - BT1824 0.72 +5.0 2,285,955 + BT1824 0.76 +1.1 2,286,004 - BT1824 0.82 -0.2 2,286,178 - +1.2 2,286,278 + BT1825 0.22 -2.9 2,286,326 - BT1825 0.32 +0.8 2,286,326 - BT1825 0.32 -1.5 2,286,327 + BT1825 0.32 -1.2 2,286,341 - BT1825 0.35 -2.5 2,286,434 + BT1825 0.53 -2.2 2,286,575 - BT1825 0.81 +2.1 2,286,575 - BT1825 0.81 -2.2 2,286,590 + BT1825 0.84 -2.6 2,286,594 + BT1825 0.84 -1.9 2,286,670 + -0.7 2,286,671 - -0.6 2,286,671 - +1.3 2,286,671 - -0.9 2,286,673 + +3.3 2,286,718 - -0.5 2,286,718 - -0.9 2,286,752 - +0.8 2,286,757 - -3.6 2,286,789 - +3.9 2,286,793 + -0.9 2,286,799 + +2.0 2,286,800 - +2.4 2,286,800 - -1.8 2,286,810 + -2.2 2,286,853 + -0.9 2,286,853 + -0.7 2,286,853 + -2.5 2,286,854 - +0.1 2,286,857 + +1.7 2,286,858 - +2.8 2,286,858 - -1.2 2,286,883 + -1.0 2,286,889 + +2.1 2,286,889 + +1.4 2,286,892 + +0.1 2,286,988 + +1.1 2,286,989 - +0.1 2,287,042 - +1.1 2,287,067 + +0.5 2,287,068 - +0.8 2,287,114 - -4.6 2,287,125 + -1.5 2,287,155 + -1.9 2,287,156 - -2.5 2,287,168 + -1.2 2,287,245 + -2.0 2,287,257 + -0.9 2,287,257 + -1.9 2,287,258 - -1.7 2,287,278 + +1.1 2,287,278 + +0.1 2,287,279 - +0.7 2,287,283 - +2.7 2,287,288 - +0.1 2,287,291 + -1.5 2,287,311 - -0.3 2,287,312 + -2.1 2,287,313 - -2.2 2,287,313 - -1.1 2,287,317 + +0.1 2,287,326 + -0.9 2,287,342 - -2.2 2,287,384 - BT1826 0.10 +4.0 2,287,389 + BT1826 0.10 -0.9 2,287,389 + BT1826 0.10 -0.9 2,287,392 - BT1826 0.10 -2.8 2,287,435 + BT1826 0.12 +2.0 2,287,436 - BT1826 0.12 -2.4 2,287,525 - BT1826 0.15 -0.4 2,287,525 - BT1826 0.15 -2.9 2,287,525 - BT1826 0.15 +1.1 2,287,532 - BT1826 0.15 -0.3 2,287,537 - BT1826 0.15 -2.6 2,287,537 - BT1826 0.15 -1.2 2,287,550 - BT1826 0.15 +1.1 2,287,550 - BT1826 0.15 -2.1 2,287,568 - BT1826 0.16 +0.9 2,287,581 + BT1826 0.16 -1.5 2,287,582 - BT1826 0.16 +0.1 2,287,626 + BT1826 0.18 +0.1 2,287,635 + BT1826 0.18 -2.5 2,287,636 - BT1826 0.18 -1.9 2,287,636 - -1.5 2,287,671 + BT1826 0.19 +0.4 2,287,671 + BT1826 0.19 -2.2
Or see this region's nucleotide sequence