Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1825

Experiment: BHIS with Trimethoprim 0.00625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1823 and BT1824 overlap by 17 nucleotidesBT1824 and BT1825 overlap by 14 nucleotidesBT1825 and BT1826 are separated by 399 nucleotides BT1823: BT1823 - conserved hypothetical protein, putative membrane protein (NCBI ptt file), at 2,284,322 to 2,285,239 BT1823 BT1824: BT1824 - conserved hypothetical protein, putative permease (NCBI ptt file), at 2,285,223 to 2,286,179 BT1824 BT1825: BT1825 - conserved hypothetical protein (NCBI ptt file), at 2,286,166 to 2,286,672 BT1825 BT1826: BT1826 - conserved hypothetical protein (NCBI ptt file), at 2,287,072 to 2,290,188 BT1826 Position (kb) 2286 2287Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5at 2285.193 kb on - strandat 2285.237 kb on + strandat 2285.357 kb on + strand, within BT1824at 2285.369 kb on + strand, within BT1824at 2285.370 kb on - strand, within BT1824at 2285.378 kb on - strand, within BT1824at 2285.448 kb on - strand, within BT1824at 2285.515 kb on + strand, within BT1824at 2285.516 kb on - strand, within BT1824at 2285.574 kb on + strand, within BT1824at 2285.649 kb on - strand, within BT1824at 2285.651 kb on - strand, within BT1824at 2285.690 kb on - strand, within BT1824at 2285.700 kb on - strand, within BT1824at 2285.730 kb on - strand, within BT1824at 2285.818 kb on + strand, within BT1824at 2285.911 kb on - strand, within BT1824at 2285.955 kb on + strand, within BT1824at 2286.004 kb on - strand, within BT1824at 2286.178 kb on - strandat 2286.278 kb on + strand, within BT1825at 2286.326 kb on - strand, within BT1825at 2286.326 kb on - strand, within BT1825at 2286.327 kb on + strand, within BT1825at 2286.341 kb on - strand, within BT1825at 2286.434 kb on + strand, within BT1825at 2286.575 kb on - strand, within BT1825at 2286.575 kb on - strand, within BT1825at 2286.590 kb on + strand, within BT1825at 2286.594 kb on + strand, within BT1825at 2286.670 kb on + strandat 2286.671 kb on - strandat 2286.671 kb on - strandat 2286.671 kb on - strandat 2286.673 kb on + strandat 2286.718 kb on - strandat 2286.718 kb on - strandat 2286.752 kb on - strandat 2286.757 kb on - strandat 2286.789 kb on - strandat 2286.793 kb on + strandat 2286.799 kb on + strandat 2286.800 kb on - strandat 2286.800 kb on - strandat 2286.810 kb on + strandat 2286.853 kb on + strandat 2286.853 kb on + strandat 2286.853 kb on + strandat 2286.854 kb on - strandat 2286.857 kb on + strandat 2286.858 kb on - strandat 2286.858 kb on - strandat 2286.883 kb on + strandat 2286.889 kb on + strandat 2286.889 kb on + strandat 2286.892 kb on + strandat 2286.988 kb on + strandat 2286.989 kb on - strandat 2287.042 kb on - strandat 2287.067 kb on + strandat 2287.068 kb on - strandat 2287.114 kb on - strandat 2287.125 kb on + strandat 2287.155 kb on + strandat 2287.156 kb on - strandat 2287.168 kb on + strandat 2287.245 kb on + strandat 2287.257 kb on + strandat 2287.257 kb on + strandat 2287.258 kb on - strandat 2287.278 kb on + strandat 2287.278 kb on + strandat 2287.279 kb on - strandat 2287.283 kb on - strandat 2287.288 kb on - strandat 2287.291 kb on + strandat 2287.311 kb on - strandat 2287.312 kb on + strandat 2287.313 kb on - strandat 2287.313 kb on - strandat 2287.317 kb on + strandat 2287.326 kb on + strandat 2287.342 kb on - strandat 2287.384 kb on - strand, within BT1826at 2287.389 kb on + strand, within BT1826at 2287.389 kb on + strand, within BT1826at 2287.392 kb on - strand, within BT1826at 2287.435 kb on + strand, within BT1826at 2287.436 kb on - strand, within BT1826at 2287.525 kb on - strand, within BT1826at 2287.525 kb on - strand, within BT1826at 2287.525 kb on - strand, within BT1826at 2287.532 kb on - strand, within BT1826at 2287.537 kb on - strand, within BT1826at 2287.537 kb on - strand, within BT1826at 2287.550 kb on - strand, within BT1826at 2287.550 kb on - strand, within BT1826at 2287.568 kb on - strand, within BT1826at 2287.581 kb on + strand, within BT1826at 2287.582 kb on - strand, within BT1826at 2287.626 kb on + strand, within BT1826at 2287.635 kb on + strand, within BT1826at 2287.636 kb on - strand, within BT1826at 2287.636 kb on - strandat 2287.671 kb on + strand, within BT1826at 2287.671 kb on + strand, within BT1826

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM
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2,285,193 - -2.1
2,285,237 + -3.0
2,285,357 + BT1824 0.14 -0.8
2,285,369 + BT1824 0.15 +0.8
2,285,370 - BT1824 0.15 -2.5
2,285,378 - BT1824 0.16 -2.9
2,285,448 - BT1824 0.24 -1.1
2,285,515 + BT1824 0.31 -0.5
2,285,516 - BT1824 0.31 -2.9
2,285,574 + BT1824 0.37 +1.0
2,285,649 - BT1824 0.45 -4.2
2,285,651 - BT1824 0.45 -1.0
2,285,690 - BT1824 0.49 -0.7
2,285,700 - BT1824 0.50 -0.5
2,285,730 - BT1824 0.53 -2.9
2,285,818 + BT1824 0.62 +2.7
2,285,911 - BT1824 0.72 +5.0
2,285,955 + BT1824 0.76 +1.1
2,286,004 - BT1824 0.82 -0.2
2,286,178 - +1.2
2,286,278 + BT1825 0.22 -2.9
2,286,326 - BT1825 0.32 +0.8
2,286,326 - BT1825 0.32 -1.5
2,286,327 + BT1825 0.32 -1.2
2,286,341 - BT1825 0.35 -2.5
2,286,434 + BT1825 0.53 -2.2
2,286,575 - BT1825 0.81 +2.1
2,286,575 - BT1825 0.81 -2.2
2,286,590 + BT1825 0.84 -2.6
2,286,594 + BT1825 0.84 -1.9
2,286,670 + -0.7
2,286,671 - -0.6
2,286,671 - +1.3
2,286,671 - -0.9
2,286,673 + +3.3
2,286,718 - -0.5
2,286,718 - -0.9
2,286,752 - +0.8
2,286,757 - -3.6
2,286,789 - +3.9
2,286,793 + -0.9
2,286,799 + +2.0
2,286,800 - +2.4
2,286,800 - -1.8
2,286,810 + -2.2
2,286,853 + -0.9
2,286,853 + -0.7
2,286,853 + -2.5
2,286,854 - +0.1
2,286,857 + +1.7
2,286,858 - +2.8
2,286,858 - -1.2
2,286,883 + -1.0
2,286,889 + +2.1
2,286,889 + +1.4
2,286,892 + +0.1
2,286,988 + +1.1
2,286,989 - +0.1
2,287,042 - +1.1
2,287,067 + +0.5
2,287,068 - +0.8
2,287,114 - -4.6
2,287,125 + -1.5
2,287,155 + -1.9
2,287,156 - -2.5
2,287,168 + -1.2
2,287,245 + -2.0
2,287,257 + -0.9
2,287,257 + -1.9
2,287,258 - -1.7
2,287,278 + +1.1
2,287,278 + +0.1
2,287,279 - +0.7
2,287,283 - +2.7
2,287,288 - +0.1
2,287,291 + -1.5
2,287,311 - -0.3
2,287,312 + -2.1
2,287,313 - -2.2
2,287,313 - -1.1
2,287,317 + +0.1
2,287,326 + -0.9
2,287,342 - -2.2
2,287,384 - BT1826 0.10 +4.0
2,287,389 + BT1826 0.10 -0.9
2,287,389 + BT1826 0.10 -0.9
2,287,392 - BT1826 0.10 -2.8
2,287,435 + BT1826 0.12 +2.0
2,287,436 - BT1826 0.12 -2.4
2,287,525 - BT1826 0.15 -0.4
2,287,525 - BT1826 0.15 -2.9
2,287,525 - BT1826 0.15 +1.1
2,287,532 - BT1826 0.15 -0.3
2,287,537 - BT1826 0.15 -2.6
2,287,537 - BT1826 0.15 -1.2
2,287,550 - BT1826 0.15 +1.1
2,287,550 - BT1826 0.15 -2.1
2,287,568 - BT1826 0.16 +0.9
2,287,581 + BT1826 0.16 -1.5
2,287,582 - BT1826 0.16 +0.1
2,287,626 + BT1826 0.18 +0.1
2,287,635 + BT1826 0.18 -2.5
2,287,636 - BT1826 0.18 -1.9
2,287,636 - -1.5
2,287,671 + BT1826 0.19 +0.4
2,287,671 + BT1826 0.19 -2.2

Or see this region's nucleotide sequence