Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1727 and BT1728 are separated by 9 nucleotides BT1728 and BT1729 are separated by 76 nucleotides BT1729 and BT1730 overlap by 19 nucleotides BT1730 and BT1731 are separated by 0 nucleotides
BT1727: BT1727 - putative transmembrane sensor (NCBI ptt file), at 2,126,087 to 2,126,938
BT1727
BT1728: BT1728 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 2,126,948 to 2,127,538
BT1728
BT1729: BT1729 - peptide chain release factor 3 (RF-3) (NCBI ptt file), at 2,127,615 to 2,129,213
BT1729
BT1730: BT1730 - putative dTDP-4-dehydrorhamnose reductase (NCBI ptt file), at 2,129,195 to 2,130,043
BT1730
BT1731: BT1731 - hypothetical protein (NCBI ptt file), at 2,130,044 to 2,130,589
BT1731
Position (kb)
2127
2128
2129
2130 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2126.651 kb on + strand, within BT1727 at 2126.652 kb on - strand, within BT1727 at 2126.665 kb on + strand, within BT1727 at 2126.665 kb on + strand, within BT1727 at 2126.685 kb on + strand, within BT1727 at 2126.688 kb on + strand, within BT1727 at 2126.688 kb on + strand, within BT1727 at 2126.837 kb on + strand, within BT1727 at 2126.905 kb on + strand at 2126.908 kb on - strand at 2126.908 kb on - strand at 2126.908 kb on - strand at 2127.034 kb on + strand, within BT1728 at 2127.034 kb on + strand, within BT1728 at 2127.038 kb on - strand, within BT1728 at 2127.041 kb on - strand, within BT1728 at 2127.101 kb on - strand, within BT1728 at 2127.115 kb on - strand, within BT1728 at 2127.120 kb on - strand at 2127.170 kb on + strand, within BT1728 at 2127.212 kb on + strand, within BT1728 at 2127.212 kb on + strand, within BT1728 at 2127.213 kb on - strand, within BT1728 at 2127.213 kb on - strand, within BT1728 at 2127.213 kb on - strand, within BT1728 at 2127.214 kb on - strand, within BT1728 at 2127.219 kb on - strand, within BT1728 at 2127.235 kb on + strand, within BT1728 at 2127.280 kb on + strand, within BT1728 at 2127.379 kb on - strand, within BT1728 at 2127.385 kb on - strand, within BT1728 at 2127.421 kb on + strand, within BT1728 at 2127.422 kb on - strand, within BT1728 at 2127.532 kb on + strand at 2127.533 kb on - strand at 2127.617 kb on - strand at 2127.642 kb on - strand at 2127.680 kb on - strand at 2127.728 kb on + strand at 2127.736 kb on + strand at 2127.740 kb on + strand at 2127.799 kb on + strand, within BT1729 at 2127.836 kb on - strand, within BT1729 at 2127.836 kb on - strand, within BT1729 at 2127.900 kb on - strand, within BT1729 at 2128.214 kb on - strand, within BT1729 at 2128.223 kb on + strand, within BT1729 at 2128.224 kb on - strand, within BT1729 at 2128.224 kb on - strand, within BT1729 at 2128.228 kb on - strand, within BT1729 at 2128.245 kb on + strand, within BT1729 at 2128.246 kb on - strand, within BT1729 at 2128.246 kb on - strand, within BT1729 at 2128.296 kb on - strand, within BT1729 at 2128.301 kb on + strand, within BT1729 at 2128.360 kb on - strand, within BT1729 at 2128.443 kb on + strand, within BT1729 at 2128.575 kb on + strand, within BT1729 at 2128.576 kb on - strand, within BT1729 at 2128.588 kb on - strand, within BT1729 at 2128.621 kb on + strand, within BT1729 at 2128.622 kb on - strand, within BT1729 at 2128.647 kb on - strand, within BT1729 at 2128.649 kb on + strand, within BT1729 at 2128.651 kb on + strand, within BT1729 at 2128.651 kb on + strand, within BT1729 at 2128.682 kb on + strand, within BT1729 at 2128.864 kb on - strand, within BT1729 at 2128.871 kb on + strand, within BT1729 at 2128.881 kb on + strand, within BT1729 at 2128.895 kb on + strand, within BT1729 at 2128.900 kb on + strand, within BT1729 at 2128.901 kb on - strand, within BT1729 at 2128.943 kb on + strand, within BT1729 at 2128.944 kb on - strand, within BT1729 at 2129.051 kb on + strand, within BT1729 at 2129.124 kb on - strand at 2129.145 kb on - strand at 2129.177 kb on + strand at 2129.189 kb on + strand at 2129.194 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 2,126,651 + BT1727 0.66 +2.8 2,126,652 - BT1727 0.66 -3.1 2,126,665 + BT1727 0.68 -1.1 2,126,665 + BT1727 0.68 -3.0 2,126,685 + BT1727 0.70 -1.0 2,126,688 + BT1727 0.71 +1.0 2,126,688 + BT1727 0.71 -2.4 2,126,837 + BT1727 0.88 +0.0 2,126,905 + -1.8 2,126,908 - -1.3 2,126,908 - -0.4 2,126,908 - -3.1 2,127,034 + BT1728 0.15 +0.5 2,127,034 + BT1728 0.15 +1.0 2,127,038 - BT1728 0.15 +2.0 2,127,041 - BT1728 0.16 -2.5 2,127,101 - BT1728 0.26 +0.6 2,127,115 - BT1728 0.28 +0.6 2,127,120 - +0.0 2,127,170 + BT1728 0.38 +2.0 2,127,212 + BT1728 0.45 -1.3 2,127,212 + BT1728 0.45 -1.0 2,127,213 - BT1728 0.45 +1.0 2,127,213 - BT1728 0.45 +0.0 2,127,213 - BT1728 0.45 -3.9 2,127,214 - BT1728 0.45 -0.4 2,127,219 - BT1728 0.46 +1.8 2,127,235 + BT1728 0.49 -0.2 2,127,280 + BT1728 0.56 +1.5 2,127,379 - BT1728 0.73 -2.0 2,127,385 - BT1728 0.74 -1.8 2,127,421 + BT1728 0.80 -2.0 2,127,422 - BT1728 0.80 -3.4 2,127,532 + -4.8 2,127,533 - +1.3 2,127,617 - -3.5 2,127,642 - -2.0 2,127,680 - -4.7 2,127,728 + -5.2 2,127,736 + -3.5 2,127,740 + -3.2 2,127,799 + BT1729 0.12 -1.5 2,127,836 - BT1729 0.14 -2.8 2,127,836 - BT1729 0.14 -4.4 2,127,900 - BT1729 0.18 -3.3 2,128,214 - BT1729 0.37 -1.3 2,128,223 + BT1729 0.38 +1.0 2,128,224 - BT1729 0.38 +1.6 2,128,224 - BT1729 0.38 -3.8 2,128,228 - BT1729 0.38 -0.4 2,128,245 + BT1729 0.39 -1.0 2,128,246 - BT1729 0.39 -3.5 2,128,246 - BT1729 0.39 -1.5 2,128,296 - BT1729 0.43 -3.3 2,128,301 + BT1729 0.43 -1.8 2,128,360 - BT1729 0.47 -4.1 2,128,443 + BT1729 0.52 -3.3 2,128,575 + BT1729 0.60 -2.0 2,128,576 - BT1729 0.60 -5.7 2,128,588 - BT1729 0.61 -2.0 2,128,621 + BT1729 0.63 -3.0 2,128,622 - BT1729 0.63 -1.5 2,128,647 - BT1729 0.65 -2.8 2,128,649 + BT1729 0.65 -4.9 2,128,651 + BT1729 0.65 -5.1 2,128,651 + BT1729 0.65 -1.5 2,128,682 + BT1729 0.67 -3.0 2,128,864 - BT1729 0.78 -2.0 2,128,871 + BT1729 0.79 -3.5 2,128,881 + BT1729 0.79 -1.0 2,128,895 + BT1729 0.80 -4.2 2,128,900 + BT1729 0.80 -4.2 2,128,901 - BT1729 0.80 +1.6 2,128,943 + BT1729 0.83 -5.1 2,128,944 - BT1729 0.83 -5.1 2,129,051 + BT1729 0.90 -5.2 2,129,124 - -2.3 2,129,145 - -4.0 2,129,177 + -5.1 2,129,189 + -4.0 2,129,194 - -3.3
Or see this region's nucleotide sequence