Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1729

Experiment: BHIS with Trimethoprim 0.00625 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBT1727 and BT1728 are separated by 9 nucleotidesBT1728 and BT1729 are separated by 76 nucleotidesBT1729 and BT1730 overlap by 19 nucleotidesBT1730 and BT1731 are separated by 0 nucleotides BT1727: BT1727 - putative transmembrane sensor (NCBI ptt file), at 2,126,087 to 2,126,938 BT1727 BT1728: BT1728 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 2,126,948 to 2,127,538 BT1728 BT1729: BT1729 - peptide chain release factor 3 (RF-3) (NCBI ptt file), at 2,127,615 to 2,129,213 BT1729 BT1730: BT1730 - putative dTDP-4-dehydrorhamnose reductase (NCBI ptt file), at 2,129,195 to 2,130,043 BT1730 BT1731: BT1731 - hypothetical protein (NCBI ptt file), at 2,130,044 to 2,130,589 BT1731 Position (kb) 2127 2128 2129 2130Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 2126.651 kb on + strand, within BT1727at 2126.652 kb on - strand, within BT1727at 2126.665 kb on + strand, within BT1727at 2126.665 kb on + strand, within BT1727at 2126.685 kb on + strand, within BT1727at 2126.688 kb on + strand, within BT1727at 2126.688 kb on + strand, within BT1727at 2126.837 kb on + strand, within BT1727at 2126.905 kb on + strandat 2126.908 kb on - strandat 2126.908 kb on - strandat 2126.908 kb on - strandat 2127.034 kb on + strand, within BT1728at 2127.034 kb on + strand, within BT1728at 2127.038 kb on - strand, within BT1728at 2127.041 kb on - strand, within BT1728at 2127.101 kb on - strand, within BT1728at 2127.115 kb on - strand, within BT1728at 2127.120 kb on - strandat 2127.170 kb on + strand, within BT1728at 2127.212 kb on + strand, within BT1728at 2127.212 kb on + strand, within BT1728at 2127.213 kb on - strand, within BT1728at 2127.213 kb on - strand, within BT1728at 2127.213 kb on - strand, within BT1728at 2127.214 kb on - strand, within BT1728at 2127.219 kb on - strand, within BT1728at 2127.235 kb on + strand, within BT1728at 2127.280 kb on + strand, within BT1728at 2127.379 kb on - strand, within BT1728at 2127.385 kb on - strand, within BT1728at 2127.421 kb on + strand, within BT1728at 2127.422 kb on - strand, within BT1728at 2127.532 kb on + strandat 2127.533 kb on - strandat 2127.617 kb on - strandat 2127.642 kb on - strandat 2127.680 kb on - strandat 2127.728 kb on + strandat 2127.736 kb on + strandat 2127.740 kb on + strandat 2127.799 kb on + strand, within BT1729at 2127.836 kb on - strand, within BT1729at 2127.836 kb on - strand, within BT1729at 2127.900 kb on - strand, within BT1729at 2128.214 kb on - strand, within BT1729at 2128.223 kb on + strand, within BT1729at 2128.224 kb on - strand, within BT1729at 2128.224 kb on - strand, within BT1729at 2128.228 kb on - strand, within BT1729at 2128.245 kb on + strand, within BT1729at 2128.246 kb on - strand, within BT1729at 2128.246 kb on - strand, within BT1729at 2128.296 kb on - strand, within BT1729at 2128.301 kb on + strand, within BT1729at 2128.360 kb on - strand, within BT1729at 2128.443 kb on + strand, within BT1729at 2128.575 kb on + strand, within BT1729at 2128.576 kb on - strand, within BT1729at 2128.588 kb on - strand, within BT1729at 2128.621 kb on + strand, within BT1729at 2128.622 kb on - strand, within BT1729at 2128.647 kb on - strand, within BT1729at 2128.649 kb on + strand, within BT1729at 2128.651 kb on + strand, within BT1729at 2128.651 kb on + strand, within BT1729at 2128.682 kb on + strand, within BT1729at 2128.864 kb on - strand, within BT1729at 2128.871 kb on + strand, within BT1729at 2128.881 kb on + strand, within BT1729at 2128.895 kb on + strand, within BT1729at 2128.900 kb on + strand, within BT1729at 2128.901 kb on - strand, within BT1729at 2128.943 kb on + strand, within BT1729at 2128.944 kb on - strand, within BT1729at 2129.051 kb on + strand, within BT1729at 2129.124 kb on - strandat 2129.145 kb on - strandat 2129.177 kb on + strandat 2129.189 kb on + strandat 2129.194 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM
remove
2,126,651 + BT1727 0.66 +2.8
2,126,652 - BT1727 0.66 -3.1
2,126,665 + BT1727 0.68 -1.1
2,126,665 + BT1727 0.68 -3.0
2,126,685 + BT1727 0.70 -1.0
2,126,688 + BT1727 0.71 +1.0
2,126,688 + BT1727 0.71 -2.4
2,126,837 + BT1727 0.88 +0.0
2,126,905 + -1.8
2,126,908 - -1.3
2,126,908 - -0.4
2,126,908 - -3.1
2,127,034 + BT1728 0.15 +0.5
2,127,034 + BT1728 0.15 +1.0
2,127,038 - BT1728 0.15 +2.0
2,127,041 - BT1728 0.16 -2.5
2,127,101 - BT1728 0.26 +0.6
2,127,115 - BT1728 0.28 +0.6
2,127,120 - +0.0
2,127,170 + BT1728 0.38 +2.0
2,127,212 + BT1728 0.45 -1.3
2,127,212 + BT1728 0.45 -1.0
2,127,213 - BT1728 0.45 +1.0
2,127,213 - BT1728 0.45 +0.0
2,127,213 - BT1728 0.45 -3.9
2,127,214 - BT1728 0.45 -0.4
2,127,219 - BT1728 0.46 +1.8
2,127,235 + BT1728 0.49 -0.2
2,127,280 + BT1728 0.56 +1.5
2,127,379 - BT1728 0.73 -2.0
2,127,385 - BT1728 0.74 -1.8
2,127,421 + BT1728 0.80 -2.0
2,127,422 - BT1728 0.80 -3.4
2,127,532 + -4.8
2,127,533 - +1.3
2,127,617 - -3.5
2,127,642 - -2.0
2,127,680 - -4.7
2,127,728 + -5.2
2,127,736 + -3.5
2,127,740 + -3.2
2,127,799 + BT1729 0.12 -1.5
2,127,836 - BT1729 0.14 -2.8
2,127,836 - BT1729 0.14 -4.4
2,127,900 - BT1729 0.18 -3.3
2,128,214 - BT1729 0.37 -1.3
2,128,223 + BT1729 0.38 +1.0
2,128,224 - BT1729 0.38 +1.6
2,128,224 - BT1729 0.38 -3.8
2,128,228 - BT1729 0.38 -0.4
2,128,245 + BT1729 0.39 -1.0
2,128,246 - BT1729 0.39 -3.5
2,128,246 - BT1729 0.39 -1.5
2,128,296 - BT1729 0.43 -3.3
2,128,301 + BT1729 0.43 -1.8
2,128,360 - BT1729 0.47 -4.1
2,128,443 + BT1729 0.52 -3.3
2,128,575 + BT1729 0.60 -2.0
2,128,576 - BT1729 0.60 -5.7
2,128,588 - BT1729 0.61 -2.0
2,128,621 + BT1729 0.63 -3.0
2,128,622 - BT1729 0.63 -1.5
2,128,647 - BT1729 0.65 -2.8
2,128,649 + BT1729 0.65 -4.9
2,128,651 + BT1729 0.65 -5.1
2,128,651 + BT1729 0.65 -1.5
2,128,682 + BT1729 0.67 -3.0
2,128,864 - BT1729 0.78 -2.0
2,128,871 + BT1729 0.79 -3.5
2,128,881 + BT1729 0.79 -1.0
2,128,895 + BT1729 0.80 -4.2
2,128,900 + BT1729 0.80 -4.2
2,128,901 - BT1729 0.80 +1.6
2,128,943 + BT1729 0.83 -5.1
2,128,944 - BT1729 0.83 -5.1
2,129,051 + BT1729 0.90 -5.2
2,129,124 - -2.3
2,129,145 - -4.0
2,129,177 + -5.1
2,129,189 + -4.0
2,129,194 - -3.3

Or see this region's nucleotide sequence