Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1479 and BT1480 are separated by 28 nucleotides BT1480 and BT1481 are separated by 51 nucleotides
BT1479: BT1479 - ABC transporter ATP-binding protein (NCBI ptt file), at 1,822,218 to 1,824,086
BT1479
BT1480: BT1480 - hypothetical protein (NCBI ptt file), at 1,824,115 to 1,824,921
BT1480
BT1481: BT1481 - hypothetical protein (NCBI ptt file), at 1,824,973 to 1,826,166
BT1481
Position (kb)
1824
1825 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1823.120 kb on - strand, within BT1479 at 1823.165 kb on - strand, within BT1479 at 1823.233 kb on + strand, within BT1479 at 1823.233 kb on + strand, within BT1479 at 1823.233 kb on + strand, within BT1479 at 1823.234 kb on - strand, within BT1479 at 1823.312 kb on - strand, within BT1479 at 1823.312 kb on - strand, within BT1479 at 1823.331 kb on + strand, within BT1479 at 1823.386 kb on + strand, within BT1479 at 1823.396 kb on + strand, within BT1479 at 1823.397 kb on - strand, within BT1479 at 1823.458 kb on + strand, within BT1479 at 1823.507 kb on - strand, within BT1479 at 1823.655 kb on - strand, within BT1479 at 1823.699 kb on - strand, within BT1479 at 1823.872 kb on + strand, within BT1479 at 1823.873 kb on - strand, within BT1479 at 1823.947 kb on - strand at 1824.063 kb on + strand at 1824.079 kb on - strand at 1824.101 kb on - strand at 1824.101 kb on - strand at 1824.310 kb on + strand, within BT1480 at 1824.311 kb on - strand, within BT1480 at 1824.311 kb on - strand, within BT1480 at 1824.313 kb on - strand, within BT1480 at 1824.436 kb on - strand, within BT1480 at 1824.439 kb on + strand, within BT1480 at 1824.439 kb on + strand, within BT1480 at 1824.439 kb on + strand, within BT1480 at 1824.439 kb on + strand, within BT1480 at 1824.443 kb on - strand, within BT1480 at 1824.470 kb on + strand, within BT1480 at 1824.481 kb on + strand, within BT1480 at 1824.642 kb on + strand, within BT1480 at 1824.642 kb on + strand, within BT1480 at 1824.647 kb on + strand, within BT1480 at 1824.647 kb on + strand, within BT1480 at 1824.647 kb on + strand, within BT1480 at 1824.648 kb on - strand, within BT1480 at 1824.648 kb on - strand, within BT1480 at 1824.680 kb on + strand, within BT1480 at 1824.680 kb on + strand, within BT1480 at 1824.681 kb on - strand, within BT1480 at 1824.761 kb on + strand, within BT1480 at 1824.761 kb on + strand, within BT1480 at 1824.778 kb on - strand, within BT1480 at 1824.789 kb on + strand, within BT1480 at 1824.790 kb on - strand, within BT1480 at 1824.790 kb on - strand, within BT1480 at 1824.809 kb on - strand, within BT1480 at 1825.031 kb on + strand at 1825.032 kb on - strand at 1825.088 kb on + strand at 1825.189 kb on + strand, within BT1481 at 1825.230 kb on + strand, within BT1481 at 1825.231 kb on - strand, within BT1481 at 1825.251 kb on - strand, within BT1481 at 1825.275 kb on + strand, within BT1481 at 1825.280 kb on - strand, within BT1481 at 1825.449 kb on + strand, within BT1481 at 1825.649 kb on + strand, within BT1481 at 1825.674 kb on + strand, within BT1481 at 1825.694 kb on - strand, within BT1481 at 1825.694 kb on - strand, within BT1481 at 1825.739 kb on - strand, within BT1481 at 1825.806 kb on + strand, within BT1481
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 1,823,120 - BT1479 0.48 +2.1 1,823,165 - BT1479 0.51 +2.1 1,823,233 + BT1479 0.54 -2.5 1,823,233 + BT1479 0.54 +0.1 1,823,233 + BT1479 0.54 -1.0 1,823,234 - BT1479 0.54 -2.9 1,823,312 - BT1479 0.59 +1.0 1,823,312 - BT1479 0.59 -1.9 1,823,331 + BT1479 0.60 -1.1 1,823,386 + BT1479 0.62 -0.1 1,823,396 + BT1479 0.63 +2.7 1,823,397 - BT1479 0.63 +1.2 1,823,458 + BT1479 0.66 -0.0 1,823,507 - BT1479 0.69 +0.3 1,823,655 - BT1479 0.77 -2.7 1,823,699 - BT1479 0.79 +0.3 1,823,872 + BT1479 0.88 -1.7 1,823,873 - BT1479 0.89 +1.9 1,823,947 - -0.6 1,824,063 + -0.6 1,824,079 - +0.9 1,824,101 - -1.1 1,824,101 - +0.9 1,824,310 + BT1480 0.24 -1.8 1,824,311 - BT1480 0.24 -2.6 1,824,311 - BT1480 0.24 -1.1 1,824,313 - BT1480 0.25 -1.7 1,824,436 - BT1480 0.40 +0.6 1,824,439 + BT1480 0.40 -1.8 1,824,439 + BT1480 0.40 -0.1 1,824,439 + BT1480 0.40 -1.2 1,824,439 + BT1480 0.40 -2.3 1,824,443 - BT1480 0.41 +0.9 1,824,470 + BT1480 0.44 -3.8 1,824,481 + BT1480 0.45 -1.6 1,824,642 + BT1480 0.65 +1.1 1,824,642 + BT1480 0.65 +2.5 1,824,647 + BT1480 0.66 +1.7 1,824,647 + BT1480 0.66 +1.5 1,824,647 + BT1480 0.66 +1.5 1,824,648 - BT1480 0.66 -2.8 1,824,648 - BT1480 0.66 -1.9 1,824,680 + BT1480 0.70 -2.9 1,824,680 + BT1480 0.70 -4.5 1,824,681 - BT1480 0.70 +0.6 1,824,761 + BT1480 0.80 -3.5 1,824,761 + BT1480 0.80 +1.5 1,824,778 - BT1480 0.82 -2.0 1,824,789 + BT1480 0.84 -4.2 1,824,790 - BT1480 0.84 -2.7 1,824,790 - BT1480 0.84 -4.5 1,824,809 - BT1480 0.86 +3.1 1,825,031 + -3.1 1,825,032 - -2.7 1,825,088 + -0.1 1,825,189 + BT1481 0.18 -1.6 1,825,230 + BT1481 0.22 -1.7 1,825,231 - BT1481 0.22 -0.1 1,825,251 - BT1481 0.23 -0.1 1,825,275 + BT1481 0.25 +0.2 1,825,280 - BT1481 0.26 +0.3 1,825,449 + BT1481 0.40 -0.1 1,825,649 + BT1481 0.57 -0.5 1,825,674 + BT1481 0.59 -1.1 1,825,694 - BT1481 0.60 +0.2 1,825,694 - BT1481 0.60 -0.9 1,825,739 - BT1481 0.64 +0.9 1,825,806 + BT1481 0.70 -1.7
Or see this region's nucleotide sequence