Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1321 and BT1322 are separated by 7 nucleotides BT1322 and BT1323 are separated by 1 nucleotides BT1323 and BT1324 are separated by 176 nucleotides
BT1321: BT1321 - putative phosphate transport ATP-binding protein (NCBI ptt file), at 1,646,268 to 1,647,026
BT1321
BT1322: BT1322 - putative ABC transporter permease protein (NCBI ptt file), at 1,647,034 to 1,647,909
BT1322
BT1323: BT1323 - putative ABC transporter permease protein (NCBI ptt file), at 1,647,911 to 1,649,107
BT1323
BT1324: BT1324 - phosphate ABC transporter, phosphate-binding protein (NCBI ptt file), at 1,649,284 to 1,650,096
BT1324
Position (kb)
1647
1648
1649
1650 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1646.984 kb on - strand at 1647.026 kb on + strand at 1647.131 kb on + strand, within BT1322 at 1647.132 kb on - strand, within BT1322 at 1647.132 kb on - strand, within BT1322 at 1647.142 kb on + strand, within BT1322 at 1647.142 kb on + strand, within BT1322 at 1647.183 kb on - strand, within BT1322 at 1647.204 kb on + strand, within BT1322 at 1647.245 kb on + strand, within BT1322 at 1647.307 kb on + strand, within BT1322 at 1647.790 kb on + strand, within BT1322 at 1647.792 kb on + strand, within BT1322 at 1647.829 kb on - strand at 1647.894 kb on + strand at 1647.920 kb on - strand at 1647.945 kb on + strand at 1647.945 kb on + strand at 1647.968 kb on + strand at 1647.968 kb on + strand at 1647.986 kb on + strand at 1648.210 kb on + strand, within BT1323 at 1648.216 kb on + strand, within BT1323 at 1648.218 kb on + strand, within BT1323 at 1648.218 kb on + strand, within BT1323 at 1648.218 kb on + strand, within BT1323 at 1648.220 kb on + strand, within BT1323 at 1648.315 kb on + strand, within BT1323 at 1648.342 kb on + strand, within BT1323 at 1648.444 kb on + strand, within BT1323 at 1648.517 kb on - strand, within BT1323 at 1648.618 kb on + strand, within BT1323 at 1648.667 kb on - strand, within BT1323 at 1648.669 kb on + strand, within BT1323 at 1648.669 kb on + strand, within BT1323 at 1648.708 kb on + strand, within BT1323 at 1648.779 kb on + strand, within BT1323 at 1648.810 kb on + strand, within BT1323 at 1648.812 kb on + strand, within BT1323 at 1648.910 kb on + strand, within BT1323 at 1648.966 kb on - strand, within BT1323 at 1648.987 kb on + strand, within BT1323 at 1649.041 kb on + strand at 1649.041 kb on + strand at 1649.044 kb on + strand at 1649.044 kb on + strand at 1649.081 kb on + strand at 1649.107 kb on + strand at 1649.107 kb on + strand at 1649.108 kb on - strand at 1649.111 kb on + strand at 1649.574 kb on - strand, within BT1324 at 1649.705 kb on + strand, within BT1324 at 1649.737 kb on - strand, within BT1324 at 1649.876 kb on + strand, within BT1324 at 1649.983 kb on + strand, within BT1324 at 1649.984 kb on - strand, within BT1324 at 1649.984 kb on - strand, within BT1324 at 1649.986 kb on + strand, within BT1324 at 1649.989 kb on + strand, within BT1324 at 1649.992 kb on + strand, within BT1324 at 1649.992 kb on + strand, within BT1324 at 1649.993 kb on - strand, within BT1324 at 1649.995 kb on - strand, within BT1324 at 1650.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 1,646,984 - -0.6 1,647,026 + -2.7 1,647,131 + BT1322 0.11 +0.7 1,647,132 - BT1322 0.11 +3.5 1,647,132 - BT1322 0.11 -1.7 1,647,142 + BT1322 0.12 -2.3 1,647,142 + BT1322 0.12 -0.4 1,647,183 - BT1322 0.17 -0.5 1,647,204 + BT1322 0.19 -1.7 1,647,245 + BT1322 0.24 -0.2 1,647,307 + BT1322 0.31 +2.4 1,647,790 + BT1322 0.86 -0.3 1,647,792 + BT1322 0.87 +2.2 1,647,829 - +1.4 1,647,894 + -2.5 1,647,920 - -0.2 1,647,945 + -1.0 1,647,945 + -2.2 1,647,968 + -1.6 1,647,968 + -0.7 1,647,986 + -0.6 1,648,210 + BT1323 0.25 +1.4 1,648,216 + BT1323 0.25 -2.5 1,648,218 + BT1323 0.26 +2.8 1,648,218 + BT1323 0.26 +2.3 1,648,218 + BT1323 0.26 -2.0 1,648,220 + BT1323 0.26 -1.1 1,648,315 + BT1323 0.34 +2.4 1,648,342 + BT1323 0.36 +2.4 1,648,444 + BT1323 0.45 +0.8 1,648,517 - BT1323 0.51 +1.6 1,648,618 + BT1323 0.59 -2.3 1,648,667 - BT1323 0.63 +1.8 1,648,669 + BT1323 0.63 -2.7 1,648,669 + BT1323 0.63 +1.1 1,648,708 + BT1323 0.67 -2.7 1,648,779 + BT1323 0.73 -0.7 1,648,810 + BT1323 0.75 -0.2 1,648,812 + BT1323 0.75 +3.0 1,648,910 + BT1323 0.83 -1.6 1,648,966 - BT1323 0.88 -2.2 1,648,987 + BT1323 0.90 +2.2 1,649,041 + -0.6 1,649,041 + -1.2 1,649,044 + -0.2 1,649,044 + +0.6 1,649,081 + +0.8 1,649,107 + -1.2 1,649,107 + -1.6 1,649,108 - -2.7 1,649,111 + +1.2 1,649,574 - BT1324 0.36 -0.6 1,649,705 + BT1324 0.52 -1.2 1,649,737 - BT1324 0.56 -1.2 1,649,876 + BT1324 0.73 -1.0 1,649,983 + BT1324 0.86 -1.2 1,649,984 - BT1324 0.86 -1.2 1,649,984 - BT1324 0.86 -3.2 1,649,986 + BT1324 0.86 -0.3 1,649,989 + BT1324 0.87 +0.8 1,649,992 + BT1324 0.87 -1.7 1,649,992 + BT1324 0.87 -1.2 1,649,993 - BT1324 0.87 -0.2 1,649,995 - BT1324 0.87 -3.5 1,650,064 - -0.6
Or see this region's nucleotide sequence