Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1205 and BT1206 are separated by 36 nucleotides BT1206 and BT1207 are separated by 33 nucleotides BT1207 and BT1208 are separated by 127 nucleotides BT1208 and BT1209 are separated by 173 nucleotides
BT1205: BT1205 - putative ATPase, AAA family (NCBI ptt file), at 1,501,726 to 1,502,994
BT1205
BT1206: BT1206 - conserved hypothetical protein (NCBI ptt file), at 1,503,031 to 1,503,393
BT1206
BT1207: BT1207 - glycerate dehydrogenase (NADH-dependent) (NCBI ptt file), at 1,503,427 to 1,504,383
BT1207
BT1208: BT1208 - conserved hypothetical protein (NCBI ptt file), at 1,504,511 to 1,504,891
BT1208
BT1209: BT1209 - cytochrome D ubiquinol oxidase subunit II (NCBI ptt file), at 1,505,065 to 1,506,207
BT1209
Position (kb)
1503
1504
1505 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1502.441 kb on - strand, within BT1205 at 1502.583 kb on - strand, within BT1205 at 1502.754 kb on - strand, within BT1205 at 1502.858 kb on - strand, within BT1205 at 1502.992 kb on + strand at 1502.993 kb on - strand at 1502.997 kb on - strand at 1502.999 kb on + strand at 1503.007 kb on - strand at 1503.011 kb on - strand at 1503.090 kb on - strand, within BT1206 at 1503.093 kb on + strand, within BT1206 at 1503.094 kb on - strand, within BT1206 at 1503.143 kb on + strand, within BT1206 at 1503.144 kb on - strand, within BT1206 at 1503.220 kb on + strand, within BT1206 at 1503.289 kb on - strand, within BT1206 at 1503.289 kb on - strand, within BT1206 at 1503.394 kb on + strand at 1503.406 kb on - strand at 1503.408 kb on - strand at 1503.419 kb on + strand at 1503.507 kb on + strand at 1503.630 kb on + strand, within BT1207 at 1503.690 kb on + strand, within BT1207 at 1503.691 kb on - strand, within BT1207 at 1503.694 kb on - strand, within BT1207 at 1503.705 kb on + strand, within BT1207 at 1503.717 kb on + strand, within BT1207 at 1503.776 kb on + strand, within BT1207 at 1503.819 kb on + strand, within BT1207 at 1503.904 kb on + strand, within BT1207 at 1503.907 kb on - strand, within BT1207 at 1503.909 kb on + strand, within BT1207 at 1503.910 kb on - strand, within BT1207 at 1504.118 kb on + strand, within BT1207 at 1504.270 kb on - strand, within BT1207 at 1504.321 kb on - strand at 1504.346 kb on - strand at 1504.346 kb on - strand at 1504.390 kb on - strand at 1504.444 kb on + strand at 1504.445 kb on - strand at 1504.445 kb on - strand at 1504.452 kb on - strand at 1504.483 kb on - strand at 1504.492 kb on - strand at 1504.494 kb on + strand at 1504.494 kb on + strand at 1504.649 kb on + strand, within BT1208 at 1504.708 kb on + strand, within BT1208 at 1504.708 kb on + strand, within BT1208 at 1504.715 kb on - strand, within BT1208 at 1504.725 kb on + strand, within BT1208 at 1504.754 kb on - strand, within BT1208 at 1504.772 kb on - strand, within BT1208 at 1504.834 kb on - strand, within BT1208 at 1504.880 kb on - strand at 1504.900 kb on - strand at 1504.927 kb on + strand at 1504.939 kb on + strand at 1504.941 kb on + strand at 1504.941 kb on + strand at 1504.942 kb on - strand at 1504.946 kb on - strand at 1504.964 kb on + strand at 1504.995 kb on + strand at 1505.050 kb on + strand at 1505.062 kb on + strand at 1505.122 kb on - strand at 1505.142 kb on + strand at 1505.143 kb on - strand at 1505.143 kb on - strand at 1505.144 kb on + strand at 1505.170 kb on - strand at 1505.177 kb on + strand at 1505.196 kb on + strand, within BT1209 at 1505.250 kb on - strand, within BT1209 at 1505.259 kb on - strand, within BT1209 at 1505.275 kb on - strand, within BT1209 at 1505.317 kb on - strand, within BT1209 at 1505.317 kb on - strand, within BT1209 at 1505.317 kb on - strand, within BT1209 at 1505.317 kb on - strand, within BT1209 at 1505.320 kb on + strand, within BT1209
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 1,502,441 - BT1205 0.56 +0.3 1,502,583 - BT1205 0.68 -2.8 1,502,754 - BT1205 0.81 -2.1 1,502,858 - BT1205 0.89 -0.3 1,502,992 + -0.3 1,502,993 - -1.8 1,502,997 - +0.7 1,502,999 + +0.7 1,503,007 - -2.6 1,503,011 - -1.5 1,503,090 - BT1206 0.16 -1.8 1,503,093 + BT1206 0.17 -1.3 1,503,094 - BT1206 0.17 +1.7 1,503,143 + BT1206 0.31 -0.8 1,503,144 - BT1206 0.31 -0.8 1,503,220 + BT1206 0.52 +0.8 1,503,289 - BT1206 0.71 -2.4 1,503,289 - BT1206 0.71 -1.3 1,503,394 + +2.9 1,503,406 - -3.6 1,503,408 - -1.3 1,503,419 + -1.7 1,503,507 + -2.8 1,503,630 + BT1207 0.21 +2.3 1,503,690 + BT1207 0.27 +0.3 1,503,691 - BT1207 0.28 +0.7 1,503,694 - BT1207 0.28 +1.3 1,503,705 + BT1207 0.29 -1.0 1,503,717 + BT1207 0.30 +0.7 1,503,776 + BT1207 0.36 +2.1 1,503,819 + BT1207 0.41 -0.3 1,503,904 + BT1207 0.50 -0.3 1,503,907 - BT1207 0.50 -1.3 1,503,909 + BT1207 0.50 +0.7 1,503,910 - BT1207 0.50 -0.7 1,504,118 + BT1207 0.72 -1.1 1,504,270 - BT1207 0.88 -0.3 1,504,321 - -3.5 1,504,346 - -1.8 1,504,346 - -1.5 1,504,390 - -2.7 1,504,444 + -0.0 1,504,445 - -0.7 1,504,445 - -3.9 1,504,452 - -0.2 1,504,483 - -3.1 1,504,492 - -3.6 1,504,494 + +2.0 1,504,494 + +0.8 1,504,649 + BT1208 0.36 -1.2 1,504,708 + BT1208 0.52 +1.3 1,504,708 + BT1208 0.52 +0.8 1,504,715 - BT1208 0.54 -4.0 1,504,725 + BT1208 0.56 -0.2 1,504,754 - BT1208 0.64 -2.2 1,504,772 - BT1208 0.69 +0.8 1,504,834 - BT1208 0.85 +1.3 1,504,880 - -2.1 1,504,900 - +0.4 1,504,927 + -2.1 1,504,939 + -2.6 1,504,941 + -2.8 1,504,941 + -0.7 1,504,942 - -3.1 1,504,946 - -0.2 1,504,964 + -1.4 1,504,995 + +0.8 1,505,050 + -1.1 1,505,062 + +0.3 1,505,122 - -2.5 1,505,142 + -0.9 1,505,143 - +1.2 1,505,143 - -1.5 1,505,144 + -1.4 1,505,170 - +0.0 1,505,177 + -0.2 1,505,196 + BT1209 0.11 -1.6 1,505,250 - BT1209 0.16 -2.4 1,505,259 - BT1209 0.17 -3.4 1,505,275 - BT1209 0.18 -2.1 1,505,317 - BT1209 0.22 -2.2 1,505,317 - BT1209 0.22 +2.3 1,505,317 - BT1209 0.22 -1.4 1,505,317 - BT1209 0.22 -2.6 1,505,320 + BT1209 0.22 +1.5
Or see this region's nucleotide sequence